The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombinatio... See more...

Aliases for RAD52 Gene

Aliases for RAD52 Gene

  • RAD52 Homolog, DNA Repair Protein 2 3 5
  • DNA Repair Protein RAD52 Homolog 3 4
  • Rhabdomyosarcoma Antigen MU-RMS-40.23 3
  • RAD52 (S. Cerevisiae) Homolog 2
  • RAD52 Homolog (S. Cerevisiae) 2
  • Recombination Protein RAD52 3
  • RAD52 5

External Ids for RAD52 Gene

Previous GeneCards Identifiers for RAD52 Gene

  • GC12M000841
  • GC12M000891
  • GC12M001021

Summaries for RAD52 Gene

Entrez Gene Summary for RAD52 Gene

  • The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

GeneCards Summary for RAD52 Gene

RAD52 (RAD52 Homolog, DNA Repair Protein) is a Protein Coding gene. Diseases associated with RAD52 include Werner Syndrome and Progressive Myoclonus Epilepsy 8. Among its related pathways are Metabolism of proteins and DNA Damage Response. Gene Ontology (GO) annotations related to this gene include identical protein binding and recombinase activity.

UniProtKB/Swiss-Prot Summary for RAD52 Gene

  • Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.

Gene Wiki entry for RAD52 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RAD52 Gene

Genomics for RAD52 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for RAD52 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RAD52 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for RAD52

Top Transcription factor binding sites by QIAGEN in the RAD52 gene promoter:
  • GATA-1
  • GATA-2
  • GATA-3
  • p53
  • ZIC2

Genomic Locations for RAD52 Gene

Genomic Locations for RAD52 Gene
chr12:911,028-991,195
(GRCh38/hg38)
Size:
80,168 bases
Orientation:
Minus strand
chr12:1,021,243-1,099,219
(GRCh37/hg19)
Size:
77,977 bases
Orientation:
Minus strand

Genomic View for RAD52 Gene

Genes around RAD52 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RAD52 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RAD52 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for RAD52 Gene

Proteins for RAD52 Gene

  • Protein details for RAD52 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P43351-RAD52_HUMAN
    Recommended name:
    DNA repair protein RAD52 homolog
    Protein Accession:
    P43351
    Secondary Accessions:
    • Q13205
    • Q9Y5T7
    • Q9Y5T8
    • Q9Y5T9

    Protein attributes for RAD52 Gene

    Size:
    418 amino acids
    Molecular mass:
    46169 Da
    Quaternary structure:
    • The full-length protein forms heptameric rings. Interacts with ABL1. Interacts with RPA2; the interaction is direct and associates RAD52 with the RPA complex.
    Miscellaneous:
    • [Isoform beta]: Unable to interact with isoform alpha, may act as dominant negative.
    • [Isoform gamma]: Unable to interact with isoform alpha, may act as dominant negative.
    • [Isoform delta]: Unable to interact with isoform alpha, may act as dominant negative.

    Three dimensional structures from OCA and Proteopedia for RAD52 Gene

    Alternative splice isoforms for RAD52 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RAD52 Gene

Post-translational modifications for RAD52 Gene

  • Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for RAD52 Gene

Domains & Families for RAD52 Gene

Gene Families for RAD52 Gene

Human Protein Atlas (HPA):
  • Cancer-related genes
  • Predicted intracellular proteins

Protein Domains for RAD52 Gene

InterPro:
Blocks:
  • Rad52/22 double-strand break repair protein
ProtoNet:

Suggested Antigen Peptide Sequences for RAD52 Gene

GenScript: Design optimal peptide antigens:
  • RAD52 homolog (S. cerevisiae), isoform CRA_a (D3DUP4_HUMAN)
  • RAD52 protein (Q1RMF2_HUMAN)
  • RAD52 protein (Q3MI84_HUMAN)
  • cDNA FLJ77029, highly similar to Homo sapiens RAD52 homolog (S. cerevisiae) (RAD52), transcript variant alpha, mRNA (Q5DR82_HUMAN)
  • cDNA FLJ76573, highly similar to Homo sapiens RAD52 homolog (S. cerevisiae) (RAD52), transcript variant gamma, mRNA (Q9Y5T8_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P43351

UniProtKB/Swiss-Prot:

RAD52_HUMAN :
  • Belongs to the RAD52 family.
Family:
  • Belongs to the RAD52 family.
genes like me logo Genes that share domains with RAD52: view

Function for RAD52 Gene

Molecular function for RAD52 Gene

UniProtKB/Swiss-Prot Function:
Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
GENATLAS Biochemistry:
yeast (S cerevisiae) RAD52 homolog complexing with phosphorylated BRCA1,BRCA2 and RAD51 for DNA repair of double strand breaks through homologous recombination,also involved in meiotic recombination,not involved in breast cancer

