The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of ... See more...

Aliases for RNF128 Gene

Aliases for RNF128 Gene

  • Ring Finger Protein 128 2 3 5
  • GRAIL 2 3 4
  • Ring Finger Protein 128, E3 Ubiquitin Protein Ligase 2 3
  • Gene Related To Anergy In Lymphocytes Protein 3 4
  • RING-Type E3 Ubiquitin Transferase RNF128 3 4
  • E3 Ubiquitin-Protein Ligase RNF128 3 4
  • RING Finger Protein 128 4
  • EC 2.3.2.27 4
  • FLJ23516 2
  • EC 6.3.2 51
  • RNF128 5

External Ids for RNF128 Gene

Previous GeneCards Identifiers for RNF128 Gene

  • GC0XP103969
  • GC0XP104708
  • GC0XP105743
  • GC0XP105823
  • GC0XP105937
  • GC0XP095565

Summaries for RNF128 Gene

Entrez Gene Summary for RNF128 Gene

  • The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

GeneCards Summary for RNF128 Gene

RNF128 (Ring Finger Protein 128) is a Protein Coding gene. Among its related pathways are Ovarian tumor domain proteases and Metabolism of proteins. Gene Ontology (GO) annotations related to this gene include ligase activity and ubiquitin-protein transferase activity. An important paralog of this gene is RNF133.

UniProtKB/Swiss-Prot Summary for RNF128 Gene

  • E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.

Gene Wiki entry for RNF128 Gene

Additional gene information for RNF128 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RNF128 Gene

Genomics for RNF128 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for RNF128 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH0XJ106725 Promoter/Enhancer 1.5 EPDnew Ensembl ENCODE 632.8 +35.2 35230 6.5 GATAD2A PRDM10 ZNF692 PRDM1 ZIC2 RBFOX2 POLR2A IRF2 SP1 EZH2 RNF128 RADX TBC1D8B piR-55194-453
GH0XJ106692 Promoter/Enhancer 1.4 EPDnew Ensembl ENCODE 605.7 +0.3 302 3.4 GATAD2A FOXA1 ZSCAN4 RXRB SP1 SOX13 RAD51 FOXA2 YY1 CREM RNF128 FRMPD3 piR-55194-453 TBC1D8B
GH0XJ106801 Promoter/Enhancer 1.8 EPDnew Ensembl ENCODE CraniofacialAtlas 14.1 +109.1 109106 2.6 CREB1 PRDM10 SIX5 ZNF143 PATZ1 ZNF740 SP1 ELF4 GABPA ZNF600 TBC1D8B RNF128 MORC4 RBM41 NONHSAG055066.2 CLDN2
GH0XJ107117 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE CraniofacialAtlas 4.5 +424.5 424485 2.6 PRDM10 RFX1 SIX5 FOXA1 ZNF143 RBFOX2 POLR2A IRF2 ZNF501 GABPA RBM41 FRMPD3 MYCLP1 RNF128 lnc-NUP62CL-2 MORC4
GH0XJ107672 Promoter/Enhancer 2.1 EPDnew FANTOM5 ENCODE CraniofacialAtlas dbSUPER 3.8 +982.6 982605 7.7 CEBPG SP1 CREB1 CTCF PRDM10 ZNF629 RFX1 IKZF1 KDM1A ZNF692 NUP62CL TSC22D3 RBM41 MORC4 RNF128 lnc-PRPS1-3-002 lnc-PRPS1-3-001 lnc-PRPS1-1 PRPS1 NCBP2L
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RNF128 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RNF128

Top Transcription factor binding sites by QIAGEN in the RNF128 gene promoter:
  • Bach1
  • HOXA9
  • HOXA9B
  • LCR-F1
  • Meis-1
  • Meis-1b
  • STAT1
  • USF-1
  • USF1
  • Zic3

Genomic Locations for RNF128 Gene

Genomic Locations for RNF128 Gene
chrX:106,693,794-106,797,016
(GRCh38/hg38)
Size:
103,223 bases
Orientation:
Plus strand
chrX:105,937,024-106,040,246
(GRCh37/hg19)
Size:
103,223 bases
Orientation:
Plus strand

Genomic View for RNF128 Gene

Genes around RNF128 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RNF128 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RNF128 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNF128 Gene

Proteins for RNF128 Gene

  • Protein details for RNF128 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8TEB7-RN128_HUMAN
    Recommended name:
    E3 ubiquitin-protein ligase RNF128
    Protein Accession:
    Q8TEB7
    Secondary Accessions:
    • A0PJI4
    • Q6PH80
    • Q6ZTJ8
    • Q96RF3
    • Q9H5E4

    Protein attributes for RNF128 Gene

    Size:
    428 amino acids
    Molecular mass:
    46521 Da
    Quaternary structure:
    No Data Available
    SequenceCaution:
    • Sequence=BAB15682.1; Type=Frameshift; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for RNF128 Gene

