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Aliases for USP25 Gene

Aliases for USP25 Gene

  • Ubiquitin Specific Peptidase 25 2 3 5
  • Deubiquitinating Enzyme 25 3 4
  • Ubiquitin Thioesterase 25 3 4
  • USP On Chromosome 21 3 4
  • USP21 3 4
  • Ubiquitin-Specific Processing Protease 25 3
  • Ubiquitin-Specific-Processing Protease 25 4
  • Ubiquitin Carboxyl-Terminal Hydrolase 25 3
  • Ubiquitin Specific Protease 25 2
  • Ubiquitin Thiolesterase 25 3
  • EC 4
  • EC 56

External Ids for USP25 Gene

Previous GeneCards Identifiers for USP25 Gene

  • GC21P013766
  • GC21P016024
  • GC21P017102
  • GC21P002481

Summaries for USP25 Gene

Entrez Gene Summary for USP25 Gene

  • Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]

GeneCards Summary for USP25 Gene

USP25 (Ubiquitin Specific Peptidase 25) is a Protein Coding gene. Among its related pathways are Metabolism of proteins and Ubiquitin-Proteasome Dependent Proteolysis. Gene Ontology (GO) annotations related to this gene include peptidase activity and thiol-dependent ubiquitin-specific protease activity. An important paralog of this gene is USP28.

UniProtKB/Swiss-Prot for USP25 Gene

  • Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both Lys-48- and Lys-63-linked tetraubiquitin chains.

  • The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.

Additional gene information for USP25 Gene

No data available for CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for USP25 Gene

Genomics for USP25 Gene

GeneHancer (GH) Regulatory Elements for USP25 Gene

Promoters and enhancers for USP25 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH21I015729 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE 563.7 +2.9 2866 7.2 HDGF PKNOX1 CLOCK SMAD1 ARNT ARID4B SIN3A FEZF1 YY1 ZNF213 USP25 RBPMSLP
GH21I015753 Enhancer 1.2 FANTOM5 Ensembl ENCODE 20.2 +23.9 23854 2.8 USF1 FOXA2 SAP130 MAX SIN3A ZNF766 ZNF316 POLR2A CBFA2T2 NFE2 USP25 RBPMSLP GC21P015884
GH21I015706 Enhancer 0.9 Ensembl ENCODE 20.1 -23.1 -23079 1.2 MEIS2 JUN NEUROD1 FOSL1 CTBP1 GATA3 RCOR1 FOS FOSL2 SMARCE1 USP25 RAD23BP3
GH21I015788 Enhancer 0.7 Ensembl ENCODE 24.1 +59.2 59247 1.4 STAT1 PKNOX1 GATA3 JUN CEBPB FOXA1 USP25 RBPMSLP GC21P015884
GH21I015826 Enhancer 0.8 ENCODE 19.3 +96.6 96598 0.7 FOXA2 ARNT RAD21 RFX5 ARID2 SCRT2 ZNF143 BCLAF1 CREM ZNF654 USP25 GC21P015884 RBPMSLP
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around USP25 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the USP25 gene promoter:

Genomic Locations for USP25 Gene

Genomic Locations for USP25 Gene
150,126 bases
Plus strand

Genomic View for USP25 Gene

Genes around USP25 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
USP25 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for USP25 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for USP25 Gene

Proteins for USP25 Gene

  • Protein details for USP25 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 25
    Protein Accession:
    Secondary Accessions:
    • C0LSZ0
    • Q6DHZ9
    • Q9H9W1

    Protein attributes for USP25 Gene

    1055 amino acids
    Molecular mass:
    122218 Da
    Quaternary structure:
    • Homodimer or oligomer. Interacts with ACTA1 (via its C-terminus); the interaction occurs for all isoforms but is strongest for isoform USP25m in muscle differentiating cells. Interacts (isoform USP25m only) with MYBPC1; the interaction prevents proteasomal degradation of MYBPC1. Interacts (isoform USP25m only) with FLNC (via filament repeats 17-18, 20-21 and 24). Interacts with GAPDH. Interacts with SUMO3; the interaction sumoylates efficiently USP25. Interacts with SUMO2; the interaction sumoylates efficiently USP25. Interacts with SUMO1; the interaction only weakly sumoylates USP25. Interacts with SYK; phosphorylates USP25 and regulates USP25 intracellular levels.

