This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromoso... See more...

Aliases for TTK Gene

Aliases for TTK Gene

  • TTK Protein Kinase 2 3 5
  • Phosphotyrosine Picked Threonine-Protein Kinase 3 4
  • Dual Specificity Protein Kinase TTK 3 4
  • Monopolar Spindle 1 Kinase 2 3
  • Cancer/Testis Antigen 96 2 3
  • MPS1L1 3 4
  • MPS1 3 4
  • PYT 3 4
  • Phosphotyrosine Picked Threonine Kinase 3
  • Monopolar Spindle 1-Like 1 3
  • EC 2.7.12.1 4
  • CT96 3
  • MPH1 3
  • ESK 3

External Ids for TTK Gene

Previous GeneCards Identifiers for TTK Gene

  • GC06P080657
  • GC06P080709
  • GC06P080771
  • GC06P077937
  • GC06P080713

Summaries for TTK Gene

Entrez Gene Summary for TTK Gene

  • This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]

GeneCards Summary for TTK Gene

TTK (TTK Protein Kinase) is a Protein Coding gene. Diseases associated with TTK include Monieziasis. Among its related pathways are Cell cycle and DNA Damage. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is TLK2.

UniProtKB/Swiss-Prot Summary for TTK Gene

  • Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.

Tocris Summary for TTK Gene

  • Monopolar spindle 1 (Mps1) kinase, also known as TTK protein kinase, is a mitotic kinase that is critical in the regulation of cell division, as it is involved in controlling the progression of cells through mitosis. It is widely found, but is not ubiquitous, in eukaryotes.

Gene Wiki entry for TTK Gene

Additional gene information for TTK Gene

No data available for CIViC Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for TTK Gene

Genomics for TTK Gene

GeneHancer (GH) Regulatory Elements for TTK Gene

Promoters and enhancers for TTK Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH06J080002 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 500.7 +0.7 693 3.6 ZBTB40 ZNF217 EP300 SIN3A NRF1 POLR2G SP1 PHF8 JUND TEAD4 lnc-ELOVL4-4 TTK ELOVL4 ENSG00000272129 BCKDHB ENSG00000233967 piR-33605-397
GH06J079964 Enhancer 0.7 Ensembl ENCODE dbSUPER 10.8 -38.7 -38739 2.1 ATF2 ATF7 RPL35AP18 lnc-TTK-2 TTK BCKDHB ELOVL4 ENSG00000287816
GH06J080029 Enhancer 0.5 Ensembl 11 +25.9 25914 0.4 BATF IKZF1 NR2F1 SPI1 NFIC BCLAF1 RUNX3 TTK piR-33605-397 lnc-ELOVL4-4
GH06J079941 Enhancer 0.5 Ensembl 10.1 -61.3 -61286 2.8 USF1 FOS ZBTB48 JUN ZNF577 PATZ1 JUND PRDM1 BCKDHB TTK ELOVL4 RF01045-131
GH06J080143 Enhancer 0.8 Ensembl ENCODE 6.6 +140.4 140414 3.8 CTCF FOS CEBPB GTF2F1 STAT3 JUND EP300 RAD21 SMC3 NFIB BCKDHB ENSG00000272129 TTK piR-46847-225 RF00017-5637
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around TTK on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for TTK

Top Transcription factor binding sites by QIAGEN in the TTK gene promoter:
  • CBF(2)
  • CBF-A
  • CBF-B
  • CBF-C
  • CP1A
  • CP1C
  • FOXL1
  • NF-Y
  • NF-YA
  • Nkx2-5

Genomic Locations for TTK Gene

Genomic Locations for TTK Gene
chr6:80,003,887-80,042,527
(GRCh38/hg38)
Size:
38,641 bases
Orientation:
Plus strand
chr6:80,713,604-80,752,244
(GRCh37/hg19)
Size:
38,641 bases
Orientation:
Plus strand

Genomic View for TTK Gene

Genes around TTK on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
TTK Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for TTK Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for TTK Gene

Proteins for TTK Gene

  • Protein details for TTK Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P33981-TTK_HUMAN
    Recommended name:
    Dual specificity protein kinase TTK
    Protein Accession:
    P33981
    Secondary Accessions:
    • A8K8U5
    • B2RDW2
    • E1P543
    • Q15272
    • Q5TCS0
    • Q9BW51
    • Q9NTM0

    Protein attributes for TTK Gene

    Size:
    857 amino acids
    Molecular mass:
    97072 Da
    Quaternary structure:
    • Interacts with TPR; the interactions occurs in a microtubule-independent manner.
    SequenceCaution:
    • Sequence=AAA61239.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for TTK Gene

    Alternative splice isoforms for TTK Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for TTK Gene

Post-translational modifications for TTK Gene

  • Ubiquitination at Lys19, Lys86, Lys434, Lys452, Lys538, Lys810, and Lys830
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for TTK Gene

Domains & Families for TTK Gene

Gene Families for TTK Gene

IUPHAR :
Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for TTK Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ77795, highly similar to Homo sapiens TTK protein kinase, mRNA (A8K8U5_HUMAN)
  • TTK protein kinase (Q5TCS1_HUMAN)
  • Phosphotyrosine picked threonine-protein kinase (TTK_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P33981