    Alternative splice isoforms for RNF128 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RNF128 Gene

Selected DME Specific Peptides for RNF128 Gene

Q8TEB7:
  • VRILTCNH
  • QVTMVIEVG
  • TAATVGYFIFYSARR
  • NHYSIFFVSVSFFI
  • SETASSGYASV
  • DPWLLEHRTCPMCKCDILK
  • VAIMIGNLKG
  • GPDGDSCAVCIE
  • ALIQRGGGCTF

Post-translational modifications for RNF128 Gene

  • Auto-ubiquitinated. Controls the development of T-cell clonal anergy by ubiquitination.
  • Glycosylation at Asn48, Asn59, and Asn101
  • Modification sites at PhosphoSitePlus

Other Protein References for RNF128 Gene

Domains & Families for RNF128 Gene

Gene Families for RNF128 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted membrane proteins

Protein Domains for RNF128 Gene

InterPro:
Blocks:
  • Zn-finger, RING
  • Protease-associated PA
ProtoNet:

Suggested Antigen Peptide Sequences for RNF128 Gene

GenScript: Design optimal peptide antigens:
  • RNF128 protein (A0PJI4_HUMAN)
  • RING finger protein 128 (RN128_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q8TEB7

UniProtKB/Swiss-Prot:

RN128_HUMAN :
  • Binding to E2 ubiquitin-conjugating enzyme requires an intact RING finger domain.
Domain:
  • Binding to E2 ubiquitin-conjugating enzyme requires an intact RING finger domain.
genes like me logo Genes that share domains with RNF128: view

Function for RNF128 Gene

Molecular function for RNF128 Gene

UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27;.
UniProtKB/Swiss-Prot Induction:
Induced under anergic conditions. Up-regulated during T-cell anergy induction following signaling through the T-cell antigen receptor.

Enzyme Numbers (IUBMB) for RNF128 Gene

Phenotypes From GWAS Catalog for RNF128 Gene

Gene Ontology (GO) - Molecular Function for RNF128 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 25416956
GO:0016740 transferase activity IEA --
GO:0046872 metal ion binding IEA --
GO:0061630 ubiquitin protein ligase activity IEA,TAS 25635054
genes like me logo Genes that share ontologies with RNF128: view
genes like me logo Genes that share phenotypes with RNF128: view

Animal Models for RNF128 Gene

MGI Knock Outs for RNF128:

Animal Model Products

  • Taconic Biosciences Mouse Models for RNF128

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF128

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RNF128 Gene

Localization for RNF128 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNF128 Gene

Endomembrane system. Single-pass membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Note=Localized in an asymmetric perinuclear punctate manner. Localizes to the internal pool of the transferrin recycling endosomal pathway. Partially colocalized with the endoplasmic reticulum resident HSPA5, with Golgi resident STX5, and with the late endosomal GTPase RAB7A (By similarity). {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RNF128 gene
Compartment Confidence
cytosol 4
cytoskeleton 3
endoplasmic reticulum 3
endosome 3
golgi apparatus 3
plasma membrane 2
extracellular 2
mitochondrion 1
nucleus 1
lysosome 1
peroxisome 0

Gene Ontology (GO) - Cellular Components for RNF128 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA,IBA 21873635
GO:0005770 late endosome IEA,IBA 21873635
GO:0005783 endoplasmic reticulum IEA,IBA 21873635
GO:0005794 Golgi apparatus IEA,IBA 21873635
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with RNF128: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for RNF128 Gene

Pathways & Interactions for RNF128 Gene

genes like me logo Genes that share pathways with RNF128: view

UniProtKB/Swiss-Prot Q8TEB7-RN128_HUMAN

  • Pathway: Protein modification; protein ubiquitination.

Gene Ontology (GO) - Biological Process for RNF128 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006511 ubiquitin-dependent protein catabolic process NAS 25635054
GO:0016567 protein ubiquitination IEA --
GO:0016579 protein deubiquitination TAS --
GO:0031647 regulation of protein stability IMP 25635054
GO:0042036 negative regulation of cytokine biosynthetic process IEA --
genes like me logo Genes that share ontologies with RNF128: view

No data available for SIGNOR curated interactions for RNF128 Gene

Drugs & Compounds for RNF128 Gene

(3) Drugs for RNF128 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Adenosine monophosphate Approved, Investigational Nutra 0
ATP Investigational Nutra Agonist, Activator, Partial agonist, Antagonist, Full agonist, Gating inhibitor, Pore Blocker, Potentiation 0

(1) Additional Compounds for RNF128 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • [(ho)2P(O)OP(O)(OH)2]
  • Acide diphosphorique
  • Diphosphorsaeure
  • H4P2O7
  • PYROphosphATE
14000-31-8
genes like me logo Genes that share compounds with RNF128: view

Transcripts for RNF128 Gene

mRNA/cDNA for RNF128 Gene

2 REFSEQ mRNAs :
10 NCBI additional mRNA sequence :
3 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNF128

Alternative Splicing Database (ASD) splice patterns (SP) for RNF128 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8 ^ 9
SP1:
SP2: -
SP3: -
SP4:

Relevant External Links for RNF128 Gene

GeneLoc Exon Structure for
RNF128

Expression for RNF128 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RNF128 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for RNF128 Gene

This gene is overexpressed in Liver (x9.0) and Adrenal Gland (x6.3).

