The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent... See more...

Aliases for RNF4 Gene

Aliases for RNF4 Gene

  • Ring Finger Protein 4 2 3 5
  • SNURF 2 3 4
  • RING-Type E3 Ubiquitin Transferase RNF4 3 4
  • E3 Ubiquitin-Protein Ligase RNF4 3 4
  • RES4-26 2 3
  • SLX5 2 3
  • Small Nuclear RING Finger Protein 3
  • Small Nuclear Ring Finger Protein 4
  • E3 Ubiquitin Ligase RNF4 3
  • RING Finger Protein 4 4
  • Protein SNURF 4
  • EC 2.3.2.27 4
  • RNF4 5

External Ids for RNF4 Gene

Previous GeneCards Identifiers for RNF4 Gene

  • GC04P002465
  • GC04P002337
  • GC04P002432
  • GC04P002438
  • GC04P002463
  • GC04P002410

Summaries for RNF4 Gene

Entrez Gene Summary for RNF4 Gene

  • The protein encoded by this gene contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.[provided by RefSeq, Jul 2010]

GeneCards Summary for RNF4 Gene

RNF4 (Ring Finger Protein 4) is a Protein Coding gene. Diseases associated with RNF4 include Gordon Holmes Syndrome. Among its related pathways are Androgen receptor signaling pathway and Innate Immune System. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and ligase activity.

UniProtKB/Swiss-Prot Summary for RNF4 Gene

  • E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation.

Gene Wiki entry for RNF4 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RNF4 Gene

Genomics for RNF4 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for RNF4 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RNF4 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RNF4

Top Transcription factor binding sites by QIAGEN in the RNF4 gene promoter:
  • HEN1
  • HNF-4alpha1
  • HNF-4alpha2
  • NF-1
  • Nkx6-1
  • Pax-2
  • Pax-2a
  • PPAR-gamma1
  • PPAR-gamma2

Genomic Locations for RNF4 Gene

Genomic Locations for RNF4 Gene
chr4:2,462,220-2,625,320
(GRCh38/hg38)
Size:
163,101 bases
Orientation:
Plus strand
chr4:2,463,947-2,627,047
(GRCh37/hg19)
Size:
163,101 bases
Orientation:
Plus strand

Genomic View for RNF4 Gene

Genes around RNF4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RNF4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RNF4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF4 Gene

Proteins for RNF4 Gene

  • Protein details for RNF4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P78317-RNF4_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF4
    Protein Accession:
    P78317
    Secondary Accessions:
    • B2R6D6
    • D6RF58
    • Q49AR8

    Protein attributes for RNF4 Gene

    Size:
    190 amino acids
    Molecular mass:
    21319 Da
    Quaternary structure:
    • Homodimer (via RING-type zinc finger domain). Interacts with GSC2 (By similarity). Interacts with AR/the androgen receptor and TBP (By similarity). Interacts with TCF20 (By similarity). Interacts with PATZ1. Interacts with TRPS1; negatively regulates TRPS1 transcriptional repressor activity. Interacts with PML; SUMO1-dependent. Interacts with PML; SUMO2-dependent. Interacts with PARP1. Interacts with PML (isoform PML-1, isoform PML-2, isoform PML-3, isoform PML-4, isoform PML-5 and isoform PML-6).

    Three dimensional structures from OCA and Proteopedia for RNF4 Gene

    Alternative splice isoforms for RNF4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RNF4 Gene

Post-translational modifications for RNF4 Gene

No data available for DME Specific Peptides for RNF4 Gene

Domains & Families for RNF4 Gene

Gene Families for RNF4 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for RNF4 Gene

InterPro:
Blocks:
  • Zn-finger, RING
ProtoNet:

Suggested Antigen Peptide Sequences for RNF4 Gene

GenScript: Design optimal peptide antigens:
  • Ring finger protein 4, isoform CRA_a (D3DVQ6_HUMAN)
  • Small nuclear ring finger protein (RNF4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P78317

UniProtKB/Swiss-Prot:

RNF4_HUMAN :
  • The SUMO interaction motifs (SIMs) mediates the binding to polysumoylated substrate.
Domain:
  • The SUMO interaction motifs (SIMs) mediates the binding to polysumoylated substrate.
  • The RING-type zinc finger domain is required for the ubiquitination of polysumoylated substrates.
genes like me logo Genes that share domains with RNF4: view

Function for RNF4 Gene

Molecular function for RNF4 Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27;.
GENATLAS Biochemistry:
RING finger protein 4,abundantly expressed in fetal tissues,involved in the protein and DNA association in the nucleus of the cell

Enzyme Numbers (IUBMB) for RNF4 Gene

Phenotypes From GWAS Catalog for RNF4 Gene

Gene Ontology (GO) - Molecular Function for RNF4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding ISS --
GO:0003713 transcription coactivator activity NAS 15572661
GO:0004842 ubiquitin-protein transferase activity ISS --
GO:0005515 protein binding IPI 12885770
GO:0008134 transcription factor binding IBA 21873635
genes like me logo Genes that share ontologies with RNF4: view
genes like me logo Genes that share phenotypes with RNF4: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF4

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RNF4 Gene

Localization for RNF4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF4 Gene

Cytoplasm. Nucleus. Nucleus, PML body. Nucleus, nucleoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RNF4 gene
Compartment Confidence
nucleus 5
cytoskeleton 4
cytosol 3
plasma membrane 1
extracellular 1
mitochondrion 1
peroxisome 1
endoplasmic reticulum 1
endosome 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nuclear bodies (3)
  • Nucleoplasm (3)
  • Microtubule ends (1)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for RNF4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA,IDA 12885770
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IEA,IDA 12885770
GO:0016604 nuclear body IDA --
GO:0016605 colocalizes_with PML body IBA,IDA 20943951
genes like me logo Genes that share ontologies with RNF4: view

Pathways & Interactions for RNF4 Gene

genes like me logo Genes that share pathways with RNF4: view

UniProtKB/Swiss-Prot P78317-RNF4_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

SIGNOR curated interactions for RNF4 Gene

Activates:
Is inactivated by:

Gene Ontology (GO) - Biological Process for RNF4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016567 protein ubiquitination IEA --
GO:0030521 androgen receptor signaling pathway NAS 15572661
GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process IMP 18408734
GO:0045893 positive regulation of transcription, DNA-templated ISS --
GO:0045944 positive regulation of transcription by RNA polymerase II IDA 12885770
genes like me logo Genes that share ontologies with RNF4: view

Drugs & Compounds for RNF4 Gene

(3) Drugs for RNF4 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials

(1) Additional Compounds for RNF4 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with RNF4: view

Transcripts for RNF4 Gene

mRNA/cDNA for RNF4 Gene

3 REFSEQ mRNAs :
12 NCBI additional mRNA sequence :
22 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF4

Alternative Splicing Database (ASD) splice patterns (SP) for RNF4 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7 ^ 8 ^ 9a · 9b · 9c
SP1: -
SP2: -
SP3: - - - - - - -
SP4:
SP5:
SP6: -

Relevant External Links for RNF4 Gene

GeneLoc Exon Structure for
RNF4

Expression for RNF4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RNF4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

  • Thymus (Hematopoietic System)

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for RNF4 Gene



Transcriptomic regulation report from SPP (The Signaling Pathways Project) for RNF4

SOURCE GeneReport for Unigene cluster for RNF4 Gene:

Hs.66394

mRNA Expression by UniProt/SwissProt for RNF4 Gene:

P78317-RNF4_HUMAN
Tissue specificity: Widely expressed at low levels in many tissues; highly expressed in testis.

Evidence on tissue expression from TISSUES for RNF4 Gene

  • Nervous system(5)
  • Skin(4.6)
  • Kidney(3.3)
  • Blood(2.5)
  • Lung(2.5)
  • Intestine(2.5)
genes like me logo Genes that share expression patterns with RNF4: view

No data available for mRNA differential expression in normal tissues , Protein differential expression in normal tissues , Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for RNF4 Gene

Orthologs for RNF4 Gene

This gene was present in the common ancestor of chordates.