Phenotypes From GWAS Catalog for RAD52 Gene

Gene Ontology (GO) - Molecular Function for RAD52 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IDA 19506022
GO:0003697 single-stranded DNA binding IMP 12370410
GO:0005515 protein binding IPI 8702565
GO:0042802 identical protein binding IPI 10744977
genes like me logo Genes that share ontologies with RAD52: view
genes like me logo Genes that share phenotypes with RAD52: view

Animal Models for RAD52 Gene

MGI Knock Outs for RAD52:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RAD52

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RAD52 Gene

Localization for RAD52 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RAD52 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RAD52 gene
Compartment Confidence
nucleus 5
cytosol 3
cytoskeleton 2
mitochondrion 2
plasma membrane 1
extracellular 1
peroxisome 1
endoplasmic reticulum 1
endosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nuclear speckles (2)
  • Plasma membrane (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for RAD52 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 19506022
GO:0005654 nucleoplasm TAS --
GO:0032991 protein-containing complex IDA 19506022
GO:0032993 protein-DNA complex IMP 12370410
genes like me logo Genes that share ontologies with RAD52: view

Pathways & Interactions for RAD52 Gene

genes like me logo Genes that share pathways with RAD52: view

SIGNOR curated interactions for RAD52 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for RAD52 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000724 double-strand break repair via homologous recombination IBA 21873635
GO:0000730 DNA recombinase assembly IEA --
GO:0006281 DNA repair IEA --
GO:0006302 double-strand break repair TAS 7774919
GO:0006310 DNA recombination IMP 8702565
genes like me logo Genes that share ontologies with RAD52: view

Drugs & Compounds for RAD52 Gene

(3) Drugs for RAD52 Gene - From: PharmGKB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Cisplatin Approved Pharma Inhibits DNA synthesis,chemotherapy drug, Potent pro-apoptotic anticancer agent; activates caspase-3, Platinum 3412
Cyclophosphamide Approved, Investigational Pharma Nitrogen mustard alkylating agent and prodrug. 3670

(1) Additional Compounds for RAD52 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with RAD52: view

Transcripts for RAD52 Gene

mRNA/cDNA for RAD52 Gene

7 REFSEQ mRNAs :
23 NCBI additional mRNA sequence :
19 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for RAD52

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for RAD52 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b · 4c · 4d · 4e ^ 5 ^ 6a · 6b · 6c · 6d ^ 7 ^ 8 ^ 9 ^ 10a · 10b · 10c · 10d ^ 11a · 11b · 11c ^
SP1: - - - - - - - - - - - - - - - - -
SP2: - - - -
SP3: - - - - - -
SP4: - - - - -
SP5:
SP6:
SP7: - -
SP8: -
SP9: -
SP10:
SP11: - - -
SP12: - - - -
SP13: - - - - - - - -
SP14:
SP15: -
SP16:

ExUns: 12a · 12b ^ 13 ^ 14 ^ 15a · 15b · 15c · 15d ^ 16a · 16b ^ 17a · 17b ^ 18a · 18b
SP1: - - - - -
SP2: - - -
SP3: - - - - -
SP4: - -
SP5: - - - -
SP6:
SP7:
SP8:
SP9:
SP10:
SP11:
SP12:
SP13:
SP14: -
SP15:
SP16: - -

Relevant External Links for RAD52 Gene

GeneLoc Exon Structure for
RAD52

Expression for RAD52 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for RAD52 Gene

Protein differential expression in normal tissues from HIPED for RAD52 Gene

This gene is overexpressed in Lung (44.8) and Esophagus (24.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for RAD52 Gene



Protein tissue co-expression partners for RAD52 Gene

- Elite partner

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for RAD52

SOURCE GeneReport for Unigene cluster for RAD52 Gene:

Hs.410355

Evidence on tissue expression from TISSUES for RAD52 Gene

  • Nervous system(4.4)
  • Spleen(4.3)
  • Skin(2.7)
  • Intestine(2.5)
  • Blood(2.4)
  • Kidney(2.1)
genes like me logo Genes that share expression patterns with RAD52: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for RAD52 Gene

Orthologs for RAD52 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RAD52 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia RAD52 30 31
  • 98.16 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia RAD52 30 31
  • 82.03 (n)
OneToOne
Cow
(Bos Taurus)
Mammalia RAD52 30 31
  • 79.55 (n)
OneToOne
Mouse
(Mus musculus)
Mammalia Rad52 30 17 31
  • 77.5 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Rad52 30
  • 74.17 (n)
Platypus
(Ornithorhynchus anatinus)
Mammalia RAD52 31
  • 73 (a)
OneToOne
Oppossum
(Monodelphis domestica)
Mammalia RAD52 31
  • 57 (a)
OneToOne
Chicken
(Gallus gallus)
Aves RAD52 30 31
  • 64.4 (n)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia RAD52 31
  • 81 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia rad52 30
  • 60.51 (n)
Str.20423 30
Zebrafish
(Danio rerio)
Actinopterygii rad52 30 31
  • 56.32 (n)
OneToOne
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RAD52 31 33
  • 24 (a)
OneToOne
Species where no ortholog for RAD52 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Fruit Fly (Drosophila melanogaster)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Squirt (Ciona savignyi)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for RAD52 Gene

