The protein encoded by this gene enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. The encoded protein may function by altering local chromatin structure. [provided by RefSeq, Jul 2008] See more...

Aliases for ZNF335 Gene

Aliases for ZNF335 Gene

  • Zinc Finger Protein 335 2 3 4 5
  • NRC-Interacting Factor 1 2 3 4
  • NIF-1 2 3 4
  • Zinc-Finger/Leucine-Zipper Co-Transducer NIF1 3
  • BA465L10.2 2
  • MCPH10 3
  • ZNF335 5
  • NIF1 3
  • NIF2 3

External Ids for ZNF335 Gene

Previous GeneCards Identifiers for ZNF335 Gene

  • GC20M044305
  • GC20M045215
  • GC20M045262
  • GC20M044010
  • GC20M044577
  • GC20M041318

Summaries for ZNF335 Gene

Entrez Gene Summary for ZNF335 Gene

  • The protein encoded by this gene enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. The encoded protein may function by altering local chromatin structure. [provided by RefSeq, Jul 2008]

GeneCards Summary for ZNF335 Gene

ZNF335 (Zinc Finger Protein 335) is a Protein Coding gene. Diseases associated with ZNF335 include Microcephaly 10, Primary, Autosomal Recessive and Microcephaly 16, Primary, Autosomal Recessive. Among its related pathways are Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 and Developmental Biology. Gene Ontology (GO) annotations related to this gene include transcription regulatory region DNA binding and RNA polymerase II core promoter sequence-specific DNA binding. An important paralog of this gene is ZFP64.

UniProtKB/Swiss-Prot Summary for ZNF335 Gene

  • Component or associated component of some histone methyltransferase complexes may regulate transcription through recruitment of those complexes on gene promoters (PubMed:19131338, PubMed:23178126). Enhances ligand-dependent transcriptional activation by nuclear hormone receptors (PubMed:12215545, PubMed:18180299, PubMed:19131338). Plays an important role in neural progenitor cell proliferation and self-renewal through the regulation of specific genes involved brain development, including REST (PubMed:23178126). Also controls the expression of genes involved in somatic development and regulates, for instance, lymphoblast proliferation (PubMed:23178126).

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for ZNF335 Gene

Genomics for ZNF335 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for ZNF335 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around ZNF335 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for ZNF335

Top Transcription factor binding sites by QIAGEN in the ZNF335 gene promoter:
  • AP-1
  • ATF-2
  • c-Jun
  • CREB
  • deltaCREB
  • NF-kappaB
  • NF-kappaB1
  • NF-kappaB2
  • RelA

Genomic Locations for ZNF335 Gene

Genomic Locations for ZNF335 Gene
chr20:45,948,653-45,972,910
(GRCh38/hg38)
Size:
24,258 bases
Orientation:
Minus strand
chr20:44,577,292-44,600,833
(GRCh37/hg19)
Size:
23,542 bases
Orientation:
Minus strand

Genomic View for ZNF335 Gene

Genes around ZNF335 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
ZNF335 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for ZNF335 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for ZNF335 Gene

Proteins for ZNF335 Gene

  • Protein details for ZNF335 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H4Z2-ZN335_HUMAN
    Recommended name:
    Zinc finger protein 335
    Protein Accession:
    Q9H4Z2
    Secondary Accessions:
    • B4DLG7
    • Q548D0
    • Q9H684

