This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011] See more...

Aliases for RHOJ Gene

Aliases for RHOJ Gene

  • Ras Homolog Family Member J 2 3 5
  • TCL 2 3 4
  • Rho-Related GTP-Binding Protein RhoJ 3 4
  • Ras Homolog Gene Family, Member J 2 3
  • Ras-Like Protein Family Member 7B 3 4
  • Tc10-Like GTP-Binding Protein 3 4
  • RAS-Like, Family 7, Member B 2 3
  • RASL7B 3 4
  • ARHJ 3 4
  • TC10-Like Rho GTPase 3
  • FLJ14445 2
  • TC10B 3
  • RHOJ 5
  • RHOI 4

External Ids for RHOJ Gene

Previous HGNC Symbols for RHOJ Gene

  • RASL7B
  • ARHJ

Previous GeneCards Identifiers for RHOJ Gene

  • GC14P061662
  • GC14P062740
  • GC14P063671
  • GC14P043837

Summaries for RHOJ Gene

Entrez Gene Summary for RHOJ Gene

  • This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011]

GeneCards Summary for RHOJ Gene

RHOJ (Ras Homolog Family Member J) is a Protein Coding gene. Diseases associated with RHOJ include Ascaridiasis and Deafness, Autosomal Recessive 104. Among its related pathways are p75 NTR receptor-mediated signalling and Signaling by GPCR. Gene Ontology (GO) annotations related to this gene include GTP binding and GTPase activity. An important paralog of this gene is RHOQ.

UniProtKB/Swiss-Prot Summary for RHOJ Gene

  • Plasma membrane-associated small GTPase specifically involved in angiogenesis (PubMed:21628409, PubMed:24434213, PubMed:30158707). Required for endothelial cell migration during vascular development via its interaction with GLUL (PubMed:30158707). Elicits the formation of F-actin-rich structures, thereby regulating endothelial cell migration (PubMed:30158707).

Gene Wiki entry for RHOJ Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RHOJ Gene

Genomics for RHOJ Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for RHOJ Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH14J063203 Promoter/Enhancer 2.3 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 273.1 +1.0 1021 4.1 IKZF1 JUND ATF2 ZIC2 YY1 HOMEZ FOXA2 SMAD4 SAP130 ETV4 RHOJ KCNH5 GPHB5 LOC105370531
GH14J063181 Enhancer 0.4 Ensembl dbSUPER 22.9 -20.8 -20813 2.2 NR2F2 RHOJ ENSG00000259093 HSALNG0101819 LOC105370531 KCNH5
GH14J063046 Promoter/Enhancer 1.8 EPDnew Ensembl ENCODE CraniofacialAtlas 5.5 -158.7 -158713 2.8 ZNF600 CEBPA ZNF341 FOXA2 RARA SMC3 FOSL2 CTCF REST NRF1 KCNH5 RHOJ piR-43105-168 RF00017-1428
GH14J063174 Enhancer 0.9 Ensembl ENCODE dbSUPER 10.6 -29.3 -29313 2 ZNF654 CTCF RAD21 SMC3 JUND GLIS1 CREB1 EP300 RHOJ ENSG00000259093 LOC105370530 piR-54764-183 LOC105370531 KCNH5
GH14J063214 Enhancer 0.5 Ensembl ENCODE dbSUPER 14.1 +10.7 10687 1.6 RHOJ LOC105370531 HSALNG0101821 GPHB5
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RHOJ on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RHOJ

Top Transcription factor binding sites by QIAGEN in the RHOJ gene promoter:
  • POU2F1
  • POU2F1a
  • POU2F1b
  • POU2F1c
  • POU6F1 (c2)
  • PPAR-gamma1
  • PPAR-gamma2
  • STAT3

Genomic Locations for RHOJ Gene

Latest Assembly
chr14:63,204,114-63,293,508
(GRCh38/hg38)
Size:
89,395 bases
Orientation:
Plus strand

