This gene encodes a member of the peptidase C19 family of ubiquitin-specific proteases. These deubiquitinating enzymes (DUBs) catalyze the removal of ubiquitin proteins from other proteins. The encoded protein associates with polarized mitochondria and has been shown to inhibit NF-kappa B activation and delay PARK2-mediated degradation of mitochondria. Expression of this gene i... See more...

Aliases for USP35 Gene

Aliases for USP35 Gene

  • Ubiquitin Specific Peptidase 35 2 3 5
  • Ubiquitin-Specific-Processing Protease 35 3 4
  • Ubiquitin Carboxyl-Terminal Hydrolase 35 3 4
  • Ubiquitin Specific Protease 35 2 3
  • Deubiquitinating Enzyme 35 3 4
  • Ubiquitin Thioesterase 35 3 4
  • Ubiquitin Thiolesterase 35 3
  • EC 4
  • KIAA1372 4
  • USP34 4

External Ids for USP35 Gene

Previous GeneCards Identifiers for USP35 Gene

  • GC11P078122
  • GC11P077636
  • GC11P077626
  • GC11P077577
  • GC11P077899
  • GC11P074197

Summaries for USP35 Gene

Entrez Gene Summary for USP35 Gene

  • This gene encodes a member of the peptidase C19 family of ubiquitin-specific proteases. These deubiquitinating enzymes (DUBs) catalyze the removal of ubiquitin proteins from other proteins. The encoded protein associates with polarized mitochondria and has been shown to inhibit NF-kappa B activation and delay PARK2-mediated degradation of mitochondria. Expression of this gene is upregulated by the let-7a microRNA and reduced expression has been observed in human tumor tissues. [provided by RefSeq, Jul 2017]

GeneCards Summary for USP35 Gene

USP35 (Ubiquitin Specific Peptidase 35) is a Protein Coding gene. Among its related pathways are Ubiquitin-Proteasome Dependent Proteolysis. Gene Ontology (GO) annotations related to this gene include binding and cysteine-type peptidase activity. An important paralog of this gene is USP38.

Additional gene information for USP35 Gene

No data available for CIViC Summary , UniProtKB/Swiss-Prot Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for USP35 Gene

Genomics for USP35 Gene

GeneHancer (GH) Regulatory Elements for USP35 Gene

Promoters and enhancers for USP35 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH11J078185 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 511 -0.9 -934 5.4 HNRNPK ZNF217 ZSCAN5C SIN3A TCF12 NRF1 POLR2G SP1 NCOR1 PHF8 USP35 KCTD21 KCTD21-AS1 ENSG00000241782 LINC02728 ALG8 RSF1 INTS4 piR-47352
GH11J078196 Promoter/Enhancer 0.8 EPDnew Ensembl 500.4 +8.1 8088 1.8 RBM25 KDM1A BCOR PTBP1 ZBTB33 ZMYM3 HNRNPL USP35 KCTD21 L13714-065 KCTD21-AS1
GH11J078138 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 10.9 -48.9 -48912 3 ZBTB40 ZNF217 CTCF SIN3A NRF1 TCF12 MYC POLR2G GTF2E2 PHF8 KCTD21-AS1 ALG8 lnc-NDUFC2-1 KCTD21 USP35 THRSP INTS4 piR-53144
GH11J078156 Enhancer 1.1 Ensembl ENCODE 11.6 -32.2 -32218 1.1 EP300 ZNF217 NCOR1 KDM6A TCF7 CUX1 TGIF2 ZBTB7A SKI TEAD1 KCTD21 USP35 INTS4 piR-53144 piR-57845-002 KCTD21-AS1
GH11J078256 Enhancer 1.2 Ensembl ENCODE dbSUPER 10.2 +68.5 68489 1.8 REST CTBP1 RBM25 NR2F2 NCOR1 ZNF316 ARNT CREB3L1 TRIM28 MAX ENSG00000254649 GAB2 KCTD21 USP35 NARS2 ENSG00000254459 piR-61738 piR-32325-026
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around USP35 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for USP35

