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Aliases for WFDC2 Gene

Aliases for WFDC2 Gene

  • WAP Four-Disulfide Core Domain 2 2 3 5
  • Major Epididymis-Specific Protein E4 3 4
  • Putative Protease Inhibitor WAP5 3 4
  • Epididymal Secretory Protein E4 3 4
  • Epididymal Protein 4 2 3
  • WAP5 3 4
  • HE4 3 4
  • Epididymis-Specific, Whey-Acidic Protein Type, Four-Disulfide Core 3
  • WAP Four-Disulfide Core Domain Protein 2 3
  • WAP Domain Containing Protein HE4-V4 3
  • DJ461P17.6 3
  • EDDM4 3

External Ids for WFDC2 Gene

Previous GeneCards Identifiers for WFDC2 Gene

  • GC20P043826
  • GC20P044736
  • GC20P044783
  • GC20P043532
  • GC20P044098
  • GC20P040839

Summaries for WFDC2 Gene

Entrez Gene Summary for WFDC2 Gene

  • This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

GeneCards Summary for WFDC2 Gene

WFDC2 (WAP Four-Disulfide Core Domain 2) is a Protein Coding gene. Diseases associated with WFDC2 include Ovarian Cancer. Gene Ontology (GO) annotations related to this gene include serine-type endopeptidase inhibitor activity and cysteine-type endopeptidase inhibitor activity.

UniProtKB/Swiss-Prot for WFDC2 Gene

  • Broad range protease inhibitor.

Gene Wiki entry for WFDC2 Gene

Additional gene information for WFDC2 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for WFDC2 Gene

Genomics for WFDC2 Gene

GeneHancer (GH) Regulatory Elements for WFDC2 Gene

Promoters and enhancers for WFDC2 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH20I045469 Promoter/Enhancer 1.4 EPDnew Ensembl ENCODE 550.3 +21.7 21676 2.1 CTCF KLF1 MZF1 ZIC2 RAD21 RELA ZNF335 ZFHX2 POLR2A ZBTB48 WFDC2 PIGT ENSG00000237464
GH20I045450 Enhancer 0.9 ENCODE dbSUPER 550.8 +1.9 1948 0.3 HDAC1 CTCF TEAD4 MAX CEBPG RAD21 TEAD3 CTBP1 ZNF143 SMC3 WFDC2 GC20P045440 PIGT ENSG00000237464
GH20I045449 Enhancer 0.8 ENCODE dbSUPER 550.8 +1.2 1229 0.9 CTCF TRIM22 ATF2 ZFP69B HNF1A CTBP1 ZNF692 ZNF629 PRDM10 ZNF24 ENSG00000277022 WFDC2 GC20P045440 PIGT ENSG00000237464
GH20I045448 Enhancer 0.6 ENCODE dbSUPER 550.8 -0.1 -62 0.4 GATA3 FOXA1 ENSG00000277022 WFDC2 GC20P045440 PIGT
GH20I045452 Enhancer 0.3 Ensembl 0.8 +4.4 4353 0.4 SREBF1 ENSG00000237464 WFDC2 GC20P045440 PIGT
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around WFDC2 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the WFDC2 gene promoter:

Genomic Locations for WFDC2 Gene

Genomic Locations for WFDC2 Gene
32,885 bases
Plus strand

Genomic View for WFDC2 Gene

Genes around WFDC2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
WFDC2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for WFDC2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for WFDC2 Gene

Proteins for WFDC2 Gene

  • Protein details for WFDC2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    WAP four-disulfide core domain protein 2
    Protein Accession:
    Secondary Accessions:
    • A2A2A5
    • A2A2A6
    • A6PVD5
    • Q6IB27
    • Q8WXV9
    • Q8WXW0
    • Q8WXW1
    • Q8WXW2
    • Q96KJ1

    Protein attributes for WFDC2 Gene

    124 amino acids
    Molecular mass:
    12993 Da
    Quaternary structure:
    • Homotrimer; disulfide-linked.

