The protein encoded by this gene is a sperm surface protein that may be involved in adhesion to the egg prior to fertilization. While the encoded protein has significant similarity to lysozyme at the amino acid level, it has no detectable bacteriocidal activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015] See more...

Aliases for SPACA3 Gene

Aliases for SPACA3 Gene

  • Sperm Acrosome Associated 3 2 3 5
  • Cancer/Testis Antigen 54 2 3 4
  • Lysozyme-Like Acrosomal Sperm-Specific Secretory Protein ALLP-17 3 4
  • Sperm Protein Reactive With Antisperm Antibodies 3 4
  • Sperm Acrosome Membrane-Associated Protein 3 3 4
  • Sperm Lyzozyme-Like Acrosomal Protein 1 2 3
  • Sperm Protein Reactive With ASA 3 4
  • Sperm Lysozyme-Like Protein 1 3 4
  • Lysozyme-Like Protein 3 3 4
  • LYZL3 3 4
  • SLLP1 3 4
  • CT54 3 4
  • LYC3 3 4
  • Lysozyme-Like Sperm-Specific Secretory Protein ALLP17 3
  • Lysozyme C 3
  • ALLP17 3
  • SPRASA 4
  • LYZC 3

External Ids for SPACA3 Gene

Previous GeneCards Identifiers for SPACA3 Gene

  • GC00U991055
  • GC17P031167
  • GC17P031464
  • GC17P028342
  • GC17P031318
  • GC17P027504

Summaries for SPACA3 Gene

Entrez Gene Summary for SPACA3 Gene

  • The protein encoded by this gene is a sperm surface protein that may be involved in adhesion to the egg prior to fertilization. While the encoded protein has significant similarity to lysozyme at the amino acid level, it has no detectable bacteriocidal activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

GeneCards Summary for SPACA3 Gene

SPACA3 (Sperm Acrosome Associated 3) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include lysozyme activity. An important paralog of this gene is LYZL1.

UniProtKB/Swiss-Prot Summary for SPACA3 Gene

  • Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro.

Gene Wiki entry for SPACA3 Gene

Additional gene information for SPACA3 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for SPACA3 Gene

Genomics for SPACA3 Gene

GeneHancer (GH) Regulatory Elements for SPACA3 Gene

Promoters and enhancers for SPACA3 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH17J032969 Enhancer 1 Ensembl ENCODE 762.3 +0.6 609 2.4 SMARCE1 RUNX1 ETV6 DACH1 TEAD4 ZNF148 CBFA2T2 BACH1 MLLT1 SMARCA4 SPACA3 piR-61101-276 TMEM98 piR-36588-188
GH17J032991 Promoter 0.3 EPDnew 750.3 +21.5 21520 0.1 SPACA3 piR-36588-188 piR-41306-112
GH17J032927 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE CraniofacialAtlas 6.6 -41.8 -41784 3.2 ZBTB40 MLX RBPJ SMARCE1 NR2F6 MIXL1 NFYC ZNF121 ZNF148 GTF2F1 TMEM98 ZNF207 SUZ12 ENSG00000265139 SH3GL1P1 LRRC37B SPACA3 RF00017-2353
GH17J032961 Enhancer 0.8 Ensembl ENCODE 10.8 -8.5 -8473 3 SMARCE1 ZKSCAN8 CBFA2T2 IKZF1 HDAC1 SOX6 TCF12 CTBP1 GATAD2A ZSCAN29 TMEM98 SPACA3 piR-37972-275 piR-61101-275
GH17J032951 Enhancer 0.7 Ensembl ENCODE 10.7 -16.5 -16532 4 ZNF24 SP1 BCOR TFAP4 HNF4A NFIA ZMYM3 TMEM98 SPACA3 piR-61101-275 piR-37972-275
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around SPACA3 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the SPACA3 gene promoter:
  • AP-4
  • c-Myb
  • ER-alpha
  • Hlf
  • HSF2
  • LCR-F1
  • p53
  • POU3F2
  • POU6F1 (c2)
  • RFX1

Genomic Locations for SPACA3 Gene

Genomic Locations for SPACA3 Gene
chr17:32,970,376-32,997,877
(GRCh38/hg38)
Size:
27,502 bases
Orientation:
Plus strand
chr17:31,297,394-31,324,895
(GRCh37/hg19)
Size:
27,502 bases
Orientation:
Plus strand

Genomic View for SPACA3 Gene

Genes around SPACA3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
SPACA3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for SPACA3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SPACA3 Gene

Proteins for SPACA3 Gene

  • Protein details for SPACA3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8IXA5-SACA3_HUMAN
    Recommended name:
    Sperm acrosome membrane-associated protein 3
    Protein Accession:
    Q8IXA5
    Secondary Accessions:
    • Q7Z4Y5

    Protein attributes for SPACA3 Gene

    Size:
    215 amino acids
    Molecular mass:
    23431 Da
    Quaternary structure:
    • Interacts with ASTL.