Protein differential expression in normal tissues from HIPED for RNF128 Gene

This gene is overexpressed in Placenta (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for RNF128 Gene



Protein tissue co-expression partners for RNF128 Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for RNF128

SOURCE GeneReport for Unigene cluster for RNF128 Gene:

Hs.496542

Evidence on tissue expression from TISSUES for RNF128 Gene

  • Nervous system(4.8)
  • Lung(4.2)
  • Liver(2.3)
  • Kidney(2.2)
genes like me logo Genes that share expression patterns with RNF128: view

No data available for mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for RNF128 Gene

Orthologs for RNF128 Gene

This gene was present in the common ancestor of animals.

Orthologs for RNF128 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia RNF128 31
  • 96 (a)
OneToOne
Dog
(Canis familiaris)
Mammalia RNF128 30 31
  • 93.38 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia RNF128 30 31
  • 92.29 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Rnf128 30 17 31
  • 91.51 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Rnf128 30
  • 90.73 (n)
Platypus
(Ornithorhynchus anatinus)
Mammalia RNF128 31
  • 72 (a)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia RNF128 31
  • 64 (a)
OneToOne
Chicken
(Gallus gallus)
Aves RNF128 30
  • 71.97 (n)
-- 31
  • 63 (a)
OneToMany
Lizard
(Anolis carolinensis)
Reptilia -- 31
  • 56 (a)
OneToMany
African clawed frog
(Xenopus laevis)
Amphibia rnf128-A 30
Zebrafish
(Danio rerio)
Actinopterygii rnf128a 30 31
  • 59.04 (n)
ManyToMany
si:dkeyp-86f7.4 31
  • 39 (a)
ManyToMany
wufb57h05 30
Worm
(Caenorhabditis elegans)
Secernentea H10E21.5 31
  • 18 (a)
OneToMany
Species where no ortholog for RNF128 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Tropical Clawed Frog (Silurana tropicalis)
  • Wheat (Triticum aestivum)

Evolution for RNF128 Gene

ENSEMBL:
Gene Tree for RNF128 (if available)
TreeFam:
Gene Tree for RNF128 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RNF128: view image

Paralogs for RNF128 Gene

Paralogs for RNF128 Gene

(5) SIMAP similar genes for RNF128 Gene using alignment to 2 proteins:

  • RN128_HUMAN
  • Q5JSK4_HUMAN
genes like me logo Genes that share paralogs with RNF128: view

Variants for RNF128 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for RNF128 Gene

SNP ID Clinical significance and condition Chr 0X pos Variation AA Info Type
667959 Likely Benign: not provided 106,773,059(+) G/A MISSENSE_VARIANT
713571 Benign: not provided 106,727,195(+) C/T SYNONYMOUS_VARIANT,INTRON_VARIANT
787195 Benign: not provided 106,773,038(+) A/T MISSENSE_VARIANT
790294 Likely Benign: not provided 106,791,219(+) C/T MISSENSE_VARIANT
870775 Uncertain Significance: not provided 106,727,295(+) T/G MISSENSE_VARIANT,INTRON_VARIANT

Additional dbSNP identifiers (rs#s) for RNF128 Gene

Structural Variations from Database of Genomic Variants (DGV) for RNF128 Gene

Variant ID Type Subtype PubMed ID
esv2667443 CNV deletion 23128226
esv2752312 CNV gain 17911159
nsv1119641 CNV deletion 24896259
nsv510852 CNV deletion 20534489

Variation tolerance for RNF128 Gene

Residual Variation Intolerance Score: 75.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.91; 18.91% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RNF128 Gene

Human Gene Mutation Database (HGMD)
RNF128
SNPedia medical, phenotypic, and genealogical associations of SNPs for
RNF128

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNF128 Gene

Disorders for RNF128 Gene

No disorders were found for RNF128 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for RNF128 Gene

Publications for RNF128 Gene

  1. GRAIL: an E3 ubiquitin ligase that inhibits cytokine gene transcription is expressed in anergic CD4+ T cells. (PMID: 12705856) Anandasabapathy N … Soares L (Immunity 2003) 3 4 23
  2. The DNA sequence of the human X chromosome. (PMID: 15772651) Ross MT … Bentley DR (Nature 2005) 3 4
  3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4
  5. A reference map of the human binary protein interactome. (PMID: 32296183) Luck K … Calderwood MA (Nature 2020) 3

Products for RNF128 Gene

Sources for RNF128 Gene