Aliases for SPOUT1 Gene

Aliases for SPOUT1 Gene

  • SPOUT Domain Containing Methyltransferase 1 2 3 5
  • Centromere Protein 32 2 3 4
  • CENP-32 2 3 4
  • Putative Methyltransferase C9orf114 3 4
  • Kinetochore-Associated Protein 3 4
  • C9orf114 3 4
  • HSPC109 2 3
  • SPOUT Domain-Containing Methyltransferase 1 4
  • Chromosome 9 Open Reading Frame 114 2
  • EC 2.1.1.- 4
  • CENP32 3
  • SPOUT1 5

External Ids for SPOUT1 Gene

Previous HGNC Symbols for SPOUT1 Gene

  • C9orf114

Summaries for SPOUT1 Gene

GeneCards Summary for SPOUT1 Gene

SPOUT1 (SPOUT Domain Containing Methyltransferase 1) is a Protein Coding gene. Diseases associated with SPOUT1 include Basal Cell Carcinoma, Infundibulocystic and Skin Sarcoma.

UniProtKB/Swiss-Prot Summary for SPOUT1 Gene

  • Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase (PubMed:20813266, PubMed:25657325). Also involved in chromosome alignment (PubMed:20813266). May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis (PubMed:25657325). Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level (PubMed:28431233).

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for SPOUT1 Gene

Genomics for SPOUT1 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for SPOUT1 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around SPOUT1 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for SPOUT1

Genomic Locations for SPOUT1 Gene

Latest Assembly
chr9:128,819,651-128,833,309
(GRCh38/hg38)
Size:
13,659 bases
Orientation:
Minus strand

Previous Assembly
chr9:131,581,930-131,592,073
(GRCh37/hg19 by Entrez Gene)
Size:
10,144 bases
Orientation:
Minus strand

chr9:131,581,930-131,592,100
(GRCh37/hg19 by Ensembl)
Size:
10,171 bases
Orientation:
Minus strand

Genomic View for SPOUT1 Gene

Genes around SPOUT1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
SPOUT1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for SPOUT1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPOUT1 Gene

Proteins for SPOUT1 Gene

  • Protein details for SPOUT1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q5T280-CI114_HUMAN
    Recommended name:
    Putative methyltransferase C9orf114
    Protein Accession:
    Q5T280
    Secondary Accessions:
    • Q0D2P6
    • Q6P469
    • Q6PGP9
    • Q6PIJ1
    • Q6PJV9

    Protein attributes for SPOUT1 Gene

    Size:
    376 amino acids
    Molecular mass:
    42009 Da
    Quaternary structure:
    • Interacts with INCA1.
    Miscellaneous:
    • Depletion with RNAi causes a significant accumulation of cells in later prometaphase with misaligned chromosomes.

    Three dimensional structures from OCA and Proteopedia for SPOUT1 Gene

neXtProt entry for SPOUT1 Gene

Post-translational modifications for SPOUT1 Gene

  • Ubiquitination at Lys66
  • Modification sites at PhosphoSitePlus

Other Protein References for SPOUT1 Gene

No data available for DME Specific Peptides for SPOUT1 Gene

Domains & Families for SPOUT1 Gene

Gene Families for SPOUT1 Gene

Protein Domains for SPOUT1 Gene

InterPro:
Blocks:
  • Protein of unknown function DUF171

Suggested Antigen Peptide Sequences for SPOUT1 Gene

GenScript: Design optimal peptide antigens:
  • HSPC109 (Q9P041_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q5T280

UniProtKB/Swiss-Prot:

CI114_HUMAN :
  • Belongs to the class IV-like SAM-binding methyltransferase superfamily.
Family:
  • Belongs to the class IV-like SAM-binding methyltransferase superfamily.
genes like me logo Genes that share domains with SPOUT1: view

Function for SPOUT1 Gene

Molecular function for SPOUT1 Gene

UniProtKB/Swiss-Prot Function:
Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase (PubMed:20813266, PubMed:25657325). Also involved in chromosome alignment (PubMed:20813266). May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis (PubMed:25657325). Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level (PubMed:28431233).

