This gene belongs to the Ras GTPase-activating proteins (RasGAP) family and encodes a protein with pleckstrin homology (PH), C2, and Ras GTPase-activation protein (RasGAP) domains. This protein is localized near or at the plasma membrane when expressed exogenously. Reduced expression of this gene in some cell lines resulted in increased levels of the active form of Ras (Ras-GTP... See more...

Aliases for RASAL3 Gene

Aliases for RASAL3 Gene

  • RAS Protein Activator Like 3 2 3 5
  • RAS Protein Activator Like-3 3 4
  • FLJ21438 2
  • RASAL3 5

External Ids for RASAL3 Gene

Previous GeneCards Identifiers for RASAL3 Gene

  • GC19M015426
  • GC19M015562
  • GC19M015132

Summaries for RASAL3 Gene

Entrez Gene Summary for RASAL3 Gene

  • This gene belongs to the Ras GTPase-activating proteins (RasGAP) family and encodes a protein with pleckstrin homology (PH), C2, and Ras GTPase-activation protein (RasGAP) domains. This protein is localized near or at the plasma membrane when expressed exogenously. Reduced expression of this gene in some cell lines resulted in increased levels of the active form of Ras (Ras-GTP), suggesting that this gene may play a role in negatively regulating the Ras signaling pathway. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

GeneCards Summary for RASAL3 Gene

RASAL3 (RAS Protein Activator Like 3) is a Protein Coding gene. Among its related pathways are RET signaling and Signaling by GPCR. Gene Ontology (GO) annotations related to this gene include GTPase activator activity. An important paralog of this gene is RASAL2.

UniProtKB/Swiss-Prot Summary for RASAL3 Gene

  • Functions as a Ras GTPase-activating protein. Plays an important role in the expansion and functions of natural killer T (NKT) cells in the liver by negatively regulating RAS activity and the down-stream ERK signaling pathway.

Additional gene information for RASAL3 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RASAL3 Gene

Genomics for RASAL3 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for RASAL3 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH19J015463 Promoter/Enhancer 1.1 Ensembl ENCODE 613.8 +0.4 370 2.4 POLR2A LARP7 ZNF143 IKZF2 TRIM22 RELA EED ARID3A ZSCAN29 ELK1 RASAL3 ENSG00000273218 lnc-WIZ-1 WIZ lnc-OR7C2-1
GH19J015466 Promoter/Enhancer 1.3 FANTOM5 Ensembl ENCODE 14.6 -2.4 -2362 1.9 SP1 ZNF512 BACH1 FOXA1 ZNF143 RELA ZNF24 RCOR1 ZNF37A EED RASAL3 RF00017-2861 PGLYRP2 lnc-OR7C2-1
GH19J015479 Promoter/Enhancer 1.3 EPDnew Ensembl ENCODE 13.1 -14.6 -14630 2.4 HNRNPL RXRB CTCF SP1 RFX1 SMARCA5 ESR1 ZNF444 ATF3 ESRRA PGLYRP2 RASAL3 piR-38580-141 MN309174-318 lnc-OR7C2-1
GH19J015295 Promoter/Enhancer 1.5 Ensembl ENCODE dbSUPER 11 +165.9 165861 5.7 CREB1 GATAD2A ATF7 PRDM10 ZNF629 TFE3 SOX13 IKZF1 CTCF ZNF692 AKAP8 ZNF333 BRD4 EPHX3 RASAL3 ENSG00000268189 RNU6-782P CYP4F24P lnc-EPHX3-7 lnc-CYP4F22-6
GH19J015291 Promoter/Enhancer 1.2 Ensembl ENCODE dbSUPER 11 +171.2 171175 4.3 PRDM10 ZNF629 POLR2A ZIC2 ZNF600 SCRT2 CEBPB JUND FOS RBM22 BRD4 AKAP8 AKAP8L EPHX3 RASAL3 RNU6-782P SYDE1 lnc-EPHX3-7 lnc-CYP4F22-6 lnc-OR7C2-1
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RASAL3 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RASAL3

Top Transcription factor binding sites by QIAGEN in the RASAL3 gene promoter:
  • AP-1
  • CP2
  • GR
  • GR-alpha
  • GR-beta
  • Sp1

Genomic Locations for RASAL3 Gene

Genomic Locations for RASAL3 Gene
chr19:15,451,624-15,464,571
(GRCh38/hg38)
Size:
12,948 bases
Orientation:
Minus strand
chr19:15,562,435-15,575,382
(GRCh37/hg19)
Size:
12,948 bases
Orientation:
Minus strand

Genomic View for RASAL3 Gene

Genes around RASAL3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RASAL3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RASAL3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RASAL3 Gene

