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Aliases for SSFA2 Gene

Aliases for SSFA2 Gene

  • Sperm Specific Antigen 2 2 3 5
  • Cleavage Signal-1 Protein 2 3 4
  • Ki-Ras-Induced Actin-Interacting Protein 3 4
  • KRAS-Induced Actin-Interacting Protein 2 3
  • Sperm Associated Antigen 13 2 3
  • CS-1 3 4
  • KRAP 3 4
  • CS1 3 4
  • Sperm-Specific Antigen 2 3
  • KIAA1927 4
  • SPAG13 3

External Ids for SSFA2 Gene

Previous GeneCards Identifiers for SSFA2 Gene

  • GC02P180990
  • GC02P181439
  • GC02P182720
  • GC02P182959
  • GC02P182582
  • GC02P182464
  • GC02P174612

Summaries for SSFA2 Gene

GeneCards Summary for SSFA2 Gene

SSFA2 (Sperm Specific Antigen 2) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include signaling receptor binding and actin filament binding. An important paralog of this gene is CCDC129.

Gene Wiki entry for SSFA2 Gene

Additional gene information for SSFA2 Gene

No data available for Entrez Gene Summary , CIViC summary , UniProtKB/Swiss-Prot , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for SSFA2 Gene

Genomics for SSFA2 Gene

GeneHancer (GH) Regulatory Elements for SSFA2 Gene

Promoters and enhancers for SSFA2 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH02J181889 Promoter/Enhancer 2.5 EPDnew Ensembl ENCODE dbSUPER 650.7 +3.9 3913 11.7 HDGF PKNOX1 CLOCK FOXA2 ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 SSFA2 PPP1R1C ENSG00000260742
GH02J181841 Promoter 0.5 EPDnew 650.2 -50.4 -50424 0.1 SSFA2 ENSG00000260742 RNU6ATAC19P
GH02J181774 Enhancer 0.9 Ensembl ENCODE dbSUPER 11.2 -116.8 -116774 0.8 ATF4 JUND CEBPB CEBPG EP300 SSFA2 RNU6ATAC19P ENSG00000260742
GH02J181808 Enhancer 1.6 FANTOM5 Ensembl ENCODE dbSUPER 2.2 -79.6 -79608 7 PKNOX1 FOXA2 ZNF766 ATF7 FOS REST MEF2D GMEB1 SMARCA4 MYNN SSFA2 RNU6ATAC19P ENSG00000260742
GH02J181875 Enhancer 1.4 FANTOM5 Ensembl ENCODE dbSUPER 2.5 -15.0 -14966 2 MEIS2 KLF1 JUN MAX BRCA1 RFX5 ZNF335 FOSL1 POLR2A RCOR1 PPP1R1C SSFA2 ENSG00000260742 RNU6ATAC19P
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around SSFA2 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the SSFA2 gene promoter:
  • Nkx6-1
  • Nkx2-5
  • TBP
  • C/EBPalpha
  • ARP-1
  • E2F
  • E2F-1
  • POU2F1

Genomic Locations for SSFA2 Gene

Genomic Locations for SSFA2 Gene
39,023 bases
Plus strand
38,994 bases
Plus strand

Genomic View for SSFA2 Gene

Genes around SSFA2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
SSFA2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for SSFA2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SSFA2 Gene

Proteins for SSFA2 Gene

  • Protein details for SSFA2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Sperm-specific antigen 2
    Protein Accession:
    Secondary Accessions:
    • A8K6T0
    • Q68DA6
    • Q7Z7L2
    • Q8N1L3
    • Q8N263
    • Q8N7H2
    • Q8NEN5
    • Q96E36
    • Q96PW1

    Protein attributes for SSFA2 Gene

    1259 amino acids
    Molecular mass:
    138386 Da
    Quaternary structure:
    No Data Available
    • Sequence=AAB00773.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAH12947.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB67820.2; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAC05308.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for SSFA2 Gene


neXtProt entry for SSFA2 Gene

Post-translational modifications for SSFA2 Gene

  • A 33 kDa peptide corresponding to the C-terminus of this protein is found in the testis and seems to be cleaved into 2 peptides of 14 kDa and 18 kDa found on the surface of mature sperm cells. This sperm surface antigen may be involved in some step of early cleavage of the fertilized oocyte.
  • Ubiquitination at isoforms=2, 3212
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for SSFA2 Gene

Domains & Families for SSFA2 Gene

Gene Families for SSFA2 Gene

Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for SSFA2 Gene


Suggested Antigen Peptide Sequences for SSFA2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with SSFA2: view

No data available for UniProtKB/Swiss-Prot for SSFA2 Gene

Function for SSFA2 Gene

Molecular function for SSFA2 Gene

UniProtKB/Swiss-Prot Induction:
Up-regulated by activated KRAS.

Gene Ontology (GO) - Molecular Function for SSFA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005102 signaling receptor binding IEA --
GO:0051015 actin filament binding IDA 14673706
genes like me logo Genes that share ontologies with SSFA2: view
genes like me logo Genes that share phenotypes with SSFA2: view

Animal Models for SSFA2 Gene

MGI Knock Outs for SSFA2:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for SSFA2

Clone Products

No data available for Enzyme Numbers (IUBMB) , Phenotypes From GWAS Catalog , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for SSFA2 Gene

Localization for SSFA2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SSFA2 Gene

Cytoplasm. Note=Located near the plasma membrane. Associated with actin filaments. May also exist as a membrane-bound form with extracellular regions.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for SSFA2 gene
Compartment Confidence
plasma membrane 5
nucleus 5
cytosol 5
cytoskeleton 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (3)
  • Nucleus (2)
  • Plasma membrane (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for SSFA2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA --
GO:0005737 cytoplasm IEA --
GO:0005829 cytosol IDA --
GO:0005886 plasma membrane IEA,IDA --
genes like me logo Genes that share ontologies with SSFA2: view

