The protein encoded by this gene is a member of the ribonuclease A superfamily and functions in the urinary tract. The protein has broad-spectrum antimicrobial activity against pathogenic bacteria. [provided by RefSeq, Nov 2014] See more...

Aliases for RNASE6 Gene

Aliases for RNASE6 Gene

  • Ribonuclease A Family Member K6 2 3 5
  • Ribonuclease, RNase A Family, K6 2 3
  • Ribonuclease K6 3 4
  • RNase K6 2 4
  • RAD1 2 3
  • RNS6 3 4
  • Testicular Tissue Protein Li 166 3
  • Ribonuclease A D1 3
  • EC 3.1.27.5 51
  • EC 3.1.27.- 4
  • EC 3.1.27 51
  • RNasek6 3
  • RNaseK6 2
  • RNASE6 5

External Ids for RNASE6 Gene

Previous HGNC Symbols for RNASE6 Gene

  • RNS6

Previous GeneCards Identifiers for RNASE6 Gene

  • GC14P018621
  • GC14P015036
  • GC14P019239
  • GC14P020319
  • GC14P021249
  • GC14P001370
  • GC14P020781

Summaries for RNASE6 Gene

Entrez Gene Summary for RNASE6 Gene

  • The protein encoded by this gene is a member of the ribonuclease A superfamily and functions in the urinary tract. The protein has broad-spectrum antimicrobial activity against pathogenic bacteria. [provided by RefSeq, Nov 2014]

GeneCards Summary for RNASE6 Gene

RNASE6 (Ribonuclease A Family Member K6) is a Protein Coding gene. Diseases associated with RNASE6 include Nodular Nonsuppurative Panniculitis. Among its related pathways are Innate Immune System and Defensins. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and ribonuclease activity. An important paralog of this gene is RNASE7.

UniProtKB/Swiss-Prot Summary for RNASE6 Gene

  • Ribonuclease which shows a preference for the pyrimidines uridine and cytosine (PubMed:8836175, PubMed:27013146). Has potent antibacterial activity against a range of Gram-positive and Gram-negative bacteria, including P.aeruginosa, A.baumanii, M.luteus, S.aureus, E.faecalis, E.faecium, S.saprophyticus and E.coli (PubMed:25075772, PubMed:27089320). Causes loss of bacterial membrane integrity, and also promotes agglutination of Gram-negative bacteria (PubMed:27089320). Probably contributes to urinary tract sterility (PubMed:25075772). Bactericidal activity is independent of RNase activity (PubMed:27089320).

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RNASE6 Gene

Genomics for RNASE6 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for RNASE6 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH14J020779 Promoter/Enhancer 1.4 EPDnew Ensembl ENCODE 600.7 +1.1 1093 4.2 SP1 BACH1 PKNOX1 TCF12 IKZF2 POLR2A EED ZBTB44 ARID3A ELK1 ENSG00000258810 RNASE6 RNASE1 LOC107984671 EGILA
GH14J020714 Enhancer 1 FANTOM5 Ensembl ENCODE 17.1 -65.5 -65455 5 PRDM10 ZIC2 SCRT2 RBAK OSR2 CTCF ZNF335 SPI1 NFIB FEZF1 RNASE6 ENSG00000258515 METTL3 SUPT16H METTL17 EDDM3B RNASE1 ANG EDDM3A ARHGEF40
GH14J020819 Enhancer 1.2 Ensembl ENCODE dbSUPER 11.1 +38.9 38945 6.6 CTCF ZNF512 LEF1 IKZF1 MTA2 POLR2A DPF2 HDAC1 SOX6 FOXJ2 EDDM3B RNASE6 piR-32813-005 piR-30188-061 RNASE1 RNASE3
GH14J020719 Enhancer 0.6 Ensembl ENCODE 15 -61.0 -61030 1.3 CTBP1 ZNF217 DPF2 SMARCE1 lnc-EDDM3A-1 lnc-OR6S1-4 piR-46682 RNASE6 EDDM3B RNASE1 ANG EDDM3A piR-30188-060 ENSG00000259171
GH14J020711 Enhancer 0.8 Ensembl ENCODE 10.9 -69.2 -69155 0.8 IKZF1 TCF12 EP300 TRIM24 NCOR1 NFIC STAT5A FOXM1 TAL1 DPF2 RNASE1 RNASE6 ANG RANBP20P piR-30188-060 piR-46682 EDDM3A ENSG00000259171 EGILA
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RNASE6 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RNASE6

