This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alte... See more...

Aliases for SCRIB Gene

Aliases for SCRIB Gene

  • Scribble Planar Cell Polarity Protein 2 3 5
  • Protein Scribble Homolog 3 4
  • CRIB1 3 4
  • SCRB1 3 4
  • Scribbled Planar Cell Polarity Protein 3
  • Scribbled Homolog (Drosophila) 2
  • Scribbled Homolog 3
  • Protein LAP4 4
  • KIAA0147 4
  • Scribble 4
  • SCRIB1 3
  • Vartul 3
  • VARTUL 4
  • HScrib 4
  • LAP4 4

External Ids for SCRIB Gene

Previous GeneCards Identifiers for SCRIB Gene

  • GC08U990087
  • GC08M145136
  • GC08M144648
  • GC08M144979
  • GC08M144945
  • GC08M144874
  • GC08M140127
  • GC08M143791

Summaries for SCRIB Gene

Entrez Gene Summary for SCRIB Gene

  • This gene encodes a protein that was identified as being similar to the Drosophila scribble protein. The mammalian protein is involved in tumor suppression pathways. As a scaffold protein involved in cell polarization processes, this protein binds to many other proteins. The encoded protein binds to papillomavirus E6 protein via its PDZ domain and the C-terminus of E6. Two alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

GeneCards Summary for SCRIB Gene

SCRIB (Scribble Planar Cell Polarity Protein) is a Protein Coding gene. Diseases associated with SCRIB include Neural Tube Defects and Tick-Borne Encephalitis. Among its related pathways are Tight junction and Cytoskeletal Signaling. An important paralog of this gene is LRRC7.

UniProtKB/Swiss-Prot Summary for SCRIB Gene

  • Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.

Gene Wiki entry for SCRIB Gene

Additional gene information for SCRIB Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for SCRIB Gene

Genomics for SCRIB Gene

GeneHancer (GH) Regulatory Elements for SCRIB Gene

Promoters and enhancers for SCRIB Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH08J143814 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 757.3 +0.4 401 2.7 PPP1R10 ZNF24 SP1 CTCF SIX5 POLR2A HCFC1 MYC ELF1 L3MBTL2 SCRIB AB372758 VPS28 CPSF1 ZNF623 LRRC14 ZNF251 ADCK5 HSF1 PARP10
GH08J143902 Enhancer 1.2 Ensembl ENCODE dbSUPER 11.5 -88.1 -88117 3.2 CTCF MLX RBPJ CC2D1A L3MBTL2 RAD21 MNT MYC POLR2A KLF14 lnc-PLEC-4 PLEC MAF1 EPPK1 PUF60 SHARPIN MIR661 NRBP2 SCRIB PARP10
GH08J143905 Enhancer 1.2 Ensembl ENCODE dbSUPER 11.5 -90.8 -90770 2.1 RBPJ CREB1 MNT ELF3 TFE3 MYC POLR2A SP5 ZNF384 KAT8 PLEC ZNF7 ZNF250 VPS28 CPSF1 FBXL6 COMMD5 EPPK1 ADCK5 LRRC14
GH08J143836 Enhancer 0.8 Ensembl ENCODE 12.3 -20.8 -20807 1.2 RAD21 ZNF148 CTCF SP1 RXRA ZNF740 CHD2 EGR1 SMC3 SAP130 MIR6845 SCRIB EPPK1 MIR661 PLEC SPATC1 PARP10 FAM83H PUF60 NAPRT
GH08J143669 Enhancer 0.9 Ensembl 10.8 +146.2 146242 0.9 CTBP1 ZNF121 MTA3 NFIB SOX6 ZNF280D ZNF473 NCOA3 CBFA2T2 TRIM24 ZNF623 CPSF1 ZNF696 JRK LRRC14 VPS28 ZFP41 ZNF707 NAPRT ENSG00000255182
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around SCRIB on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the SCRIB gene promoter:
  • c-Ets-1
  • E2F
  • E2F-1
  • E2F-2
  • E2F-3a
  • E2F-4
  • E2F-5
  • FOXF2
  • HTF
  • YY1

Genomic Locations for SCRIB Gene

Genomic Locations for SCRIB Gene
chr8:143,790,920-143,815,773
(GRCh38/hg38)
Size:
24,854 bases
Orientation:
Minus strand
chr8:144,873,090-144,897,549
(GRCh37/hg19)
Size:
24,460 bases
Orientation:
Minus strand

