Aliases for THEM5 Gene

Aliases for THEM5 Gene

  • Thioesterase Superfamily Member 5 2 3 4 5
  • Acyl-Coenzyme A Thioesterase THEM5 3 4
  • Acyl-Coenzyme A Thioesterase 15 3 4
  • Acyl-CoA Thioesterase THEM5 3 4
  • ACOT15 3 4
  • EC 4

External Ids for THEM5 Gene

Previous GeneCards Identifiers for THEM5 Gene

  • GC01M148632
  • GC01M150084
  • GC01M151818
  • GC01M123196
  • GC01M151819

Summaries for THEM5 Gene

GeneCards Summary for THEM5 Gene

THEM5 (Thioesterase Superfamily Member 5) is a Protein Coding gene. Diseases associated with THEM5 include Median Rhomboid Glossitis and Petroclival Meningioma. Among its related pathways are Metabolism and Fatty Acyl-CoA Biosynthesis. Gene Ontology (GO) annotations related to this gene include palmitoyl-CoA hydrolase activity. An important paralog of this gene is THEM4.

UniProtKB/Swiss-Prot Summary for THEM5 Gene

  • Has acyl-CoA thioesterase activity towards long-chain (C16 and C18) fatty acyl-CoA substrates, with a preference for linoleoyl-CoA and other unsaturated long-chain fatty acid-CoA esters (PubMed:22586271). Plays an important role in mitochondrial fatty acid metabolism, and in remodeling of the mitochondrial lipid cardiolipin (PubMed:22586271). Required for normal mitochondrial function (PubMed:22586271).

Additional gene information for THEM5 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for THEM5 Gene

Genomics for THEM5 Gene

GeneHancer (GH) Regulatory Elements for THEM5 Gene

Promoters and enhancers for THEM5 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH01J151853 Promoter 0.6 EPDnew 750.6 0.0 -18 0.1 CEBPB RAD21 SMC3 THEM5 piR-53287 C2CD4D-AS1
GH01J151851 Enhancer 0.4 Ensembl 750.6 +1.8 1811 0.2 ZBTB33 ZNF692 ZFP69B THEM5 C2CD4D C2CD4D-AS1
GH01J151986 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 10.4 -140.8 -140771 16.2 PPP1R10 E2F8 SIN3A CTCF GATA3 SREBF1 SMARCE1 POLR2A TCF7L2 USF2 ENSG00000229021 S100A10 lnc-S100A11-2 THEM4 PI4KB PIP5K1A ZNF687 GABPB2 MRPL9 S100A11
GH01J151968 Enhancer 1.5 FANTOM5 Ensembl ENCODE dbSUPER 10.3 -116.8 -116769 7.8 PPP1R10 CTCF FOXA1 SMARCE1 TCF7L2 ZNF362 CC2D1A L3MBTL2 DACH1 RAD21 ENSG00000229021 THEM4 ZNF687 S100A10 PI4KB PIP5K1A S100A11 THEM5 CGN ENSG00000252840
GH01J151933 Enhancer 1.3 FANTOM5 Ensembl ENCODE dbSUPER 10.9 -82.0 -82005 3.6 CTCF CC2D1A RAD21 SP7 PRDM1 ZNF547 ZIC2 ARID2 REST TCF7L2 THEM4 ENSG00000229021 POGZ PIP5K1A PI4KB GABPB2 S100A10 MRPL9 LINGO4 TDRKH
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around THEM5 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the THEM5 gene promoter:
  • AP-1
  • c-Fos
  • c-Jun
  • C/EBPalpha
  • Evi-1
  • FosB
  • FOXO4
  • Fra-1

Genomic Locations for THEM5 Gene

Genomic Locations for THEM5 Gene
6,612 bases
Minus strand
6,597 bases
Minus strand

Genomic View for THEM5 Gene

Genes around THEM5 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
THEM5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for THEM5 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for THEM5 Gene

Proteins for THEM5 Gene

  • Protein details for THEM5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Acyl-coenzyme A thioesterase THEM5
    Protein Accession:
    Secondary Accessions:
    • Q5T1C3

    Protein attributes for THEM5 Gene

    247 amino acids
    Molecular mass:
    27677 Da
    Quaternary structure:
    • Homodimer.

