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Aliases for TLK2 Gene

Aliases for TLK2 Gene

  • Tousled Like Kinase 2 2 3 5
  • Tousled-Like Kinase 2 2 4
  • EC 2.7.11.1 4 56
  • PKU-ALPHA 3 4
  • HsHPK 3 4
  • Serine/Threonine-Protein Kinase Tousled-Like 2 3
  • EC 2.7.11 56

External Ids for TLK2 Gene

Previous GeneCards Identifiers for TLK2 Gene

  • GC17P060240
  • GC17M034403
  • GC17P060897
  • GC17P061029
  • GC17P061009
  • GC17P057889
  • GC17P060556
  • GC17P055922
  • GC17P060536

Summaries for TLK2 Gene

Entrez Gene Summary for TLK2 Gene

  • This gene encodes a nuclear serine/threonine kinase that was first identified in Arabidopsis. The encoded protein is thought to function in the regulation of chromatin assembly in the S phase of the cell cycle by regulating the levels of a histone H3/H4 chaperone. This protein is associated with double-strand break repair of DNA damage caused by radiation. Pseudogenes of this gene are present on chromosomes 10 and 17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

GeneCards Summary for TLK2 Gene

TLK2 (Tousled Like Kinase 2) is a Protein Coding gene. Among its related pathways are DNA Damage Response and Chks in Checkpoint Regulation. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is TLK1.

UniProtKB/Swiss-Prot for TLK2 Gene

  • Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy.

Gene Wiki entry for TLK2 Gene

Additional gene information for TLK2 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for TLK2 Gene

Genomics for TLK2 Gene

GeneHancer (GH) Regulatory Elements for TLK2 Gene

Promoters and enhancers for TLK2 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH17I062477 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE dbSUPER 566.2 +20.5 20510 3.7 SIN3A ZBTB7B ZNF48 GLIS2 ELK1 SP3 NFYC MXD4 REST GLIS1 TLK2 MED13 RPS10P26 MRC2 METTL2A INTS2
GH17I062459 Enhancer 0.4 Ensembl 550.8 +0.4 443 0.2 ZNF148 CEBPB TLK2 RPS10P26
GH17I063444 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE dbSUPER 15.9 +988.0 988033 4.3 HDGF CLOCK ARNT SIN3A DMAP1 ZBTB7B YY1 ZNF766 ZNF207 NFYC CYB561 FTSJ3 TLK2 SMARCD2 ENSG00000281311 DDX42 ENSG00000265971
GH17I062625 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE dbSUPER 16 +169.9 169863 5.9 SIN3A YBX1 GLIS2 FOS ZNF263 SP3 REST NFIL3 GMEB1 ZNF585B MRC2 ENSG00000274565 TLK2 INTS2 MED13 DCAF7 ENSG00000277463 GC17P062639
GH17I061926 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 15.9 -531.2 -531162 2.5 DMAP1 IRF4 YY1 SLC30A9 ZNF213 E2F8 ZNF143 ZNF548 SP3 SSRP1 INTS2 MED13 RPS10P26 TLK2 GC17M061875 ENSG00000202361
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around TLK2 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the TLK2 gene promoter:

Genomic Locations for TLK2 Gene

Genomic Locations for TLK2 Gene
chr17:62,458,658-62,615,481
(GRCh38/hg38)
Size:
156,824 bases
Orientation:
Plus strand
chr17:60,536,019-60,692,842
(GRCh37/hg19)

Genomic View for TLK2 Gene

Genes around TLK2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
TLK2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for TLK2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for TLK2 Gene

Proteins for TLK2 Gene

  • Protein details for TLK2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q86UE8-TLK2_HUMAN
    Recommended name:
    Serine/threonine-protein kinase tousled-like 2
    Protein Accession:
    Q86UE8
    Secondary Accessions:
    • D3DU07
    • Q9UKI7
    • Q9Y4F7

    Protein attributes for TLK2 Gene

    Size:
    772 amino acids
    Molecular mass:
    87661 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Monomer and heterodimer with TLK1. Interacts with ASF1A and ASF1B. Association with 14-3-3 proteins such as YWHAZ regulates subcellular location. May also interact with FEZ1/LZTS1 and FEZ2.
    SequenceCaution:
    • Sequence=AAF03095.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=AAH44925.2; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAA20561.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for TLK2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for TLK2 Gene

Selected DME Specific Peptides for TLK2 Gene

Q86UE8:
  • EKKENYHKHACREYRIHK
  • RYLLLHLLGRGGFSEVYKAFDL
  • VNALKYLNEI
  • LEKYKERLN
  • ELHSLDPRRQELLEARF
  • AGTYWYLPPE
  • IKITDFGL
  • KPGNILL
  • GNDLDFYLKQ
  • LDHPRIVKLYDYFSLDTD
  • DPYLLPHIRKSV
  • EQWTDGYAFQNL
  • AVKIHQLNK
  • SQFKDHPTLN
  • IKITDFG
  • CVTMSKKLLIEKSKQEK

