The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing facto... See more...

Aliases for WTAP Gene

Aliases for WTAP Gene

  • WT1 Associated Protein 2 3 5
  • Wilms Tumor 1-Associating Protein 3 4
  • Pre-MRNA-Splicing Regulator WTAP 3 4
  • Wilms Tumor 1 Associated Protein 2 3
  • Female-Lethal(2)D Homolog 3 4
  • HFL(2)D 3 4
  • Putative Pre-MRNA Splicing Regulator Female-Lethal(2D) 3
  • Wilms' Tumour 1-Associating Protein 3
  • WT1-Associated Protein 4
  • PNAS-132 3
  • KIAA0105 4
  • Mum2 3

External Ids for WTAP Gene

Previous GeneCards Identifiers for WTAP Gene

  • GC06P159587
  • GC06P160021
  • GC06P159981
  • GC06P160057
  • GC06P160118
  • GC06P157617
  • GC06P160146

Summaries for WTAP Gene

Entrez Gene Summary for WTAP Gene

  • The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012]

GeneCards Summary for WTAP Gene

WTAP (WT1 Associated Protein) is a Protein Coding gene. Diseases associated with WTAP include Wilms Tumor 1 and Wilms Tumor 5. Among its related pathways are Gene Expression and Chromatin Regulation / Acetylation.

UniProtKB/Swiss-Prot Summary for WTAP Gene

  • Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).

Gene Wiki entry for WTAP Gene

Additional gene information for WTAP Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for WTAP Gene

Genomics for WTAP Gene

GeneHancer (GH) Regulatory Elements for WTAP Gene

Promoters and enhancers for WTAP Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around WTAP on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the WTAP gene promoter:
  • GATA-2
  • HFH-1
  • Ik-3
  • MyoD
  • SEF-1 (1)

Genomic Locations for WTAP Gene

Genomic Locations for WTAP Gene
chr6:159,725,585-159,756,319
(GRCh38/hg38)
Size:
30,735 bases
Orientation:
Plus strand
chr6:160,146,617-160,177,351
(GRCh37/hg19)
Size:
30,735 bases
Orientation:
Plus strand

Genomic View for WTAP Gene

Genes around WTAP on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
WTAP Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for WTAP Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for WTAP Gene

Proteins for WTAP Gene

  • Protein details for WTAP Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q15007-FL2D_HUMAN
    Recommended name:
    Pre-mRNA-splicing regulator WTAP
    Protein Accession:
    Q15007
    Secondary Accessions:
    • Q5TCL8
    • Q5TCL9
    • Q96T28
    • Q9BYJ7
    • Q9H4E2

    Protein attributes for WTAP Gene

    Size:
    396 amino acids
    Molecular mass:
    44244 Da
    Quaternary structure:
    • Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM (PubMed:24407421, PubMed:24981863, PubMed:24316715, PubMed:29507755, PubMed:29506078, PubMed:29348140). The MAC subcomplex is composed of METTL3 and METTL14 (PubMed:29507755). The MACOM subcomplex is composed of WTAP, ZC3H13, CBLL1/HAKAI, VIRMA, and, in some cases of RBM15 (RBM15 or RBM15B) (PubMed:29507755). Interacts with WT1 (PubMed:11001926, PubMed:17095724). Also component of a MACOM-like complex, named WTAP complex, composed of WTAP, ZC3H13, CBLL1, VIRMA, RBM15, BCLAF1 and THRAP3 (PubMed:24100041).

    Alternative splice isoforms for WTAP Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for WTAP Gene

Post-translational modifications for WTAP Gene

  • Ubiquitination at Lys83 and Lys98
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for WTAP Gene

Domains & Families for WTAP Gene

Gene Families for WTAP Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for WTAP Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for WTAP Gene

GenScript: Design optimal peptide antigens:
  • Wilms tumor 1-associating protein (FL2D_HUMAN)
  • cDNA FLJ45925 fis, clone PLACE6016030, highly similar to Wilms' tumor 1-associating protein (Q5TCL9_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q15007

UniProtKB/Swiss-Prot:

FL2D_HUMAN :
  • Belongs to the fl(2)d family.
Family:
  • Belongs to the fl(2)d family.
genes like me logo Genes that share domains with WTAP: view

Function for WTAP Gene

Molecular function for WTAP Gene

UniProtKB/Swiss-Prot Function:
Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).
UniProtKB/Swiss-Prot Induction:
In smooth muscle cells, up-regulated after serum withdrawal, when cells become mature and non proliferative.

