Aliases for RNF149 Gene

Aliases for RNF149 Gene

  • Ring Finger Protein 149 2 3 5
  • RING-Type E3 Ubiquitin Transferase RNF149 3 4
  • DNA Polymerase-Transactivated Protein 2 3 4
  • E3 Ubiquitin-Protein Ligase RNF149 3 4
  • DNAPTP2 3 4
  • RING Finger Protein 149 4
  • EC 4
  • FLJ90504 2
  • RNF149 5

External Ids for RNF149 Gene

Previous GeneCards Identifiers for RNF149 Gene

  • GC02M101513
  • GC02M101351
  • GC02M101258
  • GC02M101887
  • GC02M095656

Summaries for RNF149 Gene

GeneCards Summary for RNF149 Gene

RNF149 (Ring Finger Protein 149) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include ligase activity and ubiquitin-protein transferase activity. An important paralog of this gene is RNF150.

UniProtKB/Swiss-Prot Summary for RNF149 Gene

  • E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation.

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RNF149 Gene

Genomics for RNF149 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for RNF149 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH02J101305 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 281.6 +0.1 62 7.2 TBP ZBTB10 BCL11A MXD4 CEBPA MNT IKZF1 SMAD5 ETS1 IRF2 RNF149 HSALNG0017364 HSALNG0017365 LOC105373512 MIR5696 SNORD89 IL1R1 MAP4K4 RPL31 lnc-TBC1D8-5
GH02J101250 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE CraniofacialAtlas dbSUPER 27.8 +55.7 55676 4.1 TBP SMAD5 ETS1 HES1 POLR2A CTCF BRCA1 ELF1 NONO HOMEZ CNOT11 TBC1D8 lnc-TBC1D8-1 SNORD89 RNF149 lnc-TBC1D8-4
GH02J101327 Enhancer 1.1 FANTOM5 ENCODE 31.2 -20.8 -20773 4.2 MXD4 ZNF217 ELF1 HOMEZ THAP11 GABPA MBD2 SP1 SMAD4 SAP130 HSALNG0017366 RNF149 CREG2 SNORD89 MAP4K4 HSALNG0017368
GH02J101712 Enhancer 1 FANTOM5 ENCODE dbSUPER 24.7 -408.4 -408382 8.8 ZFP64 ZNF610 RBM22 SPI1 ELF1 NR2F1 HLF ZNF184 TBL1XR1 EED IL1R2 RNF149 MAP4K4 IL1R1 lnc-RFX8-3 piR-38051-158
GH02J101245 Enhancer 0.9 ENCODE dbSUPER 25.3 +62.6 62640 1.6 KDM6A SMAD4 SAP130 ETV4 GABPB1 RARA DMAP1 DRAP1 KAT7 SOX5 piR-59907-013 RNF149 SNORD89 TBC1D8 lnc-TBC1D8-1 RPL31
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RNF149 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RNF149

Top Transcription factor binding sites by QIAGEN in the RNF149 gene promoter:
  • AP-4
  • Cart-1
  • CP2
  • HEN1
  • HTF
  • LCR-F1
  • POU2F1
  • POU2F1a
  • POU3F2
  • RFX1

Genomic Locations for RNF149 Gene

Latest Assembly
37,489 bases
Minus strand

Previous Assembly
(GRCh37/hg19 by Entrez Gene)
33,101 bases
Minus strand

(GRCh37/hg19 by Ensembl)
37,483 bases
Minus strand

Genomic View for RNF149 Gene

Genes around RNF149 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RNF149 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RNF149 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF149 Gene

Proteins for RNF149 Gene

  • Protein details for RNF149 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    E3 ubiquitin-protein ligase RNF149
    Protein Accession:
    Secondary Accessions:
    • Q53S14
    • Q8N5I8
    • Q8NBY5
    • Q8WUU3

    Protein attributes for RNF149 Gene

    400 amino acids
    Molecular mass:
    43165 Da
    Quaternary structure:
    No Data Available

neXtProt entry for RNF149 Gene

Post-translational modifications for RNF149 Gene

  • Glycosylation at Asn52 and Asn145
  • Ubiquitination at Lys245 and Lys260
  • Modification sites at PhosphoSitePlus

Other Protein References for RNF149 Gene

No data available for DME Specific Peptides for RNF149 Gene

Domains & Families for RNF149 Gene

Gene Families for RNF149 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Predicted membrane proteins

Protein Domains for RNF149 Gene

  • Zn-finger, RING

Suggested Antigen Peptide Sequences for RNF149 Gene

GenScript: Design optimal peptide antigens:
  • RING finger protein 149 (RN149_HUMAN)

Graphical View of Domain Structure for InterPro Entry



  • The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
  • The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
genes like me logo Genes that share domains with RNF149: view

Function for RNF149 Gene

Molecular function for RNF149 Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=;.

