Aliases for RNF166 Gene

Aliases for RNF166 Gene

  • Ring Finger Protein 166 2 3 5
  • RING-Type E3 Ubiquitin Transferase RNF166 3 4
  • E3 Ubiquitin-Protein Ligase RNF166 3 4
  • RING Finger Protein 166 4
  • EC 2.3.2.27 4
  • MGC14381 2
  • MGC2647 2
  • RNF166 5

External Ids for RNF166 Gene

Previous GeneCards Identifiers for RNF166 Gene

  • GC16M088508
  • GC16M087290
  • GC16M088762
  • GC16M074457

Summaries for RNF166 Gene

GeneCards Summary for RNF166 Gene

RNF166 (Ring Finger Protein 166) is a Protein Coding gene. Diseases associated with RNF166 include Generalized Epilepsy With Febrile Seizures Plus, Type 9 and Myopathy, Congenital, Compton-North. An important paralog of this gene is RNF114.

UniProtKB/Swiss-Prot Summary for RNF166 Gene

  • E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates (PubMed:27880896). In turn, participates in different biological processes including interferon production or autophagy (PubMed:26456228, PubMed:27880896). Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6 (PubMed:26456228). Plays also a role in the early recruitment of autophagy adapters to bacteria (PubMed:27880896). Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication (PubMed:27880896).

Additional gene information for RNF166 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RNF166 Gene

Genomics for RNF166 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for RNF166 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RNF166 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RNF166

Top Transcription factor binding sites by QIAGEN in the RNF166 gene promoter:
  • CREB
  • deltaCREB
  • E47
  • GR
  • GR-alpha
  • ITF-2
  • Olf-1
  • Pax-5
  • Tal-1beta

Genomic Locations for RNF166 Gene

Genomic Locations for RNF166 Gene
chr16:88,696,499-88,706,421
(GRCh38/hg38)
Size:
9,923 bases
Orientation:
Minus strand
chr16:88,762,903-88,772,829
(GRCh37/hg19)
Size:
9,927 bases
Orientation:
Minus strand

Genomic View for RNF166 Gene

Genes around RNF166 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RNF166 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RNF166 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF166 Gene

Proteins for RNF166 Gene

  • Protein details for RNF166 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q96A37-RN166_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF166
    Protein Accession:
    Q96A37
    Secondary Accessions:
    • B3KQ03
    • D3DX75
    • H3BTU8
    • Q96DM0

    Protein attributes for RNF166 Gene

    Size:
    237 amino acids
    Molecular mass:
    26122 Da
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for RNF166 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RNF166 Gene

Post-translational modifications for RNF166 Gene

  • Ubiquitination at Lys129 and Lys167
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for RNF166 Gene

Domains & Families for RNF166 Gene

Gene Families for RNF166 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for RNF166 Gene

InterPro:
Blocks:
  • Zn-finger, RING
  • Ubiquitin interacting motif
ProtoNet:

Suggested Antigen Peptide Sequences for RNF166 Gene

GenScript: Design optimal peptide antigens:
  • RING finger protein 166 (RN166_HUMAN)
genes like me logo Genes that share domains with RNF166: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for RNF166 Gene

Function for RNF166 Gene

Molecular function for RNF166 Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates (PubMed:27880896). In turn, participates in different biological processes including interferon production or autophagy (PubMed:26456228, PubMed:27880896). Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6 (PubMed:26456228). Plays also a role in the early recruitment of autophagy adapters to bacteria (PubMed:27880896). Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication (PubMed:27880896).
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269|PubMed:27880896};.

Enzyme Numbers (IUBMB) for RNF166 Gene

Phenotypes From GWAS Catalog for RNF166 Gene

Gene Ontology (GO) - Molecular Function for RNF166 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 19549727
GO:0016740 transferase activity IEA --
GO:0046872 metal ion binding IEA --
GO:0061630 ubiquitin protein ligase activity IBA 21873635
genes like me logo Genes that share ontologies with RNF166: view
genes like me logo Genes that share phenotypes with RNF166: view

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF166

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RNF166 Gene

Localization for RNF166 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF166 Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RNF166 gene
Compartment Confidence
nucleus 4
cytosol 3
plasma membrane 1
mitochondrion 1
peroxisome 1
endoplasmic reticulum 1
endosome 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (2)
  • Nucleoplasm (2)
  • Plasma membrane (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for RNF166 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA --
genes like me logo Genes that share ontologies with RNF166: view

Pathways & Interactions for RNF166 Gene

PathCards logo

SuperPathways for RNF166 Gene

No Data Available

UniProtKB/Swiss-Prot Q96A37-RN166_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for RNF166 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000209 protein polyubiquitination IBA 21873635
GO:0002376 immune system process IEA --
GO:0006511 ubiquitin-dependent protein catabolic process IBA 21873635
GO:0006914 autophagy IEA --
GO:0016567 protein ubiquitination IEA --
genes like me logo Genes that share ontologies with RNF166: view