Pathways & Interactions for SSFA2 Gene

SuperPathways for SSFA2 Gene

No Data Available

Gene Ontology (GO) - Biological Process for SSFA2 Gene


No data available for Pathways by source and SIGNOR curated interactions for SSFA2 Gene

Drugs & Compounds for SSFA2 Gene

No Compound Related Data Available

Transcripts for SSFA2 Gene

Unigene Clusters for SSFA2 Gene

Sperm specific antigen 2:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for SSFA2

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for SSFA2 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b · 12c · 12d ^ 13 ^ 14 ^ 15 ^ 16a · 16b · 16c ^ 17 ^ 18 ^
SP1: -
SP3: - -
SP4: - - - -
SP5: - -
SP6: - -
SP7: -

ExUns: 19a · 19b · 19c

Relevant External Links for SSFA2 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for SSFA2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for SSFA2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for SSFA2 Gene

This gene is overexpressed in Testis (14.2), Breast (11.7), Adrenal (10.1), and Cervix (7.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for SSFA2 Gene

NURSA nuclear receptor signaling pathways regulating expression of SSFA2 Gene:


SOURCE GeneReport for Unigene cluster for SSFA2 Gene:


mRNA Expression by UniProt/SwissProt for SSFA2 Gene:

Tissue specificity: Strongly expressed in pancreas and testis. Present in colon cancer cells (at protein level).

Evidence on tissue expression from TISSUES for SSFA2 Gene

  • Pancreas(4.8)
  • Nervous system(4.6)
  • Liver(4.3)
  • Intestine(2.2)
genes like me logo Genes that share expression patterns with SSFA2: view

No data available for mRNA differential expression in normal tissues , Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for SSFA2 Gene

Orthologs for SSFA2 Gene

This gene was present in the common ancestor of chordates.

Orthologs for SSFA2 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia SSFA2 34 33
  • 99.55 (n)
(Bos Taurus)
Mammalia SSFA2 34 33
  • 88.76 (n)
(Canis familiaris)
Mammalia SSFA2 34 33
  • 86.8 (n)
(Rattus norvegicus)
Mammalia Ssfa2 33
  • 82.39 (n)
(Mus musculus)
Mammalia Ssfa2 16 34 33
  • 81.43 (n)
(Monodelphis domestica)
Mammalia SSFA2 34
  • 75 (a)
(Ornithorhynchus anatinus)
Mammalia SSFA2 34
  • 70 (a)
(Gallus gallus)
Aves SSFA2 34 33
  • 68.36 (n)
(Anolis carolinensis)
Reptilia SSFA2 34
  • 57 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia ssfa2 33
  • 61.23 (n)
African clawed frog
(Xenopus laevis)
Amphibia Xl.18482 33
(Danio rerio)
Actinopterygii ssfa2 34 33
  • 50.13 (n)
Species where no ortholog for SSFA2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for SSFA2 Gene

Gene Tree for SSFA2 (if available)
Gene Tree for SSFA2 (if available)
Evolutionary constrained regions (ECRs) for SSFA2: view image

Paralogs for SSFA2 Gene

Paralogs for SSFA2 Gene

genes like me logo Genes that share paralogs with SSFA2: view

Variants for SSFA2 Gene

Sequence variations from dbSNP and Humsavar for SSFA2 Gene

SNP ID Clin Chr 02 pos Variation AA Info Type
rs1000100482 -- 181,900,724(+) G/A coding_sequence_variant, genic_downstream_transcript_variant, genic_upstream_transcript_variant, missense_variant, non_coding_transcript_variant
rs1000171421 -- 181,927,464(+) T/G genic_downstream_transcript_variant, intron_variant
rs1000267058 -- 181,901,249(+) C/T genic_downstream_transcript_variant, genic_upstream_transcript_variant, intron_variant
rs1000308719 -- 181,918,150(+) C/G/T downstream_transcript_variant, genic_downstream_transcript_variant, intron_variant
rs1000413491 -- 181,892,855(+) C/T 3_prime_UTR_variant, genic_upstream_transcript_variant, intron_variant

Structural Variations from Database of Genomic Variants (DGV) for SSFA2 Gene

Variant ID Type Subtype PubMed ID
esv3593549 CNV loss 21293372
esv3593550 CNV gain 21293372
esv3593551 CNV gain 21293372
esv3593554 CNV loss 21293372
nsv834478 CNV gain 17160897
nsv834479 CNV gain 17160897

Variation tolerance for SSFA2 Gene

Residual Variation Intolerance Score: 45.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 8.73; 86.59% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for SSFA2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SSFA2 Gene

Disorders for SSFA2 Gene

Additional Disease Information for SSFA2

No disorders were found for SSFA2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for SSFA2 Gene

Publications for SSFA2 Gene

  1. Human cleavage signal-1 protein; cDNA cloning, transcription and immunological analysis. (PMID: 1555770) Javed AA … Naz RK (Gene 1992) 2 3 4 22 58
  2. Tespa1 protein is phosphorylated in response to store-operated calcium entry. (PMID: 23541577) Fujimoto T … Shirasawa S (Biochemical and biophysical research communications 2013) 2 3 58
  3. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PMID: 17081983) Olsen JV … Mann M (Cell 2006) 3 4 58
  4. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PMID: 15815621) Hillier LW … Wilson RK (Nature 2005) 3 4 58
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4 58

Products for SSFA2 Gene

Sources for SSFA2 Gene

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