The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. [provided by RefSeq, Jul 2008] See more...

Aliases for RNF5 Gene

Aliases for RNF5 Gene

  • Ring Finger Protein 5 2 3 5
  • RMA1 2 3 4
  • Ring Finger Protein 5, E3 Ubiquitin Protein Ligase 2 3
  • RING-Type E3 Ubiquitin Transferase RNF5 3 4
  • E3 Ubiquitin-Protein Ligase RNF5 3 4
  • Ram1 Homolog 3 4
  • Protein G16 3 4
  • RING5 2 3
  • NG2 2 4
  • G16 2 4
  • RING Finger Protein 5 4
  • EC 2.3.2.27 4
  • HsRma1 4
  • RNF5 5

External Ids for RNF5 Gene

Previous GeneCards Identifiers for RNF5 Gene

  • GC06P032173
  • GC06P031916
  • GC06P032217
  • GC06P032254
  • GC06P032146
  • GC06P032402
  • GC06P032530
  • GC06P032660
  • GC06P033439
  • GC06P032180
  • GC06P032368
  • GC06P047337
  • GC06P049380

Summaries for RNF5 Gene

Entrez Gene Summary for RNF5 Gene

  • The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. [provided by RefSeq, Jul 2008]

GeneCards Summary for RNF5 Gene

RNF5 (Ring Finger Protein 5) is a Protein Coding gene. Diseases associated with RNF5 include Cystic Fibrosis. Among its related pathways are Calnexin/calreticulin cycle and CDK-mediated phosphorylation and removal of Cdc6. Gene Ontology (GO) annotations related to this gene include ligase activity and ubiquitin-protein transferase activity. An important paralog of this gene is RNF185.

UniProtKB/Swiss-Prot Summary for RNF5 Gene

  • Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of STING1 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses.

Gene Wiki entry for RNF5 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RNF5 Gene

Genomics for RNF5 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for RNF5 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RNF5 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RNF5

Top Transcription factor binding sites by QIAGEN in the RNF5 gene promoter:
  • ARP-1
  • E2F
  • E2F-1
  • E2F-2
  • E2F-3a
  • E2F-4
  • E2F-5
  • E47
  • p53
  • Tal-1beta

Genomic Locations for RNF5 Gene

Latest Assembly
chr6:32,178,405-32,180,793
(GRCh38/hg38)
Size:
2,389 bases
Orientation:
Plus strand

Previous Assembly
chr6:32,146,182-32,148,570
(GRCh37/hg19 by Entrez Gene)
Size:
2,389 bases
Orientation:
Plus strand

chr6:32,146,131-32,151,930
(GRCh37/hg19 by Ensembl)
Size:
5,800 bases
Orientation:
Plus strand

Alternative Locations (GRCh38/hg38)

  • chr6(ALT_REF_LOCI_1):3,511,007-3,513,395 (+)
  • chr6(ALT_REF_LOCI_2):3,616,757-3,619,145 (+)
  • chr6(ALT_REF_LOCI_4):3,483,386-3,485,774 (+)
  • chr6(ALT_REF_LOCI_5):3,520,426-3,522,812 (+)
  • chr6(ALT_REF_LOCI_6):3,401,606-3,403,994 (+)
  • chr6(ALT_REF_LOCI_7):3,494,612-3,497,000 (+)

Genomic View for RNF5 Gene

Genes around RNF5 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RNF5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RNF5 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

Proteins for RNF5 Gene

  • Protein details for RNF5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q99942-RNF5_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF5
    Protein Accession:
    Q99942
    Secondary Accessions:
    • A2BFI6
    • B2R4K3
    • Q0VDB7
    • Q9UMQ2

    Protein attributes for RNF5 Gene

    Size:
    180 amino acids
    Molecular mass:
    19881 Da
    Quaternary structure:
    • Interacts with PXN. Interacts with Salmonella typhimurium sopA. Interacts with JKAMP. Interacts with STING1; the interaction of endogenous proteins is dependent on viral infection.

neXtProt entry for RNF5 Gene

Post-translational modifications for RNF5 Gene

  • Ubiquitination at Lys68, Lys75, Lys86, and Lys93
  • Modification sites at PhosphoSitePlus

Other Protein References for RNF5 Gene

ENSEMBL proteins:
REFSEQ proteins:

Antibodies for research

  • Abcam antibodies for RNF5
  • Boster Bio Antibodies for RNF5
  • Santa Cruz Biotechnology (SCBT) Antibodies for RNF5