Post-translational modifications for TLK2 Gene

Domains & Families for TLK2 Gene

Gene Families for TLK2 Gene

IUPHAR :
Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins

Suggested Antigen Peptide Sequences for TLK2 Gene

Graphical View of Domain Structure for InterPro Entry

Q86UE8

UniProtKB/Swiss-Prot:

TLK2_HUMAN :
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Family:
  • Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
genes like me logo Genes that share domains with TLK2: view

Function for TLK2 Gene

Molecular function for TLK2 Gene

GENATLAS Biochemistry:
tousled-like kinase 2,nuclear serine/threonine kinase PKU alpha
UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Cell cycle-regulated, with maximal activity in the S-phase. Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase, probably by CHEK1, possibly at Ser-750. This inhibition is cell cycle checkpoint- and ATM-dependent.
UniProtKB/Swiss-Prot Function:
Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy.

Enzyme Numbers (IUBMB) for TLK2 Gene

Phenotypes From GWAS Catalog for TLK2 Gene

Gene Ontology (GO) - Molecular Function for TLK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IEA --
GO:0004674 protein serine/threonine kinase activity IEA,IDA 12660173
GO:0005515 protein binding IPI 21903422
GO:0005524 ATP binding IEA,IDA 12660173
GO:0016301 kinase activity IEA --
genes like me logo Genes that share ontologies with TLK2: view
genes like me logo Genes that share phenotypes with TLK2: view

Animal Model Products

  • Taconic Biosciences Mouse Models for TLK2

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for TLK2

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for TLK2 Gene

Localization for TLK2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for TLK2 Gene

Nucleus. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Note=Colocalizes with the cytoplasmic intermediate filament system during the G1 phase of the cell cycle. Present in the perinuclear region at S phase and in the nucleus at late G2.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for TLK2 gene
Compartment Confidence
cytoskeleton 5
nucleus 5
cytosol 2

Gene Ontology (GO) - Cellular Components for TLK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA,IDA 9427565
GO:0005737 cytoplasm IEA --
GO:0005856 cytoskeleton IEA --
GO:0005882 intermediate filament IDA 10455159
GO:0048471 perinuclear region of cytoplasm IDA,IEA 10455159
genes like me logo Genes that share ontologies with TLK2: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for TLK2 Gene

Pathways & Interactions for TLK2 Gene

genes like me logo Genes that share pathways with TLK2: view

Pathways by source for TLK2 Gene

2 BioSystems pathways for TLK2 Gene
1 Qiagen pathway for TLK2 Gene

Gene Ontology (GO) - Biological Process for TLK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001672 regulation of chromatin assembly or disassembly IDA 12660173
GO:0006468 protein phosphorylation IEA,IDA 12660173
GO:0006974 cellular response to DNA damage stimulus IEA,IDA 12660173
GO:0007049 cell cycle IEA --
GO:0007059 chromosome segregation IMP 20705056
genes like me logo Genes that share ontologies with TLK2: view

No data available for SIGNOR curated interactions for TLK2 Gene

Drugs & Compounds for TLK2 Gene

(6) Drugs for TLK2 Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Magnesium Approved Nutra 0
ATP Investigational Nutra Agonist 0

(3) Additional Compounds for TLK2 Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
genes like me logo Genes that share compounds with TLK2: view

Transcripts for TLK2 Gene

Unigene Clusters for TLK2 Gene

Tousled-like kinase 2:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for TLK2

Alternative Splicing Database (ASD) splice patterns (SP) for TLK2 Gene

No ASD Table

Relevant External Links for TLK2 Gene

GeneLoc Exon Structure for
TLK2
ECgene alternative splicing isoforms for
TLK2

Expression for TLK2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for TLK2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for TLK2 Gene

This gene is overexpressed in CD8 Tcells (11.0), Peripheral blood mononuclear cells (9.3), Breast (8.6), Lymph node (8.2), Fetal ovary (7.9), and Testis (6.9).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for TLK2 Gene



Protein tissue co-expression partners for TLK2 Gene

NURSA nuclear receptor signaling pathways regulating expression of TLK2 Gene:

TLK2

SOURCE GeneReport for Unigene cluster for TLK2 Gene:

Hs.445078

mRNA Expression by UniProt/SwissProt for TLK2 Gene:

Q86UE8-TLK2_HUMAN
Tissue specificity: Ubiquitous. Detected in placenta, fetal liver, kidney, pancreas, heart and skeletal muscle. Highly expressed in testis. Detected in spleen, thymus, colon, ovary, small intestine, prostate and peripheral blood leukocytes.

