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The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 n... See more...
Aliases for PSME3 Gene
Aliases for PSME3 Gene
- Proteasome Activator Subunit 3 2 3 5
- Proteasome (Prosome, Macropain) Activator Subunit 3 (PA28 Gamma; Ki) 2 3
- Activator Of Multicatalytic Protease Subunit 3 3 4
- Proteasome Activator Complex Subunit 3 3 4
- Proteasome Activator 28 Subunit Gamma 3 4
- 11S Regulator Complex Subunit Gamma 3 4
- Ki Nuclear Autoantigen 3 4
- PA28-Gamma 2 3
- PA28gamma 3 4
- REG-GAMMA 2 3
- PA28G 2 3
External Ids for PSME3 Gene
- HGNC: 9570
- Entrez Gene: 10197
- Ensembl: ENSG00000131467
- OMIM: 605129
- UniProtKB: P61289
Previous GeneCards Identifiers for PSME3 Gene
- GC17M040635
- GC17P043138
- GC17P040885
- GC17P041358
- GC17P038238
- GC17P040985
- GC17P036749
Summaries for PSME3 Gene
-
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]
GeneCards Summary for PSME3 Gene
PSME3 (Proteasome Activator Subunit 3) is a Protein Coding gene. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Antigen processing-Cross presentation. Gene Ontology (GO) annotations related to this gene include identical protein binding and MDM2/MDM4 family protein binding. An important paralog of this gene is PSME1.
UniProtKB/Swiss-Prot Summary for PSME3 Gene
-
Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978).
Additional gene information for PSME3 Gene
- HGNC(9570)
- Entrez Gene(10197)
- Ensembl(ENSG00000131467)
- OMIM(605129)
- UniProtKB(P61289)
- Open Targets Platform(ENSG00000131467)
- Monarch Initiative
- Search for PSME3 at DataMed
- Search for PSME3 at HumanCyc
No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for PSME3 Gene
Genomics for PSME3 Gene
GeneHancer (GH) Regulatory Elements Pubs
Promoters and enhancers for PSME3 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication |
Request up-to-date GeneHancer data (full dataset)
GeneHancers around PSME3 on the GeneHancer Hub at the UCSC Golden Path
Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for PSME3
- Top Transcription factor binding sites by QIAGEN in the PSME3 gene promoter:
-
- AP-1
- GR
- GR-alpha
- GR-beta
- p53
Genomic Locations for PSME3 Gene
Genomic Locations for PSME3 Gene
- chr17:42,824,385-42,843,760
- (GRCh38/hg38)
- Size:
- 19,376 bases
- Orientation:
- Plus strand
- chr17:40,976,402-40,995,777
- (GRCh37/hg19)
- Size:
- 19,376 bases
- Orientation:
- Plus strand
Genomic View for PSME3 Gene
Genes around PSME3 on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 17q21.31 by HGNC
- 17q21.31 by Entrez Gene
- 17q21.31 by Ensembl
PSME3 Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for PSME3 Gene
Proteins for PSME3 Gene
-
Protein details for PSME3 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- P61289-PSME3_HUMAN
- Recommended name:
- Proteasome activator complex subunit 3
- Protein Accession:
- P61289
- A8K9A3
- O35563
- P97373
- Q12920
- Q13172
- Q9BQD9
Protein attributes for PSME3 Gene
- Size:
- 254 amino acids
- Molecular mass:
- 29506 Da
- Quaternary structure:
-
- Homoheptamer; the stability of the heptamer is essential for the specific activation of the trypsine-like subunit and inhibition of the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits of the proteasome. Interacts with p53/TP53 and MDM2. Interacts with MAP3K3 (By similarity). Associates with the proteasome. Interacts with CCAR2. Interacts with PSME3IP1 (via C-terminus); the interaction is direct and promotes the association of PSME3 with the 20S proteasome (PubMed:29934401). Interacts with COIL; the interaction is inhibited by PSME3IP1 (PubMed:29934401).
- (Microbial infection) Interacts with human cytomegalovirus UL27.
Alternative splice isoforms for PSME3 Gene
- UniProtKB/Swiss-Prot:
Protein Expression for PSME3 Gene
Post-translational modifications for PSME3 Gene
- Phosphorylated by MAP3K3 (By similarity). Phosphorylation at Ser-247 promotes its association with CCAR2.
- Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1.
