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Aliases for UBE2M Gene

Aliases for UBE2M Gene

  • Ubiquitin Conjugating Enzyme E2 M 2 3 5
  • Ubiquitin-Conjugating Enzyme E2M (UBC12 Homolog, Yeast) 2 3
  • NEDD8 Carrier Protein 3 4
  • UBC12 3 4
  • Ubiquitin-Conjugating Enzyme E2M (Homologous To Yeast UBC12) 2
  • Ubiquitin-Conjugating Enzyme E2 M 4
  • Ubiquitin Conjugating Enzyme E2M 3
  • Ubiquitin-Conjugating Enzyme E2M 2
  • NEDD8-Conjugating Enzyme Ubc12 3
  • Ubiquitin Carrier Protein M 3
  • Ubiquitin-Protein Ligase M 3
  • NEDD8 Protein Ligase 3
  • Yeast UBC12 Homolog 3
  • EC 2.3.2.- 4
  • UBC-RS2 3
  • HUbc12 3

External Ids for UBE2M Gene

Previous GeneCards Identifiers for UBE2M Gene

  • GC19M059965
  • GC19M059521
  • GC19M063742
  • GC19M063758
  • GC19M059067

Summaries for UBE2M Gene

Entrez Gene Summary for UBE2M Gene

  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]

GeneCards Summary for UBE2M Gene

UBE2M (Ubiquitin Conjugating Enzyme E2 M) is a Protein Coding gene. Diseases associated with UBE2M include Familial Hypertension. Among its related pathways are CDK-mediated phosphorylation and removal of Cdc6 and Class I MHC mediated antigen processing and presentation. Gene Ontology (GO) annotations related to this gene include ubiquitin-protein transferase activity and ubiquitin protein ligase activity. An important paralog of this gene is UBE2F.

UniProtKB/Swiss-Prot for UBE2M Gene

  • Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

Gene Wiki entry for UBE2M Gene

Additional gene information for UBE2M Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for UBE2M Gene

Genomics for UBE2M Gene

GeneHancer (GH) Regulatory Elements for UBE2M Gene

Promoters and enhancers for UBE2M Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH19J058553 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 650.7 +1.9 1863 7.2 CLOCK FEZF1 DMAP1 IRF4 YY1 SLC30A9 ZNF213 E2F8 ZNF143 SP3 UBE2M CHMP2A TRIM28 ZNF417 ZNF134 ZNF606 ZNF274 ZNF586 MZF1 LOC100128398
GH19J058517 Promoter/Enhancer 2.8 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 13.1 +39.3 39268 4.9 MLX ZFP64 FEZF1 DMAP1 YY1 SLC30A9 ZNF213 E2F8 ZNF416 ZNF143 ZBTB45 PIR40518 SLC27A5 ZNF324B TRIM28 ZNF446 ZNF134 ZNF417 ZNF274 ZNF549
GH19J058580 Enhancer 1.1 Ensembl ENCODE 12.3 -22.3 -22288 1.5 PKNOX1 ATF1 SIN3A GTF3C2 ZNF766 E2F8 ARID2 ZNF143 ATF7 NCOA1 ENSG00000285138 GC19P058668 PIR59692 TRIM28 ZNF274 ZNF417 ZNF587B ZNF587 ZNF586 ENSG00000267216
GH19J058522 Enhancer 0.3 dbSUPER 13.1 +36.1 36055 0.6 ZNF7 TRIM28 CHMP2A UBE2M MZF1 CENPBD1P1 RNU6-1337P ZNF446 ZNF324 ZNF324B PIR40518
GH19J058537 Promoter/Enhancer 1.5 FANTOM5 Ensembl ENCODE 1.6 +20.7 20657 2.3 SOX13 USF1 RB1 BACH1 ETS1 POLR2A NR2F6 SOX5 ZNF687 RNF2 ZBTB45 ZNF584 ZSCAN18 ENSG00000232098 ZNF8 ZNF544 TRIM28 ZNF329 ZNF497 ZNF324
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around UBE2M on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the UBE2M gene promoter:
  • AML1a

Genomic Locations for UBE2M Gene

Genomic Locations for UBE2M Gene
chr19:58,555,712-58,558,976
(GRCh38/hg38)
Size:
3,265 bases
Orientation:
Minus strand
chr19:59,067,079-59,070,343
(GRCh37/hg19)
Size:
3,265 bases
Orientation:
Minus strand

Genomic View for UBE2M Gene

Genes around UBE2M on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
UBE2M Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for UBE2M Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for UBE2M Gene

Proteins for UBE2M Gene

  • Protein details for UBE2M Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P61081-UBC12_HUMAN
    Recommended name:
    NEDD8-conjugating enzyme Ubc12
    Protein Accession:
    P61081
    Secondary Accessions:
    • O76069
    • Q8VC50

    Protein attributes for UBE2M Gene

    Size:
    183 amino acids
    Molecular mass:
    20900 Da
    Quaternary structure:
    • Interacts with UBA3 and RBX1. Interacts (acetylated at N-terminal methionine) with DCUN1D1 (via DCUN1 domain) (PubMed:28581483).