Top Transcription factor binding sites by QIAGEN in the USP35 gene promoter:
  • AP-4
  • C/EBPalpha
  • Egr-4
  • NF-1
  • NF-1/L
  • Nkx2-5
  • p53
  • Pax-4a
  • SEF-1 (1)

Genomic Locations for USP35 Gene

Genomic Locations for USP35 Gene
28,833 bases
Plus strand
25,900 bases
Plus strand

Genomic View for USP35 Gene

Genes around USP35 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
USP35 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for USP35 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for USP35 Gene

Proteins for USP35 Gene

  • Protein details for USP35 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 35
    Protein Accession:

    Protein attributes for USP35 Gene

    1018 amino acids
    Molecular mass:
    113405 Da
    Quaternary structure:
    No Data Available
    • Sequence=BAA92610.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for USP35 Gene

    Alternative splice isoforms for USP35 Gene


neXtProt entry for USP35 Gene

Post-translational modifications for USP35 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for USP35 Gene

Domains & Families for USP35 Gene

Gene Families for USP35 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for USP35 Gene

  • Ubiquitin thiolesterase, family 2

Suggested Antigen Peptide Sequences for USP35 Gene

GenScript: Design optimal peptide antigens:
  • USP35 protein (A2RRA6_HUMAN)
  • Ubiquitin carboxyl-terminal hydrolase (E9PK78_HUMAN)
  • Ubiquitin-specific-processing protease 35 (UBP35_HUMAN)

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the peptidase C19 family.
  • Belongs to the peptidase C19 family.
genes like me logo Genes that share domains with USP35: view

Function for USP35 Gene

Molecular function for USP35 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).; EC=; Evidence=. ;.

Enzyme Numbers (IUBMB) for USP35 Gene

Phenotypes From GWAS Catalog for USP35 Gene

Gene Ontology (GO) - Molecular Function for USP35 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004197 cysteine-type endopeptidase activity IBA 21873635
GO:0004843 thiol-dependent ubiquitin-specific protease activity IDA 14715245
GO:0008233 peptidase activity IEA --
GO:0008234 cysteine-type peptidase activity IEA --
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with USP35: view
genes like me logo Genes that share phenotypes with USP35: view

miRNA for USP35 Gene

miRTarBase miRNAs that target USP35

Clone Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for USP35 Gene

Localization for USP35 Gene

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for USP35 gene
Compartment Confidence
nucleus 3
cytosol 2
plasma membrane 1
extracellular 1
cytoskeleton 1
mitochondrion 1
peroxisome 1
endoplasmic reticulum 1
endosome 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoli fibrillar center (2)
  • Nucleoplasm (2)
See all subcellular structures

No data available for Subcellular locations from UniProtKB/Swiss-Prot and Gene Ontology (GO) - Cellular Components for USP35 Gene

Pathways & Interactions for USP35 Gene

PathCards logo

SuperPathways for USP35 Gene

SuperPathway Contained pathways
1 Ubiquitin-Proteasome Dependent Proteolysis
genes like me logo Genes that share pathways with USP35: view

Pathways by source for USP35 Gene

1 Qiagen pathway for USP35 Gene
  • Ubiquitin-Proteasome Dependent Proteolysis

Gene Ontology (GO) - Biological Process for USP35 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis IEA --
GO:0006511 ubiquitin-dependent protein catabolic process IEA --
GO:0016579 protein deubiquitination IDA 14715245
genes like me logo Genes that share ontologies with USP35: view

No data available for SIGNOR curated interactions for USP35 Gene

Drugs & Compounds for USP35 Gene

(1) Drugs for USP35 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Water Approved Pharma 0
genes like me logo Genes that share compounds with USP35: view

Transcripts for USP35 Gene

mRNA/cDNA for USP35 Gene

10 NCBI additional mRNA sequence :
7 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for USP35 Gene

No ASD Table

Relevant External Links for USP35 Gene

GeneLoc Exon Structure for

Expression for USP35 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for USP35 Gene

Protein differential expression in normal tissues from HIPED for USP35 Gene

This gene is overexpressed in Platelet (36.3) and Liver, secretome (25.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for USP35 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for USP35

SOURCE GeneReport for Unigene cluster for USP35 Gene:


mRNA Expression by UniProt/SwissProt for USP35 Gene:

Tissue specificity: Expressed in testis, pancreas and skeletal muscle.

