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Aliases for TSSK4 Gene

Aliases for TSSK4 Gene

  • Testis Specific Serine Kinase 4 2 3 5
  • Serine/Threonine-Protein Kinase 22E 3 4
  • Serine/Threonine Kinase 22E 2 3
  • C14orf20 3 4
  • STK22E 3 4
  • TSK-4 3 4
  • TSSK5 3 4
  • Testis-Specific Serine/Threonine-Protein Kinase 4 3
  • Chromosome 14 Open Reading Frame 20 2
  • Testis-Specific Kinase 4 4
  • EC 4
  • EC 2.7.11 56
  • TSSK-4 4
  • TSK4 3

External Ids for TSSK4 Gene

Previous HGNC Symbols for TSSK4 Gene

  • C14orf20
  • STK22E

Previous GeneCards Identifiers for TSSK4 Gene

  • GC14P023745
  • GC14P024674
  • GC14P004789

Summaries for TSSK4 Gene

Entrez Gene Summary for TSSK4 Gene

  • This gene encodes a member of the testis-specific serine/threonine kinase family. The encoded protein is thought to be involved in spermatogenesis via stimulation of the CREB/CRE responsive pathway through phosphorylation of the cAMP responsive element binding protein transcription factor. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

GeneCards Summary for TSSK4 Gene

TSSK4 (Testis Specific Serine Kinase 4) is a Protein Coding gene. Among its related pathways are Sweet Taste Signaling. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is TSSK1B.

UniProtKB/Swiss-Prot for TSSK4 Gene

  • May be involved in a signaling pathway during male germ cell development or mature sperm function (By similarity). Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.

Additional gene information for TSSK4 Gene

No data available for CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for TSSK4 Gene

Genomics for TSSK4 Gene

GeneHancer (GH) Regulatory Elements for TSSK4 Gene

Promoters and enhancers for TSSK4 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH14I024205 Promoter 0.5 EPDnew 550.8 +0.1 147 0.1 TSSK4 PIR41663 ENSG00000254692
GH14I024206 Enhancer 0.5 ENCODE 550.8 +2.0 1976 1.4 SCRT1 ZNF282 SSRP1 POLR2A IKZF1 SCRT2 PIR41663 TSSK4 ENSG00000254692
GH14I024270 Promoter/Enhancer 2.4 EPDnew FANTOM5 Ensembl ENCODE 35.4 +67.3 67296 5.4 HDGF PKNOX1 FOXA2 SMAD1 MLX ARNT ZFP64 ARID4B SIN3A DMAP1 LOC102725044 RABGGTA TSSK4 IPO4 RNF31 HOMEZ KHNYN NOP9 NGDN DHRS1
GH14I023953 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 36.1 -251.4 -251426 2 HDGF SMAD1 MLX ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 SLC30A9 DHRS4 ACIN1 SDR39U1 NGDN RNA5SP383 RNF31 KHNYN TSSK4 DHRS4-AS1 PABPN1
GH14I024113 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 24.6 -90.7 -90690 3.1 CLOCK MLX ZFP64 FEZF1 DMAP1 YY1 SLC30A9 ZNF213 ZNF143 SP3 DCAF11 NRL RNF31 NFATC4 IPO4 HOMEZ RNA5SP383 TSSK4 TM9SF1 KHNYN
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around TSSK4 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the TSSK4 gene promoter:

Genomic Locations for TSSK4 Gene

Genomic Locations for TSSK4 Gene
2,833 bases
Plus strand

Genomic View for TSSK4 Gene

Genes around TSSK4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
TSSK4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for TSSK4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for TSSK4 Gene

Proteins for TSSK4 Gene

  • Protein details for TSSK4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Testis-specific serine/threonine-protein kinase 4
    Protein Accession:
    Secondary Accessions:
    • Q2TA60
    • Q6ZNM2

    Protein attributes for TSSK4 Gene

    328 amino acids
    Molecular mass:
    37454 Da
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for TSSK4 Gene


neXtProt entry for TSSK4 Gene

Selected DME Specific Peptides for TSSK4 Gene


Post-translational modifications for TSSK4 Gene

Domains & Families for TSSK4 Gene

Gene Families for TSSK4 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins
  • Predicted membrane proteins

Suggested Antigen Peptide Sequences for TSSK4 Gene

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
  • Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
genes like me logo Genes that share domains with TSSK4: view

Function for TSSK4 Gene

Molecular function for TSSK4 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Activated by phosphorylation on Thr-197, potentially by autophosphorylation.
UniProtKB/Swiss-Prot Function:
May be involved in a signaling pathway during male germ cell development or mature sperm function (By similarity). Phosphorylates CREB1 on Ser-133 and stimulates downstream signaling.