Enzyme Numbers (IUBMB) for SPOUT1 Gene

Phenotypes From GWAS Catalog for SPOUT1 Gene

Gene Ontology (GO) - Molecular Function for SPOUT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003723 RNA binding IEA,HDA 22658674
GO:0005515 protein binding IPI 21750715
GO:0008168 methyltransferase activity IEA --
GO:0016740 transferase activity IEA --
GO:0035198 miRNA binding IBA,IDA 28431233
genes like me logo Genes that share ontologies with SPOUT1: view
genes like me logo Genes that share phenotypes with SPOUT1: view

Animal Models for SPOUT1 Gene

MGI Knock Outs for SPOUT1:

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for SPOUT1

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for SPOUT1 Gene

Localization for SPOUT1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPOUT1 Gene

Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associated with the outer kinetochore. {ECO:0000269 PubMed:20813266}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for SPOUT1 gene
Compartment Confidence
cytoskeleton 5
nucleus 4
cytosol 2

Gene Ontology (GO) - Cellular Components for SPOUT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000775 chromosome, centromeric region IEA --
GO:0000776 kinetochore IBA,IDA 25657325
GO:0000777 condensed chromosome kinetochore IEA --
GO:0005694 chromosome IEA --
GO:0005737 cytoplasm IEA --
genes like me logo Genes that share ontologies with SPOUT1: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for SPOUT1 Gene

Pathways & Interactions for SPOUT1 Gene

PathCards logo

SuperPathways for SPOUT1 Gene

No Data Available

Gene Ontology (GO) - Biological Process for SPOUT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007049 cell cycle IEA --
GO:0010608 posttranscriptional regulation of gene expression IMP 28431233
GO:0032259 methylation IEA --
GO:0035196 production of miRNAs involved in gene silencing by miRNA IMP 28431233
GO:0051301 cell division IEA --
genes like me logo Genes that share ontologies with SPOUT1: view

No data available for Pathways by source and SIGNOR curated interactions for SPOUT1 Gene

Drugs & Compounds for SPOUT1 Gene

No Compound Related Data Available

Transcripts for SPOUT1 Gene

mRNA/cDNA for SPOUT1 Gene

1 REFSEQ mRNAs :
11 NCBI additional mRNA sequence :
5 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for SPOUT1

Alternative Splicing Database (ASD) splice patterns (SP) for SPOUT1 Gene

No ASD Table

Relevant External Links for SPOUT1 Gene

GeneLoc Exon Structure for
SPOUT1

Expression for SPOUT1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

  • Testis (Reproductive System)

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for SPOUT1

Evidence on tissue expression from TISSUES for SPOUT1 Gene

  • Nervous system(4.4)
  • Blood(4.1)
  • Eye(4)
  • Skin(2)
genes like me logo Genes that share expression patterns with SPOUT1: view

Primer products for research

No data available for mRNA expression in normal human tissues , mRNA differential expression in normal tissues , Protein differential expression in normal tissues , Protein expression , Protein tissue co-expression partners , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for SPOUT1 Gene

Orthologs for SPOUT1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for SPOUT1 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia C9H9orf114 29
  • 99.38 (n)
C9orf114 30
  • 59 (a)
OneToOne
Cow
(Bos Taurus)
Mammalia C9orf114 30
  • 93 (a)
OneToOne
C11H9orf114 29
  • 88.59 (n)
Dog
(Canis familiaris)
Mammalia C9orf114 30
  • 93 (a)
OneToOne
C9H9orf114 29
  • 88.92 (n)
Mouse
(Mus musculus)
Mammalia D2Wsu81e 29 30
  • 85.65 (n)
OneToOne
Spout1 16
Platypus
(Ornithorhynchus anatinus)
Mammalia C9orf114 30
  • 65 (a)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia C9orf114 30
  • 48 (a)
OneToOne
Chicken
(Gallus gallus)
Aves C17H9orf114 29
  • 72.33 (n)
C17H9ORF114 30
  • 71 (a)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia C9orf114 30
  • 73 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia c9orf114 29
  • 67.78 (n)
Str.1277 29
African clawed frog
(Xenopus laevis)
Amphibia Xl.26356 29
Zebrafish
(Danio rerio)
Actinopterygii zgc:101748 29
  • 69.25 (n)
C21H9orf114 30
  • 65 (a)
OneToOne
sbcb90 29
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP007570 29
  • 58.99 (n)
Fruit Fly
(Drosophila melanogaster)
Insecta CG12128 29 30
  • 56.12 (n)
OneToOne
Worm
(Caenorhabditis elegans)
Secernentea B0361.6 29 30
  • 48.74 (n)
OneToOne
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes -- 30
  • 22 (a)
OneToMany
-- 30
  • 21 (a)
OneToMany
-- 32
Thale Cress
(Arabidopsis thaliana)
eudicotyledons AT5G19300 29
  • 51.01 (n)
Rice
(Oryza sativa)
Liliopsida Os04g0244500 29
  • 54.07 (n)
Os.9692 29
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 30
  • 55 (a)
OneToOne
Bread mold
(Neurospora crassa)
Ascomycetes NCU00597 29
  • 50.63 (n)
Sea Vase
(Ciona intestinalis)
Ascidiacea Cin.5582 29
Species where no ortholog for SPOUT1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rat (Rattus norvegicus)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for SPOUT1 Gene