Evidence on tissue expression from TISSUES for USP35 Gene

  • Nervous system(4.2)
genes like me logo Genes that share expression patterns with USP35: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for USP35 Gene

Orthologs for USP35 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for USP35 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia USP35 30
  • 99.09 (n)
(Canis familiaris)
Mammalia USP35 31 30
  • 88.35 (n)
(Bos Taurus)
Mammalia USP35 31
  • 88 (a)
(Mus musculus)
Mammalia Usp35 17 31 30
  • 85.93 (n)
(Rattus norvegicus)
Mammalia Usp35 30
  • 85.73 (n)
(Monodelphis domestica)
Mammalia USP35 31
  • 75 (a)
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 34 (a)
(Gallus gallus)
Aves USP35 30
  • 67.41 (n)
-- 31
  • 64 (a)
-- 31
  • 54 (a)
(Anolis carolinensis)
Reptilia USP35 31
  • 62 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia usp35 30
  • 63.47 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP010080 30
  • 48.87 (n)
fruit fly
(Drosophila melanogaster)
Insecta CG8830 31 30
  • 48.52 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes UBP13 31
  • 17 (a)
UBP9 31
  • 17 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 35 (a)
Species where no ortholog for USP35 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)
  • zebrafish (Danio rerio)

Evolution for USP35 Gene

Gene Tree for USP35 (if available)
Gene Tree for USP35 (if available)
Evolutionary constrained regions (ECRs) for USP35: view image

Paralogs for USP35 Gene

(1) SIMAP similar genes for USP35 Gene using alignment to 6 proteins:

  • E9PK78_HUMAN
genes like me logo Genes that share paralogs with USP35: view

Variants for USP35 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for USP35 Gene

SNP ID Clinical significance and condition Chr 11 pos Variation AA Info Type
708109 Benign: not provided 78,210,201(+) C/T SYNONYMOUS_VARIANT
713425 Benign: not provided 78,219,281(+) G/A SYNONYMOUS_VARIANT
722439 Benign: not provided 78,222,097(+) G/A INTRON_VARIANT
787094 Benign: not provided 78,219,356(+) C/T SYNONYMOUS_VARIANT
rs2510044 - p.Val236Met

Additional dbSNP identifiers (rs#s) for USP35 Gene

Structural Variations from Database of Genomic Variants (DGV) for USP35 Gene

Variant ID Type Subtype PubMed ID
esv33345 CNV gain 17666407
nsv1036793 CNV gain 25217958
nsv1127212 CNV deletion 24896259
nsv1132346 CNV duplication 24896259
nsv516503 CNV loss 19592680
nsv520740 CNV gain 19592680
nsv527066 CNV gain 19592680
nsv826000 CNV loss 20364138

Variation tolerance for USP35 Gene

Residual Variation Intolerance Score: 89.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 11.60; 93.08% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for USP35 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for USP35 Gene

Disorders for USP35 Gene

Additional Disease Information for USP35

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for USP35 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for USP35 Gene

Publications for USP35 Gene

  1. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PMID: 20628086) Bailey SD … DREAM investigators (Diabetes care 2010) 3 41 54
  2. Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases. (PMID: 14715245) Quesada V … López-Otín C (Biochemical and biophysical research communications 2004) 3 4 54
  3. Human and mouse proteases: a comparative genomic approach. (PMID: 12838346) Puente XS … López-Otín C (Nature reviews. Genetics 2003) 2 3 54
  4. Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. (PMID: 10718198) Nagase T … Ohara O (DNA research : an international journal for rapid publication of reports on genes and genomes 2000) 3 4 54
  5. Discovery and Characterization of ZUFSP/ZUP1, a Distinct Deubiquitinase Class Important for Genome Stability. (PMID: 29576527) Kwasna D … Kulathu Y (Molecular cell 2018) 3 54

Products for USP35 Gene

Sources for USP35 Gene