The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enz... See more...

Aliases for UBE2M Gene

Aliases for UBE2M Gene

  • Ubiquitin Conjugating Enzyme E2 M 2 3 5
  • Ubiquitin-Conjugating Enzyme E2M (UBC12 Homolog, Yeast) 2 3
  • NEDD8-Conjugating Enzyme Ubc12 3 4
  • NEDD8 Carrier Protein 3 4
  • UBC12 3 4
  • Ubiquitin-Conjugating Enzyme E2M (Homologous To Yeast UBC12) 2
  • Epididymis Secretory Sperm Binding Protein 3
  • Ubiquitin-Conjugating Enzyme E2 M 4
  • Ubiquitin Conjugating Enzyme E2M 3
  • Ubiquitin-Conjugating Enzyme E2M 2
  • Ubiquitin Carrier Protein M 3
  • Ubiquitin-Protein Ligase M 3
  • NEDD8 Protein Ligase 3
  • Yeast UBC12 Homolog 3
  • EC 2.3.2.- 4
  • EC 6.3.2 52
  • UBC-RS2 3
  • HUbc12 3

External Ids for UBE2M Gene

Previous GeneCards Identifiers for UBE2M Gene

  • GC19M059965
  • GC19M059521
  • GC19M063742
  • GC19M063758
  • GC19M059067

Summaries for UBE2M Gene

Entrez Gene Summary for UBE2M Gene

  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]

GeneCards Summary for UBE2M Gene

UBE2M (Ubiquitin Conjugating Enzyme E2 M) is a Protein Coding gene. Diseases associated with UBE2M include Familial Hypertension. Among its related pathways are Class I MHC mediated antigen processing and presentation and Ubiquitin-Proteasome Dependent Proteolysis. Gene Ontology (GO) annotations related to this gene include ubiquitin-protein transferase activity and ubiquitin protein ligase activity.

UniProtKB/Swiss-Prot Summary for UBE2M Gene

  • Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

Gene Wiki entry for UBE2M Gene

Additional gene information for UBE2M Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for UBE2M Gene

Genomics for UBE2M Gene

GeneHancer (GH) Regulatory Elements for UBE2M Gene

Promoters and enhancers for UBE2M Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around UBE2M on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for UBE2M

Top Transcription factor binding sites by QIAGEN in the UBE2M gene promoter:
  • AML1a

Genomic Locations for UBE2M Gene

Genomic Locations for UBE2M Gene
chr19:58,555,712-58,558,976
(GRCh38/hg38)
Size:
3,265 bases
Orientation:
Minus strand
chr19:59,067,079-59,070,343
(GRCh37/hg19)
Size:
3,265 bases
Orientation:
Minus strand

Genomic View for UBE2M Gene

Genes around UBE2M on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
UBE2M Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for UBE2M Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for UBE2M Gene

Proteins for UBE2M Gene

  • Protein details for UBE2M Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P61081-UBC12_HUMAN
    Recommended name:
    NEDD8-conjugating enzyme Ubc12
    Protein Accession:
    P61081
    Secondary Accessions:
    • O76069
    • Q8VC50

    Protein attributes for UBE2M Gene

    Size:
    183 amino acids
    Molecular mass:
    20900 Da
    Quaternary structure:
    • Interacts with UBA3 and RBX1. Interacts (acetylated at N-terminal methionine) with DCUN1D1 (via DCUN1 domain) (PubMed:28581483).

    Three dimensional structures from OCA and Proteopedia for UBE2M Gene

neXtProt entry for UBE2M Gene

Selected DME Specific Peptides for UBE2M Gene

P61081:
  • ELNLPKTC
  • DDLLNFKLVICPDEGFYK
  • KKASAAQLRIQKDINELNLP
  • CLNILREDW

Post-translational modifications for UBE2M Gene

  • The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
  • Ubiquitination at Lys3, Lys8, Lys36, Lys75, and Lys81
  • Modification sites at PhosphoSitePlus

Other Protein References for UBE2M Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for UBE2M Gene

Gene Families for UBE2M Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for UBE2M Gene

Blocks:
  • Ubiquitin-conjugating enzymes
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for UBE2M Gene

GenScript: Design optimal peptide antigens:
  • Ubiquitin-conjugating enzyme E2 M (UBC12_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P61081

UniProtKB/Swiss-Prot:

UBC12_HUMAN :
  • Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
Domain:
  • Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
Family:
  • Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
genes like me logo Genes that share domains with UBE2M: view

