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Aliases for RNF128 Gene

Aliases for RNF128 Gene

  • Ring Finger Protein 128, E3 Ubiquitin Protein Ligase 2 3 5
  • Gene Related To Anergy In Lymphocytes Protein 3 4
  • RING-Type E3 Ubiquitin Transferase RNF128 3 4
  • GRAIL 3 4
  • E3 Ubiquitin-Protein Ligase RNF128 3
  • RING Finger Protein 128 4
  • Ring Finger Protein 128 2
  • EC 2.3.2.27 4

External Ids for RNF128 Gene

Previous GeneCards Identifiers for RNF128 Gene

  • GC0XP103969
  • GC0XP104708
  • GC0XP105743
  • GC0XP105823
  • GC0XP105937
  • GC0XP095565

Summaries for RNF128 Gene

Entrez Gene Summary for RNF128 Gene

  • The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

GeneCards Summary for RNF128 Gene

RNF128 (Ring Finger Protein 128, E3 Ubiquitin Protein Ligase) is a Protein Coding gene. Among its related pathways are Metabolism of proteins and Deubiquitination. Gene Ontology (GO) annotations related to this gene include ligase activity and ubiquitin-protein transferase activity. An important paralog of this gene is RNF133.

UniProtKB/Swiss-Prot for RNF128 Gene

  • E3 ubiquitin-protein ligase that catalyzes Lys-48- and Lys-63-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with Lys-48 linkages and COR1A with Lys-63 linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.

Gene Wiki entry for RNF128 Gene

Additional gene information for RNF128 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RNF128 Gene

Genomics for RNF128 Gene

GeneHancer (GH) Regulatory Elements for RNF128 Gene

Promoters and enhancers for RNF128 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH0XJ106725 Promoter/Enhancer 1.8 EPDnew Ensembl ENCODE 682.7 +35.2 35230 6.5 ELF3 FOXA2 ARID4B DMAP1 ZBTB40 ZNF48 RAD21 RARA YY1 ZNF335 RNF128 CXorf57 TBC1D8B GC0XM106712
GH0XJ106692 Promoter/Enhancer 1.2 EPDnew ENCODE 655.8 +0.2 218 3.2 SOX13 FOXA2 SAP130 ZSCAN4 ZNF384 RAD21 RARA YY1 HNF4G NR2F6 RNF128 FRMPD3 GC0XM106712
GH0XJ106801 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE 14.1 +109.1 109088 1.8 ATF1 ARID4B SIN3A DMAP1 YY1 ETS1 ZNF766 GLIS2 ZNF143 SP3 TBC1D8B RNF128 CLDN2
GH0XJ107672 Promoter/Enhancer 2.3 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 3.8 +982.6 982605 7.7 PKNOX1 CLOCK ARNT SIN3A FEZF1 ZNF2 IRF4 YY1 POLR2B ZNF213 GC0XM107676 NUP62CL TSC22D3 RBM41 MORC4 RNF128 GC0XM107673
GH0XJ107450 Enhancer 1.1 Ensembl ENCODE 7.8 +757.3 757292 1 GLI4 ZNF2 KLF7 ZNF548 SP3 YY2 TSHZ1 ZNF623 GLIS1 MYNN RBM41 DNAJA1P3 MYCLP1 RNF128 FRMPD3 KRT18P49
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RNF128 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the RNF128 gene promoter:
  • USF1
  • USF-1
  • Zic3
  • HOXA9B
  • Meis-1
  • Meis-1b
  • HOXA9
  • Bach1
  • LCR-F1
  • STAT1

Genomic Locations for RNF128 Gene

Genomic Locations for RNF128 Gene
chrX:106,693,794-106,797,016
(GRCh38/hg38)
Size:
103,223 bases
Orientation:
Plus strand
chrX:105,937,024-106,040,246
(GRCh37/hg19)
Size:
103,223 bases
Orientation:
Plus strand

Genomic View for RNF128 Gene

Genes around RNF128 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RNF128 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RNF128 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF128 Gene

Proteins for RNF128 Gene

  • Protein details for RNF128 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8TEB7-RN128_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF128
    Protein Accession:
    Q8TEB7
    Secondary Accessions:
    • A0PJI4
    • Q6PH80
    • Q6ZTJ8
    • Q96RF3
    • Q9H5E4

    Protein attributes for RNF128 Gene

    Size:
    428 amino acids
    Molecular mass:
    46521 Da
    Quaternary structure:
    No Data Available
    SequenceCaution:
    • Sequence=BAB15682.1; Type=Frameshift; Positions=291; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for RNF128 Gene