Genomic View for SCRIB Gene

Genes around SCRIB on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
SCRIB Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for SCRIB Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for SCRIB Gene

Proteins for SCRIB Gene

  • Protein details for SCRIB Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q14160-SCRIB_HUMAN
    Recommended name:
    Protein scribble homolog
    Protein Accession:
    Q14160
    Secondary Accessions:
    • Q6P496
    • Q7Z5D1
    • Q8WWV8
    • Q96C69
    • Q96GG1

    Protein attributes for SCRIB Gene

    Size:
    1630 amino acids
    Molecular mass:
    174885 Da
    Quaternary structure:
    • Interacts (via PDZ domains) with VANGL2. Interacts with CTNNB1 and MAPK12 (By similarity). Interacts with UBE3A and HPV E6. Interacts with PAK1 and PAK2. Interacts with ARHGEF7 and GIT1; interacts directly with ARHGEF7. Interacts (via PDZ domains) with LPP and TRIP6; the interaction is direct. Interacts (via PDZ domains) with TJP2. Interacts (via PDZ domains) with APC; may mediate APC targeting to adherens junctions of epithelial cells. Interacts (via PDZ domains) with TSHR; regulates TSHR trafficking and function. Interacts (via PDZ domains 1 and 3) with MCC. Interacts (via fourth PDZ domain) with tick-borne encephalitis virus NS5 protein; this interaction inhibits SCRIB and downstream STAT1 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway. Interacts with DLG5 (PubMed:28169360). Interacts with STK4/MST1 and LATS1 in the presence of DLG5 (PubMed:28169360).
    • (Microbial infection) Interacts with tick-borne encephalitis virus RNA-directed RNA polymerase NS5; this interaction targets viral NS5 to the cell membrane periphery and nucleus, thereby allowing efficient host nuclear STAT1 inhibition.

    Three dimensional structures from OCA and Proteopedia for SCRIB Gene

    Alternative splice isoforms for SCRIB Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for SCRIB Gene

Post-translational modifications for SCRIB Gene

  • Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.
  • Ubiquitination at Lys53, Lys164, Lys266, and Lys272
  • Modification sites at PhosphoSitePlus

Other Protein References for SCRIB Gene

No data available for DME Specific Peptides for SCRIB Gene

Domains & Families for SCRIB Gene

Gene Families for SCRIB Gene

HGNC:
Human Protein Atlas (HPA):
  • Disease related genes
  • Predicted intracellular proteins

Protein Domains for SCRIB Gene

Suggested Antigen Peptide Sequences for SCRIB Gene

GenScript: Design optimal peptide antigens:
  • SCRIB protein (A0PJK8_HUMAN)
  • Protein LAP4 (SCRIB_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q14160

UniProtKB/Swiss-Prot:

SCRIB_HUMAN :
  • Belongs to the LAP (LRR and PDZ) protein family.
Family:
  • Belongs to the LAP (LRR and PDZ) protein family.
genes like me logo Genes that share domains with SCRIB: view

Function for SCRIB Gene

Molecular function for SCRIB Gene

UniProtKB/Swiss-Prot Function:
Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.

Phenotypes From GWAS Catalog for SCRIB Gene

Gene Ontology (GO) - Molecular Function for SCRIB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI,IEA 15182672
GO:0035255 ionotropic glutamate receptor binding IBA 21873635
GO:0045296 cadherin binding HDA 25468996
genes like me logo Genes that share ontologies with SCRIB: view
genes like me logo Genes that share phenotypes with SCRIB: view

Animal Models for SCRIB Gene

MGI Knock Outs for SCRIB:

Animal Model Products

CRISPR Products

Clone Products

  • Addgene plasmids for SCRIB

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for SCRIB Gene

Localization for SCRIB Gene

Subcellular locations from UniProtKB/Swiss-Prot for SCRIB Gene

Cell membrane; Peripheral membrane protein. Cell junction, adherens junction. Cell projection, lamellipodium. Cytoplasm. Note=Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for SCRIB gene
Compartment Confidence
plasma membrane 5
nucleus 5
cytosol 3
cytoskeleton 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cell Junctions (4)
  • Nucleoplasm (4)
  • Plasma membrane (4)
  • Rods & Rings (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for SCRIB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IEA --
GO:0005886 colocalizes_with plasma membrane IDA,IEA 16611247
GO:0005911 cell-cell junction IDA 15649318
GO:0005912 adherens junction IEA --
genes like me logo Genes that share ontologies with SCRIB: view

