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The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains,... See more...
Aliases for TRIM28 Gene
Aliases for TRIM28 Gene
- Tripartite Motif Containing 28 2 3 5
- RNF96 2 3 4
- TIF1B 2 3 4
- KAP1 2 3 4
- Protein Phosphatase 1, Regulatory Subunit 157 2 3
- RING-Type E3 Ubiquitin Transferase TIF1-Beta 3 4
- Transcription Intermediary Factor 1-Beta 3 4
- E3 SUMO-Protein Ligase TRIM28 3 4
- KRAB-Interacting Protein 1 3 4
- Nuclear Corepressor KAP-1 3 4
- RING Finger Protein 96 3 4
External Ids for TRIM28 Gene
- HGNC: 16384
- NCBI Entrez Gene: 10155
- Ensembl: ENSG00000130726
- OMIM®: 601742
- UniProtKB/Swiss-Prot: Q13263
Previous GeneCards Identifiers for TRIM28 Gene
- GC19P059954
- GC19P059509
- GC19P063731
- GC19P063747
- GC19P063553
- GC19P059055
- GC19P055366
Summaries for TRIM28 Gene
-
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
GeneCards Summary for TRIM28 Gene
TRIM28 (Tripartite Motif Containing 28) is a Protein Coding gene. Diseases associated with TRIM28 include Wilms Tumor 1 and Wilms Tumor 5. Among its related pathways are Corticotropin-releasing hormone signaling pathway and p53 pathway. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding. An important paralog of this gene is TRIM33.
UniProtKB/Swiss-Prot Summary for TRIM28 Gene
-
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610). Acts as a corepressor for ZFP568 (By similarity).
-
(Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.
Additional gene information for TRIM28 Gene
- HGNC (16384)
- NCBI Entrez Gene (10155)
- Ensembl (ENSG00000130726)
- OMIM® (601742)
- UniProtKB/Swiss-Prot (Q13263)
- Open Targets Platform(ENSG00000130726)
- Alliance of Genome Resources
- Search for TRIM28 at DataMed
- Search for TRIM28 at HumanCyc
No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for TRIM28 Gene
Genomics for TRIM28 Gene
GeneHancer (GH) Regulatory Elements Pubs
Promoters and enhancers for TRIM28 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication |
Request up-to-date GeneHancer data (full dataset)
GeneHancers around TRIM28 on the GeneHancer Hub at the UCSC Golden Path
Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for TRIM28
- Top Transcription factor binding sites by QIAGEN in the TRIM28 gene promoter:
-
- AP-4
- CUTL1
- HNF-4alpha1
- MRF-2
- MZF-1
- Pax-3
- PPAR-gamma1
- PPAR-gamma2
- SRF
- SRF (504 AA)
Genomic Locations for TRIM28 Gene
Latest Assembly
- chr19:58,544,064-58,550,722
- (GRCh38/hg38)
- Size:
- 6,659 bases
- Orientation:
- Plus strand
Previous Assembly
- chr19:59,055,431-59,062,082
- (GRCh37/hg19 by Entrez Gene)
- Size:
- 6,652 bases
- Orientation:
- Plus strand
Genomic View for TRIM28 Gene
Genes around TRIM28 on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 19q13.43 by HGNC
- 19q13.43 by Entrez Gene
- 19q13.43 by Ensembl
TRIM28 Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for TRIM28 Gene
Proteins for TRIM28 Gene
-
Protein details for TRIM28 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- Q13263-TIF1B_HUMAN
- Recommended name:
- Transcription intermediary factor 1-beta
- Protein Accession:
- Q13263
- O00677
- Q7Z632
- Q93040
- Q96IM1
Protein attributes for TRIM28 Gene
- Size:
- 835 amino acids
- Molecular mass:
- 88550 Da
- Quaternary structure:
-
- Interacts with SETX (PubMed:23149945). Oligomer; the RBCC domain homotrimerizes and interacts with one molecule of KRAB to form the KRAB-KAP1 corepressor complex. Binding to a KRAB domain is an absolute requirement for silencing gene expression. Interacts with CEBPB and NR3C1. Interacts with a number of KRAB-ZFP proteins including ZNF10, ZFP53, ZFP68, ZNF382 and ZNF256. Interacts with NCOR1, NR3C1 and CHD3. Interacts with CEBPB (via the RING-type and PHD-type zinc fingers). Component of a ternary complex that includes TRIM28, a HP1 protein (CBX1, CBX3 OR CBX5), a KRAB domain-containing protein, and DNA. Interacts with CBX5 (via the PxVxL motif); the interaction occurs in interphase nuclei and competes for binding POGZ. Interacts with POGZ; the interaction competes for interaction with CBX5. Interacts with SETDB1; the interaction is enhanced by KAP1 sumoylation, stimulates SETDB1 histone methyltransferase activity and gene silencing. Interacts (via the PHD-type zinc finger) with UBE2I; the interaction is required for sumoylation and repressor activity. Component of the TRIM28/KAP1-ERBB4-MDM2 complex involved in connecting growth factor and DNA damage responses. Interacts directly with ERBB4; the interaction represses ERBB4-mediated transcription activity. Interacts with MDM2; the interaction contributes to p53/TP53 inactivation. Component of the TRIM28/KAP1-MDM2-p53/TP53; involved in regulating p53/TP53 stabilization and activity. Interacts (via the leucine zipper alpha helical coiled-coil) with E2F1 (central region); the interaction inhibits E2F1 acetylation and transcriptional activity. Interacts with PPP1CA; the interaction dephosphorylates TRIM28 at Ser-824 and forms a complex at the p21 promoter site. Interacts with PPP1CB; the interaction is weak but is increased on dephosphorylation at Ser-824. Interacts with FES/FPS. Interacts with SMARCAD1. Interacts with, and sumoylates IRF7. Interacts with MAGEC2. