SHFL Gene (Protein Coding)

Shiftless Antiviral Inhibitor Of Ribosomal Frameshifting

GC19P010086
GIFtS:
25
See more...

Aliases for SHFL Gene

Aliases for SHFL Gene

  • Shiftless Antiviral Inhibitor Of Ribosomal Frameshifting 2 3 5
  • RyDEN 2 3 4
  • IRAV 2 3 4
  • SFL 2 3 4
  • Shiftless Antiviral Inhibitor Of Ribosomal Frameshifting Protein 3 4
  • Interferon-Regulated Antiviral Protein 3 4
  • Repressor Of Yield Of Dengue Virus 2 3
  • Repressor Of Yield Of DENV Protein 3 4
  • Shiftless 2 3
  • C19orf66 3 4
  • FLJ11286 2 4
  • Interferon-Regulated Antiviral Gene 2
  • Chromosome 19 Open Reading Frame 66 2
  • Interferon-Regulated Antiviral 3
  • UPF0515 Protein C19orf66 3
  • RYDEN 4
  • SHFL 5

External Ids for SHFL Gene

Previous HGNC Symbols for SHFL Gene

  • C19orf66

Summaries for SHFL Gene

GeneCards Summary for SHFL Gene

SHFL (Shiftless Antiviral Inhibitor Of Ribosomal Frameshifting) is a Protein Coding gene. Diseases associated with SHFL include Dengue Virus and Chikungunya.

UniProtKB/Swiss-Prot Summary for SHFL Gene

  • Inhibits programmed -1 ribosomal frameshifting (-1PRF) of a variety of mRNAs from viruses, such as HIV1, and cellular genes, such as PEG10. Interacts with the -1PRF signal of target mRNA and translating ribosomes and causes premature translation termination at the frameshifting site (PubMed:30682371). Regulates HIV1 GAG-POL expression by inhibiting -1PRF (PubMed:30682371). Exhibits antiviral activity against dengue virus (DENV) and can inhibit the replication of all DENV serotypes. May block the protein translation of DENV RNA via its association with cellular mRNA-binding proteins and viral RNA. Can also limit the replication of hepatitis C virus (HCV), West Nile virus (WNV), Chikungunya virus (CHIKV), herpes simplex virus type 1 (HHV-1) and human adenovirus (PubMed:26735137, PubMed:27974568). Binds nucleic acids with a higher affinity for ssRNA and ssDNA than for dsDNA (PubMed:27974568).
  • Isoform 4 does not inhibit programmed ribosomal frameshifting (-1PRF). Does not bind to ribosomes.

Additional gene information for SHFL Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for SHFL Gene

Genomics for SHFL Gene

GeneHancer (GH) Regulatory Elements Pubs

Download GeneHancer 2017 data
Promoters and enhancers for SHFL Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around SHFL on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for SHFL

Genomic Locations for SHFL Gene

Genomic Locations for SHFL Gene
chr19:10,086,119-10,093,252
(GRCh38/hg38)
Size:
7,134 bases
Orientation:
Plus strand
chr19:10,196,798-10,203,928
(GRCh37/hg19)
Size:
7,131 bases
Orientation:
Plus strand

Genomic View for SHFL Gene

Genes around SHFL on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
SHFL Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for SHFL Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for SHFL Gene

Proteins for SHFL Gene

Products:

  1. Antibody
  2. Protein
  3. Assay

  • Protein details for SHFL Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NUL5-SHFL_HUMAN
    Recommended name:
    Shiftless antiviral inhibitor of ribosomal frameshifting protein
    Protein Accession:
    Q9NUL5
    Secondary Accessions:
    • A8MQT9
    • Q4G188
    • Q8IYH6
    • Q8N8V1

    Protein attributes for SHFL Gene

    Size:
    291 amino acids
    Molecular mass:
    33110 Da
    Quaternary structure:
    • Interacts with PABPC1 (PubMed:26735137). Found in a complex with PABPC1 and LARP1 (PubMed:26735137). Interacts with ELAV1, MOV10 and UPF1; the interactions increase in presence of RNA (PubMed:27974568). Binds to ribosomes (PubMed:30682371). Interacts with GSPT1 (PubMed:30682371).
    • (Microbial infection) Interacts with the human dengue virus DENV proteins NS3 and NS4A.