UniProtKB/Swiss-Prot:

TTK_HUMAN :
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Family:
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
genes like me logo Genes that share domains with TTK: view

Function for TTK Gene

Molecular function for TTK Gene

UniProtKB/Swiss-Prot Function:
Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.12.1;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.12.1;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858, ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.12.1;.
UniProtKB/Swiss-Prot EnzymeRegulation:
Inhibited by the ATP-competitive kinase inhibitor, SP600125.
GENATLAS Biochemistry:
TTK serine/threonine kinase,expressed in testis and thymus,yeast Mps1and CDC20/Slp1 homolog,functioning at the mitotic spindle checkpoint

Enzyme Numbers (IUBMB) for TTK Gene

Phenotypes From GWAS Catalog for TTK Gene

Gene Ontology (GO) - Molecular Function for TTK Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IEA --
GO:0004674 protein serine/threonine kinase activity TAS,IBA 21873635
GO:0004712 protein serine/threonine/tyrosine kinase activity IEA,IBA 21873635
GO:0004713 protein tyrosine kinase activity IEA,TAS --
GO:0005515 protein binding IPI,IEA 19273613
genes like me logo Genes that share ontologies with TTK: view
genes like me logo Genes that share phenotypes with TTK: view

Animal Model Products

  • Taconic Biosciences Mouse Models for TTK

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for TTK

Clone Products

  • Addgene plasmids for TTK

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for TTK Gene

Localization for TTK Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for TTK gene
Compartment Confidence
nucleus 5
cytoskeleton 4
cytosol 3
plasma membrane 2
extracellular 2
mitochondrion 2
peroxisome 1
endoplasmic reticulum 1
endosome 1
lysosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (2)
  • Nucleoli (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for TTK Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000776 kinetochore IEA,IBA 21873635
GO:0005634 nucleus IBA 21873635
GO:0005737 cytoplasm IDA 25468996
GO:0005819 spindle IBA,TAS 21873635
GO:0016020 membrane HDA 19946888
genes like me logo Genes that share ontologies with TTK: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for TTK Gene

Pathways & Interactions for TTK Gene

PathCards logo

SuperPathways for TTK Gene

genes like me logo Genes that share pathways with TTK: view

Pathways by source for TTK Gene

1 BioSystems pathway for TTK Gene
1 KEGG pathway for TTK Gene
1 Cell Signaling Technology pathway for TTK Gene

SIGNOR curated interactions for TTK Gene

Activates:
Inactivates:
Is activated by:
Is inactivated by:
Other effect:

Gene Ontology (GO) - Biological Process for TTK Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IEA --
GO:0007051 spindle organization TAS 10366450
GO:0007052 mitotic spindle organization TAS 7737118
GO:0007059 chromosome segregation IBA 21873635
GO:0007093 mitotic cell cycle checkpoint IEA --
genes like me logo Genes that share ontologies with TTK: view

Drugs & Compounds for TTK Gene

(14) Drugs for TTK Gene - From: DrugBank, ClinicalTrials, ApexBio, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
fostamatinib Approved, Investigational Pharma Target, inhibitor Kinase Inhibitors 0
2,6-Dihydroanthra/1,9-Cd/Pyrazol-6-One Experimental Pharma Target 0
BOS172722 Experimental Pharma Target, inhibitor 0
Immunologic Factors Pharma 30591
Vaccines Pharma 7866

(6) ApexBio Compounds for TTK Gene

Compound Action Cas Number
AZ3146 Mps1 inhibitor,potent and selective 1124329-14-1
MPI-0479605 Mps1 inhibitor,selective and ATP competitive 1246529-32-7
Mps1-IN-1 1125593-20-5
Mps1-IN-2 Mps1 kinase inhibitor 1228817-38-6
Mps1-IN-3 1609584-72-6
NMS-P715 MPS1 kinase inhibitor 1202055-34-2
genes like me logo Genes that share compounds with TTK: view

Drug Products

Transcripts for TTK Gene

mRNA/cDNA for TTK Gene

2 REFSEQ mRNAs :
9 NCBI additional mRNA sequence :
11 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for TTK

Clone Products

  • Addgene plasmids for TTK

Alternative Splicing Database (ASD) splice patterns (SP) for TTK Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c · 6d ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15a · 15b ^ 16 ^ 17 ^ 18a · 18b ^
SP1: - - -
SP2: -
SP3: -
SP4: - -
SP5: - -
SP6: -
SP7:

ExUns: 19a · 19b ^ 20a · 20b ^ 21a · 21b ^ 22a · 22b ^ 23 ^ 24
SP1: -
SP2:
SP3: - -
SP4:
SP5:
SP6:
SP7:

Relevant External Links for TTK Gene

GeneLoc Exon Structure for
TTK

Expression for TTK Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for TTK Gene

mRNA differential expression in normal tissues according to GTEx for TTK Gene

This gene is overexpressed in Testis (x13.1).

