SLC22A8 Gene (Protein Coding)

Solute Carrier Family 22 Member 8

GC11M063042
GIFtS:
41
This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for ... See more...

Aliases for SLC22A8 Gene

Aliases for SLC22A8 Gene

  • Solute Carrier Family 22 Member 8 2 3 4 5
  • Solute Carrier Family 22 (Organic Anion Transporter), Member 8 2 3
  • Organic Anion Transporter 3 3 4
  • HOAT3 3 4
  • OAT3 3 4

External Ids for SLC22A8 Gene

Previous GeneCards Identifiers for SLC22A8 Gene

  • GC11M065273
  • GC11M064335
  • GC11M063012
  • GC11M062536
  • GC11M062520
  • GC11M062516
  • GC11M062760
  • GC11M059089
  • GC11M063015

Summaries for SLC22A8 Gene

Entrez Gene Summary for SLC22A8 Gene

  • This gene encodes a protein involved in the sodium-independent transport and excretion of organic anions, some of which are potentially toxic. The encoded protein is an integral membrane protein and appears to be localized to the basolateral membrane of the kidney. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, May 2010]

GeneCards Summary for SLC22A8 Gene

SLC22A8 (Solute Carrier Family 22 Member 8) is a Protein Coding gene. Among its related pathways are Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds and Blood-Brain Barrier Pathway: Anatomy. Gene Ontology (GO) annotations related to this gene include transmembrane transporter activity and ion transmembrane transporter activity. An important paralog of this gene is SLC22A6.

UniProtKB/Swiss-Prot Summary for SLC22A8 Gene

  • Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Involved in the transport basolateral of steviol, fexofenadine. Transports benzylpenicillin (PCG), estrone-3-sulfate (E1S), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH), acyclovir (ACV) and ochratoxin (OTA).

Gene Wiki entry for SLC22A8 Gene

Additional gene information for SLC22A8 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for SLC22A8 Gene

Genomics for SLC22A8 Gene

GeneHancer (GH) Regulatory Elements for SLC22A8 Gene

Promoters and enhancers for SLC22A8 Gene
GeneHancer (GH) Identifier GH Type GH
Score		 GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites		 Gene Targets
GH11J063015 Promoter/Enhancer 0.7 EPDnew Ensembl 750.6 -0.2 -156 1.6 NR2F6 HLF SLC22A8 lnc-SLC22A8-2 ENSG00000256041
GH11J063017 Enhancer 0.4 Ensembl 750.6 -1.9 -1856 1 ZBTB8A PRDM10 RCOR1 SLC22A8 lnc-SLC22A8-2 ENSG00000256041
GH11J063245 Enhancer 0.8 dbSUPER 10.7 -231.7 -231695 3.3 NR2F6 CREB1 SP1 HNF1A SP5 ZNF384 ZNF644 CEBPA GATAD2A RXRB SLC22A8 SLC22A9 SLC22A25 LOC105369334 lnc-SLC22A25-1 SLC22A10
GH11J062896 Enhancer 0.4 Ensembl dbSUPER 11 +119.2 119244 0.4 NR2F2 SLC22A8 ROM1 HSALNG0084674 ENSG00000257002
GH11J062895 Enhancer 0.4 Ensembl dbSUPER 11 +120.0 120044 0.4 GLIS1 SLC22A8 TMEM223 HSALNG0084674 ENSG00000257002
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around SLC22A8 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the SLC22A8 gene promoter:
  • aMEF-2
  • CREB
  • deltaCREB

Genomic Locations for SLC22A8 Gene

Genomic Locations for SLC22A8 Gene
chr11:62,989,154-63,015,845
(GRCh38/hg38)
Size:
26,692 bases
Orientation:
Minus strand
chr11:62,756,626-62,783,317
(GRCh37/hg19)
Size:
26,692 bases
Orientation:
Minus strand

Genomic View for SLC22A8 Gene

Genes around SLC22A8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
SLC22A8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for SLC22A8 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for SLC22A8 Gene