ENSEMBL:
Gene Tree for SPOUT1 (if available)
TreeFam:
Gene Tree for SPOUT1 (if available)
Alliance of Genome Resources:
Additional Orthologs for SPOUT1

Paralogs for SPOUT1 Gene

No data available for Paralogs for SPOUT1 Gene

Variants for SPOUT1 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for SPOUT1 Gene

SNP ID Clinical significance and condition Chr 09 pos Variation AA Info Type
rs1465223718 Uncertain Significance: not provided 128,827,144(-) T/C
NM_016390.4(SPOUT1):c.256A>G (p.Asn86Asp)
MISSENSE
rs151128931 Benign: not provided 128,828,754(-) C/T
NM_016390.4(SPOUT1):c.189G>A (p.Ala63=)
SYNONYMOUS
rs55985529 Benign: not provided 128,822,370(-) C/T
NM_004435.2(ENDOG):c.654C>T (p.Ile218=)
SYNONYMOUS_VARIANT,THREE_PRIME_UTR
rs61737987 Benign: not provided 128,822,453(-) C/T
NM_004435.2(ENDOG):c.737C>T (p.Thr246Ile)
MISSENSE_VARIANT,THREE_PRIME_UTR
rs61737988 Benign: not provided 128,822,450(-) G/T
NM_004435.2(ENDOG):c.734G>T (p.Arg245Leu)
MISSENSE_VARIANT,THREE_PRIME_UTR

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for SPOUT1 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for SPOUT1 Gene

Variant ID Type Subtype PubMed ID
nsv521120 CNV loss 19592680
nsv615411 CNV loss 21841781
nsv615412 CNV loss 21841781
nsv6724 CNV insertion 18451855

Variation tolerance for SPOUT1 Gene

Residual Variation Intolerance Score: 40.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 11.63; 93.13% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for SPOUT1 Gene

Human Gene Mutation Database (HGMD)
SPOUT1
SNPedia medical, phenotypic, and genealogical associations of SNPs for
SPOUT1
Leiden Open Variation Database (LOVD)
SPOUT1

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SPOUT1 Gene

Disorders for SPOUT1 Gene

MalaCards: The human disease database

(2) MalaCards diseases for SPOUT1 Gene - From: COP

Disorder Aliases PubMed IDs
basal cell carcinoma, infundibulocystic
  • basal cell carcinoma with follicular differentiation
skin sarcoma
  • cutaneous sarcoma
- elite association - COSMIC cancer census association via MalaCards

Additional Disease Information for SPOUT1

genes like me logo Genes that share disorders with SPOUT1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for SPOUT1 Gene

Publications for SPOUT1 Gene

  1. CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly. (PMID: 25657325) Ohta S … Earnshaw WC (Molecular biology of the cell 2015) 2 3 4
  2. The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics. (PMID: 20813266) Ohta S … Rappsilber J (Cell 2010) 2 3 4
  3. A Compendium of RNA-Binding Proteins that Regulate MicroRNA Biogenesis. (PMID: 28431233) Treiber T … Meister G (Molecular cell 2017) 3 4
  4. The inhibitor of growth protein 5 (ING5) depends on INCA1 as a co-factor for its antiproliferative effects. (PMID: 21750715) Zhang F … Müller-Tidow C (PloS one 2011) 3 4
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4

Products for SPOUT1 Gene

Sources for SPOUT1 Gene