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Aliases for RAD9A Gene

Aliases for RAD9A Gene

  • RAD9 Checkpoint Clamp Component A 2 3 5
  • DNA Repair Exonuclease Rad9 Homolog A 3 4
  • HRAD9 3 4
  • Cell Cycle Checkpoint Control Protein RAD9A 3
  • RAD9 Homolog A (S. Pombe) 2
  • RAD9 (S. Pombe) Homolog 2
  • RAD9 Homolog A 3
  • EC 3.1.11.2 4
  • RAD9 3

External Ids for RAD9A Gene

Previous HGNC Symbols for RAD9A Gene

  • RAD9

Previous GeneCards Identifiers for RAD9A Gene

  • GC11P067409
  • GC11P066934
  • GC11P066915
  • GC11P067159
  • GC11P063487

Summaries for RAD9A Gene

Entrez Gene Summary for RAD9A Gene

  • This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

GeneCards Summary for RAD9A Gene

RAD9A (RAD9 Checkpoint Clamp Component A) is a Protein Coding gene. Diseases associated with RAD9A include Alpha-Thalassemia/Mental Retardation Syndrome, X-Linked. Among its related pathways are Gene Expression and Cell Cycle, Mitotic. Gene Ontology (GO) annotations related to this gene include protein kinase binding and SH3 domain binding. An important paralog of this gene is RAD9B.

UniProtKB/Swiss-Prot for RAD9A Gene

  • Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3->5 double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.

Gene Wiki entry for RAD9A Gene

Additional gene information for RAD9A Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for RAD9A Gene

Genomics for RAD9A Gene

GeneHancer (GH) Regulatory Elements for RAD9A Gene

Promoters and enhancers for RAD9A Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH11J067390 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 672.6 +74.0 74007 2.2 CLOCK MLX ARNT ZFP64 ARID4B SIN3A FEZF1 DMAP1 YY1 E2F8 RAD9A PIR46028 RBM14 KMT5B ENSG00000258297 RBM4 RCE1 MRPL11 KDM2A RHOD
GH11J067316 Promoter/Enhancer 1.6 Ensembl ENCODE 650.7 -0.3 -300 2.3 HDGF SMAD1 ARID4B SIN3A DMAP1 ZNF2 YY1 SLC30A9 POLR2B ZNF213 RAD9A LOC100130987 SSH3 MRPL11 ENSG00000258297 RBM4 KMT5B RBM14 GRK2 UNC93B1
GH11J067116 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE dbSUPER 42.4 -198.2 -198168 5.7 PKNOX1 ARID4B SIN3A DMAP1 IRF4 YY1 POLR2B ZNF766 ZNF207 SP3 KDM2A ENSG00000258297 RBM14 ENSG00000254461 LOC105369352 RBM4 RAD9A RCE1 ENSG00000245156 BBS1
GH11J066342 Promoter/Enhancer 2.6 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 24.2 -969.3 -969326 11.8 MLX ZFP64 DMAP1 IRF4 YY1 SLC30A9 ZNF213 E2F8 ZNF143 SP3 LOC102724064 B4GAT1 TRS-GCT3-1 RBM14 ENSG00000258297 LOC105369352 RBM4 EIF1AD RIN1 ENSG00000255320
GH11J068098 Enhancer 1.4 Ensembl ENCODE dbSUPER 42.4 +783.2 783171 4.7 FOXA2 SMAD1 MLX ZFP64 ARID4B DMAP1 YY1 SLC30A9 ZNF213 SP5 KMT5B PPP6R3 MRGPRF-AS1 ENSG00000260808 ENSG00000261625 TPCN2 RAD9A MRGPRF KDM2A CORO1B
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RAD9A on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the RAD9A gene promoter:
  • STAT3
  • Sp1
  • p53

Genomic Locations for RAD9A Gene

Genomic Locations for RAD9A Gene
chr11:67,317,871-67,398,412
(GRCh38/hg38)
Size:
80,542 bases
Orientation:
Plus strand
chr11:67,159,176-67,165,883
(GRCh37/hg19)
Size:
6,708 bases
Orientation:
Plus strand

Genomic View for RAD9A Gene

Genes around RAD9A on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RAD9A Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RAD9A Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RAD9A Gene

Proteins for RAD9A Gene

  • Protein details for RAD9A Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q99638-RAD9A_HUMAN
    Recommended name:
    Cell cycle checkpoint control protein RAD9A
    Protein Accession:
    Q99638
    Secondary Accessions:
    • B2RCZ8
    • Q6FI29
    • Q96C41

    Protein attributes for RAD9A Gene

    Size:
    391 amino acids
    Molecular mass:
    42547 Da
    Quaternary structure:
    • Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD9A interacts with BCL2L1, FEN1, PRKCD, RAD9B, HUS1, RAD1, ABL1, RPA1, ATAD5 and RPA2. Interacts with DNAJC7 and RHNO1.