    Alternative splice isoforms for SPACA3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SPACA3 Gene

Post-translational modifications for SPACA3 Gene

  • The processed form derives from the membrane form by proteolytic processing.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for SPACA3 Gene

No data available for DME Specific Peptides for SPACA3 Gene

Domains & Families for SPACA3 Gene

Gene Families for SPACA3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins
  • Predicted membrane proteins
  • Predicted secreted proteins

Protein Domains for SPACA3 Gene

Suggested Antigen Peptide Sequences for SPACA3 Gene

GenScript: Design optimal peptide antigens:
  • Sperm protein reactive with antisperm antibodies (SACA3_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q8IXA5

UniProtKB/Swiss-Prot:

SACA3_HUMAN :
  • Belongs to the glycosyl hydrolase 22 family.
Family:
  • Belongs to the glycosyl hydrolase 22 family.
genes like me logo Genes that share domains with SPACA3: view

Function for SPACA3 Gene

Molecular function for SPACA3 Gene

UniProtKB/Swiss-Prot Function:
Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro.

Phenotypes From GWAS Catalog for SPACA3 Gene

Gene Ontology (GO) - Molecular Function for SPACA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003796 NOT lysozyme activity IDA,IEA 12606493
GO:0005515 protein binding IPI 19945174
genes like me logo Genes that share ontologies with SPACA3: view
genes like me logo Genes that share phenotypes with SPACA3: view

Animal Models for SPACA3 Gene

MGI Knock Outs for SPACA3:

Animal Model Products

CRISPR Products

miRNA for SPACA3 Gene

miRTarBase miRNAs that target SPACA3

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for SPACA3

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for SPACA3 Gene

Localization for SPACA3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SPACA3 Gene

Isoform 1: Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Single-pass type II membrane protein. Note=Anterior acrosome in non-capacitated spermatozoa and retained in the equatorial segment and in the luminal face of both the inner and outer acrosomal membranes following capacitation and the acrosome reaction. {ECO:0000250}.
Isoform 2: Secreted.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for SPACA3 gene
Compartment Confidence
extracellular 4
mitochondrion 2

Gene Ontology (GO) - Cellular Components for SPACA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002080 acrosomal membrane IDA,IEA 12606493
GO:0005576 extracellular region IEA --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
GO:0030141 secretory granule IDA 3470418
genes like me logo Genes that share ontologies with SPACA3: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for SPACA3 Gene

Pathways & Interactions for SPACA3 Gene

PathCards logo

SuperPathways for SPACA3 Gene

No Data Available

Gene Ontology (GO) - Biological Process for SPACA3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007342 fusion of sperm to egg plasma membrane involved in single fertilization IMP 15982649
GO:0009566 fertilization IEA --
GO:0035036 sperm-egg recognition IEA --
GO:0042117 monocyte activation TAS 11591365
GO:0043032 positive regulation of macrophage activation TAS 11591365
genes like me logo Genes that share ontologies with SPACA3: view

No data available for Pathways by source and SIGNOR curated interactions for SPACA3 Gene

Drugs & Compounds for SPACA3 Gene

No Compound Related Data Available

Transcripts for SPACA3 Gene

mRNA/cDNA for SPACA3 Gene

(3) REFSEQ mRNAs :
(7) Additional mRNA sequences :
(16) Selected AceView cDNA sequences:
(5) Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for SPACA3

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for SPACA3 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3a · 3b · 3c ^ 4a · 4b ^ 5 ^ 6
SP1: - -
SP2: - - - - -
SP3:
SP4: - -

Relevant External Links for SPACA3 Gene

GeneLoc Exon Structure for
SPACA3
ECgene alternative splicing isoforms for
SPACA3

Expression for SPACA3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for SPACA3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for SPACA3 Gene

This gene is overexpressed in Testis (x49.3).