No data available for Pathways by source and SIGNOR curated interactions for RNF166 Gene

Drugs & Compounds for RNF166 Gene

No Compound Related Data Available

Transcripts for RNF166 Gene

mRNA/cDNA for RNF166 Gene

3 REFSEQ mRNAs :
9 NCBI additional mRNA sequence :
11 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF166

Alternative Splicing Database (ASD) splice patterns (SP) for RNF166 Gene

ExUns: 1 ^ 2a · 2b · 2c · 2d · 2e ^ 3a · 3b · 3c ^ 4 ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c · 9d
SP1: - - - - - - - -
SP2: - - - - - - - -
SP3: - - - -
SP4: - -
SP5:
SP6:
SP7: - -
SP8:

Relevant External Links for RNF166 Gene

GeneLoc Exon Structure for
RNF166

Expression for RNF166 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RNF166 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for RNF166 Gene

This gene is overexpressed in Whole Blood (x12.9).

Protein differential expression in normal tissues from HIPED for RNF166 Gene

This gene is overexpressed in Neutrophil (46.1), Peripheral blood mononuclear cells (12.3), and Blymphocyte (6.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for RNF166 Gene



Protein tissue co-expression partners for RNF166 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for RNF166

SOURCE GeneReport for Unigene cluster for RNF166 Gene:

Hs.513804

Evidence on tissue expression from TISSUES for RNF166 Gene

  • Nervous system(4.4)
  • Blood(4.3)
  • Intestine(4.3)
  • Eye(4)
genes like me logo Genes that share expression patterns with RNF166: view

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for RNF166 Gene

Orthologs for RNF166 Gene

This gene was present in the common ancestor of chordates.

Orthologs for RNF166 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia RNF166 30 31
  • 99.72 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia RNF166 30 31
  • 91.28 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Rnf166 30 17 31
  • 91.14 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Rnf166 30
  • 90.15 (n)
Dog
(Canis familiaris)
Mammalia RNF166 30 31
  • 90.01 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia RNF166 31
  • 88 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia RNF166 31
  • 86 (a)
OneToOne
Chicken
(Gallus gallus)
Aves RNF166 30 31
  • 83.69 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia RNF166 31
  • 75 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia rnf166 30
  • 77.12 (n)
Zebrafish
(Danio rerio)
Actinopterygii RNF166 (1 of 2) 31
  • 89 (a)
OneToMany
RNF166 (2 of 2) 31
  • 82 (a)
OneToMany
LOC100334524 30
  • 75.81 (n)
Species where no ortholog for RNF166 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for RNF166 Gene

ENSEMBL:
Gene Tree for RNF166 (if available)
TreeFam:
Gene Tree for RNF166 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RNF166: view image

Paralogs for RNF166 Gene

Paralogs for RNF166 Gene

(7) SIMAP similar genes for RNF166 Gene using alignment to 4 proteins:

  • RN166_HUMAN
  • H3BPZ9_HUMAN
  • H3BQ58_HUMAN
  • H3BTX7_HUMAN
genes like me logo Genes that share paralogs with RNF166: view

Variants for RNF166 Gene

Additional dbSNP identifiers (rs#s) for RNF166 Gene

Structural Variations from Database of Genomic Variants (DGV) for RNF166 Gene

Variant ID Type Subtype PubMed ID
dgv3064n100 CNV gain 25217958
nsv1065973 CNV loss 25217958
nsv1121921 CNV deletion 24896259
nsv471113 CNV loss 18288195
nsv471114 CNV gain 18288195
nsv482951 CNV loss 15286789
nsv827833 CNV gain 20364138
nsv833325 CNV loss 17160897
nsv833327 CNV loss 17160897
nsv952070 CNV deletion 24416366

Variation tolerance for RNF166 Gene

Residual Variation Intolerance Score: 76.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.41; 8.92% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RNF166 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RNF166

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for RNF166 Gene

Disorders for RNF166 Gene

MalaCards: The human disease database

(5) MalaCards diseases for RNF166 Gene - From: DISEASES

- elite association - COSMIC cancer census association via MalaCards
genes like me logo Genes that share disorders with RNF166: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RNF166 Gene

Publications for RNF166 Gene

  1. RNF166 Determines Recruitment of Adaptor Proteins during Antibacterial Autophagy. (PMID: 27880896) Heath RJ … Xavier RJ (Cell reports 2016) 2 3 4
  2. Ring finger protein 166 potentiates RNA virus-induced interferon-β production via enhancing the ubiquitination of TRAF3 and TRAF6. (PMID: 26456228) Chen HW … Chen DY (Scientific reports 2015) 3 4
  3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4
  5. A reference map of the human binary protein interactome. (PMID: 32296183) Luck K … Calderwood MA (Nature 2020) 3

Products for RNF166 Gene

Sources for RNF166 Gene