    Three dimensional structures from OCA and Proteopedia for UBE2M Gene

neXtProt entry for UBE2M Gene

Post-translational modifications for UBE2M Gene

  • The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
  • Ubiquitination at isoforms=81, posLast=7575, posLast=3636, isoforms=8, and posLast=33
  • Modification sites at PhosphoSitePlus

Other Protein References for UBE2M Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Abcam antibodies for UBE2M
  • Boster Bio Antibodies for UBE2M

No data available for DME Specific Peptides for UBE2M Gene

Domains & Families for UBE2M Gene

Gene Families for UBE2M Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for UBE2M Gene

Suggested Antigen Peptide Sequences for UBE2M Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P61081

UniProtKB/Swiss-Prot:

UBC12_HUMAN :
  • Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
Domain:
  • Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
Family:
  • Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
genes like me logo Genes that share domains with UBE2M: view

Function for UBE2M Gene

Molecular function for UBE2M Gene

UniProtKB/Swiss-Prot Function:
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

Enzyme Numbers (IUBMB) for UBE2M Gene

Gene Ontology (GO) - Molecular Function for UBE2M Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity TAS 9694792
GO:0005515 protein binding IPI 15361859
GO:0005524 ATP binding IEA --
GO:0016740 transferase activity IEA --
GO:0019788 NEDD8 transferase activity TAS --
genes like me logo Genes that share ontologies with UBE2M: view
genes like me logo Genes that share phenotypes with UBE2M: view

Animal Models for UBE2M Gene

MGI Knock Outs for UBE2M:
  • Ube2m Ube2m<tm1.1(KOMP)Wtsi>

Animal Model Products

CRISPR Products

miRNA for UBE2M Gene

miRTarBase miRNAs that target UBE2M

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for UBE2M

Clone Products

  • Addgene plasmids for UBE2M

No data available for Phenotypes From GWAS Catalog , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for UBE2M Gene

Localization for UBE2M Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for UBE2M gene
Compartment Confidence
extracellular 5
nucleus 5
cytosol 5

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleus (3)
  • Cytosol (2)
  • Nuclear bodies (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for UBE2M Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IBA --
GO:0005829 cytosol TAS --
GO:0070062 extracellular exosome HDA --
genes like me logo Genes that share ontologies with UBE2M: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for UBE2M Gene

Pathways & Interactions for UBE2M Gene

genes like me logo Genes that share pathways with UBE2M: view

UniProtKB/Swiss-Prot P61081-UBC12_HUMAN

  • Pathway: Protein modification; protein neddylation.

Gene Ontology (GO) - Biological Process for UBE2M Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006464 cellular protein modification process TAS 9694792
GO:0007179 transforming growth factor beta receptor signaling pathway TAS --
GO:0016567 protein ubiquitination IEA --
GO:0038061 NIK/NF-kappaB signaling TAS --
GO:0043525 positive regulation of neuron apoptotic process IEA --
genes like me logo Genes that share ontologies with UBE2M: view

No data available for SIGNOR curated interactions for UBE2M Gene

Drugs & Compounds for UBE2M Gene

(3) Drugs for UBE2M Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Adenosine monophosphate Approved, Investigational Nutra 0
ATP Investigational Nutra Agonist, Activator, Full agonist, Antagonist, Potentiation, Pore Blocker 0

(1) Additional Compounds for UBE2M Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • [(ho)2P(O)OP(O)(OH)2]
  • Acide diphosphorique
  • Diphosphorsaeure
  • H4P2O7
  • PYROphosphATE
14000-31-8
genes like me logo Genes that share compounds with UBE2M: view

Transcripts for UBE2M Gene

mRNA/cDNA for UBE2M Gene

(1) REFSEQ mRNAs :
(3) Additional mRNA sequences :
(250) Selected AceView cDNA sequences:
(5) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for UBE2M Gene

Ubiquitin-conjugating enzyme E2M:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for UBE2M

Clone Products

  • Addgene plasmids for UBE2M

Alternative Splicing Database (ASD) splice patterns (SP) for UBE2M Gene

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b
SP1: -
SP2: -
SP3:
SP4:

Relevant External Links for UBE2M Gene

GeneLoc Exon Structure for
UBE2M
ECgene alternative splicing isoforms for
UBE2M

Expression for UBE2M Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for UBE2M Gene

Protein differential expression in normal tissues from HIPED for UBE2M Gene

This gene is overexpressed in Peripheral blood mononuclear cells (7.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for UBE2M Gene



Protein tissue co-expression partners for UBE2M Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of UBE2M Gene:

UBE2M

SOURCE GeneReport for Unigene cluster for UBE2M Gene:

Hs.406068

Evidence on tissue expression from TISSUES for UBE2M Gene

  • Skin(4.3)
  • Nervous system(2.8)
  • Muscle(2.3)
  • Intestine(2.1)
  • Heart(2)
genes like me logo Genes that share expression patterns with UBE2M: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for UBE2M Gene