Proteins for RASAL3 Gene

  • Protein details for RASAL3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q86YV0-RASL3_HUMAN
    Recommended name:
    RAS protein activator like-3
    Protein Accession:
    Q86YV0
    Secondary Accessions:
    • Q8N2T9
    • Q9H735

    Protein attributes for RASAL3 Gene

    Size:
    1011 amino acids
    Molecular mass:
    111898 Da
    Quaternary structure:
    No Data Available
    SequenceCaution:
    • Sequence=AAH30281.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB15064.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAC56928.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=BAC56928.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};

    Alternative splice isoforms for RASAL3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RASAL3 Gene

Post-translational modifications for RASAL3 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for RASAL3 Gene

Domains & Families for RASAL3 Gene

Gene Families for RASAL3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for RASAL3 Gene

InterPro:
Blocks:
  • C2 domain
  • Pleckstrin-like
  • Ras GTPase-activating protein
ProtoNet:

Suggested Antigen Peptide Sequences for RASAL3 Gene

GenScript: Design optimal peptide antigens:
  • RAS protein activator like-3 (RASL3_HUMAN)
genes like me logo Genes that share domains with RASAL3: view

No data available for Graphical View of Domain Structure and UniProtKB/Swiss-Prot for RASAL3 Gene

Function for RASAL3 Gene

Molecular function for RASAL3 Gene

UniProtKB/Swiss-Prot Function:
Functions as a Ras GTPase-activating protein. Plays an important role in the expansion and functions of natural killer T (NKT) cells in the liver by negatively regulating RAS activity and the down-stream ERK signaling pathway.

Phenotypes From GWAS Catalog for RASAL3 Gene

Gene Ontology (GO) - Molecular Function for RASAL3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005096 GTPase activator activity IEA --
genes like me logo Genes that share ontologies with RASAL3: view
genes like me logo Genes that share phenotypes with RASAL3: view

Animal Models for RASAL3 Gene

MGI Knock Outs for RASAL3:

Animal Model Products

CRISPR Products

miRNA for RASAL3 Gene

miRTarBase miRNAs that target RASAL3

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RASAL3

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RASAL3 Gene

Localization for RASAL3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RASAL3 Gene

Cytoplasm. Cytoplasm, cell cortex.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RASAL3 gene
Compartment Confidence
cytosol 5
extracellular 4
nucleus 2
plasma membrane 1
lysosome 1
mitochondrion 0
peroxisome 0

Subcellular locations from the

Human Protein Atlas (HPA)
  • Plasma membrane (3)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for RASAL3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA,IDA 25652366
GO:0005829 cytosol TAS --
GO:0005938 cell cortex IEA --
GO:0016020 membrane HDA 19946888
GO:0070062 extracellular exosome HDA 20458337
genes like me logo Genes that share ontologies with RASAL3: view

Pathways & Interactions for RASAL3 Gene

PathCards logo

SuperPathways for RASAL3 Gene

SuperPathway Contained pathways
1 RET signaling
2 Developmental Biology
3 Cytokine Signaling in Immune system
4 HIV Life Cycle
.45
5 Innate Immune System
genes like me logo Genes that share pathways with RASAL3: view

Pathways by source for RASAL3 Gene

Gene Ontology (GO) - Biological Process for RASAL3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000165 MAPK cascade TAS --
GO:0007165 signal transduction IEA --
GO:0043087 regulation of GTPase activity IEA --
GO:0043547 positive regulation of GTPase activity IEA --
GO:0046580 negative regulation of Ras protein signal transduction IMP 25652366
genes like me logo Genes that share ontologies with RASAL3: view

No data available for SIGNOR curated interactions for RASAL3 Gene

Drugs & Compounds for RASAL3 Gene

No Compound Related Data Available

Transcripts for RASAL3 Gene

mRNA/cDNA for RASAL3 Gene

3 REFSEQ mRNAs :
8 NCBI additional mRNA sequence :
7 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RASAL3

Alternative Splicing Database (ASD) splice patterns (SP) for RASAL3 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9a · 9b ^ 10 ^ 11 ^ 12 ^ 13a · 13b ^ 14 ^ 15 ^ 16 ^ 17a · 17b ^ 18a · 18b ^ 19a · 19b
SP1:
SP2:
SP3: - -
SP4:
SP5: - - -
SP6:
SP7:
SP8:
SP9: -
SP10:

Relevant External Links for RASAL3 Gene

GeneLoc Exon Structure for
RASAL3

Expression for RASAL3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RASAL3 Gene

mRNA differential expression in normal tissues according to GTEx for RASAL3 Gene

This gene is overexpressed in Whole Blood (x17.0), Spleen (x7.6), and Small Intestine - Terminal Ileum (x4.6).

