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Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C term... See more...
Aliases for RPS27A Gene
Aliases for RPS27A Gene
External Ids for RPS27A Gene
- HGNC: 10417
- Entrez Gene: 6233
- Ensembl: ENSG00000143947
- OMIM: 191343
- UniProtKB: P62979
Previous GeneCards Identifiers for RPS27A Gene
- GC02P055497
- GC02P055646
- GC02P055417
- GC02P055434
- GC02P055371
- GC02P055313
- GC02P055459
- GC02P055193
Summaries for RPS27A Gene
-
Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq, Sep 2008]
GeneCards Summary for RPS27A Gene
RPS27A (Ribosomal Protein S27a) is a Protein Coding gene. Diseases associated with RPS27A include Machado-Joseph Disease and Neurodegeneration With Brain Iron Accumulation 2A. Among its related pathways are Regulation of TP53 Activity and Ion channel transport. Gene Ontology (GO) annotations related to this gene include structural constituent of ribosome. An important paralog of this gene is ENSG00000231767.
UniProtKB/Swiss-Prot Summary for RPS27A Gene
-
[Ubiquitin]: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
-
[40S ribosomal protein S27a]: Component of the 40S subunit of the ribosome.
Additional gene information for RPS27A Gene
No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RPS27A Gene
Genomics for RPS27A Gene
GeneHancer (GH) Regulatory Elements for RPS27A Gene
Promoters and enhancers for RPS27A Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication |
Request up-to-date GeneHancer data (full dataset)
GeneHancers around RPS27A on UCSC Golden Path with GeneCards custom track
Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RPS27A
- Top Transcription factor binding sites by QIAGEN in the RPS27A gene promoter:
-
- AP-1
- c-Fos
- c-Jun
- Hlf
- IRF-1
Genomic Locations for RPS27A Gene
Genomic Locations for RPS27A Gene
- chr2:55,231,903-55,235,853
- (GRCh38/hg38)
- Size:
- 3,951 bases
- Orientation:
- Plus strand
- chr2:55,459,039-55,462,989
- (GRCh37/hg19)
- Size:
- 3,951 bases
- Orientation:
- Plus strand
Genomic View for RPS27A Gene
Genes around RPS27A on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 2p16.1 by HGNC
- 2p16.1 by Entrez Gene
- 2p16.1 by Ensembl
RPS27A Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for RPS27A Gene
Proteins for RPS27A Gene
-
Protein details for RPS27A Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- P62979-RS27A_HUMAN
- Recommended name:
- Ubiquitin-40S ribosomal protein S27a
- Protein Accession:
- P62979
- P02248
- P02249
- P02250
- P14798
- P62988
- Q29120
- Q6LBL4
- Q6LDU5
- Q8WYN8
- Q91887
- Q91888
- Q9BQ77
- Q9BWD6
- Q9BX98
- Q9UEF2
- Q9UEG1
- Q9UEK8
- Q9UPK7
Protein attributes for RPS27A Gene
- Size:
- 156 amino acids
- Molecular mass:
- 17965 Da
- Quaternary structure:
-
- Ribosomal protein S27a is part of the 40S ribosomal subunit.
- Miscellaneous:
-
- Ubiquitin is encoded by 4 different genes. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains.
- For a better understanding, features related to ubiquitin are only indicated for the first chain.
Protein Expression for RPS27A Gene
Post-translational modifications for RPS27A Gene
- [Ubiquitin]: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291). Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30 (PubMed:25527291).
- [Ubiquitin]: Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones.
-
Ubiquitination at Lys6, Lys11, Lys27, Lys29, Lys48, Lys63, Lys113, Lys143, and Lys152
- NX_P62979 (NX_P62979-1)
- Modification sites at PhosphoSitePlus
Other Protein References for RPS27A Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibody Products
- G Biosciences Immunotag™ RPS27A Antibody
- Creative Diagnostics Anti-RPS27A (full length) polyclonal antibody
- Novus Biologicals Antibodies for RPS27A
-
Abcam antibodies for RPS27A
- Invitrogen Antibodies for RPS27A (AFLGC-RPS27A)
- antibodies-online: Show 49 available RPS27A Antibodies ranked by validation data
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Recommended
- 28 WB Antibodies (anti-human)
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- 3 IHC (p) Antibodies (anti-human)
- 1 IF/ICC Antibody (anti-human)
- 1 IHC Antibody (anti-human)
- 20 WB Antibodies (anti-mouse)
- 12 IF (p) Antibodies (anti-mouse)
- 3 IHC (p) Antibodies (anti-mouse)
- 1 IHC Antibody (anti-mouse)
- 17 WB Antibodies (anti-rat)
- 12 IF (p) Antibodies (anti-rat)
- 3 IHC (p) Antibodies (anti-rat)
- 1 IF/ICC Antibody (anti-rat)
Protein Products
-
OriGene Purified Proteins for RPS27A
- Search Origene for MassSpec and Protein Over-expression Lysates for RPS27A
- Origene Custom Protein Services for RPS27A
- ProSpec Recombinant Proteins for RPS27A
-
Signalway Proteins for RPS27A
No data available for DME Specific Peptides for RPS27A Gene
Domains & Families for RPS27A Gene
Gene Families for RPS27A Gene
- HGNC:
- Human Protein Atlas (HPA):
-
- Predicted intracellular proteins
- Ribosomal proteins
Protein Domains for RPS27A Gene
- Blocks:
-
- Ribosomal protein S27a
- InterPro:
- ProtoNet:
Suggested Antigen Peptide Sequences for RPS27A Gene
- GenScript: Design optimal peptide antigens:
-
- cDNA, FLJ96793, Homo sapiens ribosomal protein S27a (RPS27A), mRNA (B2RDW1_HUMAN)
- RPS27A protein (Q49A90_HUMAN)
- Ribosomal protein S27a (Q5RKT7_HUMAN)
- Ribosomal protein S27A (Q8WYN9_HUMAN)
- Ubiquitin carboxyl extension protein 80 (RS27A_HUMAN)
Graphical View of Domain Structure for InterPro Entry
P62979- Family:
-
- In the N-terminal section; belongs to the ubiquitin family.