ENSEMBL:
Gene Tree for RAD52 (if available)
TreeFam:
Gene Tree for RAD52 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RAD52: view image

Paralogs for RAD52 Gene

Pseudogenes.org Pseudogenes for RAD52 Gene

genes like me logo Genes that share paralogs with RAD52: view

No data available for Paralogs for RAD52 Gene

Variants for RAD52 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for RAD52 Gene

SNP ID Clinical significance and condition Chr 12 pos Variation AA Info Type
781058 Benign: not provided 916,703(-) G/C MISSENSE_VARIANT,NON_CODING_TRANSCRIPT_VARIANT
884159 Benign: Pseudohypoaldosteronism type 2C 911,028(-) T/C THREE_PRIME_UTR_VARIANT
rs104895054 Not Provided: not provided 912,197(-) G/A NON_CODING_TRANSCRIPT_VARIANT,THREE_PRIME_UTR_VARIANT
rs104895055 Not Provided: not provided 912,399(-) A/C NON_CODING_TRANSCRIPT_VARIANT,THREE_PRIME_UTR_VARIANT
rs104895056 Not Provided: not provided 912,923(-) G/A NON_CODING_TRANSCRIPT_VARIANT,THREE_PRIME_UTR_VARIANT

Additional dbSNP identifiers (rs#s) for RAD52 Gene

Structural Variations from Database of Genomic Variants (DGV) for RAD52 Gene

Variant ID Type Subtype PubMed ID
dgv2251n54 CNV gain 21841781
dgv255e199 CNV deletion 23128226
dgv747n106 CNV deletion 24896259
esv2422010 CNV deletion 20811451
esv2639640 CNV deletion 19546169
esv2673796 CNV deletion 23128226
esv2745370 CNV deletion 23290073
esv2745371 CNV deletion 23290073
esv2745372 CNV deletion 23290073
esv3254496 CNV deletion 24192839
esv3548605 CNV deletion 23714750
esv3628261 CNV gain 21293372
esv3628262 CNV loss 21293372
esv3628264 CNV loss 21293372
esv3628266 CNV loss 21293372
nsv1042333 CNV gain 25217958
nsv1119926 CNV deletion 24896259
nsv1153006 CNV deletion 26484159
nsv468962 CNV gain 19166990
nsv516003 CNV loss 19592680
nsv521937 CNV loss 19592680
nsv556942 CNV gain 21841781
nsv818878 CNV loss 17921354
nsv957404 CNV deletion 24416366

Variation tolerance for RAD52 Gene

Residual Variation Intolerance Score: 63.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.97; 49.51% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RAD52 Gene

Human Gene Mutation Database (HGMD)
RAD52
SNPedia medical, phenotypic, and genealogical associations of SNPs for
RAD52

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RAD52 Gene

Disorders for RAD52 Gene

MalaCards: The human disease database

(7) MalaCards diseases for RAD52 Gene - From: DISEASES, Novoseek, and GeneCards

- elite association - COSMIC cancer census association via MalaCards
Search RAD52 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for RAD52

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with RAD52: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RAD52 Gene

Publications for RAD52 Gene

  1. The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues. (PMID: 7774919) Shen Z … Chen DJ (Genomics 1995) 2 3 4 23
  2. The recombination protein RAD52 cooperates with the excision repair protein OGG1 for the repair of oxidative lesions in mammalian cells. (PMID: 19506022) de Souza-Pinto NC … Bohr VA (Molecular and cellular biology 2009) 2 3 23
  3. DNA repair synthesis facilitates RAD52-mediated second-end capture during DSB repair. (PMID: 18313388) McIlwraith MJ … West SC (Molecular cell 2008) 2 3 23
  4. Variants in DNA double-strand break repair and DNA damage-response genes and susceptibility to lung and head and neck cancers. (PMID: 18449888) Danoy P … Benhamou S (International journal of cancer 2008) 3 23 41
  5. Genotype-phenotype relationship between DNA repair gene genetic polymorphisms and DNA repair capacity. (PMID: 18990028) Shin A … Kang D (Asian Pacific journal of cancer prevention : APJCP 2008) 3 23 41

Products for RAD52 Gene

Sources for RAD52 Gene