The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential f... See more...

Aliases for RFC3 Gene

Aliases for RFC3 Gene

  • Replication Factor C Subunit 3 2 3 4 5
  • A1 38 KDa Subunit 2 3 4
  • RFC38 2 3 4
  • Replication Factor C (Activator 1) 3, 38kDa 2 3
  • Replication Factor C 38 KDa Subunit 3 4
  • Activator 1 38 KDa Subunit 3 4
  • Activator 1 Subunit 3 3 4
  • RF-C 38 KDa Subunit 3 4
  • RFC, 38 KD Subunit 2 3
  • Replication Factor C (Activator 1) 3 (38kD) 2
  • MGC5276 2
  • RFC3 5

External Ids for RFC3 Gene

Previous GeneCards Identifiers for RFC3 Gene

  • GC13P032378
  • GC13P028378
  • GC13P033328
  • GC13P032190
  • GC13P033290
  • GC13P034392
  • GC13P015203

Summaries for RFC3 Gene

Entrez Gene Summary for RFC3 Gene

  • The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

GeneCards Summary for RFC3 Gene

RFC3 (Replication Factor C Subunit 3) is a Protein Coding gene. Among its related pathways are Homologous DNA Pairing and Strand Exchange and Gastric Cancer Network 2. Gene Ontology (GO) annotations related to this gene include ATPase activity and DNA clamp loader activity. An important paralog of this gene is RFC4.

UniProtKB/Swiss-Prot Summary for RFC3 Gene

  • The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.

Gene Wiki entry for RFC3 Gene

Additional gene information for RFC3 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RFC3 Gene

Genomics for RFC3 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for RFC3 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH13J033817 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 605 +0.5 532 2.8 BRCA1 ARHGAP35 HNRNPL CREB1 GATAD2A ATF7 PRDM10 ZNF629 NFKBIZ ZNF692 RFC3 RF00020-014 RNU5A-4P
GH13J033270 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE CraniofacialAtlas dbSUPER 10.8 -539.5 -539489 16.6 GATAD2A TEAD4 PRDM10 REST ZNF629 ZNF692 RCOR2 FOXA1 PRDM1 RBFOX2 STARD13 N4BP2L2 STARD13-AS RFC3 KL ENSG00000287274 lnc-N4BP2L2-5 piR-55985-038
GH13J033015 Promoter/Enhancer 1.9 EPDnew Ensembl ENCODE CraniofacialAtlas 13.5 -801.4 -801434 3.3 CTCF LARP7 ZNF143 RAD21 IKZF2 TRIM22 EZH2 EED RELB RUNX3 KL lnc-STARD13-4 RFC3 N4BP2L2 STARD13
GH13J033859 Enhancer 0.6 ENCODE 14.4 +41.8 41836 1.5 CREB1 ATF7 ZNF629 SCRT2 ZEB1 ATF2 KLF13 KLF17 CREM ATF3 RFC3 piR-50437-135 piR-46339
GH13J033797 Enhancer 0.5 ENCODE 11.7 -20.1 -20062 0.8 IKZF2 EED RUNX3 JUND IKZF1 BHLHE40 RFC3 ENSG00000286779 lnc-RFC3-4 STARD13
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RFC3 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RFC3

Top Transcription factor binding sites by QIAGEN in the RFC3 gene promoter:
  • AREB6
  • COMP1
  • MyoD
  • MZF-1
  • NF-kappaB
  • NF-kappaB1
  • NF-kappaB2
  • Nkx2-5
  • Roaz
  • Sox9

Genomic Locations for RFC3 Gene

Genomic Locations for RFC3 Gene
chr13:33,818,069-33,973,946
(GRCh38/hg38)
Size:
155,878 bases
Orientation:
Plus strand
chr13:34,392,186-34,540,695
(GRCh37/hg19)
Size:
148,510 bases
Orientation:
Plus strand

Genomic View for RFC3 Gene

Genes around RFC3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RFC3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RFC3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RFC3 Gene

Proteins for RFC3 Gene

  • Protein details for RFC3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P40938-RFC3_HUMAN
    Recommended name:
    Replication factor C subunit 3
    Protein Accession:
    P40938
    Secondary Accessions:
    • C9JU95
    • O15252
    • Q5W0E8

    Protein attributes for RFC3 Gene

    Size:
    356 amino acids
    Molecular mass:
    40556 Da
    Quaternary structure:
    • Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.

