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Aliases for RPL35A Gene

Aliases for RPL35A Gene

  • Ribosomal Protein L35a 2 3 5
  • Cell Growth-Inhibiting Gene 33 Protein 3 4
  • Large Ribosomal Subunit Protein EL33 3 4
  • 60S Ribosomal Protein L35a 3
  • DBA5 3
  • L35A 3
  • EL33 3

External Ids for RPL35A Gene

Previous GeneCards Identifiers for RPL35A Gene

  • GC03U990068
  • GC03P199076
  • GC03P199003
  • GC03P199165
  • GC03P197676
  • GC03P194979

Summaries for RPL35A Gene

Entrez Gene Summary for RPL35A Gene

  • Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Alternative splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Oct 2015]

GeneCards Summary for RPL35A Gene

RPL35A (Ribosomal Protein L35a) is a Protein Coding gene. Diseases associated with RPL35A include Diamond-Blackfan Anemia 5 and Diamond-Blackfan Anemia. Among its related pathways are Viral mRNA Translation and Influenza Viral RNA Transcription and Replication. Gene Ontology (GO) annotations related to this gene include tRNA binding.

UniProtKB/Swiss-Prot for RPL35A Gene

  • Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site.

Gene Wiki entry for RPL35A Gene

Additional gene information for RPL35A Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RPL35A Gene

Genomics for RPL35A Gene

GeneHancer (GH) Regulatory Elements for RPL35A Gene

Promoters and enhancers for RPL35A Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH03J197948 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 662.7 +0.9 892 3.9 HDGF PKNOX1 FOXA2 SMAD1 ARNT ARID4B NEUROD1 SIN3A DMAP1 ZNF2 RPL35A IQCG ENSG00000234742 LOC100131868 LOC220729
GH03J197956 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 27.9 +9.2 9227 6.1 HDGF HNRNPUL1 PKNOX1 CLOCK SMAD1 MLX ARNT ARID4B SIN3A FEZF1 IQCG LMLN ENSG00000234742 LOC220729 LRCH3 RPL35A LOC100131868
GH03J196968 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 11.7 -980.6 -980588 2.4 PKNOX1 SIN3A ZNF48 YY1 ZNF766 GLIS2 ZNF207 ZNF143 ATF7 RUNX3 PIGZ SDHAP1 UBXN7 ENSG00000234742 LOC220729 LRCH3 SENP5 RPL35A NCBP2-AS1 UBXN7-AS1
GH03J197539 Enhancer 1.3 Ensembl ENCODE dbSUPER 13.7 -409.0 -408982 3.4 PKNOX1 CLOCK NFIB NEUROD1 SIN3A ZBTB7B ETS1 EGR1 SCRT2 ZFP91 ENSG00000234742 RPL35A LOC220729 ENSG00000230732 BDH1 GC03P197515
GH03J197392 Enhancer 1.3 FANTOM5 Ensembl ENCODE 10.7 -555.6 -555621 3.7 HDGF PKNOX1 ATF1 IRF4 FOS ATF7 RUNX3 RXRA JUNB ZNF592 UBXN7 ENSG00000234742 LRCH3 UBXN7-AS1 LOC220729 RPL35A DLG1 GC03M197355 LOC101926923
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RPL35A on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the RPL35A gene promoter:
  • MEF-2A
  • aMEF-2
  • AREB6
  • Arnt
  • AhR
  • NRSF form 1
  • NRSF form 2
  • Zic1
  • AML1a
  • POU3F1

Genomic Locations for RPL35A Gene

Genomic Locations for RPL35A Gene
6,631 bases
Plus strand
6,624 bases
Plus strand

Genomic View for RPL35A Gene

Genes around RPL35A on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RPL35A Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RPL35A Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RPL35A Gene

Proteins for RPL35A Gene

  • Protein details for RPL35A Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    60S ribosomal protein L35a
    Protein Accession:
    Secondary Accessions:
    • Q08ES9
    • Q9BVN7

    Protein attributes for RPL35A Gene

    110 amino acids
    Molecular mass:
    12538 Da
    Quaternary structure:
    No Data Available
    • Knockdown of RPL35A in hematopoietic cell lines results in decreased cell proliferation, increased apoptosis, decreased biogenesis of mature 60S ribosomal subunit, and abnormal processing of large ribosomal subunit rRNA.