Orthologs for RNF4 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia RNF4 30 31
  • 99.47 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia RNF4 30 31
  • 89.12 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Rnf4 30 17 31
  • 88.95 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Rnf4 30
  • 88.59 (n)
Cow
(Bos Taurus)
Mammalia RNF4 30 31
  • 84.41 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia RNF4 31
  • 66 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia RNF4 31
  • 64 (a)
OneToOne
Chicken
(Gallus gallus)
Aves RNF4 30 31
  • 70.37 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia RNF4 31
  • 61 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia rnf4 30
  • 61.72 (n)
Str.2024 30
African clawed frog
(Xenopus laevis)
Amphibia Xl.5794 30
Zebrafish
(Danio rerio)
Actinopterygii im:7142942 30
  • 53.33 (n)
RNF4 31
  • 47 (a)
OneToOne
Dr.17303 30
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.8525 30
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.5143 31
  • 23 (a)
OneToOne
Species where no ortholog for RNF4 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for RNF4 Gene

ENSEMBL:
Gene Tree for RNF4 (if available)
TreeFam:
Gene Tree for RNF4 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RNF4: view image

Paralogs for RNF4 Gene

(4) SIMAP similar genes for RNF4 Gene using alignment to 11 proteins:

  • RNF4_HUMAN
  • D3DVQ6_HUMAN
  • D6R9E0_HUMAN
  • D6RA71_HUMAN
  • D6RAD8_HUMAN
  • D6RBQ7_HUMAN
  • D6RBZ1_HUMAN
  • D6RJ08_HUMAN
  • D6RJF5_HUMAN
  • F5H1U2_HUMAN
  • H0YAI5_HUMAN

Pseudogenes.org Pseudogenes for RNF4 Gene

genes like me logo Genes that share paralogs with RNF4: view

No data available for Paralogs for RNF4 Gene

Variants for RNF4 Gene

Additional dbSNP identifiers (rs#s) for RNF4 Gene

Structural Variations from Database of Genomic Variants (DGV) for RNF4 Gene

Variant ID Type Subtype PubMed ID
esv25411 CNV loss 19812545
esv2726835 CNV deletion 23290073
esv2726853 CNV deletion 23290073
esv2823 CNV loss 18987735
esv3599403 CNV loss 21293372
nsv1009347 CNV gain 25217958
nsv1010352 CNV gain 25217958
nsv1123740 CNV deletion 24896259
nsv290753 CNV deletion 16902084
nsv518472 CNV loss 19592680
nsv967718 CNV duplication 23825009

Variation tolerance for RNF4 Gene

Residual Variation Intolerance Score: 82% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.85; 17.82% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RNF4 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RNF4

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for RNF4 Gene

Disorders for RNF4 Gene

MalaCards: The human disease database

(1) MalaCards diseases for RNF4 Gene - From: GeneCards

Disorder Aliases PubMed IDs
gordon holmes syndrome
  • gdhs
- elite association - COSMIC cancer census association via MalaCards
Search RNF4 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for RNF4

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with RNF4: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RNF4 Gene

Publications for RNF4 Gene

  1. RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for arsenic-induced PML degradation. (PMID: 18408734) Tatham MH … Hay RT (Nature cell biology 2008) 3 4 23
  2. SUMO-1 promotes association of SNURF (RNF4) with PML nuclear bodies. (PMID: 15707587) Häkli M … Palvimo JJ (Experimental cell research 2005) 3 4 23
  3. The RING finger protein RNF4, a co-regulator of transcription, interacts with the TRPS1 transcription factor. (PMID: 12885770) Kaiser FJ … Lüdecke HJ (The Journal of biological chemistry 2003) 3 4 23
  4. Identification and characterization of a novel RING-finger gene (RNF4) mapping at 4p16.3. (PMID: 9479498) Chiariotti L … Bruni CB (Genomics 1998) 2 3 4
  5. Requirement of PML SUMO interacting motif for RNF4- or arsenic trioxide-induced degradation of nuclear PML isoforms. (PMID: 23028697) Maroui MA … Chelbi-Alix MK (PloS one 2012) 3 4

Products for RNF4 Gene

Sources for RNF4 Gene