    Three dimensional structures from OCA and Proteopedia for RAD9A Gene

neXtProt entry for RAD9A Gene

Post-translational modifications for RAD9A Gene

  • Constitutively phosphorylated on serine and threonine amino acids in absence of DNA damage. Hyperphosphorylated by PRKCD and ABL1 upon DNA damage. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for RAD9A Gene

Domains & Families for RAD9A Gene

Gene Families for RAD9A Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins

Protein Domains for RAD9A Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for RAD9A Gene

Graphical View of Domain Structure for InterPro Entry

Q99638

UniProtKB/Swiss-Prot:

RAD9A_HUMAN :
  • Belongs to the rad9 family.
Family:
  • Belongs to the rad9 family.
genes like me logo Genes that share domains with RAD9A: view

Function for RAD9A Gene

Molecular function for RAD9A Gene

UniProtKB/Swiss-Prot Function:
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3->5 double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
UniProtKB/Swiss-Prot CatalyticActivity:
Exonucleolytic cleavage in the 3- to 5-direction to yield nucleoside 5-phosphates.

Enzyme Numbers (IUBMB) for RAD9A Gene

Phenotypes From GWAS Catalog for RAD9A Gene

Gene Ontology (GO) - Molecular Function for RAD9A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004518 nuclease activity IEA --
GO:0004527 exonuclease activity IEA --
GO:0005515 protein binding IPI 10359610
GO:0008408 3-5 exonuclease activity IDA 10713044
GO:0008853 exodeoxyribonuclease III activity IEA --
genes like me logo Genes that share ontologies with RAD9A: view
genes like me logo Genes that share phenotypes with RAD9A: view

Animal Models for RAD9A Gene

MGI Knock Outs for RAD9A:
  • Rad9a Rad9a<tm1.1(KOMP)Wtsi>
  • Rad9a Rad9a<tm1Lieb>

Animal Model Products

miRNA for RAD9A Gene

miRTarBase miRNAs that target RAD9A

Clone Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RAD9A Gene

Localization for RAD9A Gene

Subcellular locations from UniProtKB/Swiss-Prot for RAD9A Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RAD9A gene
Compartment Confidence
nucleus 5
cytosol 2
mitochondrion 1
peroxisome 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for RAD9A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA,IEA 12628935
GO:0005654 nucleoplasm TAS,IDA --
GO:0005737 cytoplasm IEA --
GO:0030896 checkpoint clamp complex IEA,IBA --
genes like me logo Genes that share ontologies with RAD9A: view

Pathways & Interactions for RAD9A Gene

genes like me logo Genes that share pathways with RAD9A: view

SIGNOR curated interactions for RAD9A Gene

Activates:
Is activated by:
Other effect:

Gene Ontology (GO) - Biological Process for RAD9A Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000076 DNA replication checkpoint TAS 8943031
GO:0000077 DNA damage checkpoint IMP,IEA 21659603
GO:0006260 DNA replication TAS --
GO:0006281 DNA repair IBA --
GO:0006974 cellular response to DNA damage stimulus IEA,IDA 9872989
genes like me logo Genes that share ontologies with RAD9A: view

Drugs & Compounds for RAD9A Gene

(1) Drugs for RAD9A Gene - From: Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
genes like me logo Genes that share compounds with RAD9A: view

Transcripts for RAD9A Gene

Unigene Clusters for RAD9A Gene

RAD9 homolog A (S. pombe):
Representative Sequences:

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for RAD9A Gene

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10
SP1:
SP2: -
SP3:
SP4:

Relevant External Links for RAD9A Gene

GeneLoc Exon Structure for
RAD9A
ECgene alternative splicing isoforms for
RAD9A

Expression for RAD9A Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for RAD9A Gene

Protein differential expression in normal tissues from HIPED for RAD9A Gene

This gene is overexpressed in Peripheral blood mononuclear cells (46.9).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for RAD9A Gene



Protein tissue co-expression partners for RAD9A Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of RAD9A Gene:

RAD9A

SOURCE GeneReport for Unigene cluster for RAD9A Gene:

Hs.655354

Evidence on tissue expression from TISSUES for RAD9A Gene

  • Liver(4.2)
genes like me logo Genes that share expression patterns with RAD9A: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for RAD9A Gene

Orthologs for RAD9A Gene

This gene was present in the common ancestor of animals.