Enzyme Numbers (IUBMB) for TSSK4 Gene

Phenotypes From GWAS Catalog for TSSK4 Gene

Gene Ontology (GO) - Molecular Function for TSSK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding ISS --
GO:0004672 protein kinase activity IEA --
GO:0004674 protein serine/threonine kinase activity ISS --
GO:0005515 protein binding IPI 15964553
GO:0005524 ATP binding ISS,IEA --
genes like me logo Genes that share ontologies with TSSK4: view
genes like me logo Genes that share phenotypes with TSSK4: view

Animal Model Products

  • Taconic Biosciences Mouse Models for TSSK4

Clone Products

No data available for Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for TSSK4 Gene

Localization for TSSK4 Gene

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for TSSK4 gene
Compartment Confidence
nucleus 4
cytosol 3
peroxisome 2
extracellular 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cell Junctions (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for TSSK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IBA --
GO:0005737 cytoplasm IBA --
genes like me logo Genes that share ontologies with TSSK4: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for TSSK4 Gene

Pathways & Interactions for TSSK4 Gene

SuperPathways for TSSK4 Gene

SuperPathway Contained pathways
1 Sweet Taste Signaling
genes like me logo Genes that share pathways with TSSK4: view

Pathways by source for TSSK4 Gene

1 Qiagen pathway for TSSK4 Gene

SIGNOR curated interactions for TSSK4 Gene


Gene Ontology (GO) - Biological Process for TSSK4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IEA,ISS --
GO:0007275 multicellular organism development IEA --
GO:0007283 spermatogenesis IEA --
GO:0016310 phosphorylation IEA --
GO:0030154 cell differentiation IEA --
genes like me logo Genes that share ontologies with TSSK4: view

Drugs & Compounds for TSSK4 Gene

(2) Drugs for TSSK4 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Magnesium Approved Nutra 0
ATP Investigational Nutra Agonist 0

(1) Additional Compounds for TSSK4 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
genes like me logo Genes that share compounds with TSSK4: view

Transcripts for TSSK4 Gene

Unigene Clusters for TSSK4 Gene

Testis-specific serine kinase 4:
Representative Sequences:

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for TSSK4 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3a · 3b · 3c · 3d ^ 4a · 4b · 4c
SP1: -
SP2: - -
SP3: -
SP4: -

Relevant External Links for TSSK4 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for TSSK4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for TSSK4 Gene

mRNA differential expression in normal tissues according to GTEx for TSSK4 Gene

This gene is overexpressed in Testis (x12.4).

Protein differential expression in normal tissues from HIPED for TSSK4 Gene

This gene is overexpressed in CD8 Tcells (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for TSSK4 Gene

Protein tissue co-expression partners for TSSK4 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of TSSK4 Gene:


SOURCE GeneReport for Unigene cluster for TSSK4 Gene:


mRNA Expression by UniProt/SwissProt for TSSK4 Gene:

Tissue specificity: Expressed only in the testis.
genes like me logo Genes that share expression patterns with TSSK4: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , Evidence on tissue expression from TISSUES and Phenotype-based relationships between genes and organs from Gene ORGANizer for TSSK4 Gene

Orthologs for TSSK4 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for TSSK4 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia TSSK4 33 34
  • 99.31 (n)
(Canis familiaris)
Mammalia TSSK4 33 34
  • 90.04 (n)
(Bos Taurus)
Mammalia TSSK4 33 34
  • 89.88 (n)
(Rattus norvegicus)
Mammalia Tssk4 33
  • 89.84 (n)
(Mus musculus)
Mammalia Tssk4 33 16 34
  • 88.46 (n)
(Monodelphis domestica)
Mammalia TSSK4 34
  • 79 (a)
(Anolis carolinensis)
Reptilia TSSK4 34
  • 74 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG9222 33 34
  • 54.06 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008735 33
  • 50.52 (n)
(Caenorhabditis elegans)
Secernentea W02B12.12 33
  • 45.07 (n)
F23C8.8 34
  • 27 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes FRK1 34
  • 11 (a)
KIN4 34
  • 11 (a)
-- 34
  • 10 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 35 (a)
Species where no ortholog for TSSK4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • zebrafish (Danio rerio)

Evolution for TSSK4 Gene

Gene Tree for TSSK4 (if available)
Gene Tree for TSSK4 (if available)

Paralogs for TSSK4 Gene

Paralogs for TSSK4 Gene

genes like me logo Genes that share paralogs with TSSK4: view

Variants for TSSK4 Gene

Sequence variations from dbSNP and Humsavar for TSSK4 Gene

SNP ID Clin Chr 14 pos Variation AA Info Type
rs1000661628 -- 24,205,644(+) T/C 5_prime_UTR_variant, genic_upstream_transcript_variant, upstream_transcript_variant
rs1000730648 -- 24,207,257(+) T/C coding_sequence_variant, synonymous_variant
rs1000813099 -- 24,205,547(+) C/G 5_prime_UTR_variant, genic_upstream_transcript_variant, upstream_transcript_variant
rs1001339053 -- 24,208,738(+) A/C/G downstream_transcript_variant
rs1001487624 -- 24,204,831(+) G/A upstream_transcript_variant

Variation tolerance for TSSK4 Gene

Residual Variation Intolerance Score: 62.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 5.22; 69.94% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for TSSK4 Gene

Human Gene Mutation Database (HGMD)
SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for TSSK4 Gene

Disorders for TSSK4 Gene

Additional Disease Information for TSSK4

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for TSSK4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for TSSK4 Gene

Publications for TSSK4 Gene

  1. Evaluation of 172 candidate polymorphisms for association with oligozoospermia or azoospermia in a large cohort of men of European descent. (PMID: 20378615) Aston KI … Carrell DT (Human reproduction (Oxford, England) 2010) 3 44 58
  2. Mutation screening and association study of the TSSK4 Gene in Chinese infertile men with impaired spermatogenesis. (PMID: 18390560) Su D … Zhang S (Journal of andrology 2008) 3 44 58
  3. TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway. (PMID: 15964553) Chen X … Yu L (Biochemical and biophysical research communications 2005) 3 4 58
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4 58
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 58

Products for TSSK4 Gene

Sources for TSSK4 Gene

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