    Three dimensional structures from OCA and Proteopedia for RFC3 Gene

    Alternative splice isoforms for RFC3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RFC3 Gene

Post-translational modifications for RFC3 Gene

  • Ubiquitination at Lys20, Lys170, Lys202, and Lys225
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for RFC3 Gene

Domains & Families for RFC3 Gene

Gene Families for RFC3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for RFC3 Gene

Suggested Antigen Peptide Sequences for RFC3 Gene

GenScript: Design optimal peptide antigens:
  • Replication factor C 38 kDa subunit (RFC3_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P40938

UniProtKB/Swiss-Prot:

RFC3_HUMAN :
  • Belongs to the activator 1 small subunits family.
Family:
  • Belongs to the activator 1 small subunits family.
genes like me logo Genes that share domains with RFC3: view

Function for RFC3 Gene

Molecular function for RFC3 Gene

UniProtKB/Swiss-Prot Function:
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
GENATLAS Biochemistry:
replication factor C3,component of complex A-1,DNA polymerase accessory protein "clamp loader",ATP dependent,required to assemble PCNA and polymerase delta on the DNA template

Phenotypes From GWAS Catalog for RFC3 Gene

Gene Ontology (GO) - Molecular Function for RFC3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 contributes_to DNA binding IDA 9488738
GO:0003689 DNA clamp loader activity IBA,TAS 9488738
GO:0005515 protein binding IPI 9488738
GO:0016887 contributes_to ATPase activity IDA 9488738
GO:0017116 contributes_to single-stranded DNA-dependent ATP-dependent DNA helicase activity IDA 12930902
genes like me logo Genes that share ontologies with RFC3: view
genes like me logo Genes that share phenotypes with RFC3: view

Animal Model Products

CRISPR Products

miRNA for RFC3 Gene

miRTarBase miRNAs that target RFC3

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RFC3

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for RFC3 Gene

Localization for RFC3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RFC3 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RFC3 gene
Compartment Confidence
nucleus 5
cytosol 3
extracellular 2
plasma membrane 1
cytoskeleton 1
mitochondrion 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoplasm (3)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for RFC3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IBA 21873635
GO:0005654 nucleoplasm TAS --
GO:0005663 DNA replication factor C complex TAS 7774928
GO:0031390 Ctf18 RFC-like complex IDA 12930902
genes like me logo Genes that share ontologies with RFC3: view

Pathways & Interactions for RFC3 Gene

PathCards logo

SuperPathways for RFC3 Gene

SuperPathway Contained pathways
1 Telomere C-strand (Lagging Strand) Synthesis
.48
2 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template
3 DNA Double-Strand Break Repair
4 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
5 Homologous DNA Pairing and Strand Exchange
genes like me logo Genes that share pathways with RFC3: view

Pathways by source for RFC3 Gene

1 GeneGo (Thomson Reuters) pathway for RFC3 Gene
  • Cell cycle Transition and termination of DNA replication
2 Qiagen pathways for RFC3 Gene
  • Mismatch Repair in Eukaryotes
  • Nucleotide Excision Repair Pathway

Gene Ontology (GO) - Biological Process for RFC3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000731 DNA synthesis involved in DNA repair TAS 9488738
GO:0006260 DNA replication TAS --
GO:0006261 DNA-dependent DNA replication IBA 21873635
GO:0006271 DNA strand elongation involved in DNA replication TAS 7774928
GO:0006281 DNA repair IBA 21873635
genes like me logo Genes that share ontologies with RFC3: view

No data available for SIGNOR curated interactions for RFC3 Gene

Drugs & Compounds for RFC3 Gene

No Compound Related Data Available

Transcripts for RFC3 Gene

mRNA/cDNA for RFC3 Gene

2 REFSEQ mRNAs :
15 NCBI additional mRNA sequence :
3 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RFC3

Alternative Splicing Database (ASD) splice patterns (SP) for RFC3 Gene

No ASD Table

Relevant External Links for RFC3 Gene

GeneLoc Exon Structure for
RFC3

Expression for RFC3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RFC3 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for RFC3 Gene

This gene is overexpressed in Breast (35.7), Bone (11.6), and Lung (8.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for RFC3 Gene



Protein tissue co-expression partners for RFC3 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for RFC3

SOURCE GeneReport for Unigene cluster for RFC3 Gene:

Hs.115474

Evidence on tissue expression from TISSUES for RFC3 Gene

  • Nervous system(3.6)
  • Intestine(2)
genes like me logo Genes that share expression patterns with RFC3: view