    Protein attributes for ZNF335 Gene

    Size:
    1342 amino acids
    Molecular mass:
    144893 Da
    Quaternary structure:
    • Interacts with NCOA6; may enhance ligand-dependent transcriptional activation by nuclear hormone receptors (PubMed:12215545). Interacts with CNOT6 (PubMed:18180299). Interacts with CNOT9; the interaction is direct (PubMed:18180299). Component of a nuclear receptor-mediated transcription complex composed of at least ZNF335, CCAR2 and EMSY; the complex stimulates the transcription of nuclear receptor target genes such as SOX9 and HOXA1 (PubMed:19131338). Within the complex interacts with EMSY and interacts (via C-terminus) with CCAR2 (PubMed:19131338). Interacts with members of histone H3'Lys4'(H3K4) methyltransferase complexes ASCL2, CXXC1, KMT2A/MLL1, RBBP5, SETD1A and WDR5 (PubMed:23178126). Component of a histone methylation complex composed of at least ZNF335, RBBP5, ASH2L and WDR5; the complex may have histone H3-specific methyltransferase activity, however does not have specificity for 'Lys-4' of histone H3 (PubMed:19131338). Interacts with RBBP5 and WDR5 (PubMed:19131338). Interacts with ASHL2 (PubMed:19131338). Components of this complex may associate with components of the ZNF335-CCAR2-EMSY nuclear receptor-mediated transcription complex to form a complex at least composed of ZNF335, HCFC1, CCAR2, EMSY, MKI67, RBBP5, ASH2L and WDR5 (PubMed:19131338). Within this complex also interacts with HCFC1 and MKI67 (PubMed:19131338).
    SequenceCaution:
    • Sequence=BAB15379.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for ZNF335 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for ZNF335 Gene

Post-translational modifications for ZNF335 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for ZNF335 Gene

No data available for DME Specific Peptides for ZNF335 Gene

Domains & Families for ZNF335 Gene

Gene Families for ZNF335 Gene

HGNC:
Human Protein Atlas (HPA):
  • Disease related genes
  • Predicted intracellular proteins
  • Transcription factors

Protein Domains for ZNF335 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for ZNF335 Gene

GenScript: Design optimal peptide antigens:
  • ZNF335 protein (Q8IW09_HUMAN)
  • NRC-interacting factor 1 (ZN335_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9H4Z2

UniProtKB/Swiss-Prot:

ZN335_HUMAN :
  • Belongs to the krueppel C2H2-type zinc-finger protein family.
Family:
  • Belongs to the krueppel C2H2-type zinc-finger protein family.
genes like me logo Genes that share domains with ZNF335: view

Function for ZNF335 Gene

Molecular function for ZNF335 Gene

UniProtKB/Swiss-Prot Function:
Component or associated component of some histone methyltransferase complexes may regulate transcription through recruitment of those complexes on gene promoters (PubMed:19131338, PubMed:23178126). Enhances ligand-dependent transcriptional activation by nuclear hormone receptors (PubMed:12215545, PubMed:18180299, PubMed:19131338). Plays an important role in neural progenitor cell proliferation and self-renewal through the regulation of specific genes involved brain development, including REST (PubMed:23178126). Also controls the expression of genes involved in somatic development and regulates, for instance, lymphoblast proliferation (PubMed:23178126).

Phenotypes From GWAS Catalog for ZNF335 Gene

Gene Ontology (GO) - Molecular Function for ZNF335 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000976 transcription regulatory region sequence-specific DNA binding ISS --
GO:0000978 RNA polymerase II proximal promoter sequence-specific DNA binding IEA,IBA 21873635
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific ISM 19274049
GO:0003676 nucleic acid binding IEA --
GO:0003677 DNA binding IEA --
genes like me logo Genes that share ontologies with ZNF335: view
genes like me logo Genes that share phenotypes with ZNF335: view

Human Phenotype Ontology for ZNF335 Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for ZNF335 Gene

MGI Knock Outs for ZNF335:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ZNF335

No data available for Enzyme Numbers (IUBMB) , miRNA , Transcription Factor Targets and HOMER Transcription for ZNF335 Gene

Localization for ZNF335 Gene

Subcellular locations from UniProtKB/Swiss-Prot for ZNF335 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for ZNF335 gene
Compartment Confidence
nucleus 5
cytosol 2
cytoskeleton 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for ZNF335 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IBA,ISS --
GO:0005654 nucleoplasm IDA --
GO:0035097 colocalizes_with histone methyltransferase complex IEA,IDA 23178126
genes like me logo Genes that share ontologies with ZNF335: view

Pathways & Interactions for ZNF335 Gene

genes like me logo Genes that share pathways with ZNF335: view

Gene Ontology (GO) - Biological Process for ZNF335 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001701 in utero embryonic development IEA --
GO:0002052 positive regulation of neuroblast proliferation IEA,ISS --
GO:0006357 regulation of transcription by RNA polymerase II IEA --
GO:0007275 multicellular organism development IEA --
GO:0007420 brain development IEA,IMP 23178126
genes like me logo Genes that share ontologies with ZNF335: view

