The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. Thi... See more...

Aliases for RNF14 Gene

Aliases for RNF14 Gene

  • Ring Finger Protein 14 2 3 5
  • E3 Ubiquitin-Protein Ligase RNF14 3 4
  • ARA54 3 4
  • HFB30 3 4
  • Androgen Receptor Associated Protein 54 3
  • Androgen Receptor-Associated Protein 54 4
  • RING Finger Protein 14 4
  • Triad2 Protein 4
  • EC 2.3.2.31 4
  • HRIHFB2038 3
  • TRIAD2 3

External Ids for RNF14 Gene

Previous GeneCards Identifiers for RNF14 Gene

  • GC05P141171
  • GC05P141929
  • GC05P141329
  • GC05P141374
  • GC05P141326
  • GC05P136489
  • GC05P141358
  • GC05P141402

Summaries for RNF14 Gene

Entrez Gene Summary for RNF14 Gene

  • The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011]

GeneCards Summary for RNF14 Gene

RNF14 (Ring Finger Protein 14) is a Protein Coding gene. Diseases associated with RNF14 include Prostate Cancer. Among its related pathways are Class I MHC mediated antigen processing and presentation and Innate Immune System. Gene Ontology (GO) annotations related to this gene include ligase activity and androgen receptor binding. An important paralog of this gene is RNF19A.

UniProtKB/Swiss-Prot Summary for RNF14 Gene

  • Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.

Gene Wiki entry for RNF14 Gene

Additional gene information for RNF14 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RNF14 Gene

Genomics for RNF14 Gene

GeneHancer (GH) Regulatory Elements for RNF14 Gene

Promoters and enhancers for RNF14 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RNF14 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RNF14

Top Transcription factor binding sites by QIAGEN in the RNF14 gene promoter:
  • FOXL1
  • NF-kappaB
  • NF-kappaB1
  • RelA

Genomic Locations for RNF14 Gene

Genomic Locations for RNF14 Gene
chr5:141,958,328-141,990,292
(GRCh38/hg38)
Size:
31,965 bases
Orientation:
Plus strand
chr5:141,337,893-141,369,856
(GRCh37/hg19)
Size:
31,964 bases
Orientation:
Plus strand

Genomic View for RNF14 Gene

Genes around RNF14 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RNF14 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RNF14 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF14 Gene

Proteins for RNF14 Gene

  • Protein details for RNF14 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9UBS8-RNF14_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF14
    Protein Accession:
    Q9UBS8
    Secondary Accessions:
    • A0AV26
    • A6NMR2
    • A8MTW5
    • B3KN72
    • B7ZLV2
    • D3DQE4
    • O94793
    • Q6IBV0

    Protein attributes for RNF14 Gene

    Size:
    474 amino acids
    Molecular mass:
    53837 Da
    Quaternary structure:
    • Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2. Interacts with AR/androgen receptor; testosterone- and RNF6-regulated it promotes AR transcriptional activity.

    Alternative splice isoforms for RNF14 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RNF14 Gene

Post-translational modifications for RNF14 Gene

  • RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.
  • Ubiquitination at Lys372 and Lys386
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for RNF14 Gene

Domains & Families for RNF14 Gene

Gene Families for RNF14 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for RNF14 Gene

Blocks:
  • Ubiquitin-conjugating enzymes
  • Zn-finger, RING
  • Zn-finger, cysteine-rich C6HC
  • RWD
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for RNF14 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ58057, highly similar to RING finger protein 14 (B7Z229_HUMAN)
  • cDNA FLJ58064, highly similar to RING finger protein 14 (B7Z3J5_HUMAN)
  • Triad2 protein (RNF14_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9UBS8

UniProtKB/Swiss-Prot:

RNF14_HUMAN :
  • The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
  • Belongs to the RBR family. RNF14 subfamily.
Domain:
  • The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
  • The RING-type zinc finger is essential for the interaction with UBE2E2.
  • Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain.
Family:
  • Belongs to the RBR family. RNF14 subfamily.
genes like me logo Genes that share domains with RNF14: view