    Three dimensional structures from OCA and Proteopedia for USP25 Gene

    Alternative splice isoforms for USP25 Gene


neXtProt entry for USP25 Gene

Selected DME Specific Peptides for USP25 Gene


Post-translational modifications for USP25 Gene

  • Acetylated.
  • Phosphorylation in the C-terminal by SYK regulates USP25 cellular levels.
  • Preferentially monoubiquitinated but can also be polyubiquitinated. Autodeubiquitinated. Ubiquitination activates the enzymatic activity either by preventing sumoylation or by allowing novel interactions.
  • Sumoylation impairs binding to and hydrolysis of ubiquitin chains. Sumoylated preferentially with SUMO2 or SUMO3. Desumoylated by SENP1. Regulated by ubiquitination on the same residue.
  • Ubiquitination at posLast=1515, posLast=201201, isoforms=2, 3250, isoforms=2, 3254, posLast=312312, isoforms=2, 3313, isoforms=2, 3392, posLast=444444, isoforms=2, 3520, posLast=584584, isoforms=2, 3630, isoforms=2, 3698, posLast=715715, and isoforms=2, 3721

Antibody Products

  • Abcam antibodies for USP25
  • Santa Cruz Biotechnology (SCBT) Antibodies for USP25

Domains & Families for USP25 Gene

Gene Families for USP25 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for USP25 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the peptidase C19 family.
  • Belongs to the peptidase C19 family.
genes like me logo Genes that share domains with USP25: view

Function for USP25 Gene

Molecular function for USP25 Gene

GENATLAS Biochemistry:
ubiquitin specific protease 25,expressed in testis,pancreas,kidney,liver,lung,brain,heart,skeletal muscle,encoding two alternatively spliced isoforms,deubiquitin enzyme family
UniProtKB/Swiss-Prot CatalyticActivity:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
UniProtKB/Swiss-Prot Function:
Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both Lys-48- and Lys-63-linked tetraubiquitin chains.
UniProtKB/Swiss-Prot Function:
The muscle-specific isoform (USP25m) may have a role in the regulation of muscular differentiation and function.
UniProtKB/Swiss-Prot Induction:
The muscle-specific isoform (USP25m) is up-regulated during myocyte differentiation. Levels increase up to 100-fold towards completion of differentiation.

Enzyme Numbers (IUBMB) for USP25 Gene

Phenotypes From GWAS Catalog for USP25 Gene

Gene Ontology (GO) - Molecular Function for USP25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004843 thiol-dependent ubiquitin-specific protease activity IMP 18538659
GO:0005515 protein binding IPI 18538659
GO:0008233 peptidase activity TAS 10644437
GO:0008234 cysteine-type peptidase activity IEA --
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with USP25: view
genes like me logo Genes that share phenotypes with USP25: view

Clone Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for USP25 Gene

Localization for USP25 Gene

Subcellular locations from UniProtKB/Swiss-Prot for USP25 Gene

Isoform USP25m: Cytoplasm. Nucleus. Note=Some transient punctuate nuclear location in myotubes during myocyte development. {ECO:0000250}.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for USP25 gene
Compartment Confidence
endoplasmic reticulum 5
cytosol 5
nucleus 4
mitochondrion 2
peroxisome 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for USP25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005737 cytoplasm IEA --
GO:0005783 endoplasmic reticulum IDA 22590560
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with USP25: view

Pathways & Interactions for USP25 Gene

genes like me logo Genes that share pathways with USP25: view

Pathways by source for USP25 Gene

Gene Ontology (GO) - Biological Process for USP25 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006464 cellular protein modification process TAS 10644437
GO:0006508 proteolysis TAS 10644437
GO:0006511 ubiquitin-dependent protein catabolic process IEA --
GO:0016579 protein deubiquitination IDA,TAS --
GO:0070536 protein K63-linked deubiquitination IMP 18538659
genes like me logo Genes that share ontologies with USP25: view

No data available for SIGNOR curated interactions for USP25 Gene

Drugs & Compounds for USP25 Gene

(1) Drugs for USP25 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Water Approved Pharma 0
genes like me logo Genes that share compounds with USP25: view

Transcripts for USP25 Gene

Unigene Clusters for USP25 Gene

Ubiquitin specific peptidase 25:
Representative Sequences:

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for USP25 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10a · 10b · 10c ^ 11 ^ 12a · 12b ^ 13 ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20 ^
SP1: - -
SP2: - - -
SP3: - - - - - - - - - - - - - - - - - -
SP5: -
SP6: -
SP7: -

ExUns: 21 ^ 22 ^ 23 ^ 24 ^ 25a · 25b ^ 26a · 26b
SP1: -
SP2: -
SP3: - - -
SP4: -

Relevant External Links for USP25 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for USP25 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for USP25 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for USP25 Gene

This gene is overexpressed in Muscle - Skeletal (x4.7).