No data available for SIGNOR curated interactions for ZNF335 Gene

Drugs & Compounds for ZNF335 Gene

No Compound Related Data Available

Transcripts for ZNF335 Gene

mRNA/cDNA for ZNF335 Gene

1 REFSEQ mRNAs :
11 NCBI additional mRNA sequence :
4 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for ZNF335

Alternative Splicing Database (ASD) splice patterns (SP) for ZNF335 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3a · 3b · 3c ^ 4a · 4b · 4c · 4d ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9a · 9b · 9c ^ 10a · 10b · 10c · 10d ^ 11a · 11b ^
SP1:
SP2:
SP3:
SP4:
SP5:
SP6: - - -
SP7:
SP8:
SP9:
SP10:
SP11: - - - -
SP12: - - - -
SP13: - -
SP14: - -
SP15:
SP16:
SP17:
SP18: -
SP19:
SP20:
SP21: - -
SP22:

ExUns: 12a · 12b ^ 13 ^ 14 ^ 15a · 15b ^ 16a · 16b · 16c · 16d ^ 17a · 17b ^ 18a · 18b ^ 19a · 19b ^ 20 ^ 21a · 21b · 21c · 21d ^ 22a · 22b ^ 23 ^ 24 ^ 25 ^
SP1: - - -
SP2: -
SP3: - -
SP4:
SP5: -
SP6:
SP7:
SP8:
SP9:
SP10: -
SP11:
SP12:
SP13:
SP14:
SP15:
SP16: -
SP17:
SP18:
SP19:
SP20:
SP21:
SP22:

ExUns: 26 ^ 27a · 27b ^ 28 ^ 29a · 29b
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:
SP15:
SP16:
SP17:
SP18:
SP19:
SP20:
SP21:
SP22:

Relevant External Links for ZNF335 Gene

GeneLoc Exon Structure for
ZNF335

Expression for ZNF335 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for ZNF335 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for ZNF335 Gene

This gene is overexpressed in Blymphocyte (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for ZNF335 Gene



Protein tissue co-expression partners for ZNF335 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for ZNF335

SOURCE GeneReport for Unigene cluster for ZNF335 Gene:

Hs.174193

mRNA Expression by UniProt/SwissProt for ZNF335 Gene:

Q9H4Z2-ZN335_HUMAN
Tissue specificity: Ubiquitously expressed.

Evidence on tissue expression from TISSUES for ZNF335 Gene

  • Kidney(4.1)
  • Liver(4.1)
  • Nervous system(2.7)

Phenotype-based relationships between genes and organs from Gene ORGANizer for ZNF335 Gene

Germ Layers:
  • ectoderm
  • endoderm
  • mesoderm
Systems:
  • nervous
  • respiratory
  • skeletal muscle
  • skeleton
Regions:
Head and neck:
  • brain
  • cerebellum
  • chin
  • eye
  • face
  • forehead
  • head
  • jaw
  • mandible
  • maxilla
  • mouth
  • nose
  • pharynx
  • skull
Limb:
  • ankle
  • digit
  • elbow
  • finger
  • foot
  • hand
  • hip
  • knee
  • lower limb
  • shoulder
  • toe
  • upper limb
  • wrist
General:
  • spinal cord
genes like me logo Genes that share expression patterns with ZNF335: view

Primer Products

No data available for mRNA differential expression in normal tissues for ZNF335 Gene

Orthologs for ZNF335 Gene

This gene was present in the common ancestor of chordates.