    Three dimensional structures from OCA and Proteopedia for THEM5 Gene

neXtProt entry for THEM5 Gene

Post-translational modifications for THEM5 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for THEM5 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for THEM5 Gene

Domains & Families for THEM5 Gene

Gene Families for THEM5 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for THEM5 Gene


Suggested Antigen Peptide Sequences for THEM5 Gene

GenScript: Design optimal peptide antigens:
  • Thioesterase superfamily member 5 (B1APK3_HUMAN)
  • Thioesterase superfamily member 5 (THEM5_HUMAN)

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the THEM4/THEM5 thioesterase family.
  • Belongs to the THEM4/THEM5 thioesterase family.
genes like me logo Genes that share domains with THEM5: view

Function for THEM5 Gene

Molecular function for THEM5 Gene

UniProtKB/Swiss-Prot Function:
Has acyl-CoA thioesterase activity towards long-chain (C16 and C18) fatty acyl-CoA substrates, with a preference for linoleoyl-CoA and other unsaturated long-chain fatty acid-CoA esters (PubMed:22586271). Plays an important role in mitochondrial fatty acid metabolism, and in remodeling of the mitochondrial lipid cardiolipin (PubMed:22586271). Required for normal mitochondrial function (PubMed:22586271).
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + hexadecanoyl-CoA = CoA + H(+) + hexadecanoate; Xref=Rhea:RHEA:16645, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; EC=; Evidence=. ; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16646; Evidence=. ;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(9Z,12Z)-octadecadienoyl-CoA + H2O = (9Z,12Z)-octadecadienoate + CoA + H(+); Xref=Rhea:RHEA:40143, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30245, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383; Evidence=. ; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40144; Evidence=. ;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + tetradecanoyl-CoA = CoA + H(+) + tetradecanoate; Xref=Rhea:RHEA:40119, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385; Evidence=. ; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40120; Evidence=. ;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+); Xref=Rhea:RHEA:40139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387; Evidence=. ; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40140; Evidence=. ;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(9Z)-hexadecenoyl-CoA + H2O = (9Z)-hexadecenoate + CoA + H(+); Xref=Rhea:RHEA:40131, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32372, ChEBI:CHEBI:57287, ChEBI:CHEBI:61540; Evidence=. ; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40132; Evidence=. ;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA + H(+); Xref=Rhea:RHEA:40151, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:32395, ChEBI:CHEBI:57287, ChEBI:CHEBI:57368; Evidence=. ; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40152; Evidence=. ;.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=H2O + octadecanoyl-CoA = CoA + H(+) + octadecanoate; Xref=Rhea:RHEA:30139, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:25629, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394; Evidence=. ; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30140; Evidence=. ;.
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=2.4 uM for palmitoyl-CoA (C16:0) {ECO:0000269 PubMed:22586271}; KM=4.2 uM for stearoyl-CoA (C18:0) {ECO:0000269 PubMed:22586271}; KM=2.9 uM for oleoyl-CoA (C18:1) {ECO:0000269 PubMed:22586271}; KM=4.2 uM for linoleoyl-CoA (C18:2) {ECO:0000269 PubMed:22586271};

Enzyme Numbers (IUBMB) for THEM5 Gene

Phenotypes From GWAS Catalog for THEM5 Gene

Gene Ontology (GO) - Molecular Function for THEM5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 25416956
GO:0016290 palmitoyl-CoA hydrolase activity IEA,IDA 22586271
GO:0016787 hydrolase activity IEA --
GO:0102991 myristoyl-CoA hydrolase activity IEA --
genes like me logo Genes that share ontologies with THEM5: view
genes like me logo Genes that share phenotypes with THEM5: view

Animal Models for THEM5 Gene

MGI Knock Outs for THEM5:

Animal Model Products

CRISPR Products

Clone Products

No data available for Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for THEM5 Gene

Localization for THEM5 Gene

Subcellular locations from UniProtKB/Swiss-Prot for THEM5 Gene

Mitochondrion matrix.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for THEM5 gene
Compartment Confidence
mitochondrion 5
cytosol 2
extracellular 1
peroxisome 0
nucleus 0
golgi apparatus 0

Gene Ontology (GO) - Cellular Components for THEM5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005739 mitochondrion IEA --
GO:0005759 mitochondrial matrix TAS --
genes like me logo Genes that share ontologies with THEM5: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for THEM5 Gene

Pathways & Interactions for THEM5 Gene

genes like me logo Genes that share pathways with THEM5: view

Gene Ontology (GO) - Biological Process for THEM5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006629 lipid metabolic process IEA --
GO:0006631 fatty acid metabolic process IEA --
GO:0006637 acyl-CoA metabolic process TAS --
GO:0035336 long-chain fatty-acyl-CoA metabolic process IDA 22586271
GO:0035965 cardiolipin acyl-chain remodeling IEA,ISS --
genes like me logo Genes that share ontologies with THEM5: view

No data available for SIGNOR curated interactions for THEM5 Gene

Drugs & Compounds for THEM5 Gene

(3) Drugs for THEM5 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Palmitic Acid Approved Pharma Full agonist, Agonist 25
Water Approved Pharma 0
Coenzyme A Investigational Nutra 0

(1) Additional Compounds for THEM5 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
  • coenzyme A, S-Hexadecanoate
  • Palmitoyl coenzyme A
  • S-Palmitoylcoenzyme A
  • coenzyme A, S-Hexadecanoic acid
genes like me logo Genes that share compounds with THEM5: view

Transcripts for THEM5 Gene

mRNA/cDNA for THEM5 Gene

(1) REFSEQ mRNAs :
(3) Additional mRNA sequences :
(3) Selected AceView cDNA sequences:
(2) Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for THEM5 Gene

No ASD Table

Relevant External Links for THEM5 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for THEM5 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for THEM5 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for THEM5 Gene

This gene is overexpressed in Skin - Sun Exposed (Lower leg) (x24.7) and Skin - Not Sun Exposed (Suprapubic) (x19.1).

