This gene encodes a component of the nuclear THO transcription elongation complex, which is part of the larger transcription export (TREX) complex that couples messenger RNA processing and export. In humans, the transcription export complex is recruited to the 5'-end of messenger RNAs in a splicing- and cap-dependent manner. Studies of a related complex in mouse suggest that th... See more...

Aliases for THOC3 Gene

Aliases for THOC3 Gene

  • THO Complex 3 2 3 5
  • THO Complex Subunit 3 3 4
  • TEX1 Homolog 3 4
  • HTREX45 3 4
  • THO3 3
  • Tho3 4

External Ids for THOC3 Gene

Previous GeneCards Identifiers for THOC3 Gene

  • GC05M176207
  • GC05M175280
  • GC05M175367
  • GC05M175319
  • GC05M175344
  • GC05P172063

Summaries for THOC3 Gene

Entrez Gene Summary for THOC3 Gene

  • This gene encodes a component of the nuclear THO transcription elongation complex, which is part of the larger transcription export (TREX) complex that couples messenger RNA processing and export. In humans, the transcription export complex is recruited to the 5'-end of messenger RNAs in a splicing- and cap-dependent manner. Studies of a related complex in mouse suggest that the metazoan transcription export complex is involved in cell differentiation and development. A pseudogene of this gene has been defined on chromosome 5. [provided by RefSeq, May 2013]

GeneCards Summary for THOC3 Gene

THOC3 (THO Complex 3) is a Protein Coding gene. Among its related pathways are Transport of Mature Transcript to Cytoplasm and Cleavage of Growing Transcript in the Termination Region. Gene Ontology (GO) annotations related to this gene include RNA binding.

UniProtKB/Swiss-Prot Summary for THOC3 Gene

  • Required for efficient export of polyadenylated RNA and spliced mRNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.

Additional gene information for THOC3 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for THOC3 Gene

Genomics for THOC3 Gene

GeneHancer (GH) Regulatory Elements for THOC3 Gene

Promoters and enhancers for THOC3 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH05J175967 Promoter/Enhancer 1.8 EPDnew Ensembl ENCODE CraniofacialAtlas 750.2 +66.9 66879 2.4 ZBTB40 SMARCE1 L3MBTL2 MNT POLR2A NKRF GTF2F1 SP7 ZNF687 SMARCA4 THOC3 FAF2 ENSG00000250909 ENSG00000251414 LOC105377745 ENSG00000250820
GH05J176034 Promoter/Enhancer 0.5 Ensembl dbSUPER 750.6 0.0 -21 1 ENSG00000250992 THOC3 LOC105377746
GH05J176033 Promoter/Enhancer 0.5 Ensembl dbSUPER 750.6 +1.0 979 0.6 THOC3 LOC105377746
GH05J176529 Promoter/Enhancer 2.7 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 6.6 -505.7 -505749 22.5 SIN3A SP1 ZBTB40 CTCF FOXA1 MLX SMARCE1 POLR2A TCF7L2 USF2 RNF44 CDHR2 FAF2 ZNF346 NSD1 ZNF346-IT1 ENSG00000251414 ENSG00000250909 LMAN2 MXD3
GH05J175919 Enhancer 0.8 Ensembl ENCODE 7 +112.8 112799 4.2 ZNF362 SP1 POLR2A RXRA ZNF148 EZH2 JUND FOS YY1 GATA3 THOC3 piR-58929-090 piR-33555-001 ENSG00000250820
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around THOC3 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the THOC3 gene promoter:
  • ARP-1
  • c-Ets-1
  • E47
  • Hand1
  • NRF-2
  • Pax-5
  • Pbx1a
  • RORalpha1
  • RP58
  • Sox9

Genomic Locations for THOC3 Gene

Genomic Locations for THOC3 Gene
chr5:175,917,873-176,034,680
(GRCh38/hg38)
Size:
116,808 bases
Orientation:
Minus strand
chr5:175,344,876-175,461,683
(GRCh37/hg19)
Size:
116,808 bases
Orientation:
Minus strand

Genomic View for THOC3 Gene

Genes around THOC3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
THOC3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for THOC3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for THOC3 Gene

Proteins for THOC3 Gene

  • Protein details for THOC3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96J01-THOC3_HUMAN
    Recommended name:
    THO complex subunit 3
    Protein Accession:
    Q96J01
    Secondary Accessions:
    • Q6NZ53

    Protein attributes for THOC3 Gene

    Size:
    351 amino acids
    Molecular mass:
    38772 Da
    Quaternary structure:
    • Component of the THO complex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7; together with at least ALYREF/THOC4, DDX39B, SARNP/CIP29 and CHTOP, THO forms the transcription/export (TREX) complex which seems to have a dynamic structure involving ATP-dependent remodeling.

