RAB35 Gene (Protein Coding)

RAB35, Member RAS Oncogene Family

GC12M120096
GIFtS:
42

Aliases for RAB35 Gene

Aliases for RAB35 Gene

  • RAB35, Member RAS Oncogene Family 2 3 5
  • Ras-Related Protein Rab-35 3 4
  • GTP-Binding Protein RAY 3 4
  • RAB1C 3 4
  • RAY 3 4
  • Ras-Related Protein Rab-1c (GTP-Binding Protein Ray) 3
  • Ras-Related Protein Rab-1C 4
  • H-Ray 3

External Ids for RAB35 Gene

Previous GeneCards Identifiers for RAB35 Gene

  • GC12M119405
  • GC12M120060
  • GC12M120315
  • GC12M118944
  • GC12M118995
  • GC12M120532
  • GC12M117542

Summaries for RAB35 Gene

GeneCards Summary for RAB35 Gene

RAB35 (RAB35, Member RAS Oncogene Family) is a Protein Coding gene. Diseases associated with RAB35 include Hypotrichosis 3 and Charcot-Marie-Tooth Disease, Axonal, Type 2L. Among its related pathways are Vesicle-mediated transport and Cytoskeletal Signaling. Gene Ontology (GO) annotations related to this gene include GTP binding and GDP binding. An important paralog of this gene is RAB1A.

UniProtKB/Swiss-Prot Summary for RAB35 Gene

  • The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.

Gene Wiki entry for RAB35 Gene

Additional gene information for RAB35 Gene

No data available for Entrez Gene Summary , CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for RAB35 Gene

Genomics for RAB35 Gene

GeneHancer (GH) Regulatory Elements for RAB35 Gene

Promoters and enhancers for RAB35 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around RAB35 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the RAB35 gene promoter:
  • c-Ets-1
  • CREB
  • deltaCREB
  • GATA-2
  • NF-1
  • NF-1/L
  • p53
  • POU2F1
  • POU2F1a
  • STAT3

Genomic Locations for RAB35 Gene

Genomic Locations for RAB35 Gene
chr12:120,095,094-120,117,502
(GRCh38/hg38)
Size:
22,409 bases
Orientation:
Minus strand
chr12:120,532,899-120,555,306
(GRCh37/hg19)
Size:
22,408 bases
Orientation:
Minus strand

Genomic View for RAB35 Gene

Genes around RAB35 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
RAB35 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for RAB35 Gene
GeneLoc Logo Genomic Neighborhood • Exon Structure • Gene Density

RefSeq DNA sequence for RAB35 Gene

Proteins for RAB35 Gene

Products:

  1. Antibody
  2. Protein
  3. Assay

  • Protein details for RAB35 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q15286-RAB35_HUMAN
    Recommended name:
    Ras-related protein Rab-35
    Protein Accession:
    Q15286
    Secondary Accessions:
    • B2R6E0
    • B4E390

    Protein attributes for RAB35 Gene

    Size:
    201 amino acids
    Molecular mass:
    23025 Da
    Quaternary structure:
    • Interacts with DENND1A and DENND1B; in a nucleotide-dependent manner (PubMed:20154091, PubMed:22065758). Interacts with DENND1C; weak interaction which is nucleotide-independent (PubMed:20154091). Interacts (GTP-bound form) with ACAP2 and MICALL1; the interaction is direct and probably recruits ACAP2 and MICALL1 to membranes (PubMed:21951725). Interacts with EHD1; the interaction is indirect through MICALL1 and probably recruits EHD1 to membranes (By similarity). Interacts with GDI1, GDI2, CHM and CHML; phosphorylation at Thr-72 disrupts these interactions (PubMed:29125462).

    Three dimensional structures from OCA and Proteopedia for RAB35 Gene

    Alternative splice isoforms for RAB35 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for RAB35 Gene

Protein Expression for RAB35 Gene

See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB

Post-translational modifications for RAB35 Gene

  • AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB35 constitutively active.
  • Phosphocholinated by L.pneumophila AnkX. Both GDP-bound and GTP-bound forms can be phosphocholinated. Phosphocholination inhibits the GEF activity of DENND1A.
  • Ubiquitination at Lys178
  • Modification sites at PhosphoSitePlus

Other Protein References for RAB35 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for RAB35 Gene

Domains & Families for RAB35 Gene

Gene Families for RAB35 Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for RAB35 Gene

Blocks:
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for RAB35 Gene

GenScript: Design optimal peptide antigens:
  • cDNA FLJ57794, moderately similar to Ras-related protein Rab-35 (B4DTA6_HUMAN)
  • cDNA FLJ57783, highly similar to Ras-related protein Rab-35 (B4E390_HUMAN)
  • Ras-related protein Rab-1C (RAB35_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q15286

UniProtKB/Swiss-Prot:

RAB35_HUMAN :
  • Belongs to the small GTPase superfamily. Rab family.
Family:
  • Belongs to the small GTPase superfamily. Rab family.
genes like me logo Genes that share domains with RAB35: view

Function for RAB35 Gene

Products:

  1. Animal Model
  2. CRISPR
  3. miRNA
  4. Inhibitory RNA
  5. Clone
  6. Cell Line

Molecular function for RAB35 Gene

UniProtKB/Swiss-Prot Function:
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.
UniProtKB/Swiss-Prot EnzymeRegulation:
Rab activation is generally mediated by a guanine exchange factor (GEF), while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP) (PubMed:20154091). That Rab is activated by the guanine exchange factors DENND1A, DENND1B and DENND1C (PubMed:20154091).
GENATLAS Biochemistry:
RAB35,RAS oncogene superfamily member 35,ubiquitously expressed

Phenotypes From GWAS Catalog for RAB35 Gene

Gene Ontology (GO) - Molecular Function for RAB35 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0003924 GTPase activity IDA,IEA 17562788
GO:0005515 protein binding IPI 17562788
GO:0005525 GTP binding IEA,IDA 20937701
GO:0005546 phosphatidylinositol-4,5-bisphosphate binding IDA 16950109
genes like me logo Genes that share ontologies with RAB35: view
genes like me logo Genes that share phenotypes with RAB35: view

Animal Models for RAB35 Gene

MGI Knock Outs for RAB35:
  • Rab35 Rab35<tm1b(EUCOMM)Hmgu>

Animal Model Products

CRISPR Products

miRNA for RAB35 Gene

miRTarBase miRNAs that target RAB35

Clone Products

  • Addgene plasmids for RAB35

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for RAB35 Gene

Localization for RAB35 Gene

Subcellular locations from UniProtKB/Swiss-Prot for RAB35 Gene

Cell membrane; Lipid-anchor; Cytoplasmic side. Membrane, clathrin-coated pit. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome. Melanosome. Note=Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065). {ECO:0000269 PubMed:16950109, ECO:0000269 PubMed:17081065}.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for RAB35 gene
Compartment Confidence
plasma membrane 5
extracellular 5
cytosol 5
endosome 4
golgi apparatus 3
cytoskeleton 1
mitochondrion 1
nucleus 1
lysosome 1

Gene Ontology (GO) - Cellular Components for RAB35 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane IBA 21873635
GO:0005768 endosome IEA --
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane IBA,IDA 16950109
GO:0005905 clathrin-coated pit IDA,IEA 16950109
genes like me logo Genes that share ontologies with RAB35: view

No data available for Subcellular locations from the Human Protein Atlas (HPA) for RAB35 Gene

Pathways & Interactions for RAB35 Gene

genes like me logo Genes that share pathways with RAB35: view

Pathways by source for RAB35 Gene

1 KEGG pathway for RAB35 Gene
1 Cell Signaling Technology pathway for RAB35 Gene

Gene Ontology (GO) - Biological Process for RAB35 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000281 mitotic cytokinesis IMP 16950109
GO:0006886 intracellular protein transport IBA 21873635
GO:0008104 protein localization IMP 16950109
GO:0015031 protein transport IEA --
GO:0016197 endosomal transport IMP 16950109
genes like me logo Genes that share ontologies with RAB35: view

No data available for SIGNOR curated interactions for RAB35 Gene

Drugs & Compounds for RAB35 Gene

Products:

  1. Drug
No Compound Related Data Available

Transcripts for RAB35 Gene

Products:

  1. CRISPR
  2. miRNA
  3. Inhibitory RNA
  4. Clone

mRNA/cDNA for RAB35 Gene

(2) REFSEQ mRNAs :
(20) Additional mRNA sequences :
(11) Selected AceView cDNA sequences:
(6) Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Clone Products

  • Addgene plasmids for RAB35

Alternative Splicing Database (ASD) splice patterns (SP) for RAB35 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b · 8c
SP1: - -
SP2: - - -
SP3: - - - -
SP4: -
SP5: - -

Relevant External Links for RAB35 Gene

GeneLoc Exon Structure for
RAB35
ECgene alternative splicing isoforms for
RAB35

Expression for RAB35 Gene

Products:

  1. Primer
  2. Gene Expression Assay

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for RAB35 Gene

mRNA expression in normal human tissues for RAB35 Gene
mRNA expression by GTEx for RAB35 GenemRNA expression by BioGPS for RAB35 Gene

Protein differential expression in normal tissues from HIPED for RAB35 Gene

This gene is overexpressed in Brain (6.3) and Uterus (6.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for RAB35 Gene



Protein tissue co-expression partners for RAB35 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of RAB35 Gene:

RAB35

SOURCE GeneReport for Unigene cluster for RAB35 Gene:

Hs.524788

Evidence on tissue expression from TISSUES for RAB35 Gene

  • Nervous system(4.8)
  • Muscle(4.2)
genes like me logo Genes that share expression patterns with RAB35: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for RAB35 Gene