Proteins for SLC22A8 Gene

Products:

  1. Antibody
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  3. Assay

  • Protein details for SLC22A8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8TCC7-S22A8_HUMAN
    Recommended name:
    Solute carrier family 22 member 8
    Protein Accession:
    Q8TCC7
    Secondary Accessions:
    • B4DPH7
    • F5GWA8
    • F5H5J1
    • O95820
    • Q59EW9
    • Q96TC1

    Protein attributes for SLC22A8 Gene

    Size:
    542 amino acids
    Molecular mass:
    59856 Da
    Quaternary structure:
    No Data Available
    SequenceCaution:
    • Sequence=AAD19357.1; Type=Frameshift; Positions=Several; Evidence={ECO:0000305}; Sequence=BAD92929.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for SLC22A8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SLC22A8 Gene

Protein Expression for SLC22A8 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Post-translational modifications for SLC22A8 Gene

  • Glycosylation at Asn102 and Asn86
  • Modification sites at PhosphoSitePlus

Other Protein References for SLC22A8 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for SLC22A8 Gene

Domains & Families for SLC22A8 Gene

Gene Families for SLC22A8 Gene

HGNC:
IUPHAR :
Human Protein Atlas (HPA):
  • FDA approved drug targets
  • Predicted membrane proteins
  • Transporters

Protein Domains for SLC22A8 Gene

Blocks:
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for SLC22A8 Gene

GenScript: Design optimal peptide antigens:
  • Organic anion transporter 3 (S22A8_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q8TCC7

UniProtKB/Swiss-Prot:

S22A8_HUMAN :
  • Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.
Family:
  • Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.
genes like me logo Genes that share domains with SLC22A8: view

Function for SLC22A8 Gene

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  6. Cell Line

Molecular function for SLC22A8 Gene

UniProtKB/Swiss-Prot Function:
Plays an important role in the excretion/detoxification of endogenous and exogenous organic anions, especially from the brain and kidney. Involved in the transport basolateral of steviol, fexofenadine. Transports benzylpenicillin (PCG), estrone-3-sulfate (E1S), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), p-amino-hippurate (PAH), acyclovir (ACV) and ochratoxin (OTA).
GENATLAS Biochemistry:
solute carrier protein family 22,member A8,polyspecific transporter for organic cations,mediating the coupled exchange of alpha-ketoglutarate with multiple organic ions,expressed strongly in kidney,weakly in brain

Phenotypes From GWAS Catalog for SLC22A8 Gene

Gene Ontology (GO) - Molecular Function for SLC22A8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005452 inorganic anion exchanger activity IDA 15037815
GO:0022857 transmembrane transporter activity IEA --
genes like me logo Genes that share ontologies with SLC22A8: view
genes like me logo Genes that share phenotypes with SLC22A8: view

Animal Models for SLC22A8 Gene

MGI Knock Outs for SLC22A8:

Animal Model Products

CRISPR Products

miRNA for SLC22A8 Gene

miRTarBase miRNAs that target SLC22A8

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for SLC22A8

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for SLC22A8 Gene

Localization for SLC22A8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for SLC22A8 Gene

Basolateral cell membrane; Multi-pass membrane protein. Note=Localizes on the brush border membrane of the choroid epithelial cells. Localizes to the basolateral membrane of the proximal tubular cells. Localizes on the abluminal and possibly, luminal membrane of the brain capillary endothelial cells (BCEC) (By similarity). {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for SLC22A8 gene
Compartment Confidence
plasma membrane 5
extracellular 1
mitochondrion 1
nucleus 1

Gene Ontology (GO) - Cellular Components for SLC22A8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005886 plasma membrane IBA,TAS --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
GO:0016323 basolateral plasma membrane IEA,IDA 15037815
GO:0070062 extracellular exosome HDA 19056867
genes like me logo Genes that share ontologies with SLC22A8: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for SLC22A8 Gene

