The protein encoded by this gene is a tripartite motif (TRIM) family member that contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. While it lacks a RING domain found in other TRIM proteins, the encoded protein can homodimerize or heterodimerize with other TRIM proteins and has E3 ubiquitin ligase activity. Thi... See more...

Aliases for TRIM16 Gene

Aliases for TRIM16 Gene

  • Tripartite Motif Containing 16 2 3 5
  • Estrogen-Responsive B Box Protein 2 3 4
  • Tripartite Motif-Containing Protein 16 3 4
  • E3 Ubiquitin-Protein Ligase TRIM16 3 4
  • EBBP 3 4
  • Tripartite Motif-Containing 16 2
  • EC 2.3.2.27 4

External Ids for TRIM16 Gene

Previous GeneCards Identifiers for TRIM16 Gene

  • GC17M015897
  • GC17M016646
  • GC17M015474
  • GC17M015731
  • GC17M015472
  • GC17M015531
  • GC17M015404

Summaries for TRIM16 Gene

Entrez Gene Summary for TRIM16 Gene

  • The protein encoded by this gene is a tripartite motif (TRIM) family member that contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. While it lacks a RING domain found in other TRIM proteins, the encoded protein can homodimerize or heterodimerize with other TRIM proteins and has E3 ubiquitin ligase activity. This gene is also a tumor suppressor and is involved in secretory autophagy. [provided by RefSeq, Jan 2017]

GeneCards Summary for TRIM16 Gene

TRIM16 (Tripartite Motif Containing 16) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and NACHT domain binding. An important paralog of this gene is TRIM16L.

UniProtKB/Swiss-Prot Summary for TRIM16 Gene

  • E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.

Gene Wiki entry for TRIM16 Gene

Additional gene information for TRIM16 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for TRIM16 Gene

Genomics for TRIM16 Gene

GeneHancer (GH) Regulatory Elements for TRIM16 Gene

Promoters and enhancers for TRIM16 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH17J015648 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 500.3 +33.9 33898 4.8 ZNF217 NRF1 USF1 JUND FOS CLOCK TRIM28 REST RFX1 MYC ENSG00000266261 ENSG00000251537 TRIM16 CDRT4 CDRT1 PIGL TBC1D26 MEIS3P1 piR-53431-279 lnc-TRIM16-1
GH17J015683 Promoter/Enhancer 1.5 Ensembl ENCODE CraniofacialAtlas 500.7 +0.4 411 2.2 ZBTB40 ZNF217 POLR2G SIX5 ZBTB11 GABPA POLR2A KMT2B HCFC1 SIN3A TRIM16 lnc-TRIM16-1 TBC1D26 ENSG00000265519 ZNF29P
GH17J015642 Promoter/Enhancer 1.2 EPDnew Ensembl dbSUPER 500.3 +41.5 41510 0.8 WT1 ZNF316 NFE2 MAFG GLIS1 MAFK PRDM10 ZBTB8A CBFA2T3 EMSY TRIM16 piR-53431-279 ENSG00000266261 ENSG00000251537 lnc-TRIM16-1
GH17J015661 Enhancer 0.4 Ensembl dbSUPER 5.6 +21.3 21272 1.3 FOS CDRT4 TRIM16 ENSG00000251537 ZNF29P lnc-TRIM16-1
GH17J014840 Enhancer 0.3 Ensembl ENCODE 6.3 +843.9 843941 1.9 ENSG00000205325 TRIM16 LINC02096 lnc-HS3ST3B1-13 RPL23AP76 lnc-PMP22-6
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around TRIM16 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for TRIM16

Top Transcription factor binding sites by QIAGEN in the TRIM16 gene promoter:
  • AREB6
  • Evi-1
  • FOXL1
  • MRF-2
  • Pbx1a
  • POU2F1
  • POU2F1a
  • PPAR-gamma1
  • PPAR-gamma2
  • STAT3

Genomic Locations for TRIM16 Gene

Genomic Locations for TRIM16 Gene
chr17:15,627,960-15,684,311
(GRCh38/hg38)
Size:
56,352 bases
Orientation:
Minus strand
chr17:15,531,274-15,587,625
(GRCh37/hg19)
Size:
56,352 bases
Orientation:
Minus strand

Genomic View for TRIM16 Gene

Genes around TRIM16 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
TRIM16 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for TRIM16 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for TRIM16 Gene

Proteins for TRIM16 Gene

  • Protein details for TRIM16 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O95361-TRI16_HUMAN
    Recommended name:
    Tripartite motif-containing protein 16
    Protein Accession:
    O95361
    Secondary Accessions:
    • Q6IAL8
    • Q7Z6I2
    • Q96BE8
    • Q96J43

    Protein attributes for TRIM16 Gene

    Size:
    564 amino acids
    Molecular mass:
    63955 Da
    Quaternary structure:
    • Homodimerizes via its coiled-coil domain. Heterodimerizes with MID1, TRIM24 and PML. Interacts with Galectin-3/LGALS3 in a ULK1-dependent manner; this interaction mediates autophagy of damage endomembranes. Interacts with BECN1. Interacts with ATG16L1. Interacts with p62/SQSTM and LC3B/MAP1LC3B.