    Alternative splice isoforms for THOC3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for THOC3 Gene

Post-translational modifications for THOC3 Gene

  • Ubiquitination at Lys77, Lys86, Lys171, and Lys343
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for THOC3 Gene

Domains & Families for THOC3 Gene

Gene Families for THOC3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins
  • Predicted secreted proteins

Suggested Antigen Peptide Sequences for THOC3 Gene

GenScript: Design optimal peptide antigens:
  • hTREX45 (THOC3_HUMAN)
genes like me logo Genes that share domains with THOC3: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for THOC3 Gene

Function for THOC3 Gene

Molecular function for THOC3 Gene

UniProtKB/Swiss-Prot Function:
Required for efficient export of polyadenylated RNA and spliced mRNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.

Phenotypes From GWAS Catalog for THOC3 Gene

Gene Ontology (GO) - Molecular Function for THOC3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003723 RNA binding IEA --
GO:0005515 protein binding IEA --
genes like me logo Genes that share ontologies with THOC3: view
genes like me logo Genes that share phenotypes with THOC3: view

Animal Model Products

CRISPR Products

miRNA for THOC3 Gene

miRTarBase miRNAs that target THOC3

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for THOC3

Clone Products

  • Addgene plasmids for THOC3

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for THOC3 Gene

Localization for THOC3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for THOC3 Gene

Nucleus. Nucleus speckle.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for THOC3 gene
Compartment Confidence
nucleus 5
plasma membrane 1
extracellular 1
cytosol 1
golgi apparatus 1
peroxisome 0

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for THOC3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000346 transcription export complex IDA 15833825
GO:0000445 THO complex part of transcription export complex IDA,IBA 23222130
GO:0000784 colocalizes_with nuclear chromosome, telomeric region IDA 24270157
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm TAS --
genes like me logo Genes that share ontologies with THOC3: view

Pathways & Interactions for THOC3 Gene

genes like me logo Genes that share pathways with THOC3: view

Gene Ontology (GO) - Biological Process for THOC3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006397 mRNA processing IEA --
GO:0006405 RNA export from nucleus TAS --
GO:0006406 mRNA export from nucleus TAS,IBA --
GO:0008380 RNA splicing IEA --
GO:0031124 mRNA 3'-end processing TAS --
genes like me logo Genes that share ontologies with THOC3: view

No data available for SIGNOR curated interactions for THOC3 Gene

Drugs & Compounds for THOC3 Gene

No Compound Related Data Available

Transcripts for THOC3 Gene

mRNA/cDNA for THOC3 Gene

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for THOC3

Clone Products

  • Addgene plasmids for THOC3

Alternative Splicing Database (ASD) splice patterns (SP) for THOC3 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5a · 5b · 5c ^ 6a · 6b · 6c ^ 7a · 7b
SP1: - - - -
SP2: - -
SP3: -
SP4: - -
SP5: - - -
SP6: -

Relevant External Links for THOC3 Gene

GeneLoc Exon Structure for
THOC3
ECgene alternative splicing isoforms for
THOC3

Expression for THOC3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for THOC3 Gene

mRNA differential expression in normal tissues according to GTEx for THOC3 Gene

This gene is overexpressed in Esophagus - Mucosa (x4.1).

Protein differential expression in normal tissues from HIPED for THOC3 Gene

This gene is overexpressed in Breast (20.9) and Urine (14.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for THOC3 Gene



Protein tissue co-expression partners for THOC3 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of THOC3 Gene:

THOC3

SOURCE GeneReport for Unigene cluster for THOC3 Gene:

Hs.484227

Evidence on tissue expression from TISSUES for THOC3 Gene

  • Nervous system(4.5)
genes like me logo Genes that share expression patterns with THOC3: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for THOC3 Gene

Orthologs for THOC3 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for THOC3 Gene