Phenotypes From GWAS Catalog for WTAP Gene

Gene Ontology (GO) - Molecular Function for WTAP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 16189514
genes like me logo Genes that share ontologies with WTAP: view
genes like me logo Genes that share phenotypes with WTAP: view

Animal Model Products

  • Taconic Biosciences Mouse Models for WTAP

CRISPR Products

Clone Products

  • Addgene plasmids for WTAP

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for WTAP Gene

Localization for WTAP Gene

Subcellular locations from UniProtKB/Swiss-Prot for WTAP Gene

Nucleus speckle. Nucleus, nucleoplasm. Cytoplasm. Note=Mainly nuclear with some fraction located in the cytoplasm. ZC3H13 is required to anchor component of the MACOM subcomplex, such as VIRMA, in the nucleus. {ECO:0000250 UniProtKB:Q9ER69}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for WTAP gene
Compartment Confidence
nucleus 5
cytosol 2

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nuclear speckles (4)
  • Nucleoplasm (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for WTAP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA,IEA 10942595
GO:0005654 nucleoplasm TAS,IDA --
GO:0005737 cytoplasm ISS --
GO:0016607 nuclear speck IEA,IDA 24100041
GO:0031965 nuclear membrane IDA 10942595
genes like me logo Genes that share ontologies with WTAP: view

Pathways & Interactions for WTAP Gene

genes like me logo Genes that share pathways with WTAP: view

Pathways by source for WTAP Gene

1 Cell Signaling Technology pathway for WTAP Gene

Gene Ontology (GO) - Biological Process for WTAP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000381 regulation of alternative mRNA splicing, via spliceosome IMP 24407421
GO:0006397 mRNA processing IEA --
GO:0007049 cell cycle IEA --
GO:0007275 multicellular organism development IEA --
GO:0008380 RNA splicing IEA --
genes like me logo Genes that share ontologies with WTAP: view

No data available for SIGNOR curated interactions for WTAP Gene

Drugs & Compounds for WTAP Gene

No Compound Related Data Available

Transcripts for WTAP Gene

CRISPR Products

Clone Products

  • Addgene plasmids for WTAP

Alternative Splicing Database (ASD) splice patterns (SP) for WTAP Gene

No ASD Table

Relevant External Links for WTAP Gene

GeneLoc Exon Structure for
WTAP
ECgene alternative splicing isoforms for
WTAP

Expression for WTAP Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for WTAP Gene

Protein differential expression in normal tissues from HIPED for WTAP Gene

This gene is overexpressed in Peripheral blood mononuclear cells (17.7) and Lymph node (7.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for WTAP Gene



Protein tissue co-expression partners for WTAP Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of WTAP Gene:

WTAP

SOURCE GeneReport for Unigene cluster for WTAP Gene:

Hs.446091

mRNA Expression by UniProt/SwissProt for WTAP Gene:

Q15007-FL2D_HUMAN
Tissue specificity: Ubiquitously expressed.

Evidence on tissue expression from TISSUES for WTAP Gene

  • Nervous system(4.7)
  • Lung(4.6)
  • Bone marrow(4.4)
  • Stomach(3.5)
  • Spleen(2.2)
  • Heart(2.1)
  • Liver(2.1)
  • Muscle(2.1)
  • Intestine(2)
genes like me logo Genes that share expression patterns with WTAP: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for WTAP Gene

Orthologs for WTAP Gene

This gene was present in the common ancestor of animals.