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for RFC3 Gene

Orthologs for RFC3 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for RFC3 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia RFC3 30 31
  • 99.25 (n)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia RFC3 31
  • 96 (a)
OneToOne
Cow
(Bos Taurus)
Mammalia RFC3 30 31
  • 94.57 (n)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia RFC3 31
  • 94 (a)
OneToOne
Dog
(Canis familiaris)
Mammalia RFC3 30 31
  • 93.73 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Rfc3 30 17 31
  • 87.83 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Rfc3 30
  • 85.21 (n)
Chicken
(Gallus gallus)
Aves RFC3 30 31
  • 80.71 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia RFC3 31
  • 90 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia rfc3 30
  • 77.25 (n)
Zebrafish
(Danio rerio)
Actinopterygii rfc3 30 31
  • 73.41 (n)
OneToOne
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.6760 30
Fruit Fly
(Drosophila melanogaster)
Insecta BcDNA:LD06837 32
  • 60 (a)
RfC38 30 31
  • 58.74 (n)
OneToOne
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP009047 30
  • 58.69 (n)
Worm
(Caenorhabditis elegans)
Secernentea rfc-3 30 31 32
  • 57.67 (n)
OneToOne
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RFC5 30 31 33
  • 50.39 (n)
OneToOne
A. gosspyii yeast
(Eremothecium gossypii)
Saccharomycetes AGOS_ADL303C 30
  • 49.41 (n)
K. Lactis Yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0B12221g 30
  • 48.94 (n)
Thale Cress
(Arabidopsis thaliana)
eudicotyledons EMB2775 30
  • 56.81 (n)
Soybean
(Glycine max)
eudicotyledons Gma.8866 30
Rice
(Oryza sativa)
Liliopsida Os03g0792600 30
  • 55.94 (n)
Os.28012 30
Barley
(Hordeum vulgare)
Liliopsida Hv.2823 30
Corn
(Zea mays)
Liliopsida Zm.5155 30
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.8435 31
  • 71 (a)
OneToOne
Fission Yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes rfc5 30
  • 56.35 (n)
Bread mold
(Neurospora crassa)
Ascomycetes NCU06769 30
  • 55.33 (n)
Sea Vase
(Ciona intestinalis)
Ascidiacea Cin.8470 30
Species where no ortholog for RFC3 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for RFC3 Gene

ENSEMBL:
Gene Tree for RFC3 (if available)
TreeFam:
Gene Tree for RFC3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RFC3: view image

Paralogs for RFC3 Gene

Paralogs for RFC3 Gene

Pseudogenes.org Pseudogenes for RFC3 Gene

genes like me logo Genes that share paralogs with RFC3: view

Variants for RFC3 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for RFC3 Gene

SNP ID Clinical significance and condition Chr 13 pos Variation AA Info Type
rs3135533 - p.Leu16Val

Additional dbSNP identifiers (rs#s) for RFC3 Gene

Structural Variations from Database of Genomic Variants (DGV) for RFC3 Gene

Variant ID Type Subtype PubMed ID
esv2504202 CNV deletion 19546169
esv2747249 CNV deletion 23290073
esv2747250 CNV deletion 23290073
esv3450965 CNV duplication 20981092
esv3631745 CNV loss 21293372
esv3631746 CNV gain 21293372
nsv1054003 CNV gain 25217958
nsv477781 CNV novel sequence insertion 20440878
nsv527943 CNV loss 19592680
nsv528193 CNV loss 19592680
nsv995 CNV insertion 18451855
nsv996 CNV insertion 18451855

Variation tolerance for RFC3 Gene

Residual Variation Intolerance Score: 32.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.77; 16.30% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RFC3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RFC3

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RFC3 Gene

Disorders for RFC3 Gene

Additional Disease Information for RFC3

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform

No disorders were found for RFC3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for RFC3 Gene

Publications for RFC3 Gene

  1. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (PMID: 20628086) Bailey SD … DREAM investigators (Diabetes care 2010) 3 41
  2. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 41
  3. Polymorphisms in the base excision repair pathway and graft-versus-host disease. (PMID: 20574454) Arora M … Thyagarajan B (Leukemia 2010) 3 41
  4. Association between genetic variants in the base excision repair pathway and outcomes after hematopoietic cell transplantations. (PMID: 20226869) Thyagarajan B … Arora M (Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2010) 3 41
  5. Genome-wide scan of copy number variation in late-onset Alzheimer's disease. (PMID: 20061627) Heinzen EL … Goldstein DB (Journal of Alzheimer's disease : JAD 2010) 3 41

Products for RFC3 Gene

Sources for RFC3 Gene