Pathways & Interactions for SLC22A8 Gene

genes like me logo Genes that share pathways with SLC22A8: view

Interacting Proteins for SLC22A8 Gene

STRING Interaction Network Preview (showing top 5 STRING interactants - click image to see top 25)
STRING Interaction Network
Selected Interacting proteins: ENSP00000337335 Q8TCC7-S22A8_HUMAN for SLC22A8 Gene via STRING IID

GPS-Prot Interaction Network for SLC22A8

Gene Ontology (GO) - Biological Process for SLC22A8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006811 ion transport IEA --
GO:0009636 response to toxic substance IEA --
GO:0015698 inorganic anion transport IEA --
GO:0055085 transmembrane transport IEA --
genes like me logo Genes that share ontologies with SLC22A8: view

No data available for SIGNOR curated interactions for SLC22A8 Gene

Drugs & Compounds for SLC22A8 Gene

Products:

  1. Drug

(134) Drugs for SLC22A8 Gene - From: DrugBank, PharmGKB, DGIdb, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Probenecid Approved, Investigational Pharma Inhibition, Inhibitor, Activator, Target, Transporter, inhibitor, blocker 40
Cefotaxime Approved Pharma Target, Transporter, inhibitor 45
Dabrafenib Approved, Investigational Pharma Transporter, inhibitor BRAF kinase inhibitor, Kinase Inhibitors, Mutant BRAF Kinase 108
Dolutegravir Approved Pharma Transporter, inhibitor 215
Rucaparib Approved, Investigational Pharma Transporter, inhibitor PARP inhibitor, PARP Inhibitors, Other 48

(5) Additional Compounds for SLC22A8 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with SLC22A8: view

Transcripts for SLC22A8 Gene

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mRNA/cDNA for SLC22A8 Gene

(4) REFSEQ mRNAs :
(12) Additional mRNA sequences :
(46) Selected AceView cDNA sequences:
(10) Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for SLC22A8

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for SLC22A8 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6a · 6b · 6c · 6d ^ 7a · 7b · 7c ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12a · 12b · 12c
SP1: - - - -
SP2: - - - -
SP3: - -
SP4: - - - - -
SP5: -
SP6: - - -
SP7: - - - - - -
SP8:
SP9:

Relevant External Links for SLC22A8 Gene

GeneLoc Exon Structure for
SLC22A8
ECgene alternative splicing isoforms for
SLC22A8

Expression for SLC22A8 Gene

Products:

  1. Primer
  2. Gene Expression Assay

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for SLC22A8 Gene

mRNA expression in normal human tissues for SLC22A8 Gene
mRNA expression by GTEx for SLC22A8 GenemRNA expression by BioGPS for SLC22A8 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for SLC22A8 Gene

This gene is overexpressed in Kidney - Cortex (x51.3).

Protein differential expression in normal tissues from HIPED for SLC22A8 Gene

This gene is overexpressed in Kidney (42.5), Urine (13.2), and Heart (9.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for SLC22A8 Gene



Protein tissue co-expression partners for SLC22A8 Gene

NURSA nuclear receptor signaling pathways regulating expression of SLC22A8 Gene:

SLC22A8

SOURCE GeneReport for Unigene cluster for SLC22A8 Gene:

Hs.266223

mRNA Expression by UniProt/SwissProt for SLC22A8 Gene:

Q8TCC7-S22A8_HUMAN
Tissue specificity: Expressed in kidney.

Evidence on tissue expression from TISSUES for SLC22A8 Gene

  • Kidney(4.9)
  • Nervous system(4.4)
  • Blood(2.2)
  • Urine(2.1)
genes like me logo Genes that share expression patterns with SLC22A8: view