- In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.
Function for RPS27A Gene
Molecular function for RPS27A Gene
- UniProtKB/Swiss-Prot Function:
- [Ubiquitin]: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
- UniProtKB/Swiss-Prot Function:
- [40S ribosomal protein S27a]: Component of the 40S subunit of the ribosome.
- GENATLAS Biochemistry:
- 60S ribosomal protein S27A
Phenotypes From GWAS Catalog for RPS27A Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0003723 | RNA binding | HDA | 22658674 |
| GO:0003735 | structural constituent of ribosome | HDA | 15883184 |
| GO:0005515 | protein binding | IPI,IEA | 15936278 |
| GO:0031386 | protein tag | IBA | 21873635 |
| GO:0031625 | ubiquitin protein ligase binding | IBA | 21873635 |
Phenotypes for RPS27A Gene
- GenomeRNAi human phenotypes for RPS27A:
-
- G0/1 arrest
- Increased G2M DNA content, increased 8N DNA content
- Decreased Hepatitis C virus replication
- Decreased shRNA abundance (Z-score < -2)
- Increased shRNA abundance (Z-score > 2)
- Negative genetic interaction between BLM-/- and BLM+/+
- Decreased viability of wild-type and TP53 knockout cells, decreased TP53 protein expression ratio (wild-type / TP53 knockout cells)
- Decreased viability
- no effect
- Decreased cell number
- Increased vaccinia virus (VACV) infection
- Nucleoplasmic pre-40S maturation defects
- Nuclear 40S maturation defects
- Decreased NF-kappaB reporter expression
- Decreased influenza A H1N1 (A/WSN/33) virus numbers
- Decreased influenza A/WSN/33 replication
- Increased Sindbis virus (SINV) infection
- shRNA abundance <= 50%
- Decreased IL-8 secretion
- Increased viability
Animal Model Products
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
CRISPR Products
- Applied Biological Materials (abm): CRISPR Clones for RPS27A - Select products 50% OFF >
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- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for RPS27A
- genomics-online: gRNA clones - Search for available RPS27A gRNA products
- Overview of 160 available RPS27A gene related products
-
Santa Cruz Biotechnology (SCBT) CRISPR for RPS27A
miRNA for RPS27A Gene
- miRTarBase miRNAs that target RPS27A
-
- hsa-mir-30b-5p (MIRT046153)
- hsa-mir-23b-3p (MIRT046242)
- hsa-mir-30c-5p (MIRT047980)
- hsa-mir-26a-5p (MIRT050094)
- hsa-mir-17-5p (MIRT050798)
- hsa-mir-578 (MIRT448338)
- hsa-mir-643 (MIRT448339)
- hsa-mir-510-3p (MIRT464836)
- hsa-mir-653-5p (MIRT464837)
- hsa-mir-106a-3p (MIRT464838)
- hsa-mir-488-3p (MIRT464839)
- hsa-mir-6730-5p (MIRT464840)
- hsa-mir-5681a (MIRT464841)
- hsa-mir-519c-3p (MIRT464842)
- hsa-mir-519b-3p (MIRT464843)
- hsa-mir-519a-3p (MIRT464844)
- hsa-mir-651-3p (MIRT464845)
- hsa-mir-526b-3p (MIRT464846)
- hsa-mir-20a-5p (MIRT464847)
- hsa-mir-519d-3p (MIRT464848)
- hsa-mir-20b-5p (MIRT464849)
- hsa-mir-93-5p (MIRT464850)
- hsa-mir-106a-5p (MIRT464851)
- hsa-mir-106b-5p (MIRT464852)
- hsa-mir-454-3p (MIRT464853)
- hsa-mir-4295 (MIRT464854)
- hsa-mir-301a-3p (MIRT464855)
- hsa-mir-3666 (MIRT464856)
- hsa-mir-301b-3p (MIRT464857)
- hsa-mir-130b-3p (MIRT464858)
- hsa-mir-130a-3p (MIRT464859)
- hsa-mir-190a-3p (MIRT505257)
- hsa-mir-5011-5p (MIRT505258)
- hsa-mir-1277-5p (MIRT505259)
- hsa-mir-450a-1-3p (MIRT533380)
- hsa-mir-6716-5p (MIRT533381)
- hsa-mir-6885-3p (MIRT553209)
- hsa-mir-7160-5p (MIRT697903)
- hsa-mir-4722-5p (MIRT697904)
- hsa-mir-4649-3p (MIRT697905)