-
Ubiquitination at Lys6, Lys14, Lys37, Lys100, Lys110, Lys121, and Lys195
- NX_P61289 (NX_P61289-1)
- Modification sites at PhosphoSitePlus
Other Protein References for PSME3 Gene
Antibody Products
- G Biosciences Immunotag™ PSME3 Antibody
- Creative Diagnostics Anti-PSME3 (aa 204-254) polyclonal antibody
- Novus Biologicals Antibodies for PSME3
-
Abcam antibodies for PSME3
- Invitrogen Antibodies for PSME3 (AFLGC-PSME3)
-
Boster Bio Antibodies for PSME3
-
Santa Cruz Biotechnology (SCBT) Antibodies for PSME3
- Sino Biological Antibodies for PSME3
Protein Products
-
OriGene Purified Proteins for PSME3
- Search Origene for MassSpec and Protein Over-expression Lysates for PSME3
- Origene Custom Protein Services for PSME3
- Sino Biological Recombinant Proteins for PSME3
- Browse Sino Biological Cell Lysates
- ProSpec Recombinant Proteins for PSME3
-
Signalway Proteins for PSME3
-
ProSci Proteins for PSME3
-
Abcam proteins for PSME3
Assay Products
No data available for DME Specific Peptides for PSME3 Gene
Domains & Families for PSME3 Gene
Gene Families for PSME3 Gene
- HGNC:
- Human Protein Atlas (HPA):
-
- Predicted intracellular proteins
Protein Domains for PSME3 Gene
- InterPro:
- Blocks:
-
- Proteasome activator pa28, alpha subunit
- ProtoNet:
Suggested Antigen Peptide Sequences for PSME3 Gene
- GenScript: Design optimal peptide antigens:
-
- cDNA FLJ32659 fis, clone TESTI1000045, highly similar to Proteasome activator complex subunit 3 (B3KQ25_HUMAN)
- cDNA FLJ57249, highly similar to Proteasome activator complex subunit 3 (B7Z8D3_HUMAN)
- Proteasome activator 28 subunit gamma (PSME3_HUMAN)
- PSME3 protein (Q6FHK7_HUMAN)
Graphical View of Domain Structure for InterPro Entry
P61289UniProtKB/Swiss-Prot:
PSME3_HUMAN :- The C-terminal sequences affect heptamer stability and proteasome affinity.
- Belongs to the PA28 family.
- Domain:
-
- The C-terminal sequences affect heptamer stability and proteasome affinity.
- Family:
-
- Belongs to the PA28 family.
Function for PSME3 Gene
Molecular function for PSME3 Gene
- UniProtKB/Swiss-Prot Function:
- Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978).
- UniProtKB/Swiss-Prot Induction:
- Up-regulated in thyroid carcinoma cells.
- GENATLAS Biochemistry:
- proteasome (prosome,macropain) activator subunit 3 (PA28-gamma)
Phenotypes From GWAS Catalog for PSME3 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0002039 | p53 binding | IDA | 18309296 |
| GO:0005515 | protein binding | IPI | 12970408 |
| GO:0042802 | identical protein binding | IPI | 16169070 |
| GO:0061133 | endopeptidase activator activity | IBA,IDA | 18309296 |
| GO:0097371 | MDM2/MDM4 family protein binding | IDA | 18309296 |
Phenotypes for PSME3 Gene
- MGI mutant phenotypes for PSME3:
- inferred from 2 alleles
- GenomeRNAi human phenotypes for PSME3:
Animal Model Products
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
CRISPR Products
- Applied Biological Materials (abm): CRISPR Clones for PSME3 - Select products 50% OFF >
- * PSME3 CRISPR as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
-
OriGene CRISPR knockouts for PSME3
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for PSME3
-
Santa Cruz Biotechnology (SCBT) CRISPR for PSME3
miRNA for PSME3 Gene
- miRTarBase miRNAs that target PSME3
-
- hsa-miR-7-5p (MIRT004063)
- hsa-miR-155-5p (MIRT020956)
- hsa-miR-124-3p (MIRT022991)
- hsa-miR-24-3p (MIRT030577)
- hsa-miR-16-5p (MIRT031898)
- hsa-let-7b-5p (MIRT032477)
- hsa-miR-484 (MIRT042151)
- hsa-miR-324-5p (MIRT043211)
- hsa-miR-331-3p (MIRT043475)
- hsa-miR-583 (MIRT077242)