    Alternative splice isoforms for TRIM16 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for TRIM16 Gene

Post-translational modifications for TRIM16 Gene

No data available for DME Specific Peptides for TRIM16 Gene

Domains & Families for TRIM16 Gene

Gene Families for TRIM16 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for TRIM16 Gene

Blocks:
  • B-box zinc finger signature
  • Butyrophylin C-terminal DUF signature
  • SPRY-associated domain
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for TRIM16 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ31464 fis, clone NT2NE2001337, highly similar to Tripartite motif-containing protein 16 (B3KP96_HUMAN)
  • Estrogen-responsive B box protein (TRI16_HUMAN)

Graphical View of Domain Structure for InterPro Entry

O95361

UniProtKB/Swiss-Prot:

TRI16_HUMAN :
  • Belongs to the TRIM/RBCC family.
Family:
  • Belongs to the TRIM/RBCC family.
genes like me logo Genes that share domains with TRIM16: view

Function for TRIM16 Gene

Molecular function for TRIM16 Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence=. ;.

Enzyme Numbers (IUBMB) for TRIM16 Gene

Phenotypes From GWAS Catalog for TRIM16 Gene

Gene Ontology (GO) - Molecular Function for TRIM16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IDA 16636064
GO:0005515 protein binding IPI,IEA 16575408
GO:0008270 zinc ion binding IEA --
GO:0016740 transferase activity IEA --
GO:0019966 interleukin-1 binding IPI 16575408
genes like me logo Genes that share ontologies with TRIM16: view
genes like me logo Genes that share phenotypes with TRIM16: view

Animal Model Products

CRISPR Products

miRNA for TRIM16 Gene

miRTarBase miRNAs that target TRIM16

Clone Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for TRIM16 Gene

Localization for TRIM16 Gene

Subcellular locations from UniProtKB/Swiss-Prot for TRIM16 Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for TRIM16 gene
Compartment Confidence
nucleus 5
plasma membrane 4
cytosol 4
extracellular 1
cytoskeleton 1
mitochondrion 1
endoplasmic reticulum 1
endosome 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (3)
  • Plasma membrane (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for TRIM16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA,IDA 9817599
GO:0005829 cytosol IDA --
GO:0005886 plasma membrane IDA --
GO:0016605 PML body IDA 16636064
genes like me logo Genes that share ontologies with TRIM16: view

Pathways & Interactions for TRIM16 Gene

PathCards logo

SuperPathways for TRIM16 Gene

No Data Available

Gene Ontology (GO) - Biological Process for TRIM16 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0032526 response to retinoic acid IEP 16636064
GO:0043966 histone H3 acetylation IDA 19147277
GO:0043967 histone H4 acetylation IDA 16636064
GO:0045618 positive regulation of keratinocyte differentiation IDA 11919186
genes like me logo Genes that share ontologies with TRIM16: view

No data available for Pathways by source and SIGNOR curated interactions for TRIM16 Gene

Drugs & Compounds for TRIM16 Gene

No Compound Related Data Available

Transcripts for TRIM16 Gene

mRNA/cDNA for TRIM16 Gene

8 REFSEQ mRNAs :
10 NCBI additional mRNA sequence :
26 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for TRIM16 Gene

No ASD Table

Relevant External Links for TRIM16 Gene

GeneLoc Exon Structure for
TRIM16

Expression for TRIM16 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for TRIM16 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for TRIM16 Gene

This gene is overexpressed in Cervix (51.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for TRIM16 Gene



Transcriptomic regulation report from SPP (The Signaling Pathways Project) for TRIM16

SOURCE GeneReport for Unigene cluster for TRIM16 Gene:

Hs.123534

mRNA Expression by UniProt/SwissProt for TRIM16 Gene:

O95361-TRI16_HUMAN
Tissue specificity: Highest levels found in testis, ovary, small intestine, colon, placenta, heart, skeletal muscle and mammary gland. More highly expressed in the fetus than in the corresponding adult tissues. Expressed in basal keratinocytes.

Evidence on tissue expression from TISSUES for TRIM16 Gene

  • Skin(4.6)
  • Lung(3.1)
  • Nervous system(3)
genes like me logo Genes that share expression patterns with TRIM16: view

No data available for mRNA differential expression in normal tissues , Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for TRIM16 Gene

Orthologs for TRIM16 Gene

This gene was present in the common ancestor of chordates.