- hsa-mir-4768-3p (MIRT697906)
- hsa-mir-665 (MIRT697907)
- hsa-mir-1273f (MIRT783317)
- hsa-mir-2681-5p (MIRT784347)
- hsa-mir-4756-3p (MIRT786631)
- hsa-mir-4797-5p (MIRT786840)
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for RPS27A
- Browse OriGene Inhibitory RNA Products For RPS27A
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RPS27A
- genomics-online: shRNA clones - Search for available RPS27A shRNA products
- Overview of 160 available RPS27A gene related products
Clone Products
- Applied Biological Materials (abm): Clones for RPS27A - Select products 50% OFF >
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-
- MG201163
- MR201163
- MR201163L3
- MR201163L4
- MR228180
- RC209696
- RC209696L1
- RC209696L2
- RC209696L3
- RC209696L4
- RC227076
- RC227076L1
- RC227076L2
- RC227076L3
- RC227076L4
- RC229601
- RC229601L3
- RC229601L4
- RG209696
- RG227076
- RG229601
- RR203186
- RR203186L3
- RR203186L4
- MR201163L3V
- MR201163L4V
- RC209696L1V
- RC209696L2V
- RC209696L3V
- RC209696L4V
- RC227076L1V
- RC227076L2V
- RC227076L3V
- RC227076L4V
- RC229601L3V
- RC229601L4V
- RR203186L3V
- RR203186L4V
- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Sino Biological Human cDNA Clone for RPS27A
- Addgene plasmids for RPS27A
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for RPS27A
No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RPS27A Gene
Localization for RPS27A Gene
Subcellular locations from UniProtKB/Swiss-Prot for RPS27A Gene
- [Ubiquitin]: Cytoplasm. Nucleus.
| Compartment | Confidence |
|---|---|
| cytosol | 5 |
| plasma membrane | 4 |
| mitochondrion | 4 |
| nucleus | 4 |
| endoplasmic reticulum | 4 |
| endosome | 4 |
| extracellular | 3 |
| cytoskeleton | 2 |
| peroxisome | 1 |
| lysosome | 1 |
| golgi apparatus | 1 |
- Cytosol (3)
- Endoplasmic reticulum (3)
- Nucleoli (3)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0005615 | extracellular space | HDA | 16502470 |
| GO:0005634 | nucleus | IBA | 21873635 |
| GO:0005654 | nucleoplasm | TAS | -- |
| GO:0005730 | nucleolus | HDA | 16791210 |
| GO:0005737 | cytoplasm | IBA | 21873635 |
Pathways & Interactions for RPS27A Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | CDK-mediated phosphorylation and removal of Cdc6 |
.93
.93
|
.80
.78
.70
.47
.31
|
| 2 | RET signaling |
.92
|
.92
|
| 3 | Activated TLR4 signalling | ||
| 4 | Viral mRNA Translation |
CFTR translational fidelity (class I mutations)
.79
.77
.55
|
|
| 5 | HIV Life Cycle |
.91
.64
|
.45
|
Pathways by source for RPS27A Gene
2 BioSystems pathways for RPS27A Gene
355 Reactome pathways for RPS27A Gene
1 KEGG pathway for RPS27A Gene
1 GeneGo (Thomson Reuters) pathway for RPS27A Gene
- CFTR translational fidelity (class I mutations)
1 Cell Signaling Technology pathway for RPS27A Gene
Interacting Proteins for RPS27A Gene
Selected Interacting proteins:
P62979-RS27A_HUMAN
ENSP00000272317
for RPS27A Gene via
UniProtKB
MINT
STRING
IID
GPS-Prot Interaction Network for RPS27A
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000122 | negative regulation of transcription by RNA polymerase II | TAS | -- |
| GO:0000184 | nuclear-transcribed mRNA catabolic process, nonsense-mediated decay | TAS | -- |
| GO:0000187 | activation of MAPK activity | TAS | -- |
| GO:0000209 | protein polyubiquitination | TAS | -- |
| GO:0000715 | nucleotide-excision repair, DNA damage recognition | TAS | -- |