The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enz... See more...

Aliases for UBE2I Gene

Aliases for UBE2I Gene

  • Ubiquitin Conjugating Enzyme E2 I 2 3 5
  • Ubiquitin-Conjugating Enzyme E2I (Homologous To Yeast UBC9) 2 3
  • Ubiquitin-Conjugating Enzyme E2I (UBC9 Homolog, Yeast) 2 3
  • RING-Type E3 SUMO Transferase UBC9 3 4
  • SUMO-Conjugating Enzyme UBC9 3 4
  • Ubiquitin Carrier Protein 9 3 4
  • Ubiquitin Carrier Protein I 3 4
  • Ubiquitin-Protein Ligase I 3 4
  • SUMO-Protein Ligase 3 4
  • UBC9 3 4
  • P18 3 4
  • Ubiquitin-Like Protein SUMO-1 Conjugating Enzyme 3
  • Ubiquitin-Conjugating Enzyme UbcE2A 3
  • Ubiquitin-Conjugating Enzyme E2 I 4
  • Ubiquitin Conjugating Enzyme E2I 3
  • Ubiquitin-Conjugating Enzyme E2I 2
  • Ubiquitin Conjugating Enzyme 9 3
  • Ubiquitin-Protein Ligase E2I 3
  • SUMO-1-Protein Ligase 3
  • EC 2.3.2.- 4
  • C358B7.1 3
  • EC 6.3.2 52
  • UBCE9 4

External Ids for UBE2I Gene

Previous GeneCards Identifiers for UBE2I Gene

  • GC16P001375
  • GC16P001299
  • GC16P001287
  • GC16P001359
  • GC16P001310

Summaries for UBE2I Gene

Entrez Gene Summary for UBE2I Gene

  • The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

GeneCards Summary for UBE2I Gene

UBE2I (Ubiquitin Conjugating Enzyme E2 I) is a Protein Coding gene. Diseases associated with UBE2I include Microphthalmia and Lung Cancer Susceptibility 3. Among its related pathways are Metabolism of proteins and Proteolysis Putative SUMO-1 pathway. Gene Ontology (GO) annotations related to this gene include ligase activity. An important paralog of this gene is CDC34.

UniProtKB/Swiss-Prot Summary for UBE2I Gene

  • Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity (PubMed:26620705).

Gene Wiki entry for UBE2I Gene

Additional gene information for UBE2I Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for UBE2I Gene

Genomics for UBE2I Gene

GeneHancer (GH) Regulatory Elements for UBE2I Gene

Promoters and enhancers for UBE2I Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around UBE2I on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for UBE2I

Top Transcription factor binding sites by QIAGEN in the UBE2I gene promoter:
  • AP-1

Genomic Locations for UBE2I Gene

Genomic Locations for UBE2I Gene
chr16:1,308,880-1,327,018
(GRCh38/hg38)
Size:
18,139 bases
Orientation:
Plus strand
chr16:1,355,548-1,377,019
(GRCh37/hg19)
Size:
21,472 bases
Orientation:
Plus strand

Genomic View for UBE2I Gene

Genes around UBE2I on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
UBE2I Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for UBE2I Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for UBE2I Gene

Proteins for UBE2I Gene

  • Protein details for UBE2I Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P63279-UBC9_HUMAN
    Recommended name:
    SUMO-conjugating enzyme UBC9
    Protein Accession:
    P63279
    Secondary Accessions:
    • D3DU69
    • P50550
    • Q15698
    • Q59GX1
    • Q86VB3

    Protein attributes for UBE2I Gene

    Size:
    158 amino acids
    Molecular mass:
    18007 Da
    Quaternary structure:
    • Interacts with SETX (PubMed:24105744). Interacts with HIPK1, HIPK2, PPM1J, RASD2 and TCF3. Interacts with NR2C1; the interaction promotes its sumoylation (By similarity). Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B. Interacts with DNMT1. Interacts with FOXL2. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain. Interacts with PML-RARA oncoprotein (via the coiled-colied domain); the interaction is required for sumoylation of the PML-RARA oncoprotein. Interacts with IPO13. Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains. Interacts with FHIT. Interacts with PRKRA and p53/TP53 (By similarity). Interacts with UHRF2. Interacts with NR3C1 and this interaction is enhanced in the presence of RWDD3. Interacts with MTA1. Interacts with ZNF451. Identified in a complex with SUMO2 and UBE2I, where one ZNF451 interacts with one UBE2I and two SUMO2 chains, one bound to the UBE2I active site and the other to another region of the same UBE2I molecule. Interacts with CPEB3 (By similarity). Interacts with SUMO1P1/SUMO5 (PubMed:27211601).
    • (Microbial infection) Interacts with human herpesvirus 6 IE2.
    • (Microbial infection) Interacts with human adenovirus early E1A protein; this interaction interferes with polysumoylation.
    • (Microbial infection) Interacts with Epstein-barr virus protein LMP1.
    SequenceCaution:
    • Sequence=AAH51289.3; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=BAD92225.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for UBE2I Gene

