Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory sign... See more...

Aliases for TAF5 Gene

Aliases for TAF5 Gene

  • TATA-Box Binding Protein Associated Factor 5 2 3 5
  • TAFII100 2 3 4
  • TAF5 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 100kDa 2 3
  • TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase II, D, 100kD 2 3
  • Transcription Initiation Factor TFIID 100 KDa Subunit 3 4
  • Transcription Initiation Factor TFIID Subunit 5 3 4
  • TAF(II)100 3 4
  • TAFII-100 3 4
  • TAF2D 3 4
  • Transcription Initiation Factor TFIID 100 KD Subunit 3
  • TATA Box Binding Protein (TBP)-Associated Factor 2D 3
  • TAF5 5

External Ids for TAF5 Gene

Previous HGNC Symbols for TAF5 Gene

  • TAF2D

Previous GeneCards Identifiers for TAF5 Gene

  • GC10P104020
  • GC10P104361
  • GC10P105258
  • GC10P104792
  • GC10P105117
  • GC10P098758

Summaries for TAF5 Gene

Entrez Gene Summary for TAF5 Gene

  • Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

GeneCards Summary for TAF5 Gene

TAF5 (TATA-Box Binding Protein Associated Factor 5) is a Protein Coding gene. Among its related pathways are RNA Polymerase II Transcription Initiation And Promoter Clearance and Regulation of TP53 Activity. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and transcription regulatory region DNA binding. An important paralog of this gene is TAF5L.

UniProtKB/Swiss-Prot Summary for TAF5 Gene

  • TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed.

Gene Wiki entry for TAF5 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for TAF5 Gene

Genomics for TAF5 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for TAF5 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH10J103367 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 611 +0.4 425 2.8 SP1 CREB1 GATAD2A PRDM10 ZNF629 TFE3 ZNF692 POLR2A NFRKB BACH1 TAF5 SFXN2 SLK CALHM2 PDCD11 RF00017-792 ATP5MD
GH10J102853 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 28.5 -512.8 -512799 3.2 HNRNPL CREB1 GATAD2A ATF7 TEAD4 PRDM10 ZNF629 TFE3 RFX1 SIX5 BORCS7 BORCS7-ASMT ENSG00000282772 AS3MT PDCD11 TAF5 GBF1 LDB1 NOLC1 RPEL1
GH10J104316 Promoter/Enhancer 2.7 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 19.9 +960.8 960795 24.8 ZNF221 CEBPG SP1 HNRNPL CREB1 ATF7 PRDM10 ZNF629 REST RFX1 CFAP58 ITPRIP PDCD11 ENSG00000260461 GSTO2 TAF5 SFR1 CFAP58-DT PCGF6 GSTO1
GH10J103277 Promoter/Enhancer 1.5 EPDnew Ensembl ENCODE 27.9 -89.8 -89776 2.8 REST TFE3 NFKBIZ ZNF692 ZNF143 ZSCAN4 POLR2A EZH2 CTCF ZNF600 INA lnc-NT5C2-1 PDCD11 TAF5 ENSG00000260461 RNU11-3P SUFU PCGF6 PFN1P11 PTGES3P4
GH10J102641 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 13.7 -720.3 -720263 12.7 SP1 HNRNPL GATAD2A CTCF ZBTB33 PRDM10 ZNF629 REST TFE3 LEF1 TRIM8 SFXN2 MFSD13A NOLC1 PDCD11 AS3MT PPRC1 TAF5 LDB1 ARL3
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around TAF5 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for TAF5

Top Transcription factor binding sites by QIAGEN in the TAF5 gene promoter:
  • AML1a
  • c-Myb
  • HOXA9
  • HOXA9B
  • Meis-1
  • Meis-1b
  • Pax-5
  • SEF-1 (1)
  • YY1

Genomic Locations for TAF5 Gene

Genomic Locations for TAF5 Gene
chr10:103,367,976-103,389,065
(GRCh38/hg38)
Size:
21,090 bases
Orientation:
Plus strand
chr10:105,127,724-105,148,822
(GRCh37/hg19)
Size:
21,099 bases
Orientation:
Plus strand

Genomic View for TAF5 Gene

Genes around TAF5 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
TAF5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for TAF5 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for TAF5 Gene

Proteins for TAF5 Gene

  • Protein details for TAF5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q15542-TAF5_HUMAN
    Recommended name:
    Transcription initiation factor TFIID subunit 5
    Protein Accession:
    Q15542
    Secondary Accessions:
    • A8K5B4
    • B2RMR0
    • B7ZKJ6
    • Q53EM4
    • Q5SYD5
    • Q86UZ7
    • Q9Y4K5

