The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 n... See more...

Aliases for PSMD4 Gene

Aliases for PSMD4 Gene

  • Proteasome 26S Subunit, Non-ATPase 