neXtProt entry for UBE2I Gene

Selected DME Specific Peptides for UBE2I Gene

P63279:
  • HPNVYPSGT
  • VAVPTKNPDGTMNLMN
  • PAITIKQILLGIQ
  • PAQAEAYTIYCQNRVEYEKR
  • GIQELLNEPNIQ
  • DWRPAIT

Post-translational modifications for UBE2I Gene

  • Phosphorylation at Ser-71 significantly enhances SUMOylation activity.
  • Ubiquitination at Lys59 and Lys65
  • Modification sites at PhosphoSitePlus

Domains & Families for UBE2I Gene

Gene Families for UBE2I Gene

HGNC:
Human Protein Atlas (HPA):
  • Cancer-related genes
  • Plasma proteins
  • Predicted intracellular proteins
  • Transporters

Protein Domains for UBE2I Gene

Blocks:
  • Ubiquitin-conjugating enzymes
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for UBE2I Gene

GenScript: Design optimal peptide antigens:
  • Ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast), isoform CRA_b (Q7KZS0_HUMAN)
  • p18 (UBC9_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P63279

UniProtKB/Swiss-Prot:

UBC9_HUMAN :
  • Belongs to the ubiquitin-conjugating enzyme family.
Family:
  • Belongs to the ubiquitin-conjugating enzyme family.
genes like me logo Genes that share domains with UBE2I: view

Function for UBE2I Gene

Molecular function for UBE2I Gene

UniProtKB/Swiss-Prot Function:
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity (PubMed:26620705).
GENATLAS Biochemistry:
ubiquitin conjugating enzyme E2I,homolog to yeast UBC9,associating with RAD51,RAD52 proteins in the double strand RAD51/RAD52 repair pathway,also associating with UBL1 and PT53,involved in DNA recombination and essential for cell-cycle progression

Enzyme Numbers (IUBMB) for UBE2I Gene

Phenotypes From GWAS Catalog for UBE2I Gene

Gene Ontology (GO) - Molecular Function for UBE2I Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0003723 RNA binding HDA 22658674
GO:0004842 ubiquitin-protein transferase activity IEA --
GO:0005515 protein binding IPI 9885291
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with UBE2I: view
genes like me logo Genes that share phenotypes with UBE2I: view

Animal Models for UBE2I Gene

MGI Knock Outs for UBE2I:

Animal Model Products

  • Taconic Biosciences Mouse Models for UBE2I

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for UBE2I

Clone Products

  • Addgene plasmids for UBE2I

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for UBE2I Gene

Localization for UBE2I Gene

Subcellular locations from UniProtKB/Swiss-Prot for UBE2I Gene

Nucleus. Cytoplasm. Note=Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity). {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for UBE2I gene
Compartment Confidence
nucleus 5
cytosol 5
plasma membrane 2
extracellular 2
cytoskeleton 2
mitochondrion 2
endoplasmic reticulum 2
peroxisome 1
endosome 1
lysosome 1
golgi apparatus 1

Gene Ontology (GO) - Cellular Components for UBE2I Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000795 synaptonemal complex TAS 8610150
GO:0001650 fibrillar center IEA --
GO:0005634 nucleus IDA,IBA 9223278
GO:0005635 nuclear envelope TAS --
GO:0005654 nucleoplasm TAS --
genes like me logo Genes that share ontologies with UBE2I: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for UBE2I Gene

Pathways & Interactions for UBE2I Gene

genes like me logo Genes that share pathways with UBE2I: view

Pathways by source for UBE2I Gene

3 GeneGo (Thomson Reuters) pathways for UBE2I Gene
  • Development_Glucocorticoid receptor signaling
  • DNA damage Role of SUMO in p53 regulation
  • Proteolysis Putative SUMO-1 pathway
6 Qiagen pathways for UBE2I Gene
  • Fas Signaling
  • Glucocorticoid Receptor Signaling
  • JAK-STAT Pathway
  • Remodeling of Adherens Junctions
  • SUMO Pathway
1 GeneTex pathway for UBE2I Gene

UniProtKB/Swiss-Prot P63279-UBC9_HUMAN

  • Pathway: Protein modification; protein sumoylation.