Protein differential expression in normal tissues from HIPED for USP25 Gene

This gene is overexpressed in Lymph node (13.9), Monocytes (8.8), Plasma (7.6), and CD8 Tcells (6.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for USP25 Gene

NURSA nuclear receptor signaling pathways regulating expression of USP25 Gene:


SOURCE GeneReport for Unigene cluster for USP25 Gene:


mRNA Expression by UniProt/SwissProt for USP25 Gene:

Tissue specificity: Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.

Evidence on tissue expression from TISSUES for USP25 Gene

  • Nervous system(4.3)
  • Muscle(2)
genes like me logo Genes that share expression patterns with USP25: view

No data available for Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for USP25 Gene

Orthologs for USP25 Gene

This gene was present in the common ancestor of animals.

Orthologs for USP25 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia USP25 33 34
  • 99.69 (n)
(Canis familiaris)
Mammalia USP25 33 34
  • 94.6 (n)
(Bos Taurus)
Mammalia USP25 33 34
  • 93.71 (n)
(Rattus norvegicus)
Mammalia Usp25 33
  • 87.79 (n)
(Mus musculus)
Mammalia Usp25 33 16 34
  • 86.9 (n)
(Ornithorhynchus anatinus)
Mammalia USP25 34
  • 81 (a)
(Monodelphis domestica)
Mammalia USP25 34
  • 80 (a)
(Gallus gallus)
Aves USP25 33 34
  • 81.97 (n)
(Anolis carolinensis)
Reptilia USP25 34
  • 65 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia usp25 33
  • 74.99 (n)
Str.4178 33
African clawed frog
(Xenopus laevis)
Amphibia Xl.18074 33
(Danio rerio)
Actinopterygii usp25 33 34
  • 68.32 (n)
Dr.12045 33
(Caenorhabditis elegans)
Secernentea K02C4.3 34
  • 17 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 35 (a)
Species where no ortholog for USP25 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for USP25 Gene

Gene Tree for USP25 (if available)
Gene Tree for USP25 (if available)

Paralogs for USP25 Gene

Paralogs for USP25 Gene

(1) SIMAP similar genes for USP25 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with USP25: view

Variants for USP25 Gene

Sequence variations from dbSNP and Humsavar for USP25 Gene

SNP ID Clin Chr 21 pos Variation AA Info Type
rs1000019297 -- 15,739,723(+) C/A genic_upstream_transcript_variant, intron_variant
rs1000032276 -- 15,867,192(+) T/G genic_downstream_transcript_variant, intron_variant
rs1000042006 -- 15,825,718(+) T/C intron_variant
rs1000046758 -- 15,784,496(+) C/T genic_upstream_transcript_variant, intron_variant
rs1000057539 -- 15,866,945(+) T/C genic_downstream_transcript_variant, intron_variant

Structural Variations from Database of Genomic Variants (DGV) for USP25 Gene

Variant ID Type Subtype PubMed ID
nsv834050 CNV gain 17160897
nsv587076 CNV gain 21841781
nsv459099 CNV gain 19166990
nsv3462 CNV insertion 18451855
nsv3461 CNV insertion 18451855
nsv1133523 CNV deletion 24896259
nsv1132560 CNV duplication 24896259
esv3568111 CNV loss 25503493
esv2762093 CNV gain 21179565
esv275197 CNV gain+loss 21479260
dgv4390n100 CNV gain 25217958

Variation tolerance for USP25 Gene

Residual Variation Intolerance Score: 6.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.46; 54.96% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for USP25 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for USP25 Gene

Disorders for USP25 Gene

Additional Disease Information for USP25

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for USP25 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for USP25 Gene

Publications for USP25 Gene

  1. Functional interaction between the ubiquitin-specific protease 25 and the SYK tyrosine kinase. (PMID: 19909739) Cholay M … Daviet L (Experimental cell research 2010) 3 4 22 58
  2. Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25. (PMID: 18538659) Meulmeester E … Melchior F (Molecular cell 2008) 3 4 22 58
  3. The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins. (PMID: 16501887) Bosch-Comas A … Marfany G (Cellular and molecular life sciences : CMLS 2006) 3 4 22 58
  4. Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25. (PMID: 11597335) Valero R … Marfany G (Genome biology 2001) 3 4 22 58
  5. Narrowing of the region of allelic loss in 21q11-21 in squamous non-small cell lung carcinoma and cloning of a novel ubiquitin-specific protease gene from the deleted segment. (PMID: 10612803) Groet J … Nizetic D (Genes, chromosomes & cancer 2000) 2 3 4 58

Products for USP25 Gene

Sources for USP25 Gene

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