Enzyme Numbers (IUBMB) for RNF149 Gene

Phenotypes From GWAS Catalog for RNF149 Gene

Gene Ontology (GO) - Molecular Function for RNF149 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016740 transferase activity IEA --
GO:0046872 metal ion binding IEA --
GO:0061630 ubiquitin protein ligase activity IEA,IBA 21873635
genes like me logo Genes that share ontologies with RNF149: view
genes like me logo Genes that share phenotypes with RNF149: view

miRNA for RNF149 Gene

miRTarBase miRNAs that target RNF149

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF149

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RNF149 Gene

Localization for RNF149 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF149 Gene

Membrane. Single-pass membrane protein.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RNF149 gene
Compartment Confidence
plasma membrane 3
extracellular 1
cytoskeleton 1
mitochondrion 1
nucleus 1
endoplasmic reticulum 1
endosome 1
cytosol 1
lysosome 1
golgi apparatus 0

Subcellular locations from the

Human Protein Atlas (HPA)
  • Plasma membrane (2)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for RNF149 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IBA 21873635
GO:0016020 membrane IEA,HDA 19946888
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with RNF149: view

Pathways & Interactions for RNF149 Gene

PathCards logo

SuperPathways for RNF149 Gene

No Data Available

UniProtKB/Swiss-Prot Q8NC42-RN149_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for RNF149 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006511 ubiquitin-dependent protein catabolic process IBA 21873635
GO:0016567 protein ubiquitination IEA --
GO:0031647 regulation of protein stability IEA --
GO:0035690 cellular response to drug IEA --
GO:0043409 negative regulation of MAPK cascade IEA --
genes like me logo Genes that share ontologies with RNF149: view

No data available for Pathways by source and SIGNOR curated interactions for RNF149 Gene

Drugs & Compounds for RNF149 Gene

No Compound Related Data Available

Transcripts for RNF149 Gene

mRNA/cDNA for RNF149 Gene

8 NCBI additional mRNA sequence :
6 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF149

Alternative Splicing Database (ASD) splice patterns (SP) for RNF149 Gene

No ASD Table

Relevant External Links for RNF149 Gene

GeneLoc Exon Structure for

Expression for RNF149 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RNF149 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for RNF149 Gene

This gene is overexpressed in Whole Blood (x17.6).

Protein differential expression in normal tissues from HIPED for RNF149 Gene

This gene is overexpressed in Neutrophil (44.3) and Monocytes (17.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for RNF149 Gene

Protein tissue co-expression partners for RNF149 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for RNF149

SOURCE GeneReport for Unigene cluster for RNF149 Gene:


Evidence on tissue expression from TISSUES for RNF149 Gene

  • Nervous system(4.5)
genes like me logo Genes that share expression patterns with RNF149: view

Primer products for research

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for RNF149 Gene

Orthologs for RNF149 Gene

This gene was present in the common ancestor of animals.

Orthologs for RNF149 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia RNF149 30 31
  • 99.25 (n)
(Canis familiaris)
Mammalia RNF149 31
  • 85 (a)
(Bos Taurus)
Mammalia RNF149 30 31
  • 84.31 (n)
(Rattus norvegicus)
Mammalia Rnf149 30
  • 81.55 (n)
(Mus musculus)
Mammalia Rnf149 30 17 31
  • 81.04 (n)
(Monodelphis domestica)
Mammalia RNF149 31
  • 71 (a)
(Ornithorhynchus anatinus)
Mammalia RNF149 31
  • 69 (a)
(Gallus gallus)
Aves RNF149 30 31
  • 76 (n)
(Anolis carolinensis)
Reptilia RNF149 31
  • 61 (a)
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia rnf149 30
  • 66.39 (n)
(Caenorhabditis elegans)
Secernentea H10E21.5 31
  • 21 (a)
Species where no ortholog for RNF149 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Zebrafish (Danio rerio)

Evolution for RNF149 Gene

Gene Tree for RNF149 (if available)
Gene Tree for RNF149 (if available)
Evolutionary constrained regions (ECRs) for RNF149: view image

Paralogs for RNF149 Gene

Paralogs for RNF149 Gene

(7) SIMAP similar genes for RNF149 Gene using alignment to 2 proteins:

  • RN149_HUMAN
genes like me logo Genes that share paralogs with RNF149: view

Variants for RNF149 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for RNF149 Gene

SNP ID Clinical significance and condition Chr 02 pos Variation AA Info Type
rs763258401 A breast cancer sample p.Glu7Lys
rs11123868 - p.Ser9Gly
rs13151 - p.Asp356Glu
rs17856945 - p.Leu344Phe

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for RNF149 Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for RNF149 Gene

Variant ID Type Subtype PubMed ID
esv34123 CNV loss 18971310
esv3591764 CNV loss 21293372
esv3892993 CNV gain 25118596
nsv961845 CNV duplication 23825009

Variation tolerance for RNF149 Gene

Residual Variation Intolerance Score: 68.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 7.85; 83.61% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RNF149 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF149 Gene

Disorders for RNF149 Gene

Additional Disease Information for RNF149

Genetic Association Database
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform

No disorders were found for RNF149 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for RNF149 Gene

Publications for RNF149 Gene

  1. Ring finger protein 149 is an E3 ubiquitin ligase active on wild-type v-Raf murine sarcoma viral oncogene homolog B1 (BRAF). (PMID: 22628551) Hong SW … Kim TW (The Journal of biological chemistry 2012) 3 4
  2. Genome-wide association study of pancreatic cancer in Japanese population. (PMID: 20686608) Low SK … Sakamoto H (PloS one 2010) 3 41
  3. Generation and annotation of the DNA sequences of human chromosomes 2 and 4. (PMID: 15815621) Hillier LW … Wilson RK (Nature 2005) 3 4
  4. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4

Products for RNF149 Gene

Sources for RNF149 Gene