SIGNOR curated interactions for UBE2I Gene

Activates:
Inactivates:
Is inactivated by:

Gene Ontology (GO) - Biological Process for UBE2I Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000122 negative regulation of transcription by RNA polymerase II TAS --
GO:0006464 cellular protein modification process TAS 8798754
GO:0006511 ubiquitin-dependent protein catabolic process TAS 8565643
GO:0007049 cell cycle IEA --
GO:0007059 chromosome segregation IEA --
genes like me logo Genes that share ontologies with UBE2I: view

Drugs & Compounds for UBE2I Gene

(9) Drugs for UBE2I Gene - From: PharmGKB, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Cisplatin Approved Pharma Inhibits DNA synthesis,chemotherapy drug, Potent pro-apoptotic anticancer agent; activates caspase-3, Platinum 3311
Irinotecan Approved, Investigational Pharma Topoisomerase I inhibitor, TOPO I inhibitor, Topoisomerase 1 Inhibitors 1353
Phosphoric acid Approved Pharma 0
Adenosine monophosphate Approved, Investigational Nutra 0
ATP Investigational Nutra Agonist, Activator, Full agonist, Antagonist, Pore Blocker, Potentiation 0

(3) Additional Compounds for UBE2I Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • [(ho)2P(O)OP(O)(OH)2]
  • Acide diphosphorique
  • Diphosphorsaeure
  • H4P2O7
  • PYROphosphATE
14000-31-8
genes like me logo Genes that share compounds with UBE2I: view

Transcripts for UBE2I Gene

mRNA/cDNA for UBE2I Gene

4 REFSEQ mRNAs :
36 NCBI additional mRNA sequence :
19 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for UBE2I

Clone Products

  • Addgene plasmids for UBE2I

Alternative Splicing Database (ASD) splice patterns (SP) for UBE2I Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4a · 4b · 4c · 4d ^ 5a · 5b ^ 6a · 6b ^ 7a · 7b · 7c ^ 8 ^ 9a · 9b ^ 10 ^ 11a · 11b · 11c · 11d · 11e
SP1: - - - - - - - - - -
SP2: - - - - - - - - - - -
SP3: - - - - - - -
SP4: - - - - - - - - -
SP5: - - - - - - - - -
SP6: - - - -
SP7: - - - - -
SP8: - - - - - - - -
SP9: - - - - - -
SP10: - - - - - -
SP11: - -
SP12: - -
SP13:
SP14:

Relevant External Links for UBE2I Gene

GeneLoc Exon Structure for
UBE2I

Expression for UBE2I Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for UBE2I Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for UBE2I Gene



Protein tissue co-expression partners for UBE2I Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for UBE2I

SOURCE GeneReport for Unigene cluster for UBE2I Gene:

Hs.302903

mRNA Expression by UniProt/SwissProt for UBE2I Gene:

P63279-UBC9_HUMAN
Tissue specificity: Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Evidence on tissue expression from TISSUES for UBE2I Gene

  • Nervous system(4.8)
  • Lung(4.6)
  • Liver(4.4)
  • Stomach(3.3)
  • Intestine(3.1)
  • Pancreas(2.7)
  • Kidney(2.4)
  • Muscle(2.3)
  • Adrenal gland(2.2)
  • Heart(2)
genes like me logo Genes that share expression patterns with UBE2I: view

No data available for mRNA differential expression in normal tissues , Protein differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for UBE2I Gene

Orthologs for UBE2I Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for UBE2I Gene

Organism Taxonomy Gene Similarity Type Details
platypus
(Ornithorhynchus anatinus)
Mammalia UBE2I 31
  • 100 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia UBE2I 31
  • 100 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia UBE2I 31 30
  • 98.95 (n)
OneToOne
dog
(Canis familiaris)
Mammalia UBE2I 31 30
  • 91.98 (n)
OneToOne
cow
(Bos Taurus)
Mammalia UBE2I 31 30
  • 90.72 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Ube2i 17 31 30
  • 89.24 (n)
rat
(Rattus norvegicus)
Mammalia Ube2i 30
  • 89.24 (n)
chicken
(Gallus gallus)
Aves UBE2I 31 30
  • 87.13 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia UBE2I 31
  • 100 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia ube2i 30
  • 85.65 (n)
Str.8764 30
African clawed frog
(Xenopus laevis)
Amphibia ube2i-prov 30
zebrafish
(Danio rerio)
Actinopterygii ube2i2 31 31 30
  • 81.32 (n)
OneToMany
-- 30
fruit fly
(Drosophila melanogaster)
Insecta lwr 31 32 30
  • 70.89 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP011076 30
  • 68.99 (n)
worm
(Caenorhabditis elegans)
Secernentea ubc-9 31 32
  • 72 (a)
OneToOne
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0E05039g 30
  • 57.91 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AER056C 30
  • 56.62 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes UBC9 33 31 30
  • 54.91 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons SCE1 30
  • 62.77 (n)