Orthologs for RAB35 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for RAB35 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
 Mammalia RAB35 33 32
  • 99.83 (n)
 OneToOne
dog
(Canis familiaris)
 Mammalia RAB35 33 32
  • 94.03 (n)
 OneToOne
mouse
(Mus musculus)
 Mammalia Rab35 17 33 32
  • 93.86 (n)
rat
(Rattus norvegicus)
 Mammalia Rab35 32
  • 93.86 (n)
cow
(Bos Taurus)
 Mammalia RAB35 33 32
  • 93.2 (n)
 OneToOne
oppossum
(Monodelphis domestica)
 Mammalia RAB35 33
  • 93 (a)
 OneToOne
chicken
(Gallus gallus)
 Aves RAB35 33 32
  • 84.74 (n)
 OneToOne
lizard
(Anolis carolinensis)
 Reptilia RAB35 33
  • 100 (a)
 OneToOne
tropical clawed frog
(Silurana tropicalis)
 Amphibia rab35 32
  • 83.25 (n)
African clawed frog
(Xenopus laevis)
 Amphibia Xl.16236 32
zebrafish
(Danio rerio)
 Actinopterygii RAB35 (3 of 3) 33
  • 89 (a)
 OneToMany
rab35b 33 32
  • 80.93 (n)
 OneToMany
BX571714.2 33
  • 69 (a)
 OneToMany
African malaria mosquito
(Anopheles gambiae)
 Insecta AgaP_AGAP003028 32
  • 69.98 (n)
fruit fly
(Drosophila melanogaster)
 Insecta Rab35 33 32
  • 68.52 (n)
 OneToOne
CG9575 34
  • 66 (a)
CG2885 34
  • 44 (a)
worm
(Caenorhabditis elegans)
 Secernentea rab-35 33 32
  • 64.16 (n)
 OneToOne
4R79.2 34
  • 39 (a)
baker's yeast
(Saccharomyces cerevisiae)
 Saccharomycetes SEC4 33
  • 43 (a)
 OneToMany
sea squirt
(Ciona savignyi)
 Ascidiacea CSA.7112 33
  • 88 (a)
 OneToOne
Species where no ortholog for RAB35 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for RAB35 Gene

ENSEMBL:
Gene Tree for RAB35 (if available)
TreeFam:
Gene Tree for RAB35 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for RAB35: view image

Paralogs for RAB35 Gene

Paralogs for RAB35 Gene

(105) SIMAP similar genes for RAB35 Gene using alignment to 4 proteins:

  • RAB35_HUMAN
  • F5H157_HUMAN
  • F5H7F8_HUMAN
  • G5E9K9_HUMAN
genes like me logo Genes that share paralogs with RAB35: view

Variants for RAB35 Gene

Products:

  1. SNP Genotyping and Copy Number Assay

Sequence variations from dbSNP and Humsavar for RAB35 Gene

SNP ID Clin Chr 12 pos Variation AA Info Type
rs1000121048 -- 120,094,819(-) G/A downstream_transcript_variant
rs1000329592 -- 120,100,004(-) G/A intron_variant
rs1000473461 -- 120,105,259(-) G/A/C intron_variant
rs1000527192 -- 120,104,923(-) C/A intron_variant
rs1000692820 -- 120,116,818(-) C/G 5_prime_UTR_variant, genic_upstream_transcript_variant, upstream_transcript_variant

Structural Variations from Database of Genomic Variants (DGV) for RAB35 Gene

Variant ID Type Subtype PubMed ID
nsv519657 CNV loss 19592680
nsv560433 CNV loss 21841781
nsv560434 CNV loss 21841781
nsv952492 CNV deletion 24416366
nsv952493 CNV deletion 24416366

Variation tolerance for RAB35 Gene

Residual Variation Intolerance Score: 49.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.93; 59.52% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for RAB35 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
RAB35

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for RAB35 Gene

Disorders for RAB35 Gene

MalaCards: The human disease database

(2) MalaCards diseases for RAB35 Gene - From: DISEASES

Disorder Aliases PubMed IDs
hypotrichosis 3
  • hypt3
charcot-marie-tooth disease, axonal, type 2l
  • cmt2l
- elite association - COSMIC cancer census association via MalaCards
Search RAB35 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for RAB35

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
genes like me logo Genes that share disorders with RAB35: view

No data available for UniProtKB/Swiss-Prot and Genatlas for RAB35 Gene

Publications for RAB35 Gene

  1. MICAL-L1 is a tubular endosomal membrane hub that connects Rab35 and Arf6 with Rab8a. (PMID: 21951725) Rahajeng J … Caplan S (Traffic (Copenhagen, Denmark) 2012) 3 4 56
  2. Insights regarding guanine nucleotide exchange from the structure of a DENN-domain protein complexed with its Rab GTPase substrate. (PMID: 22065758) Wu X … Reinisch KM (Proceedings of the National Academy of Sciences of the United States of America 2011) 3 4 56
  3. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PMID: 20379614) Rose JE … Uhl GR (Molecular medicine (Cambridge, Mass.) 2010) 3 43 56
  4. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PMID: 20877624) Hendrickson SL … O'Brien SJ (PloS one 2010) 3 43 56
  5. The finished DNA sequence of human chromosome 12. (PMID: 16541075) Scherer SE … Baylor College of Medicine Human Genome Sequencing Center Sequence Production Team (Nature 2006) 3 4 56

ExternalLinks

View latest publications for RAB35 gene in Mastermind

Products for RAB35 Gene

  • Addgene plasmids for RAB35

Sources for RAB35 Gene