- hsa-miR-548au-3p (MIRT251100)
- hsa-miR-539-5p (MIRT287286)
- hsa-miR-3117-5p (MIRT287289)
- hsa-miR-4311 (MIRT287291)
- hsa-miR-6758-5p (MIRT375623)
- hsa-miR-6856-5p (MIRT375624)
- hsa-miR-6734-5p (MIRT470135)
- hsa-miR-7111-5p (MIRT470136)
- hsa-miR-6870-5p (MIRT470137)
- hsa-miR-4723-5p (MIRT470138)
- hsa-miR-5698 (MIRT470139)
- hsa-miR-3175 (MIRT470140)
- hsa-miR-8085 (MIRT470141)
- hsa-miR-6731-5p (MIRT470142)
- hsa-miR-6878-5p (MIRT470143)
- hsa-miR-6797-5p (MIRT470144)
- hsa-miR-1249-5p (MIRT470145)
- hsa-miR-4644 (MIRT470146)
- hsa-miR-4306 (MIRT470147)
- hsa-miR-185-5p (MIRT470148)
- hsa-miR-6133 (MIRT470149)
- hsa-miR-6130 (MIRT470150)
- hsa-miR-6129 (MIRT470151)
- hsa-miR-6127 (MIRT470152)
- hsa-miR-4510 (MIRT470153)
- hsa-miR-4419a (MIRT470154)
- hsa-miR-570-3p (MIRT470155)
- hsa-miR-4459 (MIRT470156)
- hsa-miR-6794-5p (MIRT470157)
- hsa-miR-4716-3p (MIRT470158)
- hsa-miR-6760-5p (MIRT470159)
- hsa-miR-4779 (MIRT470160)
- hsa-miR-6768-5p (MIRT470161)
- hsa-miR-186-3p (MIRT564118)
- hsa-miR-5692a (MIRT564119)
- hsa-miR-1276 (MIRT564120)
- mmu-miR-3473a (MIRT581557)
- mmu-miR-882 (MIRT581558)
- mmu-miR-185-5p (MIRT581559)
- hsa-miR-7156-5p (MIRT711281)
- hsa-miR-4273 (MIRT711282)
- hsa-miR-519e-5p (MIRT711283)
- hsa-miR-515-5p (MIRT711284)
- hsa-miR-2117 (MIRT711285)
- hsa-miR-26b-3p (MIRT711286)
- hsa-miR-6861-5p (MIRT711287)
- hsa-miR-6862-3p (MIRT711288)
- hsa-miR-6784-3p (MIRT711289)
- hsa-miR-6742-3p (MIRT711290)
- hsa-miR-6791-5p (MIRT711291)
- hsa-miR-4292 (MIRT711292)
- hsa-miR-3620-3p (MIRT711293)
- hsa-miR-6865-3p (MIRT711294)
- hsa-miR-504-5p (MIRT711295)
- hsa-miR-6750-3p (MIRT711296)
- hsa-miR-425-3p (MIRT724085)
- hsa-miR-1206 (MIRT724086)
- hsa-miR-503-3p (MIRT724087)
- hsa-miR-34c-3p (MIRT724088)
- hsa-miR-32-5p (MIRT726845)
- hsa-miR-92b-3p (MIRT726846)
- hsa-miR-92a-3p (MIRT726847)
- hsa-miR-1273h-5p (MIRT736891)
- hsa-miR-1322 (MIRT737383)
- hsa-miR-139-5p (MIRT737560)
- hsa-miR-30b-3p (MIRT739781)
- hsa-miR-30c-1-3p (MIRT739834)
- hsa-miR-30c-2-3p (MIRT739877)
- hsa-miR-3662 (MIRT741997)
- hsa-miR-3689a-3p (MIRT742283)
- hsa-miR-3689b-3p (MIRT742353)
- hsa-miR-3689c (MIRT742423)
- hsa-miR-4684-5p (MIRT746949)
- hsa-miR-548c-3p (MIRT750678)
- hsa-miR-6779-5p (MIRT755525)
- hsa-miR-6780a-5p (MIRT755598)
- hsa-miR-6788-5p (MIRT755986)
- hsa-miR-887-5p (MIRT760413)
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for PSME3
- Browse OriGene Inhibitory RNA Products For PSME3
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PSME3
Clone Products
- Applied Biological Materials (abm): Clones for PSME3 - Select products 50% OFF >
- * PSME3 as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
-
- MG203255
- MR203255
- MR203255L3
- MR203255L4
- RC200308
- RC200308L1
- RC200308L2
- RC200308L3
- RC200308L4
- RC222312
- RC222312L1
- RC222312L2
- RC222312L3
- RC222312L4
- RC232329
- RG200308
- RG222312
- RG232329
- RR215566
- RR215566L3
- RR215566L4
- MR203255L3V
- MR203255L4V
- RC200308L1V
- RC200308L2V
- RC200308L3V
- RC200308L4V
- RC222312L1V
- RC222312L2V
- RC222312L3V
- RC222312L4V
- RR215566L3V
- RR215566L4V
- Custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
- Sino Biological Human cDNA Clone for PSME3
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for PSME3
Cell Line Products
-
Horizon Cell Lines for PSME3
No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PSME3 Gene
Localization for PSME3 Gene
Subcellular locations from UniProtKB/Swiss-Prot for PSME3 Gene
- Nucleus. Cytoplasm. Note=Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase. {ECO:0000250}.
