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Aliases for RNF185 Gene

Aliases for RNF185 Gene

  • Ring Finger Protein 185 2 3 5
  • RING-Type E3 Ubiquitin Transferase RNF185 3 4
  • E3 Ubiquitin-Protein Ligase RNF185 3
  • Hypothetical Protein FLJ38628 2
  • RING Finger Protein 185 4
  • BSK65-MONO1 3
  • BSK65-MONO2 3
  • BSK65-PANC1 3
  • BSK65-PANC2 3
  • BSK65-TEST1 3
  • BSK65-TEST2 3
  • BSK65-TEST3 3
  • EC 2.3.2.27 4

External Ids for RNF185 Gene

Previous GeneCards Identifiers for RNF185 Gene

  • GC22P029881
  • GC22P031556
  • GC22P014517

Summaries for RNF185 Gene

GeneCards Summary for RNF185 Gene

RNF185 (Ring Finger Protein 185) is a Protein Coding gene. Among its related pathways are Calnexin/calreticulin cycle and Protein processing in endoplasmic reticulum. Gene Ontology (GO) annotations related to this gene include ligase activity. An important paralog of this gene is SYNJ2.

UniProtKB/Swiss-Prot for RNF185 Gene

  • E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1 (PubMed:21931693). Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed:27485036). Protects cells from ER stress-induced apoptosis (PubMed:27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed:24019521). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed:24019521).

Additional gene information for RNF185 Gene

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RNF185 Gene

Genomics for RNF185 Gene

GeneHancer (GH) Regulatory Elements for RNF185 Gene

Promoters and enhancers for RNF185 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH22J031159 Promoter/Enhancer 2 EPDnew Ensembl ENCODE 650.7 +0.5 499 2.4 PKNOX1 FOXA2 ARNT ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF766 MIR3928 RNF185 TUG1 MORC2 SF3A1 PES1 PRR14L DEPDC5 TBC1D10A ENSG00000268812
GH22J031162 Enhancer 0.5 ENCODE 650.7 +2.0 1994 0.2 SP1 RXRA HNF4A GABPA RNF185 PIR51329
GH22J031137 Promoter 0.5 EPDnew 650.3 -22.4 -22378 0.1 RNF185 PLA2G3 LOC105372995
GH22J031209 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 24.3 +51.5 51463 4.3 HDGF PKNOX1 FOXA2 MLX ZFP64 ARID4B SIN3A DMAP1 YY1 SLC30A9 LIMK2 RNF185 SF3A1 TUG1 ENSG00000214125 PLA2G3 PRR14L MORC2 TBC1D10A PIK3IP1
GH22J031097 Promoter/Enhancer 2.4 EPDnew Ensembl ENCODE dbSUPER 22.4 -57.1 -57101 10.5 PKNOX1 FOXA2 ARNT ARID4B SIN3A YY1 ZNF143 FOS DEK SP3 TUG1 MORC2 RNF185 SF3A1 PRR14L DEPDC5 PES1 SELENOM PATZ1 RNF215
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around RNF185 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the RNF185 gene promoter:

Genomic Locations for RNF185 Gene

Genomic Locations for RNF185 Gene
chr22:31,160,152-31,207,019
(GRCh38/hg38)
Size:
46,868 bases
Orientation:
Plus strand
chr22:31,556,138-31,603,005
(GRCh37/hg19)

Genomic View for RNF185 Gene

Genes around RNF185 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RNF185 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RNF185 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF185 Gene

Proteins for RNF185 Gene

  • Protein details for RNF185 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96GF1-RN185_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF185
    Protein Accession:
    Q96GF1
    Secondary Accessions:
    • A8K5C1
    • A9X3T8
    • Q8N900

    Protein attributes for RNF185 Gene

    Size:
    192 amino acids
    Molecular mass:
    20459 Da
    Quaternary structure:
    • Interacts with ATG5 and BNIP1.