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with AICDA (By similarity). Interacts (via the RBCC domain) with KOX1 (via the KRAB domain), ZNF268 (via the KRAB domain) and ZNF300 (via the KRAB domain); the interactions increase KOX1, ZNF268 and ZNF300 nuclear localization activities. The large PER complex involved in the histone methylation is composed of at least PER2, CBX3, TRIM28, SUV39H1 and/or SUV39H2; CBX3 mediates the formation of the complex. Interacts with isoform 2 of ZFP90. Forms a complex with FOXP3 in the presence of isoform 2 of ZFP90. Interacts with NR4A3; the interactions potentiates NR4A3 activity on NurRE promoter (By similarity). Interacts (unphosphorylated or phosphorylated form) with ZBTB1 (via BTB domain) (PubMed:24657165). Probably part of a corepressor complex containing ZNF304, TRIM28, SETDB1 and DNMT1 (PubMed:24623306). Interacts with ATRX. Forms a complex with ATRX, SETDB1 and ZNF274 (PubMed:27029610). Interacts with ZFP568; the interaction mediates ZFP568 transcriptional repression activity (By similarity). Interacts with RRP1B (PubMed:19710015). Interacts with CRY1 (By similarity).
- (Microbial infection) Interacts with herpes virus 8 protein LANA1; this interaction facilitates establishment of viral latency.
Three dimensional structures from OCA and Proteopedia for TRIM28 Gene
Protein Expression for TRIM28 Gene
Post-translational modifications for TRIM28 Gene
- ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes.
- Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21.
- Auto-ubiquitinated; enhanced by MAGEA2 and MAGEC2.
- Citrullinated by PADI4.
-
Ubiquitination at Lys31, Lys127, Lys213, Lys238, Lys254, Lys261, Lys272, Lys304, Lys319, Lys337, Lys340, Lys366, Lys377, Lys400, Lys407, Lys434, Lys469, Lys750, Lys770, Lys774, and Lys779
- NX_Q13263 (NX_Q13263-1)
- Modification sites at PhosphoSitePlus
Other Protein References for TRIM28 Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibodies for research
- LifeSpan BioSciences TRIM28 / KAP1 Antibody and TRIM28 / KAP1 Monoclonal Antibody
- G Biosciences Immunotag™ TIF1-ß Antibody
- NSJ Bioreagents TRIM28 Antibody / KAP1
- Novus Biologicals Antibodies for TRIM28
-
Abcam antibodies for TRIM28
- Invitrogen Antibodies for TRIM28 (AFLGC-TRIM28)
- antibodies-online: Show 58 available TRIM28 Antibodies ranked by validation data
- Compare Top TRIM28 Antibodies
-
Recommended
- 185 WB Antibodies (anti-human)
- 24 IF (p) Antibodies (anti-human)
- 4 IHC Antibodies (anti-human)
- 2 BCA Antibodies (anti-human)
- 2 IHC (p) Antibodies (anti-human)
- 2 RIA Antibodies (anti-human)
- 1 FACS Antibody (anti-human)
- 1 IF Antibody (anti-human)
- 1 ICFC Antibody (anti-human)
- 77 WB Antibodies (anti-mouse)
- 24 IF (p) Antibodies (anti-mouse)
- 1 IF Antibody (anti-mouse)
- 1 ICFC Antibody (anti-mouse)
- 53 WB Antibodies (anti-rat)
- 24 IF (p) Antibodies (anti-rat)
-
Custom Antibody ServicesProSci Antibodies for TRIM28
-
Santa Cruz Biotechnology (SCBT) Antibodies for TRIM28
- Sino Biological Antibodies for TRIM28
Protein products for research
- Search Origene for MassSpec and Protein Over-expression Lysates for TRIM28
- Origene Custom Protein Services for TRIM28
- ProSpec Recombinant Proteins for TRIM28
-
Abcam proteins for TRIM28
Assay products for research
No data available for DME Specific Peptides for TRIM28 Gene
Domains & Families for TRIM28 Gene
Gene Families for TRIM28 Gene
- HGNC:
- IUPHAR :
- Human Protein Atlas (HPA):
-
- Enzymes
- Plasma proteins
- Predicted intracellular proteins
Protein Domains for TRIM28 Gene
- InterPro:
- Blocks:
-
- Zn-finger, RING
- Zn-finger-like, PHD finger
- B-box zinc finger signature
- B-box, C-terminal
Suggested Antigen Peptide Sequences for TRIM28 Gene
- GenScript: Design optimal peptide antigens:
-
- Tripartite motif-containing protein 28 (TIF1B_HUMAN)
Graphical View of Domain Structure for InterPro Entry
Q13263UniProtKB/Swiss-Prot:
TIF1B_HUMAN :- The HP1 box is both necessary and sufficient for HP1 binding.