| Compartment | Confidence |
|---|---|
| nucleus | 5 |
| cytosol | 5 |
| extracellular | 2 |
| mitochondrion | 2 |
| plasma membrane | 1 |
| cytoskeleton | 1 |
| peroxisome | 1 |
| endoplasmic reticulum | 1 |
| endosome | 1 |
| lysosome | 1 |
| golgi apparatus | 1 |
- Nucleoplasm (4)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000502 | proteasome complex | TAS | 8811196 |
| GO:0005634 | nucleus | IEA,HDA | 21630459 |
| GO:0005654 | nucleoplasm | IDA,TAS | -- |
| GO:0005737 | cytoplasm | IBA | 21873635 |
| GO:0005829 | cytosol | IDA,TAS | -- |
Pathways & Interactions for PSME3 Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Regulation of activated PAK-2p34 by proteasome mediated degradation |
.93
.93
|
.80
.79
.78
.69
.47
|
| 2 | RET signaling |
.92
|
.92
|
| 3 | HIV Life Cycle |
.65
|
.45
|
| 4 | Class I MHC mediated antigen processing and presentation | ||
| 5 | Antigen processing-Cross presentation |
Immune response Antigen presentation by MHC class I
.51
|
|
Pathways by source for PSME3 Gene
1 BioSystems pathway for PSME3 Gene
3 KEGG pathways for PSME3 Gene
1 GeneGo (Thomson Reuters) pathway for PSME3 Gene
- Immune response Antigen presentation by MHC class I
Interacting Proteins for PSME3 Gene
Selected Interacting proteins:
P61289-PSME3_HUMAN
ENSP00000293362
for PSME3 Gene via
UniProtKB
MINT
STRING
IID
GPS-Prot Interaction Network for PSME3
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000165 | MAPK cascade | TAS | -- |
| GO:0000209 | protein polyubiquitination | TAS | -- |
| GO:0002223 | stimulatory C-type lectin receptor signaling pathway | TAS | -- |
| GO:0002479 | antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent | TAS | -- |
| GO:0006521 | regulation of cellular amino acid metabolic process | TAS | -- |
No data available for SIGNOR curated interactions for PSME3 Gene
Drugs & Compounds for PSME3 Gene
| Name | Status | Disease Links | Group | Role | Mechanism of Action | Clinical Trials |
|---|---|---|---|---|---|---|
| Bortezomib | Approved, Investigational | Pharma | inhibitor, other | Proteosome and NF-kappaB inhibitor, Other, Proteasome inhibitors | 910 | |
| carfilzomib | Approved, Investigational | Pharma | other, Inhibitor | Other, 20S Proteasome Inhibitor | 0 | |
| AM 114 | Pharma | 20S proteasome inhibitor | 0 | |||
| CEP-18770 | Pharma | Proteasome inhibitor | 0 | |||
| MLN9708 | Pharma | Proteasome inhibitor, 20S Proteasome Inhibitor | 0 |
(3) ApexBio Compounds for PSME3 Gene
Transcripts for PSME3 Gene
mRNA/cDNA for PSME3 Gene
- 4 REFSEQ mRNAs :
- 21 NCBI additional mRNA sequence :
- 17 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :
-
- ENST00000293362 3144 nts (view in UCSC)
- ENST00000441946 2944 nts (view in UCSC)
- ENST00000541124 3366 nts (view in UCSC)
- ENST00000543428 956 nts (view in UCSC)
- ENST00000545225 3082 nts (view in UCSC)
CRISPR Products
- Applied Biological Materials (abm): CRISPR Clones for PSME3 - Select products 50% OFF >
- * PSME3 CRISPR as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
-
OriGene CRISPR knockouts for PSME3
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for PSME3
-
Santa Cruz Biotechnology (SCBT) CRISPR for PSME3
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for PSME3
- Browse OriGene Inhibitory RNA Products For PSME3
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PSME3
Clone Products
- Applied Biological Materials (abm): Clones for PSME3 - Select products 50% OFF >
- * PSME3 as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
-
- MG203255
- MR203255
- MR203255L3
- MR203255L4
- RC200308
- RC200308L1
- RC200308L2
- RC200308L3
- RC200308L4
- RC222312
- RC222312L1
- RC222312L2
- RC222312L3
- RC222312L4
- RC232329
- RG200308
- RG222312
- RG232329
- RR215566
- RR215566L3
- RR215566L4
- MR203255L3V
- MR203255L4V
- RC200308L1V
- RC200308L2V
- RC200308L3V
- RC200308L4V
- RC222312L1V
- RC222312L2V