Previous Assembly
chr14:63,671,161-63,760,226
(GRCh37/hg19 by Entrez Gene)
Size:
89,066 bases
Orientation:
Plus strand

chr14:63,670,832-63,759,937
(GRCh37/hg19 by Ensembl)
Size:
89,106 bases
Orientation:
Plus strand

Genomic View for RHOJ Gene

Genes around RHOJ on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RHOJ Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RHOJ Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RHOJ Gene

Proteins for RHOJ Gene

  • Protein details for RHOJ Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9H4E5-RHOJ_HUMAN
    Recommended name:
    Rho-related GTP-binding protein RhoJ
    Protein Accession:
    Q9H4E5
    Secondary Accessions:
    • Q96KC1

    Protein attributes for RHOJ Gene

    Size:
    214 amino acids
    Molecular mass:
    23821 Da
    Quaternary structure:
    • Interacts with the CRIB domains of proteins such as Pak1 and Was/Wasp (By similarity). Interacts with GLUL (PubMed:30158707).
    SequenceCaution:
    • Sequence=BAB55013.1; Type=Frameshift; Evidence={ECO:0000305};
    Miscellaneous:
    • [Isoform 2]: Could be created by usage of an unusual splicing donor site.

    Alternative splice isoforms for RHOJ Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RHOJ Gene

Post-translational modifications for RHOJ Gene

  • Palmitoylated; regulates localization to the plasma membrane and may be mediated by GLUL.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for RHOJ Gene

No data available for DME Specific Peptides for RHOJ Gene

Domains & Families for RHOJ Gene

Gene Families for RHOJ Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for RHOJ Gene

InterPro:
Blocks:
  • Transforming protein P21 RAS signature
  • Ras small GTPase, Rho type

Suggested Antigen Peptide Sequences for RHOJ Gene

GenScript: Design optimal peptide antigens:
  • Tc10-like GTP-binding protein (RHOJ_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9H4E5

UniProtKB/Swiss-Prot:

RHOJ_HUMAN :
  • Belongs to the small GTPase superfamily. Rho family.
Family:
  • Belongs to the small GTPase superfamily. Rho family.
genes like me logo Genes that share domains with RHOJ: view

Function for RHOJ Gene

Molecular function for RHOJ Gene

UniProtKB/Swiss-Prot Function:
Plasma membrane-associated small GTPase specifically involved in angiogenesis (PubMed:21628409, PubMed:24434213, PubMed:30158707). Required for endothelial cell migration during vascular development via its interaction with GLUL (PubMed:30158707). Elicits the formation of F-actin-rich structures, thereby regulating endothelial cell migration (PubMed:30158707).
UniProtKB/Swiss-Prot Induction:
Expression is regulated by the transcription factor ERG.

Phenotypes From GWAS Catalog for RHOJ Gene

Gene Ontology (GO) - Molecular Function for RHOJ Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0003924 GTPase activity IEA,NAS 10967094
GO:0005515 protein binding IPI 25416956
GO:0005525 GTP binding IEA,IDA 27660391
genes like me logo Genes that share ontologies with RHOJ: view
genes like me logo Genes that share phenotypes with RHOJ: view

Animal Models for RHOJ Gene

MGI Knock Outs for RHOJ:
  • Rhoj Rhoj<tm1.2Auem>
  • Rhoj Rhoj<tm1a(KOMP)Wtsi>

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RHOJ

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RHOJ Gene

Localization for RHOJ Gene

Subcellular locations from UniProtKB/Swiss-Prot for RHOJ Gene

Cell membrane. Lipid-anchor. Cytoplasmic side. Note=Localization to the plasma membrane is regulated by GLUL. {ECO:0000269 PubMed:30158707}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RHOJ gene
Compartment Confidence
plasma membrane 5
extracellular 5
cytosol 5
cytoskeleton 3
nucleus 2
endosome 2
lysosome 2
golgi apparatus 2
mitochondrion 1
endoplasmic reticulum 1
peroxisome 0

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoli (2)
  • Nucleoplasm (2)
  • Plasma membrane (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for RHOJ Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane TAS --
GO:0016020 membrane IEA --
GO:0070062 extracellular exosome HDA 23533145
genes like me logo Genes that share ontologies with RHOJ: view