    Protein attributes for TAF5 Gene

    Size:
    800 amino acids
    Molecular mass:
    86830 Da
    Quaternary structure:
    • Homodimer. TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H/SPT3, TAF2/TAFII150, TAF4/TAFII135, TAF5/TAFII100, GCN5L2/GCN5, TAF10 and TRRAP.
    • (Microbial infection) Interacts with SV40 Large T antigen.
    SequenceCaution:
    • Sequence=AAC50902.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAD97335.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for TAF5 Gene

    Alternative splice isoforms for TAF5 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for TAF5 Gene

Post-translational modifications for TAF5 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for TAF5 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Abcam antibodies for TAF5

No data available for DME Specific Peptides for TAF5 Gene

Domains & Families for TAF5 Gene

Gene Families for TAF5 Gene

Protein Domains for TAF5 Gene

InterPro:
Blocks:
  • G-protein beta WD-40 repeat signature
  • Lissencephaly type-1-like homology motif
  • WD40 associated region in TFIID subunit
ProtoNet:

Suggested Antigen Peptide Sequences for TAF5 Gene

GenScript: Design optimal peptide antigens:
  • Transcription initiation factor TFIID 100 kDa subunit (TAF5_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q15542

UniProtKB/Swiss-Prot:

TAF5_HUMAN :
  • Distinct domains of TAF5/TAFII100 are required for functional interaction with transcription factor TFIIFB (RAP30) and incorporation into the TFIID complex.
  • Belongs to the WD repeat TAF5 family.
Domain:
  • Distinct domains of TAF5/TAFII100 are required for functional interaction with transcription factor TFIIFB (RAP30) and incorporation into the TFIID complex.
Family:
  • Belongs to the WD repeat TAF5 family.
genes like me logo Genes that share domains with TAF5: view

Function for TAF5 Gene

Molecular function for TAF5 Gene

UniProtKB/Swiss-Prot Function:
TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed.

Phenotypes From GWAS Catalog for TAF5 Gene

Gene Ontology (GO) - Molecular Function for TAF5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000976 transcription regulatory region sequence-specific DNA binding IDA 17996705
GO:0003713 transcription coactivator activity IBA 21873635
GO:0005515 protein binding IPI 9045704
GO:0016251 RNA polymerase II general transcription initiation factor activity IMP 9045704
GO:0042802 identical protein binding IPI 17227857
genes like me logo Genes that share ontologies with TAF5: view
genes like me logo Genes that share phenotypes with TAF5: view

Animal Model Products

CRISPR Products

miRNA for TAF5 Gene

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for TAF5

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for TAF5 Gene

Localization for TAF5 Gene

Subcellular locations from UniProtKB/Swiss-Prot for TAF5 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for TAF5 gene
Compartment Confidence
nucleus 5
cytoskeleton 4
cytosol 2
mitochondrion 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for TAF5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000790 nuclear chromatin IDA 24289924
GO:0005634 nucleus IEA,IDA 15637059
GO:0005654 nucleoplasm TAS --
GO:0005669 transcription factor TFIID complex IDA 9045704
GO:0005730 NOT nucleolus IDA 15637059
genes like me logo Genes that share ontologies with TAF5: view

Pathways & Interactions for TAF5 Gene

genes like me logo Genes that share pathways with TAF5: view

Pathways by source for TAF5 Gene

1 GeneGo (Thomson Reuters) pathway for TAF5 Gene
  • Transcription Ligand-Dependent Transcription of Retinoid-Target genes
7 Qiagen pathways for TAF5 Gene
  • Assembly of RNA Polymerase-II Initiation Complex
  • Crosstalk Between CARM1 and ESRs
  • Estrogen Pathway
  • Glucocorticoid Receptor Signaling
  • Nuclear Receptor Activation by Vitamin-A

Gene Ontology (GO) - Biological Process for TAF5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006352 DNA-templated transcription, initiation IDA 9603525
GO:0006366 transcription by RNA polymerase II TAS --
GO:0006367 transcription initiation from RNA polymerase II promoter TAS --
GO:0016032 viral process IEA --
GO:0042795 snRNA transcription by RNA polymerase II TAS --
genes like me logo Genes that share ontologies with TAF5: view

No data available for SIGNOR curated interactions for TAF5 Gene

Drugs & Compounds for TAF5 Gene

No Compound Related Data Available

Transcripts for TAF5 Gene

mRNA/cDNA for TAF5 Gene

2 REFSEQ mRNAs :
11 NCBI additional mRNA sequence :
1 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for TAF5

Alternative Splicing Database (ASD) splice patterns (SP) for TAF5 Gene

No ASD Table

Relevant External Links for TAF5 Gene

GeneLoc Exon Structure for
TAF5

Expression for TAF5 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for TAF5 Gene