Protein differential expression in normal tissues from HIPED for RASAL3 Gene

This gene is overexpressed in Tlymphocyte (26.0), Peripheral blood mononuclear cells (19.3), NK cells (8.6), and CD8 Tcells (6.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for RASAL3 Gene



Protein tissue co-expression partners for RASAL3 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for RASAL3

SOURCE GeneReport for Unigene cluster for RASAL3 Gene:

Hs.136979

mRNA Expression by UniProt/SwissProt for RASAL3 Gene:

Q86YV0-RASL3_HUMAN
Tissue specificity: Predominantly expressed in cells of hematopoietic lineages.

Evidence on tissue expression from TISSUES for RASAL3 Gene

  • Spleen(4.4)
  • Blood(4.3)
  • Intestine(4.3)
  • Liver(4.2)
  • Lymph node(2.2)
genes like me logo Genes that share expression patterns with RASAL3: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and Phenotype-based relationships between genes and organs from Gene ORGANizer for RASAL3 Gene

Orthologs for RASAL3 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RASAL3 Gene

Organism Taxonomy Gene Similarity Type Details
Dog
(Canis familiaris)
Mammalia RASAL3 30 31
  • 85.87 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia RASAL3 30 31
  • 83.75 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Rasal3 30 17 31
  • 80.17 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Rasal3 30
  • 79.74 (n)
Oppossum
(Monodelphis domestica)
Mammalia RASAL3 31
  • 65 (a)
OneToOne
Platypus
(Ornithorhynchus anatinus)
Mammalia RASAL3 31
  • 59 (a)
OneToOne
Chicken
(Gallus gallus)
Aves RASAL3 31
  • 43 (a)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia -- 31
  • 30 (a)
ManyToMany
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia rasal3 30
  • 50.33 (n)
Zebrafish
(Danio rerio)
Actinopterygii rasal3 31
  • 31 (a)
OneToOne
Fruit Fly
(Drosophila melanogaster)
Insecta CG42684 31
  • 14 (a)
OneToMany
Worm
(Caenorhabditis elegans)
Secernentea gap-2 31
  • 20 (a)
OneToMany
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes BUD2 31
  • 14 (a)
OneToMany
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.6146 31
  • 23 (a)
OneToMany
Species where no ortholog for RASAL3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chimpanzee (Pan troglodytes)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for RASAL3 Gene

ENSEMBL:
Gene Tree for RASAL3 (if available)
TreeFam:
Gene Tree for RASAL3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RASAL3: view image

Paralogs for RASAL3 Gene

(4) SIMAP similar genes for RASAL3 Gene using alignment to 3 proteins:

  • RASL3_HUMAN
  • M0QX12_HUMAN
  • V9GYD1_HUMAN
genes like me logo Genes that share paralogs with RASAL3: view

Variants for RASAL3 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for RASAL3 Gene

SNP ID Clinical significance and condition Chr 19 pos Variation AA Info Type
rs76267899 Uncertain Significance: Acute megakaryoblastic leukemia; Mediastinal germ cell tumor 15,464,363(-) G/C FIVE_PRIME_UTR_VARIANT
rs56209154 - p.Arg594Cys
rs57208996 - p.Pro825Thr
rs58123634 - p.Leu251Val

Additional dbSNP identifiers (rs#s) for RASAL3 Gene

Structural Variations from Database of Genomic Variants (DGV) for RASAL3 Gene

Variant ID Type Subtype PubMed ID
esv2551856 CNV deletion 19546169
nsv1064684 CNV gain 25217958
nsv1118816 CNV deletion 24896259
nsv1131404 CNV deletion 24896259
nsv482216 CNV loss 20164927
nsv833765 CNV loss 17160897

Variation tolerance for RASAL3 Gene

Residual Variation Intolerance Score: 69.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 5.55; 72.17% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RASAL3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RASAL3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RASAL3 Gene

Disorders for RASAL3 Gene

No disorders were found for RASAL3 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for RASAL3 Gene

Publications for RASAL3 Gene

  1. RASAL3, a novel hematopoietic RasGAP protein, regulates the number and functions of NKT cells. (PMID: 25652366) Saito S … Fujii M (European journal of immunology 2015) 2 3 4
  2. RASAL3 preferentially stimulates GTP hydrolysis of the Rho family small GTPase Rac2. (PMID: 30271600) Shin Y … Chang JS (Biomedical reports 2018) 2 3
  3. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4
  4. A reference map of the human binary protein interactome. (PMID: 32296183) Luck K … Calderwood MA (Nature 2020) 3
  5. N-terminome analysis of the human mitochondrial proteome. (PMID: 25944712) Vaca Jacome AS … Carapito C (Proteomics 2015) 4

Products for RASAL3 Gene

Sources for RASAL3 Gene