    Alternative splice isoforms for RNF128 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RNF128 Gene

Post-translational modifications for RNF128 Gene

  • Auto-ubiquitinated. Controls the development of T-cell clonal anergy by ubiquitination.
  • Glycosylation at Asn101, Asn59, and posLast=4848
  • Modification sites at PhosphoSitePlus

Other Protein References for RNF128 Gene

Antibody Products

  • R&D Systems Antibodies for RNF128 (GRAIL/RNF128)
  • Abcam antibodies for RNF128
  • Boster Bio Antibodies for RNF128

No data available for DME Specific Peptides for RNF128 Gene

Domains & Families for RNF128 Gene

Gene Families for RNF128 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted membrane proteins

Protein Domains for RNF128 Gene

Suggested Antigen Peptide Sequences for RNF128 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q8TEB7

UniProtKB/Swiss-Prot:

RN128_HUMAN :
  • Binding to E2 ubiquitin-conjugating enzyme requires an intact RING finger domain.
Domain:
  • Binding to E2 ubiquitin-conjugating enzyme requires an intact RING finger domain.
genes like me logo Genes that share domains with RNF128: view

Function for RNF128 Gene

Molecular function for RNF128 Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase that catalyzes Lys-48- and Lys-63-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with Lys-48 linkages and COR1A with Lys-63 linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
UniProtKB/Swiss-Prot CatalyticActivity:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
UniProtKB/Swiss-Prot Induction:
Induced under anergic conditions. Up-regulated during T-cell anergy induction following signaling through the T-cell antigen receptor.

Enzyme Numbers (IUBMB) for RNF128 Gene

Phenotypes From GWAS Catalog for RNF128 Gene

Gene Ontology (GO) - Molecular Function for RNF128 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 25416956
GO:0016740 transferase activity IEA --
GO:0046872 metal ion binding IEA --
GO:0061630 ubiquitin protein ligase activity TAS,IEA 25635054
genes like me logo Genes that share ontologies with RNF128: view
genes like me logo Genes that share phenotypes with RNF128: view

Animal Models for RNF128 Gene

MGI Knock Outs for RNF128:

Animal Model Products

  • Taconic Biosciences Mouse Models for RNF128

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF128

Clone Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RNF128 Gene

Localization for RNF128 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF128 Gene

Endomembrane system; Single-pass membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Note=Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7A (By similarity). {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RNF128 gene
Compartment Confidence
cytosol 5
cytoskeleton 3
endoplasmic reticulum 3
plasma membrane 2
extracellular 2
golgi apparatus 2
endosome 2
mitochondrion 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for RNF128 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA --
GO:0005770 late endosome IEA --
GO:0005783 endoplasmic reticulum IEA --
GO:0005794 Golgi apparatus IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with RNF128: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for RNF128 Gene

Pathways & Interactions for RNF128 Gene

genes like me logo Genes that share pathways with RNF128: view

UniProtKB/Swiss-Prot Q8TEB7-RN128_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for RNF128 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006511 ubiquitin-dependent protein catabolic process NAS 25635054
GO:0016567 protein ubiquitination IEA --
GO:0016579 protein deubiquitination TAS --
GO:0031647 regulation of protein stability IMP 25635054
GO:0042036 negative regulation of cytokine biosynthetic process IEA --
genes like me logo Genes that share ontologies with RNF128: view

No data available for SIGNOR curated interactions for RNF128 Gene

Drugs & Compounds for RNF128 Gene

(3) Drugs for RNF128 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Adenosine monophosphate Approved, Investigational Nutra 0
ATP Investigational Nutra Agonist, Activator, Full agonist, Antagonist, Potentiation, Pore Blocker 0

(1) Additional Compounds for RNF128 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • [(ho)2P(O)OP(O)(OH)2]
  • Acide diphosphorique
  • Diphosphorsaeure
  • H4P2O7
  • PYROphosphATE
14000-31-8
genes like me logo Genes that share compounds with RNF128: view

Transcripts for RNF128 Gene

mRNA/cDNA for RNF128 Gene

Unigene Clusters for RNF128 Gene

Ring finger protein 128, E3 ubiquitin protein ligase:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF128

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for RNF128 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8 ^ 9
SP1:
SP2: -
SP3: -
SP4:

Relevant External Links for RNF128 Gene

GeneLoc Exon Structure for
RNF128
ECgene alternative splicing isoforms for
RNF128

Expression for RNF128 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for RNF128 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for RNF128 Gene

This gene is overexpressed in Liver (x9.0) and Adrenal Gland (x6.3).

