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Aliases for ZP2 Gene

Aliases for ZP2 Gene

  • Zona Pellucida Glycoprotein 2 2 3 4 5
  • Zona Pellucida Glycoprotein 2 (Sperm Receptor) 2 3
  • Zona Pellucida Protein A 3 4
  • Zp-2 3 4
  • ZPA 3 4
  • Zona Pellucida Sperm-Binding Protein 2 3
  • Zona Pellucida Glycoprotein ZP2 3

External Ids for ZP2 Gene

Previous GeneCards Identifiers for ZP2 Gene

  • GC16M020617
  • GC16M021217
  • GC16M021175
  • GC16M021116
  • GC16M019743
  • GC16M021208

Summaries for ZP2 Gene

Entrez Gene Summary for ZP2 Gene

  • The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

GeneCards Summary for ZP2 Gene

ZP2 (Zona Pellucida Glycoprotein 2) is a Protein Coding gene. Diseases associated with ZP2 include Female Infertility Due To Zona Pellucida Defect and Acheiropody. Among its related pathways are Fertilization and Ovarian Infertility Genes. Gene Ontology (GO) annotations related to this gene include coreceptor activity and acrosin binding. An important paralog of this gene is ZP4.

UniProtKB/Swiss-Prot for ZP2 Gene

  • The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.

Gene Wiki entry for ZP2 Gene

Additional gene information for ZP2 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for ZP2 Gene

Genomics for ZP2 Gene

GeneHancer (GH) Regulatory Elements for ZP2 Gene

Promoters and enhancers for ZP2 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH16J021211 Promoter 0.5 EPDnew 658 +2.9 2915 0.1 ZP2 GC16M021206 ENSG00000262983
GH16J021260 Enhancer 1.2 Ensembl ENCODE 10.4 -47.0 -47010 1.9 PKNOX1 SMAD1 FOXA2 MLX ARNT ARID4B DMAP1 YY1 SLC30A9 ZNF766 CRYM ANKS4B TMEM159 DNAH3 ZP2 SMG1P4 LOC105371123 LOC105371125
GH16J021263 Enhancer 1.1 Ensembl ENCODE 10.4 -49.8 -49839 1.7 HDAC1 ELF3 PKNOX1 FOXA2 ARID4B DMAP1 RAD21 THRB ZNF48 RARA CRYM ANKS4B DNAH3 TMEM159 ZP2 LOC105371123 LOC105371125 GC16M021264
GH16J021202 Enhancer 0.6 ENCODE 0.4 +10.9 10940 2.3 MEIS2 RFX1 RNF2 HLF CEBPB CEBPG CTBP1 HMBOX1 HNF4A DNAH3 TMEM159 ENSG00000262983 ZP2
GH16J021198 Enhancer 0.2 ENCODE 0.4 +14.7 14668 2.1 TMEM159 DNAH3 ENSG00000262983 ZP2
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ZP2 on UCSC Golden Path with GeneCards custom track

Genomic Locations for ZP2 Gene

Genomic Locations for ZP2 Gene
17,061 bases
Minus strand
17,059 bases
Minus strand

Genomic View for ZP2 Gene

Genes around ZP2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ZP2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ZP2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ZP2 Gene

Proteins for ZP2 Gene

  • Protein details for ZP2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Zona pellucida sperm-binding protein 2
    Protein Accession:
    Secondary Accessions:
    • B2R7J2
    • Q4VAN9
    • Q4VAP0
    • Q4VAP1

    Protein attributes for ZP2 Gene

    745 amino acids
    Molecular mass:
    82357 Da
    Quaternary structure:
    • Can form homopolymers that assemble into long fibers (in vitro). Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers (By similarity). Interacts with ZP3 (PubMed:28886344).