    Alternative splice isoforms for RNF185 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RNF185 Gene

Post-translational modifications for RNF185 Gene

  • Ubiquitination at posLast=8787 and Lys80
  • Modification sites at PhosphoSitePlus

Other Protein References for RNF185 Gene

No data available for DME Specific Peptides for RNF185 Gene

Domains & Families for RNF185 Gene

Gene Families for RNF185 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins
  • Predicted membrane proteins

Protein Domains for RNF185 Gene

Graphical View of Domain Structure for InterPro Entry

Q96GF1

UniProtKB/Swiss-Prot:

RN185_HUMAN :
  • The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.
Domain:
  • The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.
genes like me logo Genes that share domains with RNF185: view

Function for RNF185 Gene

Molecular function for RNF185 Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating Lys-63-linked polyubiquitination of BNIP1 (PubMed:21931693). Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation (PubMed:27485036). Protects cells from ER stress-induced apoptosis (PubMed:27485036). Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway (PubMed:24019521). Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2 (PubMed:24019521).
UniProtKB/Swiss-Prot CatalyticActivity:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
UniProtKB/Swiss-Prot Induction:
Up-regulated by unfolded protein response (UPR) and endoplasmic reticulum (ER) stress triggered by thapsigargin or tunicamycin.

Enzyme Numbers (IUBMB) for RNF185 Gene

Phenotypes From GWAS Catalog for RNF185 Gene

Gene Ontology (GO) - Molecular Function for RNF185 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 19549727
GO:0016740 transferase activity IEA --
GO:0044390 ubiquitin-like protein conjugating enzyme binding IPI 24019521
GO:0046872 metal ion binding IEA --
GO:0061630 ubiquitin protein ligase activity IDA 24019521
genes like me logo Genes that share ontologies with RNF185: view
genes like me logo Genes that share phenotypes with RNF185: view

Animal Model Products

Clone Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RNF185 Gene

Localization for RNF185 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF185 Gene

Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RNF185 gene
Compartment Confidence
endoplasmic reticulum 5
mitochondrion 3
nucleus 3
peroxisome 2

Gene Ontology (GO) - Cellular Components for RNF185 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005739 mitochondrion IEA --
GO:0005741 mitochondrial outer membrane IEA --
GO:0005783 endoplasmic reticulum IDA 24019521
GO:0005789 endoplasmic reticulum membrane TAS,NAS --
GO:0016020 membrane IEA --
genes like me logo Genes that share ontologies with RNF185: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for RNF185 Gene

Pathways & Interactions for RNF185 Gene

genes like me logo Genes that share pathways with RNF185: view

UniProtKB/Swiss-Prot Q96GF1-RN185_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for RNF185 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006914 autophagy IEA --
GO:0016567 protein ubiquitination IEA --
GO:0030433 ubiquitin-dependent ERAD pathway IMP 24019521
GO:0036503 ERAD pathway IGI 24019521
GO:0051865 protein autoubiquitination IDA 24019521
genes like me logo Genes that share ontologies with RNF185: view

No data available for SIGNOR curated interactions for RNF185 Gene

Drugs & Compounds for RNF185 Gene

No Compound Related Data Available

Transcripts for RNF185 Gene

mRNA/cDNA for RNF185 Gene

Unigene Clusters for RNF185 Gene

Ring finger protein 185:
Representative Sequences:

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for RNF185 Gene

ExUns: 1a · 1b · 1c · 1d · 1e · 1f · 1g ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b · 10c ^ 11a · 11b ·
SP1: - - - - - - - - -
SP2: - - - - - - - - - -
SP3: - - - - - -
SP4: -
SP5: - - - - - - - - - - - -
SP6: -
SP7: - - -
SP8: - - - - - - -
SP9: - - - - - - -
SP10: - - - - - - -
SP11: - - - - - - -
SP12: - - - - - -
SP13: - - - - - - -
SP14: - - - - - - -
SP15: - - - - - - - - - - - - - -
SP16: - - - - - -
SP17:
SP18: - -

ExUns: 11c · 11d
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:
SP15:
SP16:
SP17:
SP18:

Relevant External Links for RNF185 Gene

GeneLoc Exon Structure for
RNF185
ECgene alternative splicing isoforms for
RNF185

Expression for RNF185 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for RNF185 Gene

Protein differential expression in normal tissues from HIPED for RNF185 Gene

This gene is overexpressed in Bone (18.5), Pancreas (8.1), and Lung (6.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for RNF185 Gene



Protein tissue co-expression partners for RNF185 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of RNF185 Gene:

RNF185

SOURCE GeneReport for Unigene cluster for RNF185 Gene:

Hs.517553

mRNA Expression by UniProt/SwissProt for RNF185 Gene:

Q96GF1-RN185_HUMAN
Tissue specificity: Ubiquitously expressed.