Orthologs for RAD9A Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia RAD9A 34 33
  • 90.86 (n)
OneToOne
dog
(Canis familiaris)
Mammalia RAD9A 34 33
  • 87.44 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Rad9a 16 34 33
  • 84.49 (n)
rat
(Rattus norvegicus)
Mammalia Rad9a 33
  • 84.4 (n)
oppossum
(Monodelphis domestica)
Mammalia RAD9A 34
  • 74 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia RAD9A 34
  • 57 (a)
OneToOne
chicken
(Gallus gallus)
Aves RAD9A 33
  • 66.3 (n)
RAD9 34
  • 55 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia RAD9A 34
  • 57 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia rad9a 33
  • 58.06 (n)
African clawed frog
(Xenopus laevis)
Amphibia Rad9 33
zebrafish
(Danio rerio)
Actinopterygii rad9a 34
  • 50 (a)
OneToOne
zgc56197 33
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.520 33
fruit fly
(Drosophila melanogaster)
Insecta Rad9 34 33
  • 47.62 (n)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea hpr-9 34
  • 14 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 28 (a)
OneToMany
Species where no ortholog for RAD9A was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chimpanzee (Pan troglodytes)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RAD9A Gene

ENSEMBL:
Gene Tree for RAD9A (if available)
TreeFam:
Gene Tree for RAD9A (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RAD9A: view image

Paralogs for RAD9A Gene

Paralogs for RAD9A Gene

(1) SIMAP similar genes for RAD9A Gene using alignment to 6 proteins:

  • RAD9A_HUMAN
  • C4TNW7_HUMAN
  • C4TNW8_HUMAN
  • F5H492_HUMAN
  • F5H4F1_HUMAN
  • F5H8A2_HUMAN
genes like me logo Genes that share paralogs with RAD9A: view

Variants for RAD9A Gene

Sequence variations from dbSNP and Humsavar for RAD9A Gene

SNP ID Clin Chr 11 pos Variation AA Info Type
rs1000263849 -- 67,392,430(+) C/T 5_prime_UTR_variant, genic_upstream_transcript_variant, intron_variant, non_coding_transcript_variant, upstream_transcript_variant
rs1000361449 -- 67,391,406(+) TT/TTT upstream_transcript_variant
rs1000420135 -- 67,397,913(+) G/A 3_prime_UTR_variant, non_coding_transcript_variant
rs1001059730 -- 67,396,963(+) C/A intron_variant
rs1001347598 -- 67,397,088(+) G/A intron_variant

Structural Variations from Database of Genomic Variants (DGV) for RAD9A Gene

Variant ID Type Subtype PubMed ID
dgv1977n54 CNV loss 21841781
dgv52n111 CNV deletion 26073780
esv1010387 CNV loss 20482838
nsv468603 CNV loss 19166990
nsv516453 CNV loss 19592680
nsv555253 CNV loss 21841781

Variation tolerance for RAD9A Gene

Residual Variation Intolerance Score: 69.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.58; 44.74% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RAD9A Gene

Human Gene Mutation Database (HGMD)
RAD9A
SNPedia medical, phenotypic, and genealogical associations of SNPs for
RAD9A

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RAD9A Gene

Disorders for RAD9A Gene

MalaCards: The human disease database

(1) MalaCards diseases for RAD9A Gene - From: HGMD and GeneCards

- elite association - COSMIC cancer census association via MalaCards
Search RAD9A in MalaCards View complete list of genes associated with diseases

Additional Disease Information for RAD9A

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with RAD9A: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RAD9A Gene

Publications for RAD9A Gene

  1. Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells. (PMID: 15897895) Wu X … Zou Y (Oncogene 2005) 3 4 22 58
  2. Protein kinase Cdelta is responsible for constitutive and DNA damage-induced phosphorylation of Rad9. (PMID: 12628935) Yoshida K … Kufe D (The EMBO journal 2003) 3 4 22 58
  3. Phosphorylation of human Rad9 is required for genotoxin-activated checkpoint signaling. (PMID: 12709442) Roos-Mattjus P … Karnitz LM (The Journal of biological chemistry 2003) 3 4 22 58
  4. Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue. (PMID: 14500360) Hopkins KM … Lieberman HB (Cancer research 2003) 3 4 22 58
  5. c-Abl tyrosine kinase regulates the human Rad9 checkpoint protein in response to DNA damage. (PMID: 11971963) Yoshida K … Kufe D (Molecular and cellular biology 2002) 3 4 22 58

Products for RAD9A Gene

Sources for RAD9A Gene

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