Pathways & Interactions for RHOJ Gene

PathCards logo

SuperPathways for RHOJ Gene

SuperPathway Contained pathways
1 Actin Nucleation by ARP-WASP Complex
.66
.66
2 Salmonella infection (KEGG)
3 Apoptotic Pathways in Synovial Fibroblasts
.62
4 Sertoli-Sertoli Cell Junction Dynamics
.38
5 ERK Signaling
.49
genes like me logo Genes that share pathways with RHOJ: view

Pathways by source for RHOJ Gene

1 BioSystems pathway for RHOJ Gene
1 KEGG pathway for RHOJ Gene
6 Qiagen pathways for RHOJ Gene
  • Actin Nucleation and Branching
  • Actin Nucleation by ARP-WASP Complex
  • Actin-Based Motility by Rho Family GTPases
  • Germ Cell-Sertoli Cell Junction Dynamics
  • ILK Signaling

Gene Ontology (GO) - Biological Process for RHOJ Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001525 angiogenesis IEA --
GO:0006897 endocytosis IBA 21873635
GO:0007264 small GTPase mediated signal transduction IEA --
GO:0007266 Rho protein signal transduction IEA --
GO:0008360 regulation of cell shape IEA --
genes like me logo Genes that share ontologies with RHOJ: view

No data available for SIGNOR curated interactions for RHOJ Gene

Drugs & Compounds for RHOJ Gene

(1) Drugs for RHOJ Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Guanosine triphosphate Experimental Pharma 0
genes like me logo Genes that share compounds with RHOJ: view

Transcripts for RHOJ Gene

mRNA/cDNA for RHOJ Gene

1 REFSEQ mRNAs :
10 NCBI additional mRNA sequence :
4 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RHOJ

Alternative Splicing Database (ASD) splice patterns (SP) for RHOJ Gene

No ASD Table

Relevant External Links for RHOJ Gene

GeneLoc Exon Structure for
RHOJ

Expression for RHOJ Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RHOJ Gene

Protein differential expression in normal tissues from HIPED for RHOJ Gene

This gene is overexpressed in Breast (61.6) and Brain (6.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for RHOJ Gene



Protein tissue co-expression partners for RHOJ Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for RHOJ

SOURCE GeneReport for Unigene cluster for RHOJ Gene:

Hs.656339

mRNA Expression by UniProt/SwissProt for RHOJ Gene:

Q9H4E5-RHOJ_HUMAN
Tissue specificity: Specifically expressed in endothelial cells in different tissues, such as brain, heart, lung and liver.

Evidence on tissue expression from TISSUES for RHOJ Gene

  • Intestine(4.4)
  • Nervous system(3.6)
  • Kidney(2.7)
  • Heart(2.4)
  • Eye(2.4)
  • Muscle(2.3)
  • Skin(2.2)
  • Blood(2.2)
  • Lymph node(2.2)
  • Lung(2.1)
  • Spleen(2.1)
genes like me logo Genes that share expression patterns with RHOJ: view

Primer products for research

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for RHOJ Gene

Orthologs for RHOJ Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RHOJ Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia RHOJ 29 30
  • 99.84 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia RHOJ 29 30
  • 93.15 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia RHOJ 29 30
  • 92.83 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia RHOJ 30
  • 90 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia -- 30
  • 89 (a)
OneToMany
-- 30
  • 84 (a)
OneToMany
Rat
(Rattus norvegicus)
Mammalia Rhoj 29
  • 88.31 (n)
Mouse
(Mus musculus)
Mammalia Rhoj 29 16 30
  • 87.85 (n)
OneToOne
Chicken
(Gallus gallus)
Aves RHOJ 29 30
  • 82.94 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia RHOJ 30
  • 88 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia rhoj 29
  • 76.95 (n)
Str.13316 29
Zebrafish
(Danio rerio)
Actinopterygii rhoj 29 30
  • 74.66 (n)
OneToOne
Fruit Fly
(Drosophila melanogaster)
Insecta RhoL 30
  • 40 (a)
OneToMany
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes CDC42 30
  • 60 (a)
OneToMany
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.10939 30
  • 52 (a)
ManyToMany
CSA.6319 30
  • 50 (a)
ManyToMany
Species where no ortholog for RHOJ was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for RHOJ Gene