    Alternative splice isoforms for ZP2 Gene


neXtProt entry for ZP2 Gene

Post-translational modifications for ZP2 Gene

  • Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
  • Proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL exocytosed from cortical granules after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy. Additional proteolytically cleavage of the N-terminal peptide of 30 kDa occurs in one-cell and two-cell embryos.
  • N-glycosylated.
  • O-glycosylated; contains sulfate-substituted glycans.
  • Glycosylation at isoforms=462, posLast=400400, posLast=269269, isoforms=2223, isoforms=2122, posLast=105105, and posLast=8787
  • Modification sites at PhosphoSitePlus

Other Protein References for ZP2 Gene

No data available for DME Specific Peptides for ZP2 Gene

Domains & Families for ZP2 Gene

Gene Families for ZP2 Gene

Human Protein Atlas (HPA):
  • Predicted membrane proteins

Protein Domains for ZP2 Gene


Suggested Antigen Peptide Sequences for ZP2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
  • The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.
  • Belongs to the ZP domain family. ZPA subfamily.
genes like me logo Genes that share domains with ZP2: view

Function for ZP2 Gene

Molecular function for ZP2 Gene

UniProtKB/Swiss-Prot Function:
The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
GENATLAS Biochemistry:
zona pellucida glycoprotein 2 (sperm receptor)

Gene Ontology (GO) - Molecular Function for ZP2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 23355646
GO:0015026 coreceptor activity TAS 8385033
GO:0032190 acrosin binding IPI 15950651
GO:0042802 identical protein binding IPI 26879157
genes like me logo Genes that share ontologies with ZP2: view
genes like me logo Genes that share phenotypes with ZP2: view

Animal Models for ZP2 Gene

MGI Knock Outs for ZP2:
  • Zp2 Zp2<tm1Dean>

Animal Model Products

Clone Products

No data available for Enzyme Numbers (IUBMB) , Phenotypes From GWAS Catalog , Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for ZP2 Gene

Localization for ZP2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ZP2 Gene

Processed zona pellucida sperm-binding protein 2: Secreted, extracellular space, extracellular matrix. Note=The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida. {ECO:0000250 UniProtKB:P20239}.
Cell membrane; Single-pass type I membrane protein.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ZP2 gene
Compartment Confidence
plasma membrane 5
extracellular 5
endoplasmic reticulum 5
endosome 4
nucleus 2
mitochondrion 1
cytosol 1

Gene Ontology (GO) - Cellular Components for ZP2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region TAS --
GO:0005771 multivesicular body ISS,IEA --
GO:0005783 endoplasmic reticulum IEA,ISS --
GO:0005886 plasma membrane ISS --
GO:0016020 membrane IEA --
genes like me logo Genes that share ontologies with ZP2: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for ZP2 Gene

Pathways & Interactions for ZP2 Gene

genes like me logo Genes that share pathways with ZP2: view

Pathways by source for ZP2 Gene

1 BioSystems pathway for ZP2 Gene

Interacting Proteins for ZP2 Gene

Gene Ontology (GO) - Biological Process for ZP2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007165 signal transduction IEA --
GO:0007338 single fertilization IEA --
GO:0007339 binding of sperm to zona pellucida IEA,TAS --
GO:0060046 NOT regulation of acrosome reaction IDA 11751269
GO:0060468 prevention of polyspermy ISS 22472438
genes like me logo Genes that share ontologies with ZP2: view

No data available for SIGNOR curated interactions for ZP2 Gene

Drugs & Compounds for ZP2 Gene

No Compound Related Data Available

Transcripts for ZP2 Gene

mRNA/cDNA for ZP2 Gene

(3) REFSEQ mRNAs :
(9) Additional mRNA sequences :
(3) Selected AceView cDNA sequences:
(5) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for ZP2 Gene

Zona pellucida glycoprotein 2 (sperm receptor):
Representative Sequences:

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for ZP2 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19
SP1: -
SP2: - -

Relevant External Links for ZP2 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for ZP2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for ZP2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for ZP2 Gene

This gene is overexpressed in Brain - Cerebellar Hemisphere (x25.9) and Brain - Cerebellum (x25.7).

Protein differential expression in normal tissues from HIPED for ZP2 Gene

This gene is overexpressed in Monocytes (32.7), Tlymphocyte (16.7), and Blymphocyte (15.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for ZP2 Gene

Protein tissue co-expression partners for ZP2 Gene

NURSA nuclear receptor signaling pathways regulating expression of ZP2 Gene:


SOURCE GeneReport for Unigene cluster for ZP2 Gene:


Evidence on tissue expression from TISSUES for ZP2 Gene

  • Nervous system(4.3)
genes like me logo Genes that share expression patterns with ZP2: view

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for ZP2 Gene

Orthologs for ZP2 Gene

This gene was present in the common ancestor of chordates.