Function for RNF14 Gene

Molecular function for RNF14 Gene

UniProtKB/Swiss-Prot Function:
Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.; EC=2.3.2.31; Evidence=. ;.
GENATLAS Biochemistry:
RING finger protein 14,ubiquitously expressed

Enzyme Numbers (IUBMB) for RNF14 Gene

Phenotypes From GWAS Catalog for RNF14 Gene

Gene Ontology (GO) - Molecular Function for RNF14 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003713 transcription coactivator activity TAS 10085091
GO:0004842 ubiquitin-protein transferase activity IEA --
GO:0005515 protein binding IEA,IPI 10085091
GO:0016740 transferase activity IEA --
GO:0019787 ubiquitin-like protein transferase activity IDA 11322894
genes like me logo Genes that share ontologies with RNF14: view
genes like me logo Genes that share phenotypes with RNF14: view

Animal Model Products

CRISPR Products

Clone Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RNF14 Gene

Localization for RNF14 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF14 Gene

Cytoplasm. Nucleus.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RNF14 gene
Compartment Confidence
nucleus 5
cytosol 5
plasma membrane 1
extracellular 1
cytoskeleton 1
mitochondrion 1
endoplasmic reticulum 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for RNF14 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000151 ubiquitin ligase complex IBA 21873635
GO:0005634 nucleus IEA,IDA 11322894
GO:0005737 cytoplasm IBA,IDA 11322894
GO:0005829 cytosol IDA --
genes like me logo Genes that share ontologies with RNF14: view

Pathways & Interactions for RNF14 Gene

genes like me logo Genes that share pathways with RNF14: view

UniProtKB/Swiss-Prot Q9UBS8-RNF14_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for RNF14 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000209 protein polyubiquitination IBA 21873635
GO:0006355 regulation of transcription, DNA-templated IDA 19345326
GO:0006357 regulation of transcription by RNA polymerase II TAS 10085091
GO:0006511 ubiquitin-dependent protein catabolic process IBA 21873635
GO:0007165 signal transduction TAS 10085091
genes like me logo Genes that share ontologies with RNF14: view

No data available for SIGNOR curated interactions for RNF14 Gene

Drugs & Compounds for RNF14 Gene

(2) Drugs for RNF14 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
genes like me logo Genes that share compounds with RNF14: view

Transcripts for RNF14 Gene

mRNA/cDNA for RNF14 Gene

6 REFSEQ mRNAs :
22 NCBI additional mRNA sequence :
16 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for RNF14 Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b · 3c · 3d · 3e ^ 4a · 4b ^ 5 ^ 6a · 6b · 6c · 6d ^ 7a · 7b · 7c ^ 8a · 8b · 8c · 8d · 8e ^ 9a · 9b ^ 10 ^
SP1: - -
SP2:
SP3: - - - - - - - - -
SP4: - - - - - - - - - -
SP5: - - - - -
SP6: - - -
SP7: - - - - - - - - - - - - - -
SP8: - - - - - - - - -
SP9: - - - - - - - -
SP10: - - - - - - -
SP11: - - - - - - - - - -
SP12: - - - - - - - - - - - - -
SP13: - - -
SP14: - - - - - - - - - -
SP15: -
SP16: - - - - - - - - - - - -
SP17: - - - - - - - -
SP18: - - - - - - - -
SP19: - - -
SP20: - - - - - -

ExUns: 11a · 11b · 11c ^ 12a · 12b ^ 13a · 13b · 13c
SP1:
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:
SP15:
SP16:
SP17:
SP18:
SP19:
SP20:

Relevant External Links for RNF14 Gene

GeneLoc Exon Structure for
RNF14

Expression for RNF14 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RNF14 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for RNF14 Gene

This gene is overexpressed in Testis (11.9), Skin (7.1), and Peripheral blood mononuclear cells (6.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for RNF14 Gene



Protein tissue co-expression partners for RNF14 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for RNF14

SOURCE GeneReport for Unigene cluster for RNF14 Gene:

Hs.483616

mRNA Expression by UniProt/SwissProt for RNF14 Gene:

Q9UBS8-RNF14_HUMAN
Tissue specificity: Widely expressed.