- Belongs to the TRIM/RBCC family.
- Domain:
-
- The HP1 box is both necessary and sufficient for HP1 binding.
- The PHD-type zinc finger enhances CEBPB transcriptional activity. The PHD-type zinc finger, the HP1 box and the bromo domain, function together to assemble the machinery required for repression of KRAB domain-containing proteins. Acts as an intramolecular SUMO E3 ligase for autosumoylation of bromodomain.
- The RING-finger-B Box-coiled-coil/tripartite motif (RBCC/TRIM motif) is required for interaction with the KRAB domain of KRAB-zinc finger proteins. Binds four zinc ions per molecule. The RING finger and the N-terminal of the leucine zipper alpha helical coiled-coil region of RBCC are required for oligomerization.
- Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
- Family:
-
- Belongs to the TRIM/RBCC family.
Function for TRIM28 Gene
Molecular function for TRIM28 Gene
- UniProtKB/Swiss-Prot Function:
- Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). In ESCs, in collaboration with SETDB1, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610). Acts as a corepressor for ZFP568 (By similarity).
- UniProtKB/Swiss-Prot Function:
- (Microbial infection) Plays a critical role in the shutdown of lytic gene expression during the early stage of herpes virus 8 primary infection. This inhibition is mediated through interaction with herpes virus 8 protein LANA1.
- UniProtKB/Swiss-Prot CatalyticActivity:
- Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27;.
Enzyme Numbers (IUBMB) for TRIM28 Gene
Phenotypes From GWAS Catalog for TRIM28 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0003677 | DNA binding | IBA,IDA | 9016654 |
| GO:0003682 | chromatin binding | IDA | 27029610 |
| GO:0003713 | transcription coactivator activity | ISS | -- |
| GO:0003714 | transcription corepressor activity | IEA,IDA | 8769649 |
| GO:0003723 | RNA binding | HDA | 22681889 |
Phenotypes for TRIM28 Gene
- MGI mutant phenotypes for TRIM28:
- inferred from 6 alleles
- GenomeRNAi human phenotypes for TRIM28:
-
- Decreased NF-kappaB reporter expression
- Decreased number of cells in monopolar arrest with EMD534085 (a Kinesin-5 inhibitor)
- shRNA abundance <= 50%
- no effect
- Decreased viability ratio
- Decreased viability after Maraba virus infection
- Decreased hepcidin::fluc mRNA expression
- Decreased ionizing radiation sensitivity
- Wnt reporter downregulated
- Decreased vesicular stomatitis virus (VSV) infection
- Decreased human cytomegalovirus (HCMV) strain AD169 replication
- Decreased viability
- Decreased influenza A virus infection
- Increased cell death in HCC-1954 cells
- Increased simian virus 40 (SV40) infection
- Decreased focal adhesion (FA) area, decreased FA length, decreased FA mean intensity, increased number of small and round FAs, decreased FA abundance, increased peripheral FA formation, decreased cell spreading
Animal Models for TRIM28 Gene
- MGI Knock Outs for TRIM28:
-
- Trim28 Trim28<tm1.2Ipc>
Animal Models for research
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
CRISPR products for research
- Applied Biological Materials (abm): CRISPR Clones for TRIM28 - Starting at $85 >
- * TRIM28 CRISPR as ready-to-use vector or virus: Non-viral | Lenti- | Adeno- | AAV- | siRNA - Browse All
- * Cas9 Nucleases, Nickases, Null Mutants, and GFP tag also available.