Protein differential expression in normal tissues from HIPED for TAF5 Gene

This gene is overexpressed in Liver, secretome (18.3) and Lymph node (11.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for TAF5 Gene



Protein tissue co-expression partners for TAF5 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for TAF5

SOURCE GeneReport for Unigene cluster for TAF5 Gene:

Hs.96103

Evidence on tissue expression from TISSUES for TAF5 Gene

  • Nervous system(4.4)
  • Skin(2.1)
  • Kidney(2.1)
genes like me logo Genes that share expression patterns with TAF5: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for TAF5 Gene

Orthologs for TAF5 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for TAF5 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia TAF5 30 31
  • 99.71 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 95 (a)
OneToMany
-- 31
  • 94 (a)
OneToMany
Dog
(Canis familiaris)
Mammalia TAF5 30 31
  • 94.08 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia TAF5 30 31
  • 93.92 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Taf5 30
  • 91.85 (n)
Mouse
(Mus musculus)
Mammalia Taf5 30 17 31
  • 91.33 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia TAF5 31
  • 88 (a)
OneToOne
Chicken
(Gallus gallus)
Aves TAF5 30 31
  • 78.69 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia TAF5 31
  • 80 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia taf5 30
  • 75.41 (n)
Zebrafish
(Danio rerio)
Actinopterygii taf5 30 31
  • 72.12 (n)
OneToOne
wufc38g05 30
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.13815 30
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP011678 30
  • 54.53 (n)
Fruit Fly
(Drosophila melanogaster)
Insecta Taf5 30 31 32
  • 52.37 (n)
OneToOne
CG4448 32
  • 40 (a)
can 32
  • 34 (a)
Worm
(Caenorhabditis elegans)
Secernentea taf-5 30
  • 48.49 (n)
K. Lactis Yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0E23453g 30
  • 50.67 (n)
A. gosspyii yeast
(Eremothecium gossypii)
Saccharomycetes AGOS_AEL246C 30
  • 47.67 (n)
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes TAF5 30 31 33
  • 42.13 (n)
OneToMany
Thale Cress
(Arabidopsis thaliana)
eudicotyledons TAF5 30
  • 48.85 (n)
Rice
(Oryza sativa)
Liliopsida Os06g0649500 30
  • 49.41 (n)
Wheat
(Triticum aestivum)
Liliopsida Ta.7323 30
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.2112 31
  • 52 (a)
OneToOne
Fission Yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes taf5 30
  • 46.73 (n)
Species where no ortholog for TAF5 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for TAF5 Gene

ENSEMBL:
Gene Tree for TAF5 (if available)
TreeFam:
Gene Tree for TAF5 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for TAF5: view image

Paralogs for TAF5 Gene

Paralogs for TAF5 Gene

(1) SIMAP similar genes for TAF5 Gene using alignment to 1 proteins:

  • TAF5_HUMAN
genes like me logo Genes that share paralogs with TAF5: view

Variants for TAF5 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for TAF5 Gene

SNP ID Clinical significance and condition Chr 10 pos Variation AA Info Type
708795 Benign: not provided 103,381,735(+) G/C SYNONYMOUS_VARIANT
818167 Not Provided: not provided 103,379,720(+) G/A MISSENSE_VARIANT
rs10883859 - p.Ser130Ala

Additional dbSNP identifiers (rs#s) for TAF5 Gene

Structural Variations from Database of Genomic Variants (DGV) for TAF5 Gene

Variant ID Type Subtype PubMed ID
dgv965n100 CNV gain 25217958
dgv966n100 CNV gain 25217958

Variation tolerance for TAF5 Gene

Residual Variation Intolerance Score: 19% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 8.97; 87.32% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for TAF5 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
TAF5

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for TAF5 Gene

Disorders for TAF5 Gene

No disorders were found for TAF5 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for TAF5 Gene

Publications for TAF5 Gene

  1. Molecular cloning and analysis of two subunits of the human TFIID complex: hTAFII130 and hTAFII100. (PMID: 8942982) Tanese N … Admon A (Proceedings of the National Academy of Sciences of the United States of America 1996) 2 3 4
  2. Structural analysis and dimerization potential of the human TAF5 subunit of TFIID. (PMID: 17227857) Bhattacharya S … Jacobson RH (Proceedings of the National Academy of Sciences of the United States of America 2007) 3 4
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4
  4. Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis. (PMID: 12601814) Cavusoglu N … Van Dorsselaer A (Proteomics 2003) 3 4
  5. Identification of TATA-binding protein-free TAFII-containing complex subunits suggests a role in nucleosome acetylation and signal transduction. (PMID: 10373431) Brand M … Tora L (The Journal of biological chemistry 1999) 3 4

Products for TAF5 Gene

Sources for TAF5 Gene