Organism Taxonomy Gene Similarity Type Details
platypus
(Ornithorhynchus anatinus)
Mammalia THOC3 33
  • 100 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia THOC3 33 32
  • 99.72 (n)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia THOC3 33
  • 94 (a)
OneToOne
dog
(Canis familiaris)
Mammalia THOC3 33 32
  • 92.78 (n)
OneToOne
cow
(Bos Taurus)
Mammalia THOC3 33 32
  • 92.4 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Thoc3 17 33 32
  • 89.08 (n)
rat
(Rattus norvegicus)
Mammalia Thoc3 32
  • 88.7 (n)
chicken
(Gallus gallus)
Aves THOC3 33 32
  • 83.75 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia THOC3 33
  • 94 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia thoc3 32
  • 80.38 (n)
Str.1963 32
African clawed frog
(Xenopus laevis)
Amphibia Xl.22312 32
zebrafish
(Danio rerio)
Actinopterygii thoc3 33 32
  • 79.79 (n)
OneToOne
Dr.13333 32
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.9463 32
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP003891 32
  • 64.4 (n)
fruit fly
(Drosophila melanogaster)
Insecta tex 33 32
  • 64.29 (n)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea thoc-3 33
  • 33 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes SIF2 33
  • 10 (a)
OneToMany
TEX1 35
thale cress
(Arabidopsis thaliana)
eudicotyledons TEX1 32
  • 55.93 (n)
soybean
(Glycine max)
eudicotyledons Gma.12953 32
Alicante grape
(Vitis vinifera)
eudicotyledons Vvi.5046 32
rice
(Oryza sativa)
Liliopsida Os09g0298400 32
  • 55.07 (n)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.2882 33
  • 66 (a)
OneToOne
Cin.1853 32
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.1853 32
Species where no ortholog for THOC3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for THOC3 Gene

ENSEMBL:
Gene Tree for THOC3 (if available)
TreeFam:
Gene Tree for THOC3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for THOC3: view image

Paralogs for THOC3 Gene

(3) SIMAP similar genes for THOC3 Gene using alignment to 9 proteins:

  • THOC3_HUMAN
  • D6REC9_HUMAN
  • D6RGZ2_HUMAN
  • H0Y9T1_HUMAN
  • H0YA11_HUMAN
  • H0YAG7_HUMAN
  • H0YAJ0_HUMAN
  • H7C0C5_HUMAN
  • Q6NZ53_HUMAN
genes like me logo Genes that share paralogs with THOC3: view

No data available for Paralogs for THOC3 Gene

Variants for THOC3 Gene

Sequence variations from dbSNP and Humsavar for THOC3 Gene

SNP ID Clin Chr 05 pos Variation AA Info Type
rs1000018214 -- 175,984,297(-) G/A genic_upstream_transcript_variant, intron_variant
rs1000387228 -- 176,012,561(-) G/A/C genic_upstream_transcript_variant, intron_variant
rs1000441356 -- 175,984,817(-) T/C genic_upstream_transcript_variant, intron_variant
rs1000885238 -- 176,013,139(-) A/G genic_upstream_transcript_variant, intron_variant
rs1001122606 -- 176,008,638(-) C/T genic_upstream_transcript_variant, intron_variant

Structural Variations from Database of Genomic Variants (DGV) for THOC3 Gene

Variant ID Type Subtype PubMed ID
dgv5832n100 CNV gain 25217958
dgv5833n100 CNV gain 25217958
esv2759395 CNV gain 17122850
esv34563 CNV gain 17911159
esv3575900 CNV gain 25503493
nsv1022071 CNV gain 25217958
nsv10776 CNV gain 18304495
nsv1146319 CNV duplication 26484159

Variation tolerance for THOC3 Gene

Residual Variation Intolerance Score: 67.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.37; 63.42% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for THOC3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
THOC3

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for THOC3 Gene

Disorders for THOC3 Gene

Additional Disease Information for THOC3

No disorders were found for THOC3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for THOC3 Gene

Publications for THOC3 Gene

  1. Recruitment of the human TREX complex to mRNA during splicing. (PMID: 15998806) Masuda S … Reed R (Genes & development 2005) 2 3 4 56
  2. TREX is a conserved complex coupling transcription with messenger RNA export. (PMID: 11979277) Strässer K … Hurt E (Nature 2002) 2 3 4 56
  3. Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA. (PMID: 23222130) Chi B … Cheng H (Nucleic acids research 2013) 3 4 56
  4. Recruitment of the complete hTREX complex is required for Kaposi's sarcoma-associated herpesvirus intronless mRNA nuclear export and virus replication. (PMID: 18974867) Boyne JR … Whitehouse A (PLoS pathogens 2008) 3 4 56
  5. Human mRNA export machinery recruited to the 5' end of mRNA. (PMID: 17190602) Cheng H … Reed R (Cell 2006) 3 4 56

Products for THOC3 Gene

Sources for THOC3 Gene