Orthologs for UBE2M Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for UBE2M Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia UBE2M 34 33
  • 99.77 (n)
OneToOne
dog
(Canis familiaris)
Mammalia UBE2M 34 33
  • 97.27 (n)
OneToOne
cow
(Bos Taurus)
Mammalia UBE2M 34 33
  • 96.54 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ube2m 33
  • 94.9 (n)
mouse
(Mus musculus)
Mammalia Ube2m 16 34 33
  • 94.35 (n)
chicken
(Gallus gallus)
Aves UBE2M 34
  • 47 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia UBE2M 34
  • 98 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia ube2m 33
  • 81.42 (n)
MGC75750 33
zebrafish
(Danio rerio)
Actinopterygii wu:fb58e05 33
  • 81.24 (n)
wufb58e05 33
fruit fly
(Drosophila melanogaster)
Insecta CG7375 35
  • 75 (a)
Ubc12 34 33
  • 73.56 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005916 33
  • 70.13 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ADL035C 33
  • 55.49 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes UBC12 36 34 33
  • 50.93 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0C17248g 33
  • 47.25 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT2G18600 33
  • 59.89 (n)
soybean
(Glycine max)
eudicotyledons Gma.17973 33
rice
(Oryza sativa)
Liliopsida Os08g0374100 33
  • 59.52 (n)
Os.14656 33
barley
(Hordeum vulgare)
Liliopsida Hv.3803 33
corn
(Zea mays)
Liliopsida Zm.4335 33
wheat
(Triticum aestivum)
Liliopsida Ta.3716 33
bread mold
(Neurospora crassa)
Ascomycetes NCU03610 33
  • 62.3 (n)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.4912 34
  • 62 (a)
OneToOne
Cin.1924 33
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes ubc12 33
  • 54.17 (n)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.1924 33
Species where no ortholog for UBE2M was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • worm (Caenorhabditis elegans)

Evolution for UBE2M Gene

ENSEMBL:
Gene Tree for UBE2M (if available)
TreeFam:
Gene Tree for UBE2M (if available)
Aminode:
Evolutionary constrained regions (ECRs) for UBE2M: view image

Paralogs for UBE2M Gene

Paralogs for UBE2M Gene

(20) SIMAP similar genes for UBE2M Gene using alignment to 3 proteins:

  • UBC12_HUMAN
  • M0QX69_HUMAN
  • M0QYI6_HUMAN

Pseudogenes.org Pseudogenes for UBE2M Gene

genes like me logo Genes that share paralogs with UBE2M: view

Variants for UBE2M Gene

Sequence variations from dbSNP and Humsavar for UBE2M Gene

SNP ID Clin Chr 19 pos Variation AA Info Type
rs1000133837 -- 58,555,279(-) C/A downstream_transcript_variant
rs1001524540 -- 58,559,789(-) G/A upstream_transcript_variant
rs1002285561 -- 58,555,552(-) T/A downstream_transcript_variant
rs1002316712 -- 58,555,386(-) T/C downstream_transcript_variant
rs1002934396 -- 58,558,783(-) C/T 5_prime_UTR_variant

Structural Variations from Database of Genomic Variants (DGV) for UBE2M Gene

Variant ID Type Subtype PubMed ID
dgv3687n100 CNV gain 25217958
dgv3688n100 CNV gain 25217958
nsv1057077 CNV gain 25217958
nsv1057206 CNV gain 25217958
nsv1160670 CNV deletion 26073780
nsv515731 CNV gain+loss 19592680
nsv828657 CNV gain 20364138
nsv952156 CNV deletion 24416366

Variation tolerance for UBE2M Gene

Residual Variation Intolerance Score: 49.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.22; 5.00% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for UBE2M Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
UBE2M

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for UBE2M Gene

Disorders for UBE2M Gene

MalaCards: The human disease database

(1) MalaCards diseases for UBE2M Gene - From: GeneCards

Disorder Aliases PubMed IDs
familial hypertension
  • genetic hypertension
- elite association - COSMIC cancer census association via MalaCards
Search UBE2M in MalaCards View complete list of genes associated with diseases

Additional Disease Information for UBE2M

genes like me logo Genes that share disorders with UBE2M: view

No data available for UniProtKB/Swiss-Prot and Genatlas for UBE2M Gene

Publications for UBE2M Gene

  1. A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo. (PMID: 10828074) Wada H … Kamitani T (The Journal of biological chemistry 2000) 3 4 22 58
  2. A new NEDD8-ligating system for cullin-4A. (PMID: 9694792) Osaka F … Kato S (Genes & development 1998) 2 3 4 58
  3. Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase. (PMID: 28581483) Scott DC … Guy RK (Nature chemical biology 2017) 3 4 58
  4. Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes. (PMID: 23201271) Monda JK … Schulman BA (Structure (London, England : 1993) 2013) 3 4 58
  5. N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex. (PMID: 21940857) Scott DC … Schulman BA (Science (New York, N.Y.) 2011) 3 4 58

Products for UBE2M Gene

Sources for UBE2M Gene

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