Protein differential expression in normal tissues from HIPED for RNF128 Gene

This gene is overexpressed in Placenta (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for RNF128 Gene



Protein tissue co-expression partners for RNF128 Gene

NURSA nuclear receptor signaling pathways regulating expression of RNF128 Gene:

RNF128

SOURCE GeneReport for Unigene cluster for RNF128 Gene:

Hs.496542

Evidence on tissue expression from TISSUES for RNF128 Gene

  • Nervous system(4.7)
  • Lung(4.2)
genes like me logo Genes that share expression patterns with RNF128: view

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for RNF128 Gene

Orthologs for RNF128 Gene

This gene was present in the common ancestor of animals.

Orthologs for RNF128 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia RNF128 34
  • 96 (a)
OneToOne
dog
(Canis familiaris)
Mammalia RNF128 34 33
  • 93.38 (n)
OneToOne
cow
(Bos Taurus)
Mammalia RNF128 34 33
  • 92.29 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Rnf128 16 34 33
  • 91.51 (n)
rat
(Rattus norvegicus)
Mammalia Rnf128 33
  • 90.73 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia RNF128 34
  • 72 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia RNF128 34
  • 64 (a)
OneToOne
chicken
(Gallus gallus)
Aves RNF128 33
  • 71.97 (n)
-- 34
  • 63 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia -- 34
  • 56 (a)
OneToMany
African clawed frog
(Xenopus laevis)
Amphibia rnf128-A 33
zebrafish
(Danio rerio)
Actinopterygii rnf128a 34 33
  • 59.04 (n)
ManyToMany
si:dkeyp-86f7.4 34
  • 39 (a)
ManyToMany
wufb57h05 33
worm
(Caenorhabditis elegans)
Secernentea H10E21.5 34
  • 18 (a)
OneToMany
Species where no ortholog for RNF128 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)

Evolution for RNF128 Gene

ENSEMBL:
Gene Tree for RNF128 (if available)
TreeFam:
Gene Tree for RNF128 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RNF128: view image

Paralogs for RNF128 Gene

(5) SIMAP similar genes for RNF128 Gene using alignment to 2 proteins:

  • RN128_HUMAN
  • Q5JSK4_HUMAN
genes like me logo Genes that share paralogs with RNF128: view

Variants for RNF128 Gene

Sequence variations from dbSNP and Humsavar for RNF128 Gene

SNP ID Clin Chr 0X pos Variation AA Info Type
rs1000000987 -- 106,752,849(+) G/T intron_variant
rs1000069058 -- 106,778,694(+) C/G intron_variant
rs1000081511 -- 106,708,198(+) C/G genic_upstream_transcript_variant, intron_variant
rs1000098252 -- 106,778,042(+) ACACA/ACA intron_variant
rs1000179292 -- 106,743,753(+) G/T intron_variant

Structural Variations from Database of Genomic Variants (DGV) for RNF128 Gene

Variant ID Type Subtype PubMed ID
esv2667443 CNV deletion 23128226
esv2752312 CNV gain 17911159
nsv1119641 CNV deletion 24896259
nsv510852 CNV deletion 20534489

Variation tolerance for RNF128 Gene

Residual Variation Intolerance Score: 75.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.91; 18.91% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RNF128 Gene

Human Gene Mutation Database (HGMD)
RNF128
SNPedia medical, phenotypic, and genealogical associations of SNPs for
RNF128

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF128 Gene

Disorders for RNF128 Gene

Additional Disease Information for RNF128

No disorders were found for RNF128 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for RNF128 Gene

Publications for RNF128 Gene

  1. GRAIL: an E3 ubiquitin ligase that inhibits cytokine gene transcription is expressed in anergic CD4+ T cells. (PMID: 12705856) Anandasabapathy N … Soares L (Immunity 2003) 3 4 22 58
  2. The DNA sequence of the human X chromosome. (PMID: 15772651) Ross MT … Bentley DR (Nature 2005) 3 4 58
  3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4 58
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 58
  5. E3 ubiquitin ligase RNF128 promotes innate antiviral immunity through K63-linked ubiquitination of TBK1. (PMID: 27776110) Song G … Gao C (Nature immunology 2016) 3 58

Products for RNF128 Gene

Sources for RNF128 Gene

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