    Alternative splice isoforms for SHFL Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SHFL Gene

Post-translational modifications for SHFL Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for SHFL Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for SHFL Gene

Domains & Families for SHFL Gene

Gene Families for SHFL Gene

Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for SHFL Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for SHFL Gene

GenScript: Design optimal peptide antigens:
  • UPF0515 protein C19orf66 (CS066_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9NUL5

UniProtKB/Swiss-Prot:

SHFL_HUMAN :
  • Belongs to the SHFL family.
Family:
  • Belongs to the SHFL family.
genes like me logo Genes that share domains with SHFL: view

Function for SHFL Gene

Products:

  1. Animal Model
  2. CRISPR
  3. miRNA
  4. Inhibitory RNA
  5. Clone
  6. Cell Line

Molecular function for SHFL Gene

UniProtKB/Swiss-Prot Function:
Inhibits programmed -1 ribosomal frameshifting (-1PRF) of a variety of mRNAs from viruses, such as HIV1, and cellular genes, such as PEG10. Interacts with the -1PRF signal of target mRNA and translating ribosomes and causes premature translation termination at the frameshifting site (PubMed:30682371). Regulates HIV1 GAG-POL expression by inhibiting -1PRF (PubMed:30682371). Exhibits antiviral activity against dengue virus (DENV) and can inhibit the replication of all DENV serotypes. May block the protein translation of DENV RNA via its association with cellular mRNA-binding proteins and viral RNA. Can also limit the replication of hepatitis C virus (HCV), West Nile virus (WNV), Chikungunya virus (CHIKV), herpes simplex virus type 1 (HHV-1) and human adenovirus (PubMed:26735137, PubMed:27974568). Binds nucleic acids with a higher affinity for ssRNA and ssDNA than for dsDNA (PubMed:27974568).
UniProtKB/Swiss-Prot Function:
Isoform 4 does not inhibit programmed ribosomal frameshifting (-1PRF). Does not bind to ribosomes.
UniProtKB/Swiss-Prot Induction:
Up-regulated by interferon (IFN) treatment (PubMed:26735137, PubMed:27974568, PubMed:30682371). Expression increases in response to DENV infection in an IFN-dependent manner (PubMed:27974568).

Phenotypes From GWAS Catalog for SHFL Gene

Gene Ontology (GO) - Molecular Function for SHFL Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003723 RNA binding IEA,IDA 26735137
GO:0005515 protein binding IPI 21044950
GO:0043022 ribosome binding IDA 30682371
GO:1990825 sequence-specific mRNA binding IDA 30682371
genes like me logo Genes that share ontologies with SHFL: view
genes like me logo Genes that share phenotypes with SHFL: view

Animal Models for SHFL Gene

MGI Knock Outs for SHFL:
  • Shfl Shfl<tm1.1(KOMP)Mbp>

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for SHFL

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for SHFL Gene

Localization for SHFL Gene

Subcellular locations from UniProtKB/Swiss-Prot for SHFL Gene

Cytoplasm. Nucleus. Cytoplasm, P-body. Note=Predominantly found in the cytoplasm (PubMed:26735137). After infection, relocalizes to the DENV replication complex in perinuclear regions (PubMed:27974568). {ECO:0000269 PubMed:26735137, ECO:0000269 PubMed:27974568}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for SHFL gene
Compartment Confidence
nucleus 5
cytosol 5
extracellular 1
cytoskeleton 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (3)
  • Nucleoplasm (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for SHFL Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000932 P-body IEA,IDA 27974568
GO:0005634 nucleus IEA,IDA 26735137
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IEA,IDA 26735137
GO:0005829 cytosol IDA --
genes like me logo Genes that share ontologies with SHFL: view

Pathways & Interactions for SHFL Gene

PathCards logo

SuperPathways for SHFL Gene

No Data Available

Gene Ontology (GO) - Biological Process for SHFL Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006449 regulation of translational termination IDA 30682371
GO:0034340 response to type I interferon IDA 26735137
GO:0034341 response to interferon-gamma IDA 26735137
GO:0034342 response to type III interferon IDA 26735137
GO:0035456 response to interferon-beta IDA 27974568
genes like me logo Genes that share ontologies with SHFL: view

No data available for Pathways by source and SIGNOR curated interactions for SHFL Gene

Drugs & Compounds for SHFL Gene

Products:

  1. Drug
No Compound Related Data Available

Transcripts for SHFL Gene

Products:

  1. CRISPR
  2. miRNA
  3. Inhibitory RNA
  4. Clone

mRNA/cDNA for SHFL Gene

2 REFSEQ mRNAs :
10 NCBI additional mRNA sequence :
11 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for SHFL

Alternative Splicing Database (ASD) splice patterns (SP) for SHFL Gene

No ASD Table

Relevant External Links for SHFL Gene

GeneLoc Exon Structure for
SHFL

Expression for SHFL Gene

Products:

  1. Primer

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for SHFL

SOURCE GeneReport for Unigene cluster for SHFL Gene:

Hs.655613

Evidence on tissue expression from TISSUES for SHFL Gene

  • Liver(4.6)
  • Pancreas(4.1)
  • Nervous system(2.3)
genes like me logo Genes that share expression patterns with SHFL: view

Primer Products

No data available for mRNA expression in normal human tissues , mRNA differential expression in normal tissues , Protein differential expression in normal tissues , Protein expression , Protein tissue co-expression partners , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for SHFL Gene

Orthologs for SHFL Gene

This gene was present in the common ancestor of chordates.

Orthologs for SHFL Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
 Mammalia C19ORF66 31
  • 100 (a)
 OneToOne
C19H19orf66 30
  • 97.14 (n)
Dog
(Canis familiaris)
 Mammalia C19orf66 31
  • 95 (a)
 OneToOne
C20H19orf66 30
  • 90.96 (n)
Cow
(Bos Taurus)
 Mammalia C7H19orf66 30 31
  • 91.21 (n)
 OneToOne
Rat
(Rattus norvegicus)
 Mammalia LOC500956 30
  • 85.28 (n)
Mouse
(Mus musculus)
 Mammalia A230050P20Rik 30 31
  • 84.94 (n)
 OneToOne
Shfl 17
Oppossum
(Monodelphis domestica)
 Mammalia C19orf66 31
  • 57 (a)
 OneToOne
Lizard
(Anolis carolinensis)
 Reptilia C19orf66 31
  • 52 (a)
 OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
 Amphibia c19orf66 30
  • 61.22 (n)
Zebrafish
(Danio rerio)
 Actinopterygii zgc:171711 30
  • 58.24 (n)
C3H19orf66 31
  • 46 (a)
 OneToOne
Species where no ortholog for SHFL was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for SHFL Gene

ENSEMBL:
Gene Tree for SHFL (if available)
TreeFam:
Gene Tree for SHFL (if available)

Paralogs for SHFL Gene

No data available for Paralogs for SHFL Gene

Variants for SHFL Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for SHFL Gene

SNP ID Clinical significance and condition Chr 19 pos Variation AA Info Type
801417 Uncertain Significance: not provided 10,140,837(+) C/CGCAGCTTGG INFRAME_INSERTION
801418 Likely Pathogenic: not provided 10,154,930(+) T/C MISSENSE_VARIANT

Additional dbSNP identifiers (rs#s) for SHFL Gene

Structural Variations from Database of Genomic Variants (DGV) for SHFL Gene

Variant ID Type Subtype PubMed ID
nsv2414 CNV insertion 18451855
nsv458355 CNV loss 19166990
nsv578606 CNV loss 21841781
nsv833743 CNV loss 17160897

Variation tolerance for SHFL Gene

Residual Variation Intolerance Score: 57.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.63; 13.52% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for SHFL Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
SHFL

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SHFL Gene

Disorders for SHFL Gene

MalaCards: The human disease database

(2) MalaCards diseases for SHFL Gene - From: DISEASES and GeneCards

Disorder Aliases PubMed IDs
dengue virus
  • dengue virus, susceptibility to
chikungunya
  • chikungunya fever
- elite association - COSMIC cancer census association via MalaCards
Search SHFL in MalaCards View complete list of genes associated with diseases

Additional Disease Information for SHFL

ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with SHFL: view

No data available for UniProtKB/Swiss-Prot and Genatlas for SHFL Gene

Publications for SHFL Gene

  1. Regulation of HIV-1 Gag-Pol Expression by Shiftless, an Inhibitor of Programmed -1 Ribosomal Frameshifting. (PMID: 30682371) Wang X … Gao G (Cell 2019) 2 3 4
  2. IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10. (PMID: 27974568) Balinsky CA … Zoon KC (Journal of virology 2017) 2 3 4
  3. Characterization of RyDEN (C19orf66) as an Interferon-Stimulated Cellular Inhibitor against Dengue Virus Replication. (PMID: 26735137) Suzuki Y … Yamamoto N (PLoS pathogens 2016) 2 3 4
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4
  5. A reference map of the human binary protein interactome. (PMID: 32296183) Luck K … Calderwood MA (Nature 2020) 3

No data available for Mastermind for SHFL Gene

Products for SHFL Gene

Sources for SHFL Gene