Protein differential expression in normal tissues from HIPED for TTK Gene

This gene is overexpressed in Cervix (22.7), Bone (15.5), Heart (10.2), Testis (9.9), and Placenta (7.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for TTK Gene



Protein tissue co-expression partners for TTK Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for TTK

SOURCE GeneReport for Unigene cluster for TTK Gene:

Hs.169840

mRNA Expression by UniProt/SwissProt for TTK Gene:

P33981-TTK_HUMAN
Tissue specificity: Present in rapidly proliferating cell lines.

Evidence on tissue expression from TISSUES for TTK Gene

  • Eye(4)
  • Nervous system(2)
genes like me logo Genes that share expression patterns with TTK: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and Phenotype-based relationships between genes and organs from Gene ORGANizer for TTK Gene

Orthologs for TTK Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for TTK Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia TTK 31 30
  • 99.38 (n)
OneToOne
dog
(Canis familiaris)
Mammalia TTK 31 30
  • 90.11 (n)
OneToOne
cow
(Bos Taurus)
Mammalia TTK 31 30
  • 89.21 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ttk 30
  • 82.55 (n)
mouse
(Mus musculus)
Mammalia Ttk 17 31 30
  • 81.2 (n)
oppossum
(Monodelphis domestica)
Mammalia TTK 31
  • 74 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia TTK 31
  • 66 (a)
OneToOne
chicken
(Gallus gallus)
Aves TTK 31 30
  • 70.02 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia TTK 31
  • 58 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia ttk 30
  • 63.16 (n)
African clawed frog
(Xenopus laevis)
Amphibia LOC398219 30
zebrafish
(Danio rerio)
Actinopterygii ttk 31 30
  • 54.23 (n)
OneToOne
mps1 30
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.2362 30
fruit fly
(Drosophila melanogaster)
Insecta CG7643 32
  • 44 (a)
ald 31
  • 29 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes MPS1 33 31
  • 25 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 49 (a)
OneToOne
Species where no ortholog for TTK was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for TTK Gene

ENSEMBL:
Gene Tree for TTK (if available)
TreeFam:
Gene Tree for TTK (if available)
Aminode:
Evolutionary constrained regions (ECRs) for TTK: view image

Paralogs for TTK Gene

Paralogs for TTK Gene

Pseudogenes.org Pseudogenes for TTK Gene

genes like me logo Genes that share paralogs with TTK: view

Variants for TTK Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for TTK Gene

SNP ID Clinical significance and condition Chr 06 pos Variation AA Info Type
716028 Benign: not provided 80,040,680(+) A/C MISSENSE_VARIANT
748445 Likely Benign: not provided 80,011,435(+) A/G SYNONYMOUS_VARIANT
775239 Likely Benign: not provided 80,026,389(+) C/G SYNONYMOUS_VARIANT
787538 Benign: not provided 80,040,289(+) T/A INTRON_VARIANT
788996 Benign: not provided 80,022,445(+) G/A SYNONYMOUS_VARIANT

Additional dbSNP identifiers (rs#s) for TTK Gene

Structural Variations from Database of Genomic Variants (DGV) for TTK Gene

Variant ID Type Subtype PubMed ID
nsv5370 CNV deletion 18451855

Variation tolerance for TTK Gene

Residual Variation Intolerance Score: 56.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.97; 49.48% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for TTK Gene

Human Gene Mutation Database (HGMD)
TTK
SNPedia medical, phenotypic, and genealogical associations of SNPs for
TTK

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for TTK Gene

Disorders for TTK Gene

MalaCards: The human disease database

(1) MalaCards diseases for TTK Gene - From: DISEASES

Disorder Aliases PubMed IDs
monieziasis
- elite association - COSMIC cancer census association via MalaCards
Search TTK in MalaCards View complete list of genes associated with diseases

Additional Disease Information for TTK

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with TTK: view

No data available for UniProtKB/Swiss-Prot and Genatlas for TTK Gene

Publications for TTK Gene

  1. Expression of TTK, a novel human protein kinase, is associated with cell proliferation. (PMID: 1639825) Mills GB … Hogg D (The Journal of biological chemistry 1992) 2 3 4 23 54
  2. Mutational analysis of TTK gene in gastric and colorectal cancers with microsatellite instability. (PMID: 20057968) Ahn CH … Lee SH (Cancer research and treatment : official journal of Korean Cancer Association 2009) 3 23 41 54
  3. Breast cancer risk associated with genotypic polymorphism of the mitotic checkpoint genes: a multigenic study on cancer susceptibility. (PMID: 17210994) Lo YL … Shen CY (Carcinogenesis 2007) 3 23 41 54
  4. Genetic variation in the major mitotic checkpoint genes does not affect familial breast cancer risk. (PMID: 17268814) Vaclavicek A … Försti A (Breast cancer research and treatment 2007) 3 23 41 54
  5. Centrosome-related genes, genetic variation, and risk of breast cancer. (PMID: 20508983) Olson JE … Couch FJ (Breast cancer research and treatment 2011) 3 41 54

Products for TTK Gene

Sources for TTK Gene