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for SLC22A8 Gene

Orthologs for SLC22A8 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for SLC22A8 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
 Mammalia SLC22A8 33 32
  • 95.28 (n)
 OneToOne
cow
(Bos Taurus)
 Mammalia SLC22A8 32
  • 85.24 (n)
OAT3 33
  • 82 (a)
 OneToOne
dog
(Canis familiaris)
 Mammalia SLC22A8 33 32
  • 85.01 (n)
 OneToOne
rat
(Rattus norvegicus)
 Mammalia Slc22a8 32
  • 83.02 (n)
mouse
(Mus musculus)
 Mammalia Slc22a8 17 33 32
  • 82.18 (n)
platypus
(Ornithorhynchus anatinus)
 Mammalia SLC22A8 33
  • 62 (a)
 OneToOne
oppossum
(Monodelphis domestica)
 Mammalia -- 33
  • 39 (a)
 ManyToMany
lizard
(Anolis carolinensis)
 Reptilia -- 33
  • 50 (a)
 ManyToMany
-- 33
  • 50 (a)
 ManyToMany
-- 33
  • 49 (a)
 ManyToMany
-- 33
  • 46 (a)
 ManyToMany
zebrafish
(Danio rerio)
 Actinopterygii slc22a6l 33
  • 47 (a)
 OneToMany
baker's yeast
(Saccharomyces cerevisiae)
 Saccharomycetes MPH3 35
Species where no ortholog for SLC22A8 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for SLC22A8 Gene

ENSEMBL:
Gene Tree for SLC22A8 (if available)
TreeFam:
Gene Tree for SLC22A8 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for SLC22A8: view image

Paralogs for SLC22A8 Gene

Paralogs for SLC22A8 Gene

(21) SIMAP similar genes for SLC22A8 Gene using alignment to 2 proteins:

  • S22A8_HUMAN
  • H7BXN9_HUMAN
genes like me logo Genes that share paralogs with SLC22A8: view

Variants for SLC22A8 Gene

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Sequence variations from dbSNP and Humsavar for SLC22A8 Gene

SNP ID Clin Chr 11 pos Variation AA Info Type
rs1000192624 -- 63,005,576(-) T/C genic_upstream_transcript_variant, intron_variant
rs1000234719 -- 63,017,156(-) T/C upstream_transcript_variant
rs1000242749 -- 63,010,530(-) C/A genic_upstream_transcript_variant, intron_variant
rs1000358390 -- 63,010,288(-) C/T genic_upstream_transcript_variant, intron_variant
rs1000396503 -- 63,010,800(-) C/T genic_upstream_transcript_variant, intron_variant

Structural Variations from Database of Genomic Variants (DGV) for SLC22A8 Gene

Variant ID Type Subtype PubMed ID
dgv204e214 CNV gain 21293372
esv34011 CNV loss 18971310

Variation tolerance for SLC22A8 Gene

Residual Variation Intolerance Score: 49.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.60; 56.35% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for SLC22A8 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
SLC22A8

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SLC22A8 Gene

Disorders for SLC22A8 Gene

Additional Disease Information for SLC22A8

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for SLC22A8 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for SLC22A8 Gene

Publications for SLC22A8 Gene

  1. The human organic anion transporter 3 (OAT3; SLC22A8): genetic variation and functional genomics. (PMID: 16291576) Erdman AR … Giacomini KM (American journal of physiology. Renal physiology 2006) 3 4 23 43 56
  2. Polymorphisms of OATP-C (SLC21A6) and OAT3 (SLC22A8) genes: consequences for pravastatin pharmacokinetics. (PMID: 12811365) Nishizato Y … Sugiyama Y (Clinical pharmacology and therapeutics 2003) 3 4 23 43 56
  3. Inhibition of oat3-mediated renal uptake as a mechanism for drug-drug interaction between fexofenadine and probenecid. (PMID: 16455804) Tahara H … Sugiyama Y (Drug metabolism and disposition: the biological fate of chemicals 2006) 3 4 23 56
  4. Molecular cloning and functional analyses of OAT1 and OAT3 from cynomolgus monkey kidney. (PMID: 15846473) Tahara H … Sugiyama Y (Pharmaceutical research 2005) 3 4 23 56
  5. Human organic anion transporter 3 (hOAT3) can operate as an exchanger and mediate secretory urate flux. (PMID: 14586168) Bakhiya A … Wolff N (Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2003) 3 4 23 56

ExternalLinks

View latest publications for SLC22A8 gene in Mastermind

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