Pathways & Interactions for SCRIB Gene

genes like me logo Genes that share pathways with SCRIB: view

Gene Ontology (GO) - Biological Process for SCRIB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001843 neural tube closure IMP 22095531
GO:0001921 positive regulation of receptor recycling IMP 15775968
GO:0002093 auditory receptor cell morphogenesis IEA --
GO:0007275 multicellular organism development IEA --
GO:0008104 protein localization IEA --
genes like me logo Genes that share ontologies with SCRIB: view

No data available for SIGNOR curated interactions for SCRIB Gene

Drugs & Compounds for SCRIB Gene

No Compound Related Data Available

Transcripts for SCRIB Gene

mRNA/cDNA for SCRIB Gene

CRISPR Products

Clone Products

  • Addgene plasmids for SCRIB

Alternative Splicing Database (ASD) splice patterns (SP) for SCRIB Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b · 11c ^ 12a · 12b · 12c ^ 13a · 13b · 13c ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11: - - - - -
SP12:
SP13:
SP14:
SP15:
SP16: -
SP17: - -

ExUns: 20 ^ 21 ^ 22 ^ 23 ^ 24a · 24b ^ 25 ^ 26a · 26b ^ 27a · 27b ^ 28 ^ 29 ^ 30 ^ 31 ^ 32 ^ 33a · 33b ^ 34 ^ 35a · 35b · 35c · 35d ^ 36 ^ 37 ^ 38 ^
SP1: -
SP2: -
SP3: -
SP4:
SP5:
SP6:
SP7: -
SP8:
SP9:
SP10:
SP11: - -
SP12:
SP13:
SP14: - -
SP15:
SP16:
SP17:

ExUns: 39 ^ 40a · 40b · 40c
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:
SP15:
SP16:
SP17:

Relevant External Links for SCRIB Gene

GeneLoc Exon Structure for
SCRIB
ECgene alternative splicing isoforms for
SCRIB

Expression for SCRIB Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for SCRIB Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for SCRIB Gene

This gene is overexpressed in Pancreas (12.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for SCRIB Gene



Protein tissue co-expression partners for SCRIB Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of SCRIB Gene:

SCRIB

SOURCE GeneReport for Unigene cluster for SCRIB Gene:

Hs.436329

mRNA Expression by UniProt/SwissProt for SCRIB Gene:

Q14160-SCRIB_HUMAN
Tissue specificity: Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).

Evidence on tissue expression from TISSUES for SCRIB Gene

  • Nervous system(4.5)
  • Liver(4.2)
  • Bone marrow(4.1)
  • Skin(2.4)
genes like me logo Genes that share expression patterns with SCRIB: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for SCRIB Gene

Orthologs for SCRIB Gene

This gene was present in the common ancestor of animals.

Orthologs for SCRIB Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia SCRIB 33 32
  • 99.01 (n)
OneToOne
dog
(Canis familiaris)
Mammalia SCRIB 33 32
  • 84.39 (n)
OneToOne
cow
(Bos Taurus)
Mammalia SCRIB 33 32
  • 83.9 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Scrib 17 33 32
  • 81.85 (n)
rat
(Rattus norvegicus)
Mammalia Scrib 32
  • 81.57 (n)
oppossum
(Monodelphis domestica)
Mammalia SCRIB 33
  • 72 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia SCRIB 33
  • 63 (a)
OneToOne
chicken
(Gallus gallus)
Aves SCRIB 33
  • 69 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia scrib 32
  • 70.7 (n)
zebrafish
(Danio rerio)
Actinopterygii scrib 33 32
  • 65.31 (n)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta scrib 33
  • 27 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea let-413 33 34 32
  • 46.86 (n)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.7054 33
  • 42 (a)
OneToMany
Species where no ortholog for SCRIB was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for SCRIB Gene

ENSEMBL:
Gene Tree for SCRIB (if available)
TreeFam:
Gene Tree for SCRIB (if available)
Aminode:
Evolutionary constrained regions (ECRs) for SCRIB: view image