ENSEMBL:
Gene Tree for RHOJ (if available)
TreeFam:
Gene Tree for RHOJ (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RHOJ: view image
Alliance of Genome Resources:
Additional Orthologs for RHOJ

Paralogs for RHOJ Gene

(42) SIMAP similar genes for RHOJ Gene using alignment to 3 proteins:

  • RHOJ_HUMAN
  • G3V476_HUMAN
  • G3V4H1_HUMAN
genes like me logo Genes that share paralogs with RHOJ: view

Variants for RHOJ Gene

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for RHOJ Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for RHOJ Gene

Variant ID Type Subtype PubMed ID
dgv137n21 CNV gain 19592680
dgv398e199 CNV deletion 23128226
dgv87e55 CNV gain 17911159
esv2658318 CNV deletion 23128226
esv2748771 CNV deletion 23290073
esv28040 CNV loss 19812545
esv3551642 CNV deletion 23714750
esv3567910 CNV gain 25503493
esv3634722 CNV loss 21293372
esv6302 CNV gain 19470904
nsv1051684 CNV gain 25217958
nsv1153869 CNV deletion 26484159
nsv516899 CNV loss 19592680
nsv521404 CNV loss 19592680
nsv564893 CNV gain 21841781
nsv564894 CNV loss 21841781
nsv564895 CNV loss 21841781
nsv564896 CNV loss 21841781
nsv826963 CNV loss 20364138
nsv832815 CNV gain 17160897
nsv952850 CNV deletion 24416366
nsv957488 CNV deletion 24416366

Variation tolerance for RHOJ Gene

Residual Variation Intolerance Score: 33.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.33; 7.21% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RHOJ Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RHOJ
Leiden Open Variation Database (LOVD)
RHOJ

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for RHOJ Gene

Disorders for RHOJ Gene

MalaCards: The human disease database

(4) MalaCards diseases for RHOJ Gene - From: COP

Disorder Aliases PubMed IDs
ascaridiasis
  • ascariasis
deafness, autosomal recessive 104
  • dfnb104
toxic megacolon
  • megacolon, toxic
cardiomyopathy, familial hypertrophic, 1
  • cmh1
- elite association - COSMIC cancer census association via MalaCards
Search RHOJ in MalaCards View complete list of genes associated with diseases

Additional Disease Information for RHOJ

Human Genome Epidemiology Navigator
(HuGE)
Atlas
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
Open Targets Platform
genes like me logo Genes that share disorders with RHOJ: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RHOJ Gene

Publications for RHOJ Gene

  1. Characterization of TCL, a new GTPase of the rho family related to TC10 andCcdc42. (PMID: 10967094) Vignal E … Fort P (The Journal of biological chemistry 2000) 2 3 4 22
  2. Role of glutamine synthetase in angiogenesis beyond glutamine synthesis. (PMID: 30158707) Eelen G … Carmeliet P (Nature 2018) 3 4
  3. TCL/RhoJ Plasma Membrane Localization and Nucleotide Exchange Is Coordinately Regulated by Amino Acids within the N Terminus and a Distal Loop Region. (PMID: 27660391) Ackermann KL … Hamann MJ (The Journal of biological chemistry 2016) 3 4
  4. Vascular RhoJ is an effective and selective target for tumor angiogenesis and vascular disruption. (PMID: 24434213) Kim C … Koh GY (Cancer cell 2014) 3 4
  5. RhoJ is an endothelial cell-restricted Rho GTPase that mediates vascular morphogenesis and is regulated by the transcription factor ERG. (PMID: 21628409) Yuan L … Oettgen P (Blood 2011) 3 4

Products for RHOJ Gene

Sources for RHOJ Gene