Function for UBE2M Gene

Molecular function for UBE2M Gene

UniProtKB/Swiss-Prot Function:
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

Enzyme Numbers (IUBMB) for UBE2M Gene

Phenotypes From GWAS Catalog for UBE2M Gene

Gene Ontology (GO) - Molecular Function for UBE2M Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity TAS 9694792
GO:0005515 protein binding IPI 15361859
GO:0005524 ATP binding IEA --
GO:0016740 transferase activity IEA --
GO:0019788 NEDD8 transferase activity TAS --
genes like me logo Genes that share ontologies with UBE2M: view
genes like me logo Genes that share phenotypes with UBE2M: view

Animal Models for UBE2M Gene

MGI Knock Outs for UBE2M:
  • Ube2m Ube2m<tm1.1(KOMP)Wtsi>

Animal Model Products

CRISPR Products

miRNA for UBE2M Gene

miRTarBase miRNAs that target UBE2M

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for UBE2M

Clone Products

  • Addgene plasmids for UBE2M

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for UBE2M Gene

Localization for UBE2M Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for UBE2M gene
Compartment Confidence
nucleus 5
cytosol 5
plasma membrane 1
extracellular 1
cytoskeleton 1
mitochondrion 1
endoplasmic reticulum 1
endosome 1
lysosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (2)
  • Nuclear bodies (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for UBE2M Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm TAS --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with UBE2M: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for UBE2M Gene

Pathways & Interactions for UBE2M Gene

genes like me logo Genes that share pathways with UBE2M: view

Pathways by source for UBE2M Gene

1 R&D Systems pathway for UBE2M Gene
2 Qiagen pathways for UBE2M Gene
  • Remodeling of Adherens Junctions
  • Ubiquitin-Proteasome Dependent Proteolysis

UniProtKB/Swiss-Prot P61081-UBC12_HUMAN

  • Pathway: Protein modification; protein neddylation.

Gene Ontology (GO) - Biological Process for UBE2M Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006464 cellular protein modification process TAS 9694792
GO:0016567 protein ubiquitination IEA --
GO:0043525 positive regulation of neuron apoptotic process IEA --
GO:0043687 post-translational protein modification TAS --
GO:0045116 protein neddylation IBA,IEA --
genes like me logo Genes that share ontologies with UBE2M: view

No data available for SIGNOR curated interactions for UBE2M Gene

Drugs & Compounds for UBE2M Gene

(3) Drugs for UBE2M Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Adenosine monophosphate Approved, Investigational Nutra 0
ATP Investigational Nutra Agonist, Activator, Full agonist, Antagonist, Pore Blocker, Potentiation 0

(1) Additional Compounds for UBE2M Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • [(ho)2P(O)OP(O)(OH)2]
  • Acide diphosphorique
  • Diphosphorsaeure
  • H4P2O7
  • PYROphosphATE
14000-31-8
genes like me logo Genes that share compounds with UBE2M: view

Transcripts for UBE2M Gene

mRNA/cDNA for UBE2M Gene

1 REFSEQ mRNAs :
10 NCBI additional mRNA sequence :
5 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for UBE2M

Clone Products

  • Addgene plasmids for UBE2M

Alternative Splicing Database (ASD) splice patterns (SP) for UBE2M Gene

ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b
SP1: -
SP2: -
SP3:
SP4:

Relevant External Links for UBE2M Gene

GeneLoc Exon Structure for
UBE2M

Expression for UBE2M Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for UBE2M Gene

Protein differential expression in normal tissues from HIPED for UBE2M Gene

This gene is overexpressed in Peripheral blood mononuclear cells (7.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for UBE2M Gene



Protein tissue co-expression partners for UBE2M Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for UBE2M

SOURCE GeneReport for Unigene cluster for UBE2M Gene:

Hs.406068

Evidence on tissue expression from TISSUES for UBE2M Gene

  • Skin(4.3)
  • Nervous system(2.8)
  • Muscle(2.3)
  • Intestine(2.1)
  • Heart(2)
genes like me logo Genes that share expression patterns with UBE2M: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for UBE2M Gene