Evidence on tissue expression from TISSUES for TLK2 Gene

  • Liver(4.3)
  • Nervous system(2.7)
genes like me logo Genes that share expression patterns with TLK2: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for TLK2 Gene

Orthologs for TLK2 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for TLK2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia TLK2 33 34
  • 99.91 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia -- 34
  • 96 (a)
OneToMany
-- 34
  • 80 (a)
OneToMany
cow
(Bos Taurus)
Mammalia TLK2 33 34
  • 95.91 (n)
dog
(Canis familiaris)
Mammalia TLK2 33 34
  • 95.6 (n)
mouse
(Mus musculus)
Mammalia Tlk2 33 16 34
  • 93.6 (n)
rat
(Rattus norvegicus)
Mammalia Tlk2 33
  • 93.56 (n)
oppossum
(Monodelphis domestica)
Mammalia TLK2 34
  • 89 (a)
OneToOne
chicken
(Gallus gallus)
Aves TLK2 33 34
  • 87.17 (n)
lizard
(Anolis carolinensis)
Reptilia TLK2 34
  • 96 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia tlk2 33
  • 80.65 (n)
zebrafish
(Danio rerio)
Actinopterygii tlk2 33 34
  • 74.06 (n)
Dr.7649 33
fruit fly
(Drosophila melanogaster)
Insecta BcDNA:GH07910 35
  • 75 (a)
tlk 33 34
  • 60.88 (n)
worm
(Caenorhabditis elegans)
Secernentea tlk-1 33 34
  • 54.08 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes MPS1 34
  • 16 (a)
OneToMany
corn
(Zea mays)
Liliopsida Zm.10347 33
sea squirt
(Ciona savignyi)
Ascidiacea CSA.566 34
  • 65 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.14850 33
Species where no ortholog for TLK2 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for TLK2 Gene

ENSEMBL:
Gene Tree for TLK2 (if available)
TreeFam:
Gene Tree for TLK2 (if available)

Paralogs for TLK2 Gene

Paralogs for TLK2 Gene

Pseudogenes.org Pseudogenes for TLK2 Gene

genes like me logo Genes that share paralogs with TLK2: view

Variants for TLK2 Gene

Sequence variations from dbSNP and Humsavar for TLK2 Gene

SNP ID Clin Chr 17 pos Variation AA Info Type
VAR_041221 A gastric adenocarcinoma sample p.Phe173Leu
rs1057524147 uncertain-significance, not specified 62,565,001(+) T/C coding_sequence_variant, missense_variant
rs1000073188 -- 62,604,719(+) G/A genic_downstream_transcript_variant, intron_variant
rs1000093135 -- 62,498,468(+) C/T intron_variant
rs1000149610 -- 62,492,618(+) G/A/C intron_variant

Structural Variations from Database of Genomic Variants (DGV) for TLK2 Gene

Variant ID Type Subtype PubMed ID
esv1421317 OTHER inversion 17803354
esv3640974 CNV gain 21293372
nsv1109100 CNV deletion 24896259
nsv1141308 CNV duplication 24896259
nsv2114 CNV insertion 18451855
nsv473798 CNV novel sequence insertion 20440878
nsv509664 CNV insertion 20534489
nsv516636 CNV loss 19592680
nsv952352 CNV duplication 24416366
nsv954596 CNV deletion 24416366
nsv960517 CNV duplication 23825009
nsv960518 CNV duplication 23825009
nsv962359 CNV duplication 23825009
nsv978451 CNV duplication 23825009

Variation tolerance for TLK2 Gene

Residual Variation Intolerance Score: 18.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.53; 11.44% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for TLK2 Gene

Human Gene Mutation Database (HGMD)
TLK2
SNPedia medical, phenotypic, and genealogical associations of SNPs for
TLK2

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for TLK2 Gene

Disorders for TLK2 Gene

Additional Disease Information for TLK2

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for TLK2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for TLK2 Gene

Publications for TLK2 Gene

  1. Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases. (PMID: 11470414) Silljé HH … Nigg EA (Current biology : CB 2001) 3 4 22 58
  2. Mammalian homologues of the plant Tousled gene code for cell-cycle-regulated kinases with maximal activities linked to ongoing DNA replication. (PMID: 10523312) Silljé HH … Nigg EA (The EMBO journal 1999) 2 3 4 58
  3. cDNA cloning and chromosomal mapping of genes encoding novel protein kinases termed PKU-alpha and PKU-beta, which have nuclear localization signal. (PMID: 9427565) Yamakawa A … Date T (Gene 1997) 2 3 4 58
  4. Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC. (PMID: 22354037) McKnight NC … Tooze SA (The EMBO journal 2012) 3 4 58
  5. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 44 58

Products for TLK2 Gene

  • Addgene plasmids for TLK2

Sources for TLK2 Gene

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