No data available for DME Specific Peptides for RNF5 Gene

Domains & Families for RNF5 Gene

Gene Families for RNF5 Gene

Protein Domains for RNF5 Gene

InterPro:
Blocks:
  • Zn-finger, RING

Suggested Antigen Peptide Sequences for RNF5 Gene

GenScript: Design optimal peptide antigens:
  • Ram1 homolog (RNF5_HUMAN)
genes like me logo Genes that share domains with RNF5: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for RNF5 Gene

Function for RNF5 Gene

Molecular function for RNF5 Gene

UniProtKB/Swiss-Prot Function:
Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of STING1 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27;.
GENATLAS Biochemistry:
RING zinc-finger protein 5,widely expressed,homolog of a putative zinc finger gene from C elegans,same as D6S115E

Enzyme Numbers (IUBMB) for RNF5 Gene

Phenotypes From GWAS Catalog for RNF5 Gene

Gene Ontology (GO) - Molecular Function for RNF5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004842 ubiquitin-protein transferase activity IDA 19269966
GO:0005515 protein binding IPI 12861019
GO:0008270 zinc ion binding TAS 9533025
GO:0016740 transferase activity IEA --
GO:0042802 identical protein binding IPI 25416956
genes like me logo Genes that share ontologies with RNF5: view
genes like me logo Genes that share phenotypes with RNF5: view

Animal Models for RNF5 Gene

MGI Knock Outs for RNF5:
  • Rnf5 Rnf5<tm1Zron>
  • Rnf5 Rnf5<tm1.1(KOMP)Vlcg>

Animal Models for research

  • Taconic Biosciences Mouse Models for RNF5

miRNA for RNF5 Gene

miRTarBase miRNAs that target RNF5

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF5

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RNF5 Gene

Localization for RNF5 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF5 Gene

Membrane. Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane. Note=Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RNF5 gene
Compartment Confidence
mitochondrion 5
nucleus 4
endoplasmic reticulum 4
plasma membrane 2
peroxisome 2
cytosol 2
lysosome 2
extracellular 1
cytoskeleton 1
endosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Endoplasmic reticulum (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for RNF5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005739 mitochondrion IEA --
GO:0005783 endoplasmic reticulum IDA --
GO:0005789 endoplasmic reticulum membrane TAS --
GO:0016020 membrane IEA --
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with RNF5: view

Pathways & Interactions for RNF5 Gene

genes like me logo Genes that share pathways with RNF5: view

Pathways by source for RNF5 Gene

2 GeneGo (Thomson Reuters) pathways for RNF5 Gene
  • Regulation of degradation of deltaF508 CFTR in CF
  • Regulation of degradation of wt-CFTR

UniProtKB/Swiss-Prot Q99942-RNF5_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for RNF5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006511 ubiquitin-dependent protein catabolic process IBA 21873635
GO:0009617 response to bacterium IEA --
GO:0010507 negative regulation of autophagy IEA --
GO:0016567 protein ubiquitination IEA --
GO:0030433 ubiquitin-dependent ERAD pathway IGI 24019521
genes like me logo Genes that share ontologies with RNF5: view

No data available for SIGNOR curated interactions for RNF5 Gene

Drugs & Compounds for RNF5 Gene

(3) Drugs for RNF5 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine monophosphate Approved, Investigational Nutra 0
Phosphate Experimental Pharma 0
ATP Investigational Nutra 0

(1) Additional Compounds for RNF5 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • [(HO)2P(O)OP(O)(OH)2]
  • Acide diphosphorique
  • Diphosphorsaeure
  • H4P2O7
  • Pyrophosphoric acid
14000-31-8
genes like me logo Genes that share compounds with RNF5: view

Transcripts for RNF5 Gene

mRNA/cDNA for RNF5 Gene

1 REFSEQ mRNAs :
17 NCBI additional mRNA sequence :
2 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF5

Alternative Splicing Database (ASD) splice patterns (SP) for RNF5 Gene

No ASD Table

Relevant External Links for RNF5 Gene

GeneLoc Exon Structure for
RNF5

Expression for RNF5 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RNF5 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

  • Lung (Respiratory System)

Protein differential expression in normal tissues from HIPED for RNF5 Gene

This gene is overexpressed in Breast (20.4), Adrenal (14.5), Bone (8.9), and Frontal cortex (8.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for RNF5 Gene



Protein tissue co-expression partners for RNF5 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for RNF5

SOURCE GeneReport for Unigene cluster for RNF5 Gene:

Hs.731774

mRNA Expression by UniProt/SwissProt for RNF5 Gene:

Q99942-RNF5_HUMAN
Tissue specificity: Widely expressed.