    Alternative splice isoforms for WFDC2 Gene


neXtProt entry for WFDC2 Gene

Post-translational modifications for WFDC2 Gene

Other Protein References for WFDC2 Gene

No data available for DME Specific Peptides for WFDC2 Gene

Domains & Families for WFDC2 Gene

Gene Families for WFDC2 Gene

Human Protein Atlas (HPA):
  • Cancer-related genes
  • Predicted intracellular proteins
  • Predicted secreted proteins

Protein Domains for WFDC2 Gene


Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with WFDC2: view

No data available for UniProtKB/Swiss-Prot for WFDC2 Gene

Function for WFDC2 Gene

Molecular function for WFDC2 Gene

UniProtKB/Swiss-Prot Function:
Broad range protease inhibitor.

Gene Ontology (GO) - Molecular Function for WFDC2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004866 endopeptidase inhibitor activity TAS 1686187
GO:0004867 serine-type endopeptidase inhibitor activity IDA 23139753
GO:0004869 cysteine-type endopeptidase inhibitor activity IEA --
GO:0019828 aspartic-type endopeptidase inhibitor activity IDA 23139753
GO:0030414 peptidase inhibitor activity IEA --
genes like me logo Genes that share ontologies with WFDC2: view

Phenotypes for WFDC2 Gene

GenomeRNAi human phenotypes for WFDC2:
genes like me logo Genes that share phenotypes with WFDC2: view

Animal Model Products

Clone Products

  • Addgene plasmids for WFDC2

No data available for Enzyme Numbers (IUBMB) , Phenotypes From GWAS Catalog , Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for WFDC2 Gene

Localization for WFDC2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for WFDC2 Gene


Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for WFDC2 gene
Compartment Confidence
extracellular 5
cytosol 2
nucleus 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for WFDC2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region IEA --
GO:0005615 extracellular space TAS 1686187
GO:0070062 extracellular exosome IDA,HDA 23376485
genes like me logo Genes that share ontologies with WFDC2: view

Pathways & Interactions for WFDC2 Gene

SuperPathways for WFDC2 Gene

No Data Available

Interacting Proteins for WFDC2 Gene

Gene Ontology (GO) - Biological Process for WFDC2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis TAS 1686187
GO:0007283 spermatogenesis TAS 1686187
GO:0010466 negative regulation of peptidase activity IEA --
GO:0010951 negative regulation of endopeptidase activity IEA --
genes like me logo Genes that share ontologies with WFDC2: view

No data available for Pathways by source and SIGNOR curated interactions for WFDC2 Gene

Drugs & Compounds for WFDC2 Gene

(1) Drugs for WFDC2 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials

(1) Additional Compounds for WFDC2 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with WFDC2: view

Transcripts for WFDC2 Gene

Unigene Clusters for WFDC2 Gene

WAP four-disulfide core domain 2:
Representative Sequences:

Clone Products

  • Addgene plasmids for WFDC2

Alternative Splicing Database (ASD) splice patterns (SP) for WFDC2 Gene

No ASD Table

Relevant External Links for WFDC2 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for WFDC2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for WFDC2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for WFDC2 Gene

This gene is overexpressed in Cervix - Endocervix (x11.2), Minor Salivary Gland (x10.2), and Kidney - Cortex (x10.1).

Protein differential expression in normal tissues from HIPED for WFDC2 Gene

This gene is overexpressed in Urine (35.8), Cervix (20.6), and Saliva (8.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for WFDC2 Gene

Protein tissue co-expression partners for WFDC2 Gene

NURSA nuclear receptor signaling pathways regulating expression of WFDC2 Gene:


SOURCE GeneReport for Unigene cluster for WFDC2 Gene:


mRNA Expression by UniProt/SwissProt for WFDC2 Gene:

Tissue specificity: Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.

