SP1 Gene (Protein Coding)

Sp1 Transcription Factor

GC12P053380
GIFtS:
45
The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycos... See more...

Aliases for SP1 Gene

Aliases for SP1 Gene

  • Sp1 Transcription Factor 2 3 5
  • Transcription Factor Sp1 3 4
  • Specificity Protein 1 2 3
  • TSFP1 4

External Ids for SP1 Gene

Previous GeneCards Identifiers for SP1 Gene

  • GC12P053801
  • GC12P053490
  • GC12P052060
  • GC12P050814
  • GC12P053773

Summaries for SP1 Gene

Entrez Gene Summary for SP1 Gene

  • The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

GeneCards Summary for SP1 Gene

SP1 (Sp1 Transcription Factor) is a Protein Coding gene. Diseases associated with SP1 include Huntington Disease and Cytomegalovirus Infection. Among its related pathways are Direct p53 effectors and IL4-mediated signaling events. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and sequence-specific DNA binding. An important paralog of this gene is SP4.

UniProtKB/Swiss-Prot Summary for SP1 Gene

  • Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1 (PubMed:10391891, PubMed:11371615, PubMed:11904305, PubMed:14593115, PubMed:16377629, PubMed:16478997, PubMed:16943418, PubMed:17049555, PubMed:18171990, PubMed:18199680, PubMed:18239466, PubMed:18513490, PubMed:18619531, PubMed:19193796, PubMed:20091743, PubMed:21798247, PubMed:21046154). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity).

Gene Wiki entry for SP1 Gene

Additional gene information for SP1 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for SP1 Gene

Genomics for SP1 Gene

GeneHancer (GH) Regulatory Elements for SP1 Gene

Promoters and enhancers for SP1 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around SP1 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for SP1

Top Transcription factor binding sites by QIAGEN in the SP1 gene promoter:
  • AP-1
  • ATF-2
  • c-Jun
  • STAT1

Genomic Locations for SP1 Gene

Genomic Locations for SP1 Gene
chr12:53,380,176-53,416,446
(GRCh38/hg38)
Size:
36,271 bases
Orientation:
Plus strand
chr12:53,773,960-53,810,230
(GRCh37/hg19)
Size:
36,271 bases
Orientation:
Plus strand

Genomic View for SP1 Gene

Genes around SP1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
SP1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for SP1 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for SP1 Gene

Proteins for SP1 Gene

Products:

  1. Antibody
  2. Protein
  3. Assay

  • Protein details for SP1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P08047-SP1_HUMAN
    Recommended name:
    Transcription factor Sp1
    Protein Accession:
    P08047
    Secondary Accessions:
    • E4Z9M7
    • G5E9M8
    • Q86TN8
    • Q9H3Q5
    • Q9NR51
    • Q9NY21
    • Q9NYE7

    Protein attributes for SP1 Gene

    Size:
    785 amino acids
    Molecular mass:
    80693 Da
    Quaternary structure:
    • Interacts with ATF7IP, ATF7IP2, BAHD1, POGZ, HCFC1, AATF and PHC2. Interacts with HLTF; the interaction may be required for basal transcriptional activity of HLTF. Interacts (deacetylated form) with EP300; the interaction enhances gene expression. Interacts with HDAC1 and JUN. Interacts with ELF1; the interaction is inhibited by glycosylation of SP1. Interaction with NFYA; the interaction is inhibited by glycosylation of SP1. Interacts with ATF7IP and TBP. Interacts with MEIS2 isoform 4 and PBX1 isoform PBX1a. Interacts with EGR1 (PubMed:10391891, PubMed:10976766, PubMed:12021324, PubMed:12847090, PubMed:12855699, PubMed:15691849, PubMed:16478997, PubMed:19106100, PubMed:19285002, PubMed:19302979, PubMed:19666599, PubMed:20121949, PubMed:21746878, PubMed:7592727, PubMed:9466902). Interacts with SMARCA4/BRG1. Interacts with RNF112 in an oxidative stress-regulated manner (By similarity). Interacts with ZBTB7A; ZBTB7A prevents the binding to GC-rich motifs in promoters and represses the transcriptional activity of SP1 (PubMed:12004059).
    • (Microbial infection) Interacts with varicella-zoster virus IE62 protein.
    • (Microbial infection) Interacts with SV40 VP2/3 proteins. Interacts with SV40 major capsid protein VP1; this interaction leads to a cooperativity between the 2 proteins in DNA binding.
    • (Microbial infection) Interacts with HIV-1 Vpr; the interaction is inhibited by SP1 O-glycosylation.
    Miscellaneous:
    • In the hepatoma cell line Hep-G2, SP1 precursor mRNA may undergo homotype trans-splicing leading to the duplication of exons 2 and 3.
    SequenceCaution:
    • Sequence=AAH43224.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for SP1 Gene