Evidence on tissue expression from TISSUES for RNF5 Gene

  • Nervous system(4.6)
  • Liver(4.5)
  • Lung(4.4)
  • Muscle(3)
  • Kidney(2.8)
  • Heart(2.2)
  • Spleen(2.1)
  • Intestine(2.1)
  • Skin(2)
  • Lymph node(2)
genes like me logo Genes that share expression patterns with RNF5: view

Primer products for research

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for RNF5 Gene

Orthologs for RNF5 Gene

This gene was present in the common ancestor of animals.

Orthologs for RNF5 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia RNF5 29 30
  • 99.63 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia RNF5 29 30
  • 95.19 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia RNF5 29 30
  • 94.81 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Rnf5 29 16 30
  • 91.67 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Rnf5 29
  • 90.45 (n)
Oppossum
(Monodelphis domestica)
Mammalia RNF5 30
  • 88 (a)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia RNF5 30
  • 64 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia rnf5 29
  • 73.71 (n)
African clawed frog
(Xenopus laevis)
Amphibia Xl.15397 29
Zebrafish
(Danio rerio)
Actinopterygii LOC100537822 29
  • 68.21 (n)
RNF5 30
  • 66 (a)
OneToOne
Dr.13010 29
Fruit Fly
(Drosophila melanogaster)
Insecta CG32847 30
  • 38 (a)
ManyToMany
CG8974 30 31
  • 33 (a)
ManyToMany
CG32581 30
  • 33 (a)
ManyToMany
Worm
(Caenorhabditis elegans)
Secernentea rnf-5 30 31
  • 34 (a)
OneToMany
Species where no ortholog for RNF5 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Platypus (Ornithorhynchus anatinus)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for RNF5 Gene

ENSEMBL:
Gene Tree for RNF5 (if available)
TreeFam:
Gene Tree for RNF5 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RNF5: view image
Alliance of Genome Resources:
Additional Orthologs for RNF5

Paralogs for RNF5 Gene

Paralogs for RNF5 Gene

(2) SIMAP similar genes for RNF5 Gene using alignment to 2 proteins:

  • RNF5_HUMAN
  • E7EM95_HUMAN

Pseudogenes.org Pseudogenes for RNF5 Gene

genes like me logo Genes that share paralogs with RNF5: view

Variants for RNF5 Gene

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for RNF5 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for RNF5 Gene

Variant ID Type Subtype PubMed ID
dgv10404n54 CNV loss 21841781
dgv10463n54 CNV loss 21841781
dgv10472n54 CNV loss 21841781
esv24778 CNV loss 19812545
esv2759415 CNV gain+loss 17122850
nsv1073969 CNV deletion 25765185
nsv1112900 CNV deletion 24896259
nsv1126749 CNV deletion 24896259
nsv602104 CNV loss 21841781
nsv830629 CNV loss 17160897

Variation tolerance for RNF5 Gene

Residual Variation Intolerance Score: 34.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.06; 21.57% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RNF5 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RNF5
Leiden Open Variation Database (LOVD)
RNF5

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for RNF5 Gene

Disorders for RNF5 Gene

MalaCards: The human disease database

(1) MalaCards diseases for RNF5 Gene - From: COP and GCD

Disorder Aliases PubMed IDs
cystic fibrosis
  • cf
- elite association - COSMIC cancer census association via MalaCards
Search RNF5 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for RNF5

Human Genome Epidemiology Navigator
(HuGE)
Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with RNF5: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RNF5 Gene

Publications for RNF5 Gene

  1. RNF5, a RING finger protein that regulates cell motility by targeting paxillin ubiquitination and altered localization. (PMID: 12861019) Didier C … Ronai Z (Molecular and cellular biology 2003) 3 4 22
  2. Cloning, expression and mapping of a novel RING-finger gene (RNF5), a human homologue of a putative zinc-finger gene from Caenorhabditis elegans. (PMID: 9533025) Kyushiki H … Horie M (Cytogenetics and cell genetics 1997) 2 3 4
  3. Regulation of endoplasmic reticulum-associated degradation by RNF5-dependent ubiquitination of JNK-associated membrane protein (JAMP). (PMID: 19269966) Tcherpakov M … Ronai ZA (The Journal of biological chemistry 2009) 3 4
  4. The ubiquitin ligase RNF5 regulates antiviral responses by mediating degradation of the adaptor protein MITA. (PMID: 19285439) Zhong B … Shu HB (Immunity 2009) 3 4
  5. High-density SNP screening of the major histocompatibility complex in systemic lupus erythematosus demonstrates strong evidence for independent susceptibility regions. (PMID: 19851445) Barcellos LF … Criswell LA (PLoS genetics 2009) 3 40

Products for RNF5 Gene

Sources for RNF5 Gene