-
OriGene CRISPR knockouts for TRIM28
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for TRIM28
-
Santa Cruz Biotechnology (SCBT) CRISPR for TRIM28
miRNA for TRIM28 Gene
- miRTarBase miRNAs that target TRIM28
-
- hsa-miR-877-3p (MIRT037068)
- hsa-miR-744-5p (MIRT037599)
- hsa-miR-505-5p (MIRT037943)
- hsa-miR-423-5p (MIRT038141)
- hsa-miR-93-3p (MIRT038878)
- hsa-miR-505-3p (MIRT041001)
- hsa-miR-193b-3p (MIRT041271)
- hsa-miR-484 (MIRT041711)
- hsa-miR-149-5p (MIRT045571)
- hsa-miR-221-3p (MIRT046915)
- hsa-miR-183-5p (MIRT047101)
- hsa-miR-92a-3p (MIRT049122)
- hsa-miR-30a-5p (MIRT049953)
- hsa-miR-15a-5p (MIRT051347)
- hsa-let-7b-5p (MIRT052068)
- hsa-miR-6819-5p (MIRT465266)
- hsa-miR-6812-5p (MIRT465267)
- hsa-miR-6737-5p (MIRT465268)
- hsa-miR-6857-5p (MIRT465269)
- hsa-miR-491-5p (MIRT465270)
- hsa-miR-542-3p (MIRT465271)
- hsa-miR-6747-5p (MIRT465272)
- hsa-miR-608 (MIRT465273)
- hsa-miR-4651 (MIRT465274)
- hsa-miR-4664-5p (MIRT465275)
- hsa-miR-342-5p (MIRT465276)
- hsa-miR-4450 (MIRT465277)
- hsa-miR-4745-5p (MIRT465278)
- hsa-miR-4481 (MIRT465279)
- hsa-miR-6890-5p (MIRT465280)
- hsa-miR-4733-3p (MIRT465281)
- hsa-miR-6753-5p (MIRT465282)
- hsa-miR-1296-3p (MIRT465283)
- hsa-miR-4700-3p (MIRT465284)
- hsa-miR-1233-5p (MIRT736274)
- hsa-miR-1587 (MIRT737922)
- hsa-miR-1972 (MIRT738684)
- hsa-miR-3190-5p (MIRT740974)
- hsa-miR-3620-5p (MIRT741750)
- hsa-miR-378g (MIRT742770)
- hsa-miR-3919 (MIRT742965)
- hsa-miR-3925-5p (MIRT743009)
- hsa-miR-4270 (MIRT743605)
- hsa-miR-4436a (MIRT744445)
- hsa-miR-4441 (MIRT744572)
- hsa-miR-4492 (MIRT745304)
- hsa-miR-4498 (MIRT745385)
- hsa-miR-4505 (MIRT745545)
- hsa-miR-4650-5p (MIRT746526)
- hsa-miR-4656 (MIRT746660)
- hsa-miR-4675 (MIRT746891)
- hsa-miR-4717-5p (MIRT747527)
- hsa-miR-4741 (MIRT748128)
- hsa-miR-4755-3p (MIRT748375)
- hsa-miR-4773 (MIRT748701)
- hsa-miR-5000-3p (MIRT749332)
- hsa-miR-5001-5p (MIRT749378)
- hsa-miR-5702 (MIRT751344)
- hsa-miR-5787 (MIRT751494)
- hsa-miR-6754-5p (MIRT754747)
- hsa-miR-6777-5p (MIRT755399)
- hsa-miR-6778-5p (MIRT755469)
- hsa-miR-6829-5p (MIRT757117)
- hsa-miR-7113-5p (MIRT759128)
- hsa-miR-762 (MIRT759520)
miRNA products for research
Inhibitory RNAs for research
- Origene shrna, sirna, and RNAi products in human, mouse, rat for TRIM28
- Browse OriGene Inhibitory RNA Products For TRIM28
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for TRIM28
Clone products for research
- Applied Biological Materials (abm): Clones for TRIM28 - Starting at $45 >
- * TRIM28 as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Retro- | Protein Vector - Browse All
- * Viral Packaging Services and High Titer Production Services also available.