Top Transcription factor binding sites by QIAGEN in the RNASE6 gene promoter:
  • Brachyury
  • HSF2
  • LCR-F1
  • PPAR-gamma1
  • PPAR-gamma2
  • Zic3

Genomic Locations for RNASE6 Gene

Genomic Locations for RNASE6 Gene
chr14:20,780,956-20,782,467
(GRCh38/hg38)
Size:
1,512 bases
Orientation:
Plus strand
chr14:21,249,210-21,250,626
(GRCh37/hg19)
Size:
1,417 bases
Orientation:
Plus strand

Genomic View for RNASE6 Gene

Genes around RNASE6 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RNASE6 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RNASE6 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RNASE6 Gene

Proteins for RNASE6 Gene

  • Protein details for RNASE6 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q93091-RNAS6_HUMAN
    Recommended name:
    Ribonuclease K6
    Protein Accession:
    Q93091

    Protein attributes for RNASE6 Gene

    Size:
    150 amino acids
    Molecular mass:
    17196 Da
    Quaternary structure:
    • Interacts (via N-terminus) with bacterial lipopolysaccharide (LPS).

    Three dimensional structures from OCA and Proteopedia for RNASE6 Gene

neXtProt entry for RNASE6 Gene

Selected DME Specific Peptides for RNASE6 Gene

Q93091:
  • QNTFLHDSFQ
  • WFEIQHI
  • YPQCRYS
  • LVPVHLD
  • PPQKSDPPY
  • HNCHQSSKPVNMTDCRL
  • NNYTQHCK
  • AHWFEIQHIQPSPLQCNRAM
  • SFQNVAA
  • IQHIQPSPLQ

Post-translational modifications for RNASE6 Gene

  • Glycosylation at Asn55 and Asn100

Other Protein References for RNASE6 Gene

ENSEMBL proteins:
REFSEQ proteins:

Domains & Families for RNASE6 Gene

Gene Families for RNASE6 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted secreted proteins

Protein Domains for RNASE6 Gene

InterPro:
Blocks:
  • Pancreatic ribonuclease
ProtoNet:

Suggested Antigen Peptide Sequences for RNASE6 Gene

GenScript: Design optimal peptide antigens:
  • cDNA, FLJ94144, Homo sapiens ribonuclease, RNase A family, k6 (RNASE6), mRNA (Q6IB39_HUMAN)
  • Ribonuclease K6 (RNAS6_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q93091

UniProtKB/Swiss-Prot:

RNAS6_HUMAN :
  • Belongs to the pancreatic ribonuclease family.
Family:
  • Belongs to the pancreatic ribonuclease family.
genes like me logo Genes that share domains with RNASE6: view

Function for RNASE6 Gene

Molecular function for RNASE6 Gene

UniProtKB/Swiss-Prot Function:
Ribonuclease which shows a preference for the pyrimidines uridine and cytosine (PubMed:8836175, PubMed:27013146). Has potent antibacterial activity against a range of Gram-positive and Gram-negative bacteria, including P.aeruginosa, A.baumanii, M.luteus, S.aureus, E.faecalis, E.faecium, S.saprophyticus and E.coli (PubMed:25075772, PubMed:27089320). Causes loss of bacterial membrane integrity, and also promotes agglutination of Gram-negative bacteria (PubMed:27089320). Probably contributes to urinary tract sterility (PubMed:25075772). Bactericidal activity is independent of RNase activity (PubMed:27089320).
UniProtKB/Swiss-Prot Induction:
Up-regulated in CD14+ monocytes in response to the uropathogenic E.coli strain CFT073.
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=5.0 uM for tRNA {ECO:0000269|PubMed:8836175}; KM=2.63 mM for UpA dinucleotide {ECO:0000269|PubMed:27013146}; KM=1.22 mM for CpA dinucleotide {ECO:0000269|PubMed:27013146}; KM=2.06 mM for cytidine 2,3-cyclic phosphate {ECO:0000269|PubMed:27013146};
GENATLAS Biochemistry:
ribonuclease RNAse A family,k6,ubiquitously expressed