Evidence on tissue expression from TISSUES for RNF185 Gene

  • Nervous system(4.6)
  • Intestine(4.2)
  • Lung(4.2)
  • Muscle(4.2)
  • Pancreas(4.2)
genes like me logo Genes that share expression patterns with RNF185: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for RNF185 Gene

Orthologs for RNF185 Gene

This gene was present in the common ancestor of animals.

Orthologs for RNF185 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RNF185 34 33
  • 100 (n)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia RNF185 34
  • 95 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RNF185 34
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RNF185 34 33
  • 94.62 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Rnf185 16 34 33
  • 93.06 (n)
cow
(Bos Taurus)
Mammalia RNF185 34 33
  • 92.71 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rnf185 33
  • 92.36 (n)
chicken
(Gallus gallus)
Aves RNF185 34 33
  • 82.12 (n)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.13633 33
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.4501 33
zebrafish
(Danio rerio)
Actinopterygii rnf185 34 33
  • 73.26 (n)
OneToOne
wufk32c04 33
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.8264 33
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP006041 33
  • 57.92 (n)
fruit fly
(Drosophila melanogaster)
Insecta CG8974 34 33
  • 57.46 (n)
ManyToMany
CG32847 34
  • 43 (a)
ManyToMany
CG32581 34
  • 37 (a)
ManyToMany
worm
(Caenorhabditis elegans)
Secernentea rnf-5 34 33
  • 54.64 (n)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea Cin.2160 33
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.2160 33
Species where no ortholog for RNF185 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RNF185 Gene

ENSEMBL:
Gene Tree for RNF185 (if available)
TreeFam:
Gene Tree for RNF185 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RNF185: view image

Paralogs for RNF185 Gene

Paralogs for RNF185 Gene

(1) SIMAP similar genes for RNF185 Gene using alignment to 2 proteins:

  • RN185_HUMAN
  • B4DMD6_HUMAN

Pseudogenes.org Pseudogenes for RNF185 Gene

genes like me logo Genes that share paralogs with RNF185: view

Variants for RNF185 Gene

Sequence variations from dbSNP and Humsavar for RNF185 Gene

SNP ID Clin Chr 22 pos Variation AA Info Type
rs1000000149 -- 31,176,778(+) G/A intron_variant
rs1000072243 -- 31,164,249(+) G/A intron_variant
rs1000130830 -- 31,183,084(+) C/T intron_variant
rs1000130857 -- 31,170,314(+) G/A intron_variant
rs1000269601 -- 31,203,233(+) C/T intron_variant

Structural Variations from Database of Genomic Variants (DGV) for RNF185 Gene

Variant ID Type Subtype PubMed ID
esv3568357 CNV loss 25503493
nsv1110864 OTHER inversion 24896259
nsv459871 CNV loss 19166990
nsv507933 OTHER sequence alteration 20534489
nsv588893 CNV loss 21841781
nsv979669 CNV duplication 23825009

Variation tolerance for RNF185 Gene

Residual Variation Intolerance Score: 47.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.29; 6.48% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RNF185 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RNF185

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF185 Gene

Disorders for RNF185 Gene

Additional Disease Information for RNF185

No disorders were found for RNF185 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for RNF185 Gene

Publications for RNF185 Gene

  1. RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR). (PMID: 24019521) El Khouri E … Delaunay-Moisan A (The Journal of biological chemistry 2013) 3 4 58
  2. RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1. (PMID: 21931693) Tang F … Tang J (PloS one 2011) 3 4 58
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 58
  4. A genome annotation-driven approach to cloning the human ORFeome. (PMID: 15461802) Collins JE … Dunham I (Genome biology 2004) 3 4 58
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4 58

Products for RNF185 Gene

Sources for RNF185 Gene

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