- Sino Biological Human cDNA Clone for TRIM28
- Addgene plasmids for TRIM28
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for TRIM28
Cell Lines for research
-
Horizon Cell Lines for TRIM28
No data available for Transcription Factor Targets and HOMER Transcription for TRIM28 Gene
Localization for TRIM28 Gene
Subcellular locations from UniProtKB/Swiss-Prot for TRIM28 Gene
- Nucleus. Note=Associated with centromeric heterochromatin during cell differentiation through CBX1. {ECO:0000250}.
| Compartment | Confidence |
|---|---|
| nucleus | 5 |
| plasma membrane | 2 |
| extracellular | 2 |
| cytoskeleton | 2 |
| mitochondrion | 2 |
| cytosol | 2 |
| peroxisome | 1 |
| endoplasmic reticulum | 1 |
| endosome | 1 |
| lysosome | 1 |
| golgi apparatus | 1 |
- Nucleoplasm (4)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000785 | chromatin | IBA | 21873635 |
| GO:0000791 | euchromatin | IEA | -- |
| GO:0000792 | heterochromatin | IEA | -- |
| GO:0005634 | nucleus | ISS | -- |
| GO:0005654 | nucleoplasm | IDA,TAS | -- |
Pathways & Interactions for TRIM28 Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Gene Expression |
.48
|
|
| 2 | Chromatin Regulation / Acetylation | ||
| 3 | DNA Damage | ||
| 4 | ATM Signaling Network in Development and Disease | ||
| 5 | C-MYB transcription factor network | ||
Pathways by source for TRIM28 Gene
6 BioSystems pathways for TRIM28 Gene
2 Reactome pathways for TRIM28 Gene
2 Cell Signaling Technology pathways for TRIM28 Gene
UniProtKB/Swiss-Prot Q13263-TIF1B_HUMAN
- Pathway: Protein modification; protein sumoylation.
Interacting Proteins for TRIM28 Gene
Selected Interacting proteins:
Q13263-TIF1B_HUMAN
ENSP00000253024
for TRIM28 Gene via
UniProtKB
MINT
STRING
IID
GPS-Prot Interaction Network for TRIM28
SIGNOR curated interactions for TRIM28 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000122 | negative regulation of transcription by RNA polymerase II | IEA | -- |
| GO:0001701 | in utero embryonic development | IEA | -- |
| GO:0001837 | epithelial to mesenchymal transition | ISS | -- |
| GO:0006281 | DNA repair | IDA | 17178852 |
| GO:0006325 | chromatin organization | IEA | -- |
Transcripts for TRIM28 Gene
mRNA/cDNA for TRIM28 Gene
- 1 REFSEQ mRNAs :
- 13 NCBI additional mRNA sequence :
- 15 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :
-
- ENST00000253024 3364 nts (view in UCSC)
- ENST00000341753 2709 nts (view in UCSC)
- ENST00000593582 533 nts (view in UCSC)
- ENST00000594806 861 nts (view in UCSC)
- ENST00000595028 486 nts (view in UCSC)
CRISPR products for research
- Applied Biological Materials (abm): CRISPR Clones for TRIM28 - Starting at $85 >
- * TRIM28 CRISPR as ready-to-use vector or virus: Non-viral | Lenti- | Adeno- | AAV- | siRNA - Browse All
- * Cas9 Nucleases, Nickases, Null Mutants, and GFP tag also available.
-
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- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for TRIM28
-
Santa Cruz Biotechnology (SCBT) CRISPR for TRIM28
miRNA products for research
Inhibitory RNAs for research
- Origene shrna, sirna, and RNAi products in human, mouse, rat for TRIM28
- Browse OriGene Inhibitory RNA Products For TRIM28
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for TRIM28
Clone products for research
- Applied Biological Materials (abm): Clones for TRIM28 - Starting at $45 >
- * TRIM28 as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Retro- | Protein Vector - Browse All
- * Viral Packaging Services and High Titer Production Services also available.