Protein differential expression in normal tissues from HIPED for THEM5 Gene

This gene is overexpressed in Skin (43.9) and Heart (25.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for THEM5 Gene

Protein tissue co-expression partners for THEM5 Gene

NURSA nuclear receptor signaling pathways regulating expression of THEM5 Gene:


SOURCE GeneReport for Unigene cluster for THEM5 Gene:


Evidence on tissue expression from TISSUES for THEM5 Gene

  • Heart(4)
  • Lung(4)
genes like me logo Genes that share expression patterns with THEM5: view

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for THEM5 Gene

Orthologs for THEM5 Gene

This gene was present in the common ancestor of chordates.

Orthologs for THEM5 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia THEM5 33 32
  • 96.21 (n)
(Canis familiaris)
Mammalia THEM5 33 32
  • 86.5 (n)
(Rattus norvegicus)
Mammalia Them5 32
  • 83.47 (n)
(Mus musculus)
Mammalia Them5 17 33 32
  • 83.4 (n)
(Bos Taurus)
Mammalia THEM5 33 32
  • 80.14 (n)
(Monodelphis domestica)
Mammalia THEM5 33
  • 61 (a)
(Ornithorhynchus anatinus)
Mammalia THEM5 33
  • 50 (a)
(Gallus gallus)
Aves -- 33
  • 32 (a)
(Anolis carolinensis)
Reptilia -- 33
  • 30 (a)
(Danio rerio)
Actinopterygii THEM4 33
  • 29 (a)
Species where no ortholog for THEM5 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for THEM5 Gene

Gene Tree for THEM5 (if available)
Gene Tree for THEM5 (if available)
Evolutionary constrained regions (ECRs) for THEM5: view image

Paralogs for THEM5 Gene

Paralogs for THEM5 Gene

(1) SIMAP similar genes for THEM5 Gene using alignment to 2 proteins:

  • H0Y6P4_HUMAN
genes like me logo Genes that share paralogs with THEM5: view

Variants for THEM5 Gene

Sequence variations from dbSNP and Humsavar for THEM5 Gene

SNP ID Clin Chr 01 pos Variation AA Info Type
rs1000610431 -- 151,850,936(-) A/C intron_variant
rs1000851200 -- 151,848,483(-) C/T intron_variant
rs1000964832 -- 151,855,077(-) C/T upstream_transcript_variant
rs1001637374 -- 151,852,516(-) G/T intron_variant
rs1001851328 -- 151,847,086(-) G/A downstream_transcript_variant

Structural Variations from Database of Genomic Variants (DGV) for THEM5 Gene

Variant ID Type Subtype PubMed ID
esv2717906 CNV deletion 23290073
esv2717918 CNV deletion 23290073
nsv516369 CNV gain 19592680

Variation tolerance for THEM5 Gene

Residual Variation Intolerance Score: 75.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.68; 46.07% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for THEM5 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for THEM5 Gene

Disorders for THEM5 Gene

MalaCards: The human disease database

(2) MalaCards diseases for THEM5 Gene - From: DISEASES

Disorder Aliases PubMed IDs
median rhomboid glossitis
  • persistent tuberculum impar
petroclival meningioma
- elite association - COSMIC cancer census association via MalaCards
Search THEM5 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for THEM5

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with THEM5: view

No data available for UniProtKB/Swiss-Prot and Genatlas for THEM5 Gene

Publications for THEM5 Gene

  1. Acyl coenzyme A thioesterase Them5/Acot15 is involved in cardiolipin remodeling and fatty liver development. (PMID: 22586271) Zhuravleva E … Hemmings BA (Molecular and cellular biology 2012) 3 4 56
  2. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PMID: 20877624) Hendrickson SL … O'Brien SJ (PloS one 2010) 3 43 56
  3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4 56
  4. A proteome-scale map of the human interactome network. (PMID: 25416956) Rolland T … Vidal M (Cell 2014) 3 56
  5. New genetic associations detected in a host response study to hepatitis B vaccine. (PMID: 20237496) Davila S … Seielstad M (Genes and immunity 2010) 3 56

Products for THEM5 Gene

Sources for THEM5 Gene