Orthologs for ZP2 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia ZP2 34 33
  • 99.51 (n)
(Bos Taurus)
Mammalia ZP2 34 33
  • 80.62 (n)
(Canis familiaris)
Mammalia ZP2 34 33
  • 80.17 (n)
(Rattus norvegicus)
Mammalia Zp2 33
  • 73.06 (n)
(Mus musculus)
Mammalia Zp2 16 34 33
  • 72.71 (n)
(Ornithorhynchus anatinus)
Mammalia -- 34
  • 50 (a)
-- 34
  • 44 (a)
(Monodelphis domestica)
Mammalia ZP2 34
  • 49 (a)
(Gallus gallus)
Aves ZP2 34 33
  • 56.51 (n)
(Anolis carolinensis)
Reptilia -- 34
  • 48 (a)
-- 34
  • 47 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia zp2 33
  • 50.25 (n)
African clawed frog
(Xenopus laevis)
Amphibia xlZPA 33
(Danio rerio)
Actinopterygii si:ch211-223m11.2 34
  • 14 (a)
Species where no ortholog for ZP2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for ZP2 Gene

Gene Tree for ZP2 (if available)
Gene Tree for ZP2 (if available)
Evolutionary constrained regions (ECRs) for ZP2: view image

Paralogs for ZP2 Gene

Paralogs for ZP2 Gene

(2) SIMAP similar genes for ZP2 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with ZP2: view

Variants for ZP2 Gene

Sequence variations from dbSNP and Humsavar for ZP2 Gene

SNP ID Clin Chr 16 pos Variation AA Info Type
rs1000253199 -- 21,202,818(-) ACTTAAACTTAA/ACTTAA intron_variant
rs1000321457 -- 21,197,766(-) C/T coding_sequence_variant, missense_variant, non_coding_transcript_variant
rs1000549578 -- 21,208,394(-) G/A intron_variant
rs1001368370 -- 21,201,208(-) /C intron_variant
rs1001461454 -- 21,207,758(-) A/G intron_variant

Structural Variations from Database of Genomic Variants (DGV) for ZP2 Gene

Variant ID Type Subtype PubMed ID
esv3638164 CNV gain 21293372
esv3638165 CNV gain 21293372
nsv1762 CNV insertion 18451855
nsv436835 CNV insertion 17901297
nsv509605 CNV insertion 20534489
nsv524978 CNV loss 19592680
nsv571623 CNV gain 21841781
nsv833166 CNV gain+loss 17160897

Variation tolerance for ZP2 Gene

Residual Variation Intolerance Score: 23.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 7.02; 80.13% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ZP2 Gene

Human Gene Mutation Database (HGMD)
SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ZP2 Gene

Disorders for ZP2 Gene

MalaCards: The human disease database

(3) MalaCards diseases for ZP2 Gene - From: HGMD, Orphanet, DISEASES, and GeneCards

- elite association - COSMIC cancer census association via MalaCards
Search ZP2 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for ZP2

genes like me logo Genes that share disorders with ZP2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for ZP2 Gene

Publications for ZP2 Gene

  1. Conservation of mammalian secondary sperm receptor genes enables the promoter of the human gene to function in mouse oocytes. (PMID: 8385033) Liang LF … Dean J (Developmental biology 1993) 2 3 4 22 58
  2. A Recurrent Missense Mutation in ZP3 Causes Empty Follicle Syndrome and Female Infertility. (PMID: 28886344) Chen T … Chen ZJ (American journal of human genetics 2017) 3 4 58
  3. Detection of zona pellucida proteins during human folliculogenesis. (PMID: 18033806) Gook DA … Martic M (Human reproduction (Oxford, England) 2008) 3 22 58
  4. Biological effects of recombinant human zona pellucida proteins on sperm function. (PMID: 16407501) Caballero-Campo P … Gerton GL (Biology of reproduction 2006) 3 22 58
  5. Binding of recombinant human proacrosin/acrosin to zona pellucida (ZP) glycoproteins. I. Studies with recombinant human ZPA, ZPB, and ZPC. (PMID: 15950651) Furlong LI … Vazquez-Levin MH (Fertility and sterility 2005) 3 22 58

Products for ZP2 Gene

Sources for ZP2 Gene

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