- Sino Biological Human cDNA Clone for TRIM28
- Addgene plasmids for TRIM28
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for TRIM28
| ExUns: | 1 | ^ | 2 | ^ | 3a | · | 3b | ^ | 4a | · | 4b | ^ | 5a | · | 5b | ^ | 6a | · | 6b | · | 6c | ^ | 7a | · | 7b | · | 7c | · | 7d | ^ | 8a | · | 8b | · | 8c | ^ | 9a | · | 9b | ^ | 10 | ^ | 11a | · | 11b | ^ | 12 | ^ | 13a | · | 13b | ^ |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | - | - | - | - | - | |||||||||||||||||||||||||||||||||||||||||||||||
| SP2: | - | - | - | - | - | - | - | - | ||||||||||||||||||||||||||||||||||||||||||||
| SP3: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||
| SP4: | - | - | - | - | ||||||||||||||||||||||||||||||||||||||||||||||||
| SP5: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP6: | - | - | - | - | - | - | - | |||||||||||||||||||||||||||||||||||||||||||||
| SP7: | - | - | - | - | ||||||||||||||||||||||||||||||||||||||||||||||||
| SP8: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||
| SP9: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP10: | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | ||||||||||||||||||||||||||||||||||||
| SP11: | - | - | - | |||||||||||||||||||||||||||||||||||||||||||||||||
| SP12: | - | - | ||||||||||||||||||||||||||||||||||||||||||||||||||
| SP13: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP14: | ||||||||||||||||||||||||||||||||||||||||||||||||||||
| SP15: |
| ExUns: | 14a | · | 14b | ^ | 15 | ^ | 16 | ^ | 17 | ^ | 18 | ^ | 19a | · | 19b | ^ | 20a | · | 20b | · | 20c |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | |||||||||||||||||||||
| SP2: | |||||||||||||||||||||
| SP3: | |||||||||||||||||||||
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| SP7: | |||||||||||||||||||||
| SP8: | |||||||||||||||||||||
| SP9: | |||||||||||||||||||||
| SP10: | - | - | - | - | - | - | - | ||||||||||||||
| SP11: | |||||||||||||||||||||
| SP12: | |||||||||||||||||||||
| SP13: | |||||||||||||||||||||
| SP14: | |||||||||||||||||||||
| SP15: |
Relevant External Links for TRIM28 Gene
- GeneLoc Exon Structure for
- TRIM28
Expression for TRIM28 Gene
This gene is overexpressed in Lymph node (9.3).
Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for TRIM28 Gene
- Elite partner
Transcriptomic regulation report from SPP (The Signaling Pathways Project) for TRIM28
SOURCE GeneReport for Unigene cluster for TRIM28 Gene:
Hs.467408mRNA Expression by UniProt/SwissProt for TRIM28 Gene:
Q13263-TIF1B_HUMAN
Tissue specificity:
Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Evidence on tissue expression from TISSUES for TRIM28 Gene
- Skin(4.9)
- Nervous system(4.7)
- Blood(4.7)
- Liver(4.7)
- Spleen(4.5)
- Lung(4.4)
- Intestine(4)
- Eye(3.8)
- Pancreas(3.7)
- Kidney(3.2)
- Muscle(3.1)
- Heart(3)
- Bone marrow(2.8)
- Lymph node(2.7)
- Thyroid gland(2.4)
- Stomach(2.3)
- Adrenal gland(2.1)
Primer products for research
-
OriGene qPCR primer pairs for TRIM28
-
OriGene qPCR primer pairs and template standards for TRIM28
Gene Expression Assays for research
No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for TRIM28 Gene
Orthologs for TRIM28 Gene
This gene was present in the common ancestor of animals.
| Organism | Taxonomy | Gene | Similarity | Type | Details |
|---|---|---|---|---|---|
| Chimpanzee (Pan troglodytes) |
Mammalia | TRIM28 30 |
|
OneToOne | |
| Dog (Canis familiaris) |
Mammalia | TRIM28 29 30 |
|
OneToOne | |
| Cow (Bos Taurus) |
Mammalia | TRIM28 29 30 |
|
OneToOne | |
| Rat (Rattus norvegicus) |
Mammalia | Trim28 29 |
|
||
| Mouse (Mus musculus) |
Mammalia | Trim28 29 16 30 |
|
OneToOne | |
| Platypus (Ornithorhynchus anatinus) |
Mammalia | TRIM28 30 |
|
OneToOne | |
| Chicken (Gallus gallus) |
Aves | -- 30 |
|
OneToMany | |
| -- 30 |
|
OneToMany | |||
| Lizard (Anolis carolinensis) |
Reptilia | TRIM28 30 |
|
OneToOne | |
| Tropical Clawed Frog (Silurana tropicalis) |
Amphibia | LOC100489687 29 |
|
||
| Str.3172 29 |
|
||||
| African clawed frog (Xenopus laevis) |
Amphibia | Xl.18843 29 |
|
||
| Zebrafish (Danio rerio) |
Actinopterygii | trim33l 30 |
|
OneToOne | |