Evidence on tissue expression from TISSUES for WFDC2 Gene

  • Intestine(4.2)
  • Saliva(4)
  • Gall bladder(2)
genes like me logo Genes that share expression patterns with WFDC2: view

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for WFDC2 Gene

Orthologs for WFDC2 Gene

This gene was present in the common ancestor of chordates.

Orthologs for WFDC2 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia WFDC2 33 34
  • 99.73 (n)
(Canis familiaris)
Mammalia WFDC2 33 34
  • 83.06 (n)
(Rattus norvegicus)
Mammalia Wfdc2 33
  • 74.32 (n)
(Mus musculus)
Mammalia Wfdc2 33 16 34
  • 74.04 (n)
(Bos Taurus)
Mammalia WFDC2 33 34
  • 73.98 (n)
(Ornithorhynchus anatinus)
Mammalia WFDC2 34
  • 60 (a)
(Monodelphis domestica)
Mammalia WFDC2 34
  • 52 (a)
(Gallus gallus)
Aves WFDC2 34
  • 6 (a)
Species where no ortholog for WFDC2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)
  • zebrafish (Danio rerio)

Evolution for WFDC2 Gene

Gene Tree for WFDC2 (if available)
Gene Tree for WFDC2 (if available)

Paralogs for WFDC2 Gene

(1) SIMAP similar genes for WFDC2 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with WFDC2: view

No data available for Paralogs for WFDC2 Gene

Variants for WFDC2 Gene

Sequence variations from dbSNP and Humsavar for WFDC2 Gene

SNP ID Clin Chr 20 pos Variation AA Info Type
rs1000129523 -- 45,468,353(+) A/C/G upstream_transcript_variant
rs1000800696 -- 45,474,918(+) T/C intron_variant
rs1000810823 -- 45,481,176(+) A/C intron_variant
rs1000837134 -- 45,481,573(+) G/A downstream_transcript_variant
rs1000905424 -- 45,478,939(+) A/G intron_variant

Structural Variations from Database of Genomic Variants (DGV) for WFDC2 Gene

Variant ID Type Subtype PubMed ID
nsv1056102 CNV loss 25217958

Variation tolerance for WFDC2 Gene

Residual Variation Intolerance Score: 46.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.08; 1.84% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for WFDC2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for WFDC2 Gene

Disorders for WFDC2 Gene

MalaCards: The human disease database

(1) MalaCards diseases for WFDC2 Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
ovarian cancer
  • ovarian cancer, somatic
- elite association - COSMIC cancer census association via MalaCards
Search WFDC2 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for WFDC2

genes like me logo Genes that share disorders with WFDC2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for WFDC2 Gene

Publications for WFDC2 Gene

  1. A major human epididymis-specific cDNA encodes a protein with sequence homology to extracellular proteinase inhibitors. (PMID: 1686187) Kirchhoff C … Krull N (Biology of reproduction 1991) 2 3 4 22 58
  2. Human epididymis protein 4 (HE4) is a secreted glycoprotein that is overexpressed by serous and endometrioid ovarian carcinomas. (PMID: 15781627) Drapkin R … Hecht JL (Cancer research 2005) 3 4 22 58
  3. The HE4 (WFDC2) protein is a biomarker for ovarian carcinoma. (PMID: 12839961) Hellström I … Hellström KE (Cancer research 2003) 3 4 22 58
  4. The putative ovarian tumour marker gene HE4 (WFDC2), is expressed in normal tissues and undergoes complex alternative splicing to yield multiple protein isoforms. (PMID: 11965550) Bingle L … Bingle CD (Oncogene 2002) 3 4 22 58
  5. Human epididymis protein-4 (HE-4): a novel cross-class protease inhibitor. (PMID: 23139753) Chhikara N … Yadav S (PloS one 2012) 3 4 58

Products for WFDC2 Gene

Sources for WFDC2 Gene

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