Enzyme Numbers (IUBMB) for RNASE6 Gene

Phenotypes From GWAS Catalog for RNASE6 Gene

Gene Ontology (GO) - Molecular Function for RNASE6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003676 nucleic acid binding IEA --
GO:0004518 nuclease activity IEA --
GO:0004519 endonuclease activity IEA --
GO:0004540 ribonuclease activity IBA,IDA 9826755
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with RNASE6: view
genes like me logo Genes that share phenotypes with RNASE6: view

Animal Model Products

  • Taconic Biosciences Mouse Models for RNASE6

CRISPR Products

miRNA for RNASE6 Gene

miRTarBase miRNAs that target RNASE6

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNASE6

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RNASE6 Gene

Localization for RNASE6 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RNASE6 Gene

Secreted. Lysosome. Cytoplasmic granule.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RNASE6 gene
Compartment Confidence
extracellular 5
lysosome 3
plasma membrane 1
nucleus 1
cytosol 1

Gene Ontology (GO) - Cellular Components for RNASE6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region TAS --
GO:0005615 extracellular space IDA 25075772
GO:0005764 lysosome IEA --
GO:0031410 cytoplasmic vesicle IDA 25075772
genes like me logo Genes that share ontologies with RNASE6: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for RNASE6 Gene

Pathways & Interactions for RNASE6 Gene

PathCards logo

SuperPathways for RNASE6 Gene

genes like me logo Genes that share pathways with RNASE6: view

Pathways by source for RNASE6 Gene

3 Reactome pathways for RNASE6 Gene

Interacting Proteins for RNASE6 Gene

Gene Ontology (GO) - Biological Process for RNASE6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006401 RNA catabolic process TAS 8836175
GO:0006952 defense response TAS 8836175
GO:0019730 antimicrobial humoral response TAS --
GO:0019731 antibacterial humoral response IDA 25075772
GO:0042742 defense response to bacterium IEA --
genes like me logo Genes that share ontologies with RNASE6: view

No data available for SIGNOR curated interactions for RNASE6 Gene

Drugs & Compounds for RNASE6 Gene

(1) Drugs for RNASE6 Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
genes like me logo Genes that share compounds with RNASE6: view

Transcripts for RNASE6 Gene

mRNA/cDNA for RNASE6 Gene

1 REFSEQ mRNAs :
5 NCBI additional mRNA sequence :
1 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RNASE6

Alternative Splicing Database (ASD) splice patterns (SP) for RNASE6 Gene

No ASD Table

Relevant External Links for RNASE6 Gene

GeneLoc Exon Structure for
RNASE6

Expression for RNASE6 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RNASE6 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for RNASE6 Gene

This gene is overexpressed in Whole Blood (x16.0) and Spleen (x4.4).

Protein differential expression in normal tissues from HIPED for RNASE6 Gene

This gene is overexpressed in Kidney (29.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for RNASE6 Gene



Transcriptomic regulation report from SPP (The Signaling Pathways Project) for RNASE6

SOURCE GeneReport for Unigene cluster for RNASE6 Gene:

Hs.23262

mRNA Expression by UniProt/SwissProt for RNASE6 Gene:

Q93091-RNAS6_HUMAN
Tissue specificity: Highly expressed in spleen (at protein level) (PubMed:8836175, PubMed:25075772). Has little or no expression in healthy kidneys (at protein level) (PubMed:25075772). Detected in interstitial leukocytes in infected kidneys (at protein level) (PubMed:25075772). Expressed in ureter where it localizes to urothelial and submucosal leukocytes (at protein level) (PubMed:25075772). Strong expression in lung and thymus, and lower expression in heart, placenta, pancreas, liver, brain and skeletal muscle (PubMed:8836175, PubMed:25075772). Also expressed in monocytes and neutrophils (PubMed:8836175).