Protein differential expression in normal tissues from HIPED for SPACA3 Gene

This gene is overexpressed in Ovary (32.9), Liver, secretome (17.7), Testis (10.1), and Spleen (8.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for SPACA3 Gene



Protein tissue co-expression partners for SPACA3 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of SPACA3 Gene:

SPACA3

SOURCE GeneReport for Unigene cluster for SPACA3 Gene:

Hs.434112

mRNA Expression by UniProt/SwissProt for SPACA3 Gene:

Q8IXA5-SACA3_HUMAN
Tissue specificity: The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.
genes like me logo Genes that share expression patterns with SPACA3: view

No data available for Evidence on tissue expression from TISSUES and Phenotype-based relationships between genes and organs from Gene ORGANizer for SPACA3 Gene

Orthologs for SPACA3 Gene

This gene was present in the common ancestor of animals.

Orthologs for SPACA3 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia SPACA3 32
  • 99.67 (n)
dog
(Canis familiaris)
Mammalia SPACA3 33 32
  • 83.64 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Spaca3 17 33 32
  • 81.8 (n)
cow
(Bos Taurus)
Mammalia SPACA3 33 32
  • 81.6 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Spaca3 32
  • 81.6 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia SPACA3 33
  • 42 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia SPACA3 33
  • 48 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta CG7798 33
  • 34 (a)
ManyToMany
CG11159 33
  • 32 (a)
ManyToMany
LysX 33
  • 32 (a)
ManyToMany
LysE 33
  • 31 (a)
ManyToMany
LysP 33
  • 31 (a)
ManyToMany
LysC 33
  • 31 (a)
ManyToMany
LysS 33
  • 31 (a)
ManyToMany
LysB 33
  • 31 (a)
ManyToMany
LysD 33
  • 31 (a)
ManyToMany
CG16756 33
  • 31 (a)
ManyToMany
CG30062 33
  • 29 (a)
ManyToMany
CG16799 33
  • 23 (a)
ManyToMany
CG8492 33
  • 4 (a)
ManyToMany
Species where no ortholog for SPACA3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)
  • zebrafish (Danio rerio)

Evolution for SPACA3 Gene

ENSEMBL:
Gene Tree for SPACA3 (if available)
TreeFam:
Gene Tree for SPACA3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for SPACA3: view image

Paralogs for SPACA3 Gene

Paralogs for SPACA3 Gene

(8) SIMAP similar genes for SPACA3 Gene using alignment to 3 proteins:

  • SACA3_HUMAN
  • E9PF91_HUMAN
  • Q05C28_HUMAN
genes like me logo Genes that share paralogs with SPACA3: view

Variants for SPACA3 Gene

Sequence variations from dbSNP and Humsavar for SPACA3 Gene

SNP ID Clin Chr 17 pos Variation AA Info Type
rs1000081754 -- 32,994,532(+) T/C intron_variant
rs1000543372 -- 32,997,068(+) T/A intron_variant
rs1001159677 -- 32,995,082(+) C/T intron_variant
rs1001583447 -- 32,997,596(+) C/T intron_variant
rs1001743826 -- 32,991,044(+) G/A upstream_transcript_variant

Structural Variations from Database of Genomic Variants (DGV) for SPACA3 Gene

Variant ID Type Subtype PubMed ID
nsv510705 CNV deletion 20534489
nsv833417 CNV gain 17160897

Variation tolerance for SPACA3 Gene

Residual Variation Intolerance Score: 87.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.90; 48.76% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for SPACA3 Gene

Human Gene Mutation Database (HGMD)
SPACA3
SNPedia medical, phenotypic, and genealogical associations of SNPs for
SPACA3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SPACA3 Gene

Disorders for SPACA3 Gene

Additional Disease Information for SPACA3

No disorders were found for SPACA3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for SPACA3 Gene

Publications for SPACA3 Gene

  1. SLLP1, a unique, intra-acrosomal, non-bacteriolytic, c lysozyme-like protein of human spermatozoa. (PMID: 12606493) Mandal A … Herr JC (Biology of reproduction 2003) 2 3 4 23 56
  2. Molecular cloning and characterization of three novel lysozyme-like genes, predominantly expressed in the male reproductive system of humans, belonging to the c-type lysozyme/alpha-lactalbumin family. (PMID: 16014814) Zhang K … Yu L (Biology of reproduction 2005) 3 4 23 56
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 56
  4. The spermatozoa protein, SLLP1, is a novel cancer-testis antigen in hematologic malignancies. (PMID: 15475442) Wang Z … Lim SH (Clinical cancer research : an official journal of the American Association for Cancer Research 2004) 3 23 56
  5. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PMID: 12975309) Clark HF … Gray A (Genome research 2003) 3 4 56

Products for SPACA3 Gene

Sources for SPACA3 Gene