Evidence on tissue expression from TISSUES for RNF14 Gene

  • Nervous system(4.8)
  • Thyroid gland(4.3)
genes like me logo Genes that share expression patterns with RNF14: view

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for RNF14 Gene

Orthologs for RNF14 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for RNF14 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RNF14 31 30
  • 99.65 (n)
OneToOne
cow
(Bos Taurus)
Mammalia RNF14 31 30
  • 94.16 (n)
OneToOne
dog
(Canis familiaris)
Mammalia RNF14 31 30
  • 93.74 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Rnf14 30
  • 88.68 (n)
mouse
(Mus musculus)
Mammalia Rnf14 17 31 30
  • 87.97 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia RNF14 31
  • 84 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia RNF14 31
  • 82 (a)
OneToOne
chicken
(Gallus gallus)
Aves RNF14 31 31
  • 77 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia RNF14 31
  • 72 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.3688 30
zebrafish
(Danio rerio)
Actinopterygii rnf14 31
  • 58 (a)
OneToMany
RNF14 (4 of 5) 31
  • 58 (a)
OneToMany
RNF14 (5 of 5) 31
  • 58 (a)
OneToMany
RNF14 (1 of 5) 31
  • 54 (a)
OneToMany
RNF14 (3 of 5) 31
  • 54 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea F56D2.5 31 32
  • 22 (a)
OneToMany
F56D2.2 31 32
  • 18 (a)
OneToMany
Y57A10A.31 31
  • 7 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ITT1 33 31
  • 14 (a)
rice
(Oryza sativa)
Liliopsida Os04g0594000 30
  • 44.19 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 31
  • 41 (a)
OneToOne
Species where no ortholog for RNF14 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for RNF14 Gene

ENSEMBL:
Gene Tree for RNF14 (if available)
TreeFam:
Gene Tree for RNF14 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RNF14: view image

Paralogs for RNF14 Gene

Paralogs for RNF14 Gene

Pseudogenes.org Pseudogenes for RNF14 Gene

genes like me logo Genes that share paralogs with RNF14: view

Variants for RNF14 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for RNF14 Gene

SNP ID Clinical significance and condition Chr 05 pos Variation AA Info Type
791533 Benign: not provided 141,980,347(+) T/G SYNONYMOUS_VARIANT

Additional dbSNP identifiers (rs#s) for RNF14 Gene

Structural Variations from Database of Genomic Variants (DGV) for RNF14 Gene

Variant ID Type Subtype PubMed ID
nsv1154058 CNV deletion 26484159
nsv5036 CNV insertion 18451855
nsv511116 OTHER complex 20534489

Variation tolerance for RNF14 Gene

Residual Variation Intolerance Score: 25.4% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.22; 24.51% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RNF14 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RNF14

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF14 Gene

Disorders for RNF14 Gene

MalaCards: The human disease database

(1) MalaCards diseases for RNF14 Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
prostate cancer
  • prostate cancer, somatic
- elite association - COSMIC cancer census association via MalaCards
Search RNF14 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for RNF14

genes like me logo Genes that share disorders with RNF14: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RNF14 Gene

Publications for RNF14 Gene

  1. Cloning and characterization of human prostate coactivator ARA54, a novel protein that associates with the androgen receptor. (PMID: 10085091) Kang HY … Chang C (The Journal of biological chemistry 1999) 2 3 4 23 54
  2. N-Terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8. (PMID: 11322894) Ito K … Okano Y (European journal of biochemistry 2001) 3 4 23 54
  3. Isolation and characterization of a novel human gene (HFB30) which encodes a protein with a RING finger motif. (PMID: 10320776) Ueki N … Muramatsu M (Biochimica et biophysica acta 1999) 2 3 4 54
  4. Regulation of androgen receptor transcriptional activity and specificity by RNF6-induced ubiquitination. (PMID: 19345326) Xu K … Qiu Y (Cancer cell 2009) 3 4 54
  5. Suppression of androgen receptor transactivation and prostate cancer cell growth by heterogeneous nuclear ribonucleoprotein A1 via interaction with androgen receptor coregulator ARA54. (PMID: 17110431) Yang Z … Chang C (Endocrinology 2007) 3 23 54

Products for RNF14 Gene

  • Biorbyt antibodies for RNF14

Sources for RNF14 Gene