    Three dimensional structures from OCA and Proteopedia for RPL35A Gene

neXtProt entry for RPL35A Gene

Post-translational modifications for RPL35A Gene

  • Ubiquitination at posLast=9595, Lys45, and posLast=88
  • Modification sites at PhosphoSitePlus

Other Protein References for RPL35A Gene

No data available for DME Specific Peptides for RPL35A Gene

Domains & Families for RPL35A Gene

Gene Families for RPL35A Gene

Human Protein Atlas (HPA):
  • Disease related genes
  • Predicted intracellular proteins
  • Ribosomal proteins

Protein Domains for RPL35A Gene

Suggested Antigen Peptide Sequences for RPL35A Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the eukaryotic ribosomal protein eL33 family.
  • Belongs to the eukaryotic ribosomal protein eL33 family.
genes like me logo Genes that share domains with RPL35A: view

Function for RPL35A Gene

Molecular function for RPL35A Gene

UniProtKB/Swiss-Prot Function:
Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site.
GENATLAS Biochemistry:
60S ribosomal protein L35A,12kDa

Phenotypes From GWAS Catalog for RPL35A Gene

Gene Ontology (GO) - Molecular Function for RPL35A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000049 tRNA binding IEA --
GO:0003723 RNA binding IEA,HDA 22658674
GO:0003735 structural constituent of ribosome NAS,IEA 12962325
GO:0005515 protein binding IPI 24965446
genes like me logo Genes that share ontologies with RPL35A: view
genes like me logo Genes that share phenotypes with RPL35A: view

Human Phenotype Ontology for RPL35A Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RPL35A

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targets and HOMER Transcription for RPL35A Gene

Localization for RPL35A Gene

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RPL35A gene
Compartment Confidence
extracellular 5
cytosol 5
mitochondrion 1

Gene Ontology (GO) - Cellular Components for RPL35A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005622 intracellular IEA --
GO:0005739 mitochondrion IEA --
GO:0005829 cytosol TAS --
GO:0005840 ribosome IEA --
GO:0016020 membrane HDA 19946888
genes like me logo Genes that share ontologies with RPL35A: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot and Subcellular locations from the Human Protein Atlas (HPA) for RPL35A Gene

Pathways & Interactions for RPL35A Gene

genes like me logo Genes that share pathways with RPL35A: view

Pathways by source for RPL35A Gene

1 KEGG pathway for RPL35A Gene
1 GeneGo (Thomson Reuters) pathway for RPL35A Gene

Gene Ontology (GO) - Biological Process for RPL35A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay TAS --
GO:0002181 cytoplasmic translation IBA --
GO:0006364 rRNA processing TAS --
GO:0006412 translation IEA,NAS 12962325
GO:0006413 translational initiation TAS --
genes like me logo Genes that share ontologies with RPL35A: view

No data available for SIGNOR curated interactions for RPL35A Gene

Drugs & Compounds for RPL35A Gene

No Compound Related Data Available

Transcripts for RPL35A Gene

mRNA/cDNA for RPL35A Gene

Unigene Clusters for RPL35A Gene

Ribosomal protein L35a:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RPL35A

Alternative Splicing Database (ASD) splice patterns (SP) for RPL35A Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5a · 5b
SP1: -
SP3: -

Relevant External Links for RPL35A Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for RPL35A Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for RPL35A Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for RPL35A Gene

This gene is overexpressed in Nasal epithelium (14.9), Pancreas (7.2), and Lung (6.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for RPL35A Gene

Protein tissue co-expression partners for RPL35A Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of RPL35A Gene:


SOURCE GeneReport for Unigene cluster for RPL35A Gene:


Evidence on tissue expression from TISSUES for RPL35A Gene

  • Intestine(4.6)
  • Bone marrow(4.5)
  • Nervous system(4.2)
  • Lung(3.9)
  • Muscle(3.8)
  • Pancreas(3.6)
  • Liver(3.3)
  • Skin(2.9)
  • Lymph node(2.8)
  • Adrenal gland(2.7)
  • Gall bladder(2.6)
  • Heart(2.6)
  • Kidney(2.6)
  • Spleen(2.6)
  • Stomach(2.6)
  • Thyroid gland(2.6)
  • Blood(2.4)
  • Eye(2.3)
  • Bone(2.1)

Phenotype-based relationships between genes and organs from Gene ORGANizer for RPL35A Gene

Germ Layers:
  • ectoderm
  • endoderm
  • mesoderm
  • cardiovascular
  • digestive
  • endocrine
  • immune
  • integumentary
  • lymphatic
  • nervous
  • reproductive
  • respiratory
  • skeletal muscle
  • skeleton
  • urinary
Head and neck:
  • brain
  • cheek
  • chin
  • cranial nerve
  • ear
  • eye
  • eyelid
  • face
  • head
  • inner ear
  • jaw
  • lip
  • mandible
  • maxilla
  • middle ear
  • mouth
  • neck
  • nose
  • outer ear
  • skull
  • aorta
  • chest wall
  • clavicle
  • heart
  • heart valve
  • lung
  • rib
  • rib cage
  • scapula
  • sternum
  • intestine
  • kidney
  • large intestine
  • pelvis
  • penis
  • ureter
  • urethra
  • urinary bladder
  • ankle
  • arm
  • digit
  • elbow
  • femur
  • fibula
  • finger
  • foot
  • forearm
  • hand
  • hip
  • humerus
  • knee
  • lower limb
  • radius
  • shin
  • shoulder
  • thigh
  • tibia
  • toe
  • ulna
  • upper limb
  • wrist
  • blood
  • blood vessel
  • bone marrow
  • coagulation system
  • peripheral nervous system
  • red blood cell
  • skin
  • spinal column
  • vertebrae
  • white blood cell
genes like me logo Genes that share expression patterns with RPL35A: view

No data available for mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for RPL35A Gene

Orthologs for RPL35A Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for RPL35A Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia RPL35A 34 34 33
  • 99.7 (n)
(Ornithorhynchus anatinus)
Mammalia RPL35A 34
  • 99 (a)
(Monodelphis domestica)
Mammalia RPL35A 34
  • 96 (a)
(Bos Taurus)
Mammalia -- 34
  • 95 (a)
RPL35A 34 33
  • 91.21 (n)
(Rattus norvegicus)
Mammalia LOC100359498 33
  • 92.12 (n)
(Canis familiaris)
Mammalia RPL35A 34 33
  • 91.52 (n)
(Mus musculus)
Mammalia Rpl35a 16 34 33
  • 90.91 (n)
(Gallus gallus)
Aves RPL35A 34 33
  • 81.82 (n)
(Anolis carolinensis)
Reptilia RPL35A 34
  • 94 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia rpl35a 33
  • 81.82 (n)
(Danio rerio)
Actinopterygii rpl35a 34 33
  • 75.45 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP002754 33
  • 62.35 (n)
fruit fly
(Drosophila melanogaster)
Insecta RpL35A 34 33
  • 61.73 (n)
CG2099 35
  • 61 (a)
(Caenorhabditis elegans)
Secernentea rpl-33 34 35 33
  • 59.94 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RPL33B 34 33
  • 53.53 (n)
RPL33A 34
  • 51 (a)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0D07405g 33
  • 52.24 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ACR120C 33
  • 51.92 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT1G74270 33
  • 57 (n)
(Oryza sativa)
Liliopsida Os02g0785800 33
  • 55.88 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 77 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU09109 33
  • 52.29 (n)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes rpl35b 33
  • 52.29 (n)
Species where no ortholog for RPL35A was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RPL35A Gene

Gene Tree for RPL35A (if available)
Gene Tree for RPL35A (if available)
Evolutionary constrained regions (ECRs) for RPL35A: view image