Orthologs for TRIM16 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia TRIM16 31 30
  • 97.16 (n)
OneToOne
dog
(Canis familiaris)
Mammalia LOC489518 30
  • 84.34 (n)
mouse
(Mus musculus)
Mammalia Trim16 17 31 30
  • 81.2 (n)
rat
(Rattus norvegicus)
Mammalia Trim16 30
  • 80.9 (n)
cow
(Bos Taurus)
Mammalia TRIM16 30
  • 79.52 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 67 (a)
OneToMany
oppossum
(Monodelphis domestica)
Mammalia -- 31
  • 60 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia -- 31
  • 49 (a)
OneToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia LOC100490556 30
  • 61.98 (n)
zebrafish
(Danio rerio)
Actinopterygii trim16 31 30
  • 55.78 (n)
OneToMany
Species where no ortholog for TRIM16 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for TRIM16 Gene

ENSEMBL:
Gene Tree for TRIM16 (if available)
TreeFam:
Gene Tree for TRIM16 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for TRIM16: view image

Paralogs for TRIM16 Gene

(1) SIMAP similar genes for TRIM16 Gene using alignment to 9 proteins:

  • TRI16_HUMAN
  • B3KP96_HUMAN
  • J3QKY5_HUMAN
  • J3QL38_HUMAN
  • J3QLP0_HUMAN
  • J3QR69_HUMAN
  • K7EJH2_HUMAN
  • K7EL43_HUMAN
  • K7ENN8_HUMAN
genes like me logo Genes that share paralogs with TRIM16: view

Variants for TRIM16 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for TRIM16 Gene

SNP ID Clinical significance and condition Chr 17 pos Variation AA Info Type
770442 Benign: not provided 15,628,628(-) C/A MISSENSE_VARIANT
rs1060903 - p.Gly561Val
rs2074890 - p.Glu121Asp
rs3174720 - p.Arg493Trp

Additional dbSNP identifiers (rs#s) for TRIM16 Gene

Structural Variations from Database of Genomic Variants (DGV) for TRIM16 Gene

Variant ID Type Subtype PubMed ID
dgv890e212 CNV loss 25503493
esv2421995 CNV deletion 20811451
esv3432585 CNV insertion 20981092
esv3582544 CNV loss 25503493
esv3640078 CNV gain 21293372
esv3892964 CNV gain 25118596
nsv1067123 CNV loss 25217958
nsv1136229 CNV deletion 24896259
nsv1160454 CNV deletion 26073780
nsv1160455 CNV deletion 26073780
nsv442736 CNV loss 18776908
nsv457695 CNV gain 19166990
nsv518620 CNV loss 19592680
nsv520426 CNV loss 19592680
nsv522130 CNV loss 19592680
nsv574462 CNV gain 21841781
nsv953832 CNV deletion 24416366
nsv960058 CNV duplication 23825009
nsv962261 CNV duplication 23825009
nsv978368 CNV duplication 23825009
nsv978369 CNV duplication 23825009

Variation tolerance for TRIM16 Gene

Residual Variation Intolerance Score: 92.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.71; 66.20% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for TRIM16 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
TRIM16

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for TRIM16 Gene

Disorders for TRIM16 Gene

Additional Disease Information for TRIM16

No disorders were found for TRIM16 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for TRIM16 Gene

Publications for TRIM16 Gene

  1. The novel estrogen-responsive B-box protein (EBBP) gene is tamoxifen-regulated in cells expressing an estrogen receptor DNA-binding domain mutant. (PMID: 9817599) Liu HL … Zajchowski DA (Molecular endocrinology (Baltimore, Md.) 1998) 3 4 23 54
  2. TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis and autophagy. (PMID: 30143514) Jena KK … Chauhan S (The EMBO journal 2018) 3 4 54
  3. TRIMs and Galectins Globally Cooperate and TRIM16 and Galectin-3 Co-direct Autophagy in Endomembrane Damage Homeostasis. (PMID: 27693506) Chauhan S … Deretic V (Developmental cell 2016) 3 4 54
  4. TRIM16 acts as an E3 ubiquitin ligase and can heterodimerize with other TRIM family members. (PMID: 22629402) Bell JL … Cheung BB (PloS one 2012) 3 4 54
  5. The estrogen-responsive B box protein (EBBP) restores retinoid sensitivity in retinoid-resistant cancer cells via effects on histone acetylation. (PMID: 19147277) Raif A … Cheung BB (Cancer letters 2009) 3 23 54

Products for TRIM16 Gene

Sources for TRIM16 Gene