Paralogs for SCRIB Gene

(20) SIMAP similar genes for SCRIB Gene using alignment to 4 proteins:

  • SCRIB_HUMAN
  • A0PJK8_HUMAN
  • H0YCG0_HUMAN
  • H0YDF9_HUMAN
genes like me logo Genes that share paralogs with SCRIB: view

Variants for SCRIB Gene

Sequence variations from dbSNP and Humsavar for SCRIB Gene

SNP ID Clin Chr 08 pos Variation AA Info Type
rs1057518681 likely-pathogenic, Verheij syndrome, not provided 143,816,821(-) T/C upstream_transcript_variant
rs1302482009 Neural tube defects (NTD) [MIM:182940] 143,810,730(-) G/A coding_sequence_variant, missense_variant
rs1554642022 pathogenic, Verheij syndrome 143,816,981(-) G/A upstream_transcript_variant
rs1554642573 pathogenic, Verheij syndrome 143,817,402(-) AG/GGGGCTGGGCCAGGGTCAA upstream_transcript_variant
rs1563818514 pathogenic, Verheij syndrome 143,816,752(-) A/G upstream_transcript_variant

Structural Variations from Database of Genomic Variants (DGV) for SCRIB Gene

Variant ID Type Subtype PubMed ID
esv2738038 CNV deletion 23290073
nsv1140948 CNV deletion 24896259
nsv1161815 CNV deletion 26073780
nsv398483 CNV insertion 16902084
nsv466009 CNV loss 19166990
nsv612895 CNV loss 21841781
nsv612896 CNV loss 21841781
nsv612897 CNV loss 21841781
nsv6442 CNV deletion 18451855
nsv950014 CNV deletion 24416366

Variation tolerance for SCRIB Gene

Residual Variation Intolerance Score: 22.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.87; 79.42% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for SCRIB Gene

Human Gene Mutation Database (HGMD)
SCRIB
SNPedia medical, phenotypic, and genealogical associations of SNPs for
SCRIB

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for SCRIB Gene

Disorders for SCRIB Gene

MalaCards: The human disease database

(6) MalaCards diseases for SCRIB Gene - From: HGMD, DISEASES, and GeneCards

Disorder Aliases PubMed IDs
neural tube defects
  • neural tube defects, susceptibility to
tick-borne encephalitis
  • central european encephalitis
verheij syndrome
  • vrjs
craniorachischisis
anencephaly
  • anph
- elite association - COSMIC cancer census association via MalaCards
Search SCRIB in MalaCards View complete list of genes associated with diseases

UniProtKB/Swiss-Prot

SCRIB_HUMAN
  • Neural tube defects (NTD) [MIM:182940]: Congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele and spina bifida, which result from the failure of fusion in the cranial and spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic and environmental components. {ECO:0000269 PubMed:22095531}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Additional Disease Information for SCRIB

genes like me logo Genes that share disorders with SCRIB: view

No data available for Genatlas for SCRIB Gene

Publications for SCRIB Gene

  1. Thyrotropin receptor trafficking relies on the hScrib-betaPIX-GIT1-ARF6 pathway. (PMID: 15775968) Lahuna O … Misrahi M (The EMBO journal 2005) 3 4 23 56
  2. The tumor suppressor Scrib selectively interacts with specific members of the zyxin family of proteins. (PMID: 16137684) Petit MM … Van de Ven WJ (FEBS letters 2005) 3 4 23 56
  3. The tumor suppressor Scrib interacts with the zyxin-related protein LPP, which shuttles between cell adhesion sites and the nucleus. (PMID: 15649318) Petit MM … Van de Ven WJ (BMC cell biology 2005) 3 4 23 56
  4. hScrib is a functional homologue of the Drosophila tumour suppressor Scribble. (PMID: 14681682) Dow LE … Humbert PO (Oncogene 2003) 2 3 23 56
  5. Human scribble (Vartul) is targeted for ubiquitin-mediated degradation by the high-risk papillomavirus E6 proteins and the E6AP ubiquitin-protein ligase. (PMID: 11027293) Nakagawa S … Huibregtse JM (Molecular and cellular biology 2000) 2 3 4 56

Products for SCRIB Gene

Sources for SCRIB Gene