Paralogs for RPL35A Gene

genes like me logo Genes that share paralogs with RPL35A: view

No data available for Paralogs for RPL35A Gene

Variants for RPL35A Gene

Sequence variations from dbSNP and Humsavar for RPL35A Gene

SNP ID Clin Chr 03 pos Variation AA Info Type
rs10022 likely-benign, Diamond-Blackfan anemia 197,955,787(+) C/A/T 3_prime_UTR_variant
rs116840807 pathogenic, Diamond-Blackfan anemia 5 197,951,225(+) TCTTCTT/TCTT coding_sequence_variant, inframe_deletion
rs116840808 pathogenic, Diamond-Blackfan anemia 5, Diamond-Blackfan anemia 5 (DBA5) [MIM:612528] 197,951,244(+) G/A coding_sequence_variant, missense_variant
rs116840809 pathogenic, Diamond-Blackfan anemia 5 197,954,142(+) C/T coding_sequence_variant, stop_gained
rs116874993 likely-benign, Diamond-Blackfan anemia, not specified 197,955,779(+) C/T 3_prime_UTR_variant

Structural Variations from Database of Genomic Variants (DGV) for RPL35A Gene

Variant ID Type Subtype PubMed ID
dgv5044n100 CNV gain 25217958
dgv5045n100 CNV gain 25217958
dgv8865n54 CNV gain 21841781
esv2751989 CNV gain 17911159
esv2763718 CNV gain+loss 21179565
esv3575648 CNV gain 25503493
esv3599324 CNV gain 21293372
esv3599329 CNV gain 21293372
esv3599331 CNV gain 21293372
esv3893855 CNV gain 25118596
nsv1009299 CNV gain 25217958
nsv1013844 CNV gain 25217958
nsv527527 CNV gain 19592680
nsv979915 CNV duplication 23825009
nsv999711 CNV gain 25217958

Variation tolerance for RPL35A Gene

Residual Variation Intolerance Score: 55.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.60; 12.84% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RPL35A Gene

Human Gene Mutation Database (HGMD)
SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RPL35A Gene

Disorders for RPL35A Gene

MalaCards: The human disease database

(4) MalaCards diseases for RPL35A Gene - From: HGMD, OMIM, ClinVar, GTR, Orphanet, Swiss-Prot, DISEASES, and GeneCards

Disorder Aliases PubMed IDs
diamond-blackfan anemia 5
  • dba5
diamond-blackfan anemia
  • diamond-blackfan anemia 1
macrocytic anemia
  • anemia macrocytic
pierre robin syndrome
  • prbns
- elite association - COSMIC cancer census association via MalaCards


  • Diamond-Blackfan anemia 5 (DBA5) [MIM:612528]: A form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. {ECO:0000269 PubMed:18535205}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Additional Disease Information for RPL35A

Genetic Association Database
Human Genome Epidemiology Navigator
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with RPL35A: view

No data available for Genatlas for RPL35A Gene

Publications for RPL35A Gene

  1. The human L35a ribosomal protein (RPL35A) gene is located at chromosome band 3q29-qter. (PMID: 8786106) Colombo P … Fried M (Genomics 1996) 2 3 4 22 58
  2. cDNA encoding the human homologue of rat ribosomal protein L35a. (PMID: 2388839) Herzog H … Schweiger M (Nucleic acids research 1990) 3 4 22 58
  3. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PMID: 20877624) Hendrickson SL … O'Brien SJ (PloS one 2010) 3 44 58
  4. Mutations in the ribosomal protein genes in Japanese patients with Diamond-Blackfan anemia. (PMID: 20378560) Konno Y … Ito E (Haematologica 2010) 3 44 58
  5. Diamond-Blackfan anemia: genotype-phenotype correlations in Italian patients with RPL5 and RPL11 mutations. (PMID: 19773262) Quarello P … Ramenghi U (Haematologica 2010) 3 44 58

Products for RPL35A Gene

Sources for RPL35A Gene

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