Orthologs for ZNF335 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia ZNF335 30 31
  • 98.35 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia ZNF335 30 31
  • 89.16 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia ZNF335 30 31
  • 87.63 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Zfp335 30
  • 84.95 (n)
Mouse
(Mus musculus)
Mammalia Zfp335 30 17 31
  • 84.27 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia ZNF335 31
  • 79 (a)
OneToOne
Chicken
(Gallus gallus)
Aves ZNF335 30 31
  • 70.65 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia -- 31
  • 81 (a)
OneToMany
-- 31
  • 47 (a)
OneToMany
African clawed frog
(Xenopus laevis)
Amphibia Xl.27543 30
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia Str.1147 30
Zebrafish
(Danio rerio)
Actinopterygii LOC100148891 30
  • 61.45 (n)
ZNF335 31
  • 53 (a)
OneToOne
Species where no ortholog for ZNF335 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for ZNF335 Gene

ENSEMBL:
Gene Tree for ZNF335 (if available)
TreeFam:
Gene Tree for ZNF335 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for ZNF335: view image

Paralogs for ZNF335 Gene

Paralogs for ZNF335 Gene

(7) SIMAP similar genes for ZNF335 Gene using alignment to 2 proteins:

  • ZN335_HUMAN
  • Q8IW09_HUMAN
genes like me logo Genes that share paralogs with ZNF335: view

Variants for ZNF335 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for ZNF335 Gene

SNP ID Clinical significance and condition Chr 20 pos Variation AA Info Type
638420 Uncertain Significance: Primary autosomal recessive microcephaly 10 45,969,658(-) G/A MISSENSE_VARIANT
709516 Benign: not provided 45,971,308(-) C/A MISSENSE_VARIANT
712868 Benign: not provided 45,952,350(-) T/C MISSENSE_VARIANT
716509 Benign: not provided 45,950,068(-) A/G SYNONYMOUS_VARIANT
718966 Likely Benign: not provided 45,969,488(-) C/T SYNONYMOUS_VARIANT

Additional dbSNP identifiers (rs#s) for ZNF335 Gene

Structural Variations from Database of Genomic Variants (DGV) for ZNF335 Gene

Variant ID Type Subtype PubMed ID
esv1000107 CNV deletion 20482838
nsv180559 CNV deletion 16902084
nsv517296 CNV loss 19592680
nsv833991 CNV loss 17160897

Variation tolerance for ZNF335 Gene

Residual Variation Intolerance Score: 2.41% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.56; 65.04% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for ZNF335 Gene

Human Gene Mutation Database (HGMD)
ZNF335
SNPedia medical, phenotypic, and genealogical associations of SNPs for
ZNF335

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for ZNF335 Gene

Disorders for ZNF335 Gene

MalaCards: The human disease database

(22) MalaCards diseases for ZNF335 Gene - From: UniProtKB/Swiss-Prot, OMIM, ClinVar, GTR, Orphanet, DISEASES, and GeneCards

UniProtKB/Swiss-Prot

ZN335_HUMAN
  • Microcephaly 10, primary, autosomal recessive (MCPH10) [MIM:615095]: A form of microcephaly, a disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. MCPH10 is characterized by extremely small head size and death usually by 1 year of age. Neuropathologic examination shows severe loss of neurons as well as neuronal loss of polarity and abnormal dendritic maturation. {ECO:0000269 PubMed:23178126}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Additional Disease Information for ZNF335

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with ZNF335: view

No data available for Genatlas for ZNF335 Gene

Publications for ZNF335 Gene

  1. Identification and characterization of a novel nuclear protein complex involved in nuclear hormone receptor-mediated gene regulation. (PMID: 19131338) Garapaty S … Samuels HH (The Journal of biological chemistry 2009) 2 3 4 23
  2. NRC-interacting factor 1 is a novel cotransducer that interacts with and regulates the activity of the nuclear hormone receptor coactivator NRC. (PMID: 12215545) Mahajan MA … Samuels HH (Molecular and cellular biology 2002) 2 3 4 23
  3. Components of the CCR4-NOT complex function as nuclear hormone receptor coactivators via association with the NRC-interacting Factor NIF-1. (PMID: 18180299) Garapaty S … Samuels HH (The Journal of biological chemistry 2008) 3 4 23
  4. Microcephaly gene links trithorax and REST/NRSF to control neural stem cell proliferation and differentiation. (PMID: 23178126) Yang YJ … Walsh CA (Cell 2012) 3 4
  5. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 41

Products for ZNF335 Gene

  • Signalway ELISA kits for ZNF335
  • Signalway Proteins for ZNF335

Sources for ZNF335 Gene