    Alternative splice isoforms for SP1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SP1 Gene

Protein Expression for SP1 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Post-translational modifications for SP1 Gene

  • Phosphorylated on multiple serine and threonine residues. Phosphorylation is coupled to ubiquitination, sumoylation and proteolytic processing. Phosphorylation on Ser-59 enhances proteolytic cleavage. Phosphorylation on Ser-7 enhances ubiquitination and protein degradation. Hyperphosphorylation on Ser-101 in response to DNA damage has no effect on transcriptional activity. MAPK1/MAPK3-mediated phosphorylation on Thr-453 and Thr-739 enhances VEGF transcription but, represses FGF2-triggered PDGFR-alpha transcription. Also implicated in the repression of RECK by ERBB2. Hyperphosphorylated on Thr-278 and Thr-739 during mitosis by MAPK8 shielding SP1 from degradation by the ubiquitin-dependent pathway. Phosphorylated in the zinc-finger domain by calmodulin-activated PKCzeta. Phosphorylation on Ser-641 by PKCzeta is critical for TSA-activated LHR gene expression through release of its repressor, p107. Phosphorylation on Thr-668, Ser-670 and Thr-681 is stimulated by angiotensin II via the AT1 receptor inducing increased binding to the PDGF-D promoter. This phosphorylation is increased in injured artey wall. Ser-59 and Thr-681 can both be dephosphorylated by PP2A during cell-cycle interphase. Dephosphorylation on Ser-59 leads to increased chromatin association during interphase and increases the transcriptional activity. On insulin stimulation, sequentially glycosylated and phosphorylated on several C-terminal serine and threonine residues.
  • Acetylated. Acetylation/deacetylation events affect transcriptional activity. Deacetylation leads to an increase in the expression the 12(s)-lipooxygenase gene though recruitment of p300 to the promoter.
  • Ubiquitinated. Ubiquitination occurs on the C-terminal proteolytically-cleaved peptide and is triggered by phosphorylation.
  • Sumoylated with SUMO1. Sumoylation modulates proteolytic cleavage of the N-terminal repressor domain. Sumoylation levels are attenuated during tumorigenesis. Phosphorylation mediates SP1 desumoylation.
  • Proteolytic cleavage in the N-terminal repressor domain is prevented by sumoylation. The C-terminal cleaved product is susceptible to degradation.
  • O-glycosylated; Contains 8 N-acetylglucosamine side chains. Levels are controlled by insulin and the SP1 phosphorylation states. Insulin-mediated O-glycosylation locates SP1 to the nucleus, where it is sequentially deglycosylated and phosphorylated. O-glycosylation affects transcriptional activity through disrupting the interaction with a number of transcription factors including ELF1 and NFYA. Also inhibits interaction with the HIV1 promoter. Inhibited by peroxisomome proliferator receptor gamma (PPARgamma).
  • Glycosylation at Ser491, Ser612, Thr640, Ser641, Ser698, and Ser702
  • Modification sites at PhosphoSitePlus
  • Glycosylation from GlyConnect
    • SP1_HUMAN (605)

Other Protein References for SP1 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibody Products

  • Abcam antibodies for SP1

No data available for DME Specific Peptides for SP1 Gene

Domains & Families for SP1 Gene

Gene Families for SP1 Gene

HGNC:
Human Protein Atlas (HPA):
  • Cancer-related genes
  • Predicted intracellular proteins
  • Transcription factors

Protein Domains for SP1 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for SP1 Gene

GenScript: Design optimal peptide antigens:
  • cDNA, FLJ92269 (B2R4Y6_HUMAN)
  • Specificity protien 1 (C4PGM0_HUMAN)
  • Transcription factor Sp1 (E4Z9M7_HUMAN)
  • Pregnancy-specific protein (Q15403_HUMAN)
  • Transcription factor Sp1 (SP1_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P08047

UniProtKB/Swiss-Prot:

SP1_HUMAN :
  • The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Belongs to the Sp1 C2H2-type zinc-finger protein family.
Domain:
  • The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Family:
  • Belongs to the Sp1 C2H2-type zinc-finger protein family.
genes like me logo Genes that share domains with SP1: view

Function for SP1 Gene

Products:

  1. Animal Model
  2. CRISPR
  3. miRNA
  4. Inhibitory RNA
  5. Clone
  6. Cell Line

Molecular function for SP1 Gene

UniProtKB/Swiss-Prot Function:
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1 (PubMed:10391891, PubMed:11371615, PubMed:11904305, PubMed:14593115, PubMed:16377629, PubMed:16478997, PubMed:16943418, PubMed:17049555, PubMed:18171990, PubMed:18199680, PubMed:18239466, PubMed:18513490, PubMed:18619531, PubMed:19193796, PubMed:20091743, PubMed:21798247, PubMed:21046154). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity).
UniProtKB/Swiss-Prot Induction:
By insulin.
GENATLAS Biochemistry:
transcription factor Sp-1,GT box binding protein

Phenotypes From GWAS Catalog for SP1 Gene

Gene Ontology (GO) - Molecular Function for SP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000977 RNA polymerase II regulatory region sequence-specific DNA binding IDA 14979875
GO:0000978 RNA polymerase II proximal promoter sequence-specific DNA binding ISS,IDA --
GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific NAS 19274049
GO:0000987 proximal promoter sequence-specific DNA binding ISS 10362258
GO:0001103 RNA polymerase II repressing transcription factor binding ISS --
genes like me logo Genes that share ontologies with SP1: view
genes like me logo Genes that share phenotypes with SP1: view

Animal Models for SP1 Gene

MGI Knock Outs for SP1:
  • Sp1 Sp1<tm1Phi>

Animal Model Products

CRISPR Products

miRNA for SP1 Gene

miRTarBase miRNAs that target SP1

Transcription Factor Targets for SP1 Gene

Selected GeneGlobe predicted Target genes for SP1
Targeted motifs for SP1 Gene
HOMER Transcription Factor Regulatory Elements motif SP1
  • Consensus sequence: GGCCCCGCCCCC Submotif: canonical Cell Type: NA

Clone Products

  • Addgene plasmids for SP1

No data available for Enzyme Numbers (IUBMB) and Human Phenotype Ontology for SP1 Gene

Localization for SP1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SP1 Gene

Nucleus. Cytoplasm. Note=Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for SP1 gene
Compartment Confidence
nucleus 5
plasma membrane 2
extracellular 2
cytoskeleton 2
mitochondrion 2
peroxisome 2
endoplasmic reticulum 2
cytosol 2
lysosome 2
endosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoplasm (4)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for SP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000790 nuclear chromatin ISA --
GO:0005634 nucleus IC,IEA 3139301
GO:0005654 nucleoplasm TAS,IDA --
GO:0005737 cytoplasm IEA --
GO:0017053 transcriptional repressor complex IDA 16691198
genes like me logo Genes that share ontologies with SP1: view

Pathways & Interactions for SP1 Gene

genes like me logo Genes that share pathways with SP1: view

Pathways by source for SP1 Gene

1 PharmGKB pathway for SP1 Gene
15 GeneGo (Thomson Reuters) pathways for SP1 Gene
  • Cell adhesion Tight junctions
  • Cell cycle ESR1 regulation of G1/S transition
  • Cell cycle Regulation of G1/S transition (part 1)
  • Cell cycle Regulation of G1/S transition (part 2)
  • Development Endothelin-1/EDNRA transactivation of EGFR
11 Qiagen pathways for SP1 Gene
  • AHR Pathway
  • CMV and MAPK Pathways
  • ErbB2-ErbB3 Heterodimers
  • Estrogen Pathway
  • HMGB1 Pathway
2 Cell Signaling Technology pathways for SP1 Gene

SIGNOR curated interactions for SP1 Gene

Activates:
Inactivates:
Is activated by:
Is inactivated by:
Other effect:

Gene Ontology (GO) - Biological Process for SP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006355 regulation of transcription, DNA-templated IDA 12169688
GO:0006357 regulation of transcription by RNA polymerase II IBA,IEA 21873635
GO:0010628 positive regulation of gene expression IMP 23802567
GO:0016032 viral process IEA --
GO:0032869 cellular response to insulin stimulus IEA --
genes like me logo Genes that share ontologies with SP1: view

Drugs & Compounds for SP1 Gene

Products:

  1. Drug

(75) Drugs for SP1 Gene - From: PharmGKB, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Metformin Approved Pharma AMP-activated protein kinase (AMPK) activator 2154
Serotonin Investigational Nutra 4422

(55) Additional Compounds for SP1 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with SP1: view

Transcripts for SP1 Gene

Products:

  1. CRISPR
  2. miRNA
  3. Inhibitory RNA
  4. Clone

mRNA/cDNA for SP1 Gene

3 REFSEQ mRNAs :
24 NCBI additional mRNA sequence :
4 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products