| Fruit Fly (Drosophila melanogaster) |
Insecta | bon 31 |
|
|
|
| mei-P26 30 |
|
OneToMany | |||
| Worm (Caenorhabditis elegans) |
Secernentea | nhl-2 30 |
|
OneToMany | |
| Sea Squirt (Ciona savignyi) |
Ascidiacea | CSA.9380 30 |
|
OneToMany |
- Species where no ortholog for TRIM28 was found in the sources mined by GeneCards:
-
- A. gosspyii yeast (Eremothecium gossypii)
- Actinobacteria (Mycobacterium tuberculosis)
- African malaria mosquito (Anopheles gambiae)
- Alicante grape (Vitis vinifera)
- Alpha proteobacteria (Wolbachia pipientis)
- Amoeba (Dictyostelium discoideum)
- Archea (Pyrococcus horikoshii)
- Baker's yeast (Saccharomyces cerevisiae)
- Barley (Hordeum vulgare)
- Beta proteobacteria (Neisseria meningitidis)
- Bread mold (Neurospora crassa)
- Chromalveolata (Phytophthora infestans)
- Common water flea (Daphnia pulex)
- Corn (Zea mays)
- E. coli (Escherichia coli)
- Filamentous fungi (Aspergillus nidulans)
- Firmicute Bacteria (Streptococcus pneumoniae)
- Fission Yeast (Schizosaccharomyces pombe)
- Green Algae (Chlamydomonas reinhardtii)
- Honey Bee (Apis mellifera)
- K. Lactis Yeast (Kluyveromyces lactis)
- Loblloly Pine (Pinus taeda)
- Malaria Parasite (Plasmodium falciparum)
- Medicago Trunc (Medicago Truncatula)
- Moss (Physcomitrella patens)
- Oppossum (Monodelphis domestica)
- Orangutan (Pongo pygmaeus)
- Pig (Sus scrofa)
- Rainbow Trout (Oncorhynchus mykiss)
- Rice (Oryza sativa)
- Rice Blast Fungus (Magnaporthe grisea)
- Schistosome Parasite (Schistosoma mansoni)
- Sea Anemone (Nematostella vectensis)
- Sea Vase (Ciona intestinalis)
- Sea Urchin (Strongylocentrotus purpuratus)
- Sorghum (Sorghum bicolor)
- Soybean (Glycine max)
- Stem Rust Fungus (Puccinia graminis)
- Sugarcane (Saccharum officinarum)
- Thale Cress (Arabidopsis thaliana)
- Tomato (Lycopersicon esculentum)
- Toxoplasmosis (Toxoplasma gondii)
- Trichoplax (Trichoplax adhaerens)
- Wheat (Triticum aestivum)
Evolution for TRIM28 Gene
- ENSEMBL:
- Gene Tree for TRIM28 (if available)
- TreeFam:
- Gene Tree for TRIM28 (if available)
- Aminode:
- Evolutionary constrained regions (ECRs) for TRIM28: view image
- Alliance of Genome Resources:
- Additional Orthologs for TRIM28
Paralogs for TRIM28 Gene
Paralogs for TRIM28 Gene
- TRIM33 5
- TRIM24 5
- TRIM66 5
- PML 5
- TRIM55 5
- TRIM27 5
- TRIM26 5
- TRIM21 5
- TRIM68 5
- TRIM4 5
- TRIM7 5
- TRIM11 5
- TRIM38 5
- TRIM10 5
- TRIM15 5
- TRIM39 5
- TRIM35 5
- TRIM54 5
- TRIM50 5
- TRIM69 5
- TRIM62 5
- TRIM60 5
- TRIM41 5
- TRIM37 5
- TRIM46 5
- TRIM58 5
- MID1 5
- TRIM39-RPP21 5
- TRIM6 5
- TRIM6-TRIM34 5
- TRIM34 5
- TRIM5 5
- TRIM22 5
- MEFV 5
- TRIM71 5
- TRIM14 5
- MID2 5
- TRIM9 5
- TRIM63 5
- TRIM51GP 5
- TRIM51 5
- TRIM72 5
- TRIM56 5
- TRIM75P 5
- TRIM77 5
- TRIM42 5
- TRIML1 5
- TRIM25 5
- TRIM45 5
- TRIM17 5
- TRIM67 5
- TRIM13 5
- TRIM31 5
- BSPRY 5
- TRIM32 5
- TRIM16 5
- TRIM49B 5
- TRIM64C 5
- TRIM36 5
- TRIM8 5
- TRIM43B 5
- TRIM43 5
- TRIM2 5
- ENSG00000288637 5
- TRIM61 5
- TRIM49 5
- TRIM64B 5
- TRIM49D1 5
- TRIM49D2 5
- TRIM64 5
- TRIM49C 5
- TRIML2 5
- TRIM29 5
- TRIM47 5
- TRIM65 5
- TRIM40 5
- TRIM3 5
- TRIM74 5
- TRIM44 5
- TRIM73 5
- RNF39 5
- TRIM48 5
- TRIM52 5
- ENSG00000239920 5
Pseudogenes.org Pseudogenes for TRIM28 Gene
Variants for TRIM28 Gene
Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for TRIM28 Gene
| SNP ID | Clinical significance and condition | Chr 19 pos | Variation | AA Info | Type |
|---|---|---|---|---|---|
| 973190 | Pathogenic: Nephroblastoma | 58,549,855(+) |
C/T NM_005762.3(TRIM28):c.2101C>T (p.Gln701Ter) |
NONSENSE | |
| rs147302539 | Likely Benign: not provided | 58,548,075(+) |
C/T NM_005762.3(TRIM28):c.996C>T (p.His332=) |
SYNONYMOUS | |
| rs193921106 | Uncertain Significance: Malignant tumor of prostate | 58,550,038(+) |
G/T NM_005762.3(TRIM28):c.2193+3G>T |
INTRON | |
| rs398122526 | Not Provided: Familial colorectal cancer | 58,548,431(+) |
C/T NM_005762.3(TRIM28):c.1216+23C>T |
INTRON | |
| rs75494560 | Benign: not provided | 58,545,438(+) |
C/T NM_005762.3(TRIM28):c.354C>T (p.Pro118=) |
SYNONYMOUS |
All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).