Orthologs for WTAP Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia WTAP 33 32
  • 99.75 (n)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 33
  • 98 (a)
OneToMany
-- 33
  • 85 (a)
OneToMany
-- 33
  • 85 (a)
OneToMany
dog
(Canis familiaris)
Mammalia WTAP 32
  • 95.45 (n)
-- 33
  • 95 (a)
OneToMany
-- 33
  • 94 (a)
OneToMany
-- 33
  • 92 (a)
OneToMany
-- 33
  • 91 (a)
OneToMany
cow
(Bos Taurus)
Mammalia WTAP 33 32
  • 94.28 (n)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia WTAP 33
  • 94 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Wtap 32
  • 93.92 (n)
mouse
(Mus musculus)
Mammalia Wtap 17 33 32
  • 93.77 (n)
chicken
(Gallus gallus)
Aves WTAP 33 32
  • 87.71 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia WTAP 33
  • 90 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia wtap 32
  • 78.57 (n)
zebrafish
(Danio rerio)
Actinopterygii wtap 33 32
  • 72.42 (n)
OneToOne
wufi29a09 32
fruit fly
(Drosophila melanogaster)
Insecta fl(2)d 33 34
  • 24 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea CSA.7034 33
  • 57 (a)
OneToOne
Species where no ortholog for WTAP was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for WTAP Gene

ENSEMBL:
Gene Tree for WTAP (if available)
TreeFam:
Gene Tree for WTAP (if available)
Aminode:
Evolutionary constrained regions (ECRs) for WTAP: view image

Paralogs for WTAP Gene

(1) SIMAP similar genes for WTAP Gene using alignment to 1 proteins:

  • FL2D_HUMAN

Pseudogenes.org Pseudogenes for WTAP Gene

genes like me logo Genes that share paralogs with WTAP: view

No data available for Paralogs for WTAP Gene

Variants for WTAP Gene

Sequence variations from dbSNP and Humsavar for WTAP Gene

SNP ID Clin Chr 06 pos Variation AA Info Type
rs1000024361 -- 159,731,391(+) C/A genic_upstream_transcript_variant, intron_variant, upstream_transcript_variant
rs1000045877 -- 159,756,818(+) G/A/T downstream_transcript_variant
rs1000138692 -- 159,741,814(+) C/A intron_variant
rs1000196404 -- 159,750,763(+) C/G/T genic_downstream_transcript_variant, intron_variant
rs1000389129 -- 159,733,393(+) G/A genic_upstream_transcript_variant, intron_variant

Structural Variations from Database of Genomic Variants (DGV) for WTAP Gene

Variant ID Type Subtype PubMed ID
esv2759483 CNV gain 17122850
nsv949879 CNV duplication 24416366
nsv981201 CNV duplication 23825009

Variation tolerance for WTAP Gene

Residual Variation Intolerance Score: 23.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.64; 13.69% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for WTAP Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
WTAP

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for WTAP Gene

Disorders for WTAP Gene

MalaCards: The human disease database

(2) MalaCards diseases for WTAP Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
wilms tumor 1
  • wt1
wilms tumor 5
  • nephroblastoma
- elite association - COSMIC cancer census association via MalaCards
Search WTAP in MalaCards View complete list of genes associated with diseases

Additional Disease Information for WTAP

genes like me logo Genes that share disorders with WTAP: view

No data available for UniProtKB/Swiss-Prot and Genatlas for WTAP Gene

Publications for WTAP Gene

  1. Identification of WTAP, a novel Wilms' tumour 1-associating protein. (PMID: 11001926) Little NA … Davies RC (Human molecular genetics 2000) 2 3 4 23 56
  2. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase. (PMID: 24407421) Ping XL … Yang YG (Cell research 2014) 2 3 4 56
  3. Wilms' tumor 1-associating protein regulates G2/M transition through stabilization of cyclin A2 mRNA. (PMID: 17088532) Horiuchi K … Kodama T (Proceedings of the National Academy of Sciences of the United States of America 2006) 3 4 23 56
  4. Wilms' tumor 1-associating protein regulates the proliferation of vascular smooth muscle cells. (PMID: 17095724) Small TW … Pickering JG (Circulation research 2006) 3 4 23 56
  5. Biochemical function of female-lethal (2)D/Wilms' tumor suppressor-1-associated proteins in alternative pre-mRNA splicing. (PMID: 12444081) Ortega A … Valcárcel J (The Journal of biological chemistry 2003) 3 4 23 56

Products for WTAP Gene

Sources for WTAP Gene