Orthologs for UBE2M Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for UBE2M Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia UBE2M 31 30
  • 99.77 (n)
OneToOne
dog
(Canis familiaris)
Mammalia UBE2M 31 30
  • 97.27 (n)
OneToOne
cow
(Bos Taurus)
Mammalia UBE2M 31 30
  • 96.54 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ube2m 30
  • 94.9 (n)
mouse
(Mus musculus)
Mammalia Ube2m 17 31 30
  • 94.35 (n)
chicken
(Gallus gallus)
Aves UBE2M 31
  • 47 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia UBE2M 31
  • 98 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia ube2m 30
  • 81.42 (n)
MGC75750 30
zebrafish
(Danio rerio)
Actinopterygii wu:fb58e05 30
  • 81.24 (n)
wufb58e05 30
fruit fly
(Drosophila melanogaster)
Insecta CG7375 32
  • 75 (a)
Ubc12 31 30
  • 73.56 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005916 30
  • 70.13 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ADL035C 30
  • 55.49 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes UBC12 33 31 30
  • 50.93 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0C17248g 30
  • 47.25 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT2G18600 30
  • 59.89 (n)
soybean
(Glycine max)
eudicotyledons Gma.17973 30
rice
(Oryza sativa)
Liliopsida Os08g0374100 30
  • 59.52 (n)
Os.14656 30
wheat
(Triticum aestivum)
Liliopsida Ta.3716 30
corn
(Zea mays)
Liliopsida Zm.4335 30
barley
(Hordeum vulgare)
Liliopsida Hv.3803 30
bread mold
(Neurospora crassa)
Ascomycetes NCU03610 30
  • 62.3 (n)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.4912 31
  • 62 (a)
OneToOne
Cin.1924 30
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes ubc12 30
  • 54.17 (n)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.1924 30
Species where no ortholog for UBE2M was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • worm (Caenorhabditis elegans)

Evolution for UBE2M Gene

ENSEMBL:
Gene Tree for UBE2M (if available)
TreeFam:
Gene Tree for UBE2M (if available)
Aminode:
Evolutionary constrained regions (ECRs) for UBE2M: view image

Paralogs for UBE2M Gene

(20) SIMAP similar genes for UBE2M Gene using alignment to 3 proteins:

  • UBC12_HUMAN
  • M0QX69_HUMAN
  • M0QYI6_HUMAN

Pseudogenes.org Pseudogenes for UBE2M Gene

genes like me logo Genes that share paralogs with UBE2M: view

No data available for Paralogs for UBE2M Gene

Variants for UBE2M Gene

Additional dbSNP identifiers (rs#s) for UBE2M Gene

Structural Variations from Database of Genomic Variants (DGV) for UBE2M Gene

Variant ID Type Subtype PubMed ID
dgv3687n100 CNV gain 25217958
dgv3688n100 CNV gain 25217958
nsv1057077 CNV gain 25217958
nsv1057206 CNV gain 25217958
nsv1160670 CNV deletion 26073780
nsv515731 CNV gain+loss 19592680
nsv828657 CNV gain 20364138
nsv952156 CNV deletion 24416366

Variation tolerance for UBE2M Gene

Residual Variation Intolerance Score: 49.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.22; 5.00% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for UBE2M Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
UBE2M

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for UBE2M Gene

Disorders for UBE2M Gene

MalaCards: The human disease database

(1) MalaCards diseases for UBE2M Gene - From: GeneCards

Disorder Aliases PubMed IDs
familial hypertension
  • genetic hypertension
- elite association - COSMIC cancer census association via MalaCards
Search UBE2M in MalaCards View complete list of genes associated with diseases

Additional Disease Information for UBE2M

genes like me logo Genes that share disorders with UBE2M: view

No data available for UniProtKB/Swiss-Prot and Genatlas for UBE2M Gene

Publications for UBE2M Gene

  1. A dominant-negative UBC12 mutant sequesters NEDD8 and inhibits NEDD8 conjugation in vivo. (PMID: 10828074) Wada H … Kamitani T (The Journal of biological chemistry 2000) 3 4 23 54
  2. A new NEDD8-ligating system for cullin-4A. (PMID: 9694792) Osaka F … Kato S (Genes & development 1998) 2 3 4 54
  3. Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase. (PMID: 28581483) Scott DC … Guy RK (Nature chemical biology 2017) 3 4 54
  4. Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes. (PMID: 23201271) Monda JK … Schulman BA (Structure (London, England : 1993) 2013) 3 4 54
  5. N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex. (PMID: 21940857) Scott DC … Schulman BA (Science (New York, N.Y.) 2011) 3 4 54

Products for UBE2M Gene

Sources for UBE2M Gene