| Variant ID | Type | Subtype | PubMed ID |
|---|---|---|---|
| dgv3687n100 | CNV | gain | 25217958 |
| dgv3688n100 | CNV | gain | 25217958 |
| nsv1057077 | CNV | gain | 25217958 |
| nsv1057206 | CNV | gain | 25217958 |
| nsv1144885 | CNV | deletion | 24896259 |
| nsv1160670 | CNV | deletion | 26073780 |
| nsv515731 | CNV | gain+loss | 19592680 |
| nsv828657 | CNV | gain | 20364138 |
| nsv952156 | CNV | deletion | 24416366 |
Residual Variation Intolerance Score:
4.17% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score:
1.10;
22.43% of all genes are more intolerant (likely to be disease-causing)
Additional Variant Information for TRIM28 Gene
SNP Genotyping and Copy Number Assays for research
No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for TRIM28 Gene
Disorders for TRIM28 Gene
| Disorder | Aliases | PubMed IDs |
|---|---|---|
| wilms tumor 1 |
|
|
| wilms tumor 5 |
|
|
| hyperoxaluria, primary, type i |
|
|
| breast medullary carcinoma |
|
|
| colorectal cancer |
|
|
Search TRIM28 in MalaCards
View complete list of genes associated with diseases
Additional Disease Information for TRIM28
- Atlas
- ATLAS of Genetics and Cytogenetics in Oncology and Haematology
- Open Targets Platform
- Open Targets Platform
No data available for UniProtKB/Swiss-Prot and Genatlas for TRIM28 Gene
Publications for TRIM28 Gene
- Solution structure of the PHD domain from the KAP-1 corepressor: structural determinants for PHD, RING and LIM zinc-binding domains. (PMID: 11226167) Capili AD … Borden KL (The EMBO journal 2001) 2 3 4 22
- SUMOylation of the transcriptional co-repressor KAP1 is regulated by the serine and threonine phosphatase PP1. (PMID: 20424263) Li X … Ann DK (Science signaling 2010) 3 4 22
- A novel GDNF-inducible gene, BMZF3, encodes a transcriptional repressor associated with KAP-1. (PMID: 18060868) Suzuki C … Takahashi M (Biochemical and biophysical research communications 2008) 3 4 22
- Structural insights into human KAP1 PHD finger-bromodomain and its role in gene silencing. (PMID: 18488044) Zeng L … Zhou MM (Nature structural & molecular biology 2008) 3 4 22
- Regulation of E2F1 function by the nuclear corepressor KAP1. (PMID: 17704056) Wang C … Chen J (The Journal of biological chemistry 2007) 3 4 22
ExternalLinks
Products for TRIM28 Gene
- Custom Antibody ServicesOriGene Antibodies for TRIM28
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- Custom Assay Services
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- Origene Custom Protein Services for TRIM28
- Origene shrna, sirna, and RNAi products in human, mouse, rat for TRIM28
- Browse OriGene Inhibitory RNA Products For TRIM28
- OriGene qPCR primer pairs for TRIM28
- OriGene qPCR primer pairs and template standards for TRIM28
- OriGene CRISPR knockouts for TRIM28
- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Browse OriGene miRNA Products For TRIM28
- Sino Biological Human cDNA Clone for TRIM28
- Browse Sino Biological Recombinant Proteins
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- Sino Biological Antibodies for TRIM28
- Browse Sino Biological Assays
- Browse Antibodies
- Browse cDNA Clones
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- Browse CRO Services
- High-throughput Antibody Production Service
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- Novus Biologicals proteins and lysates for TRIM28
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