Evidence on tissue expression from TISSUES for RNASE6 Gene

  • Spleen(2.4)
  • Lymph node(2.4)
  • Intestine(2.4)
  • Blood(2.3)
  • Nervous system(2.2)
genes like me logo Genes that share expression patterns with RNASE6: view

No data available for Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for RNASE6 Gene

Orthologs for RNASE6 Gene

This gene was present in the common ancestor of chordates.

Orthologs for RNASE6 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia RNASE6 30 31
  • 99.56 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia RNASE6 30 31
  • 79.32 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Rnase6 30 17 31
  • 76.23 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Rnase6 30
  • 75.25 (n)
Dog
(Canis familiaris)
Mammalia RNASE6 31
  • 44 (a)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia -- 31
  • 36 (a)
ManyToMany
Platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 22 (a)
ManyToMany
Chicken
(Gallus gallus)
Aves RSFR 31
  • 24 (a)
ManyToMany
CL2 31
  • 23 (a)
ManyToMany
ANG 31
  • 23 (a)
ManyToMany
Lizard
(Anolis carolinensis)
Reptilia -- 31
  • 26 (a)
ManyToMany
-- 31
  • 23 (a)
ManyToMany
Species where no ortholog for RNASE6 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Tropical Clawed Frog (Silurana tropicalis)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)
  • Zebrafish (Danio rerio)

Evolution for RNASE6 Gene

ENSEMBL:
Gene Tree for RNASE6 (if available)
TreeFam:
Gene Tree for RNASE6 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RNASE6: view image

Paralogs for RNASE6 Gene

(5) SIMAP similar genes for RNASE6 Gene using alignment to 2 proteins:

  • RNAS6_HUMAN
  • Q6IB39_HUMAN
genes like me logo Genes that share paralogs with RNASE6: view

Variants for RNASE6 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for RNASE6 Gene

SNP ID Clinical significance and condition Chr 14 pos Variation AA Info Type
rs1045922 - p.Arg89Gln

Additional dbSNP identifiers (rs#s) for RNASE6 Gene

Structural Variations from Database of Genomic Variants (DGV) for RNASE6 Gene

Variant ID Type Subtype PubMed ID
esv23438 CNV gain+loss 19812545
esv2759972 CNV gain+loss 17122850
nsv428293 CNV gain+loss 18775914

Variation tolerance for RNASE6 Gene

Residual Variation Intolerance Score: 78% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.90; 18.72% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RNASE6 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RNASE6

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RNASE6 Gene

Disorders for RNASE6 Gene

MalaCards: The human disease database

(1) MalaCards diseases for RNASE6 Gene - From: DISEASES

Disorder Aliases PubMed IDs
nodular nonsuppurative panniculitis
  • nodular non-suppurative febrile panniculitis
- elite association - COSMIC cancer census association via MalaCards

Additional Disease Information for RNASE6

genes like me logo Genes that share disorders with RNASE6: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RNASE6 Gene

Publications for RNASE6 Gene

  1. Molecular cloning and characterization of a novel human ribonuclease (RNase k6): increasing diversity in the enlarging ribonuclease gene family. (PMID: 8836175) Rosenberg HF … Dyer KD (Nucleic acids research 1996) 2 3 4 23
  2. Ribonuclease k6: chromosomal mapping and divergent rates of evolution within the RNase A gene superfamily. (PMID: 9647635) Deming MS … Rosenberg HF (Genome research 1998) 2 3 23
  3. Insights into the Antimicrobial Mechanism of Action of Human RNase6: Structural Determinants for Bacterial Cell Agglutination and Membrane Permeation. (PMID: 27089320) Pulido D … Boix E (International journal of molecular sciences 2016) 3 4
  4. Ribonucleases 6 and 7 have antimicrobial function in the human and murine urinary tract. (PMID: 25075772) Becknell B … Spencer JD (Kidney international 2015) 3 4
  5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4

Products for RNASE6 Gene

  • Signalway ELISA kits for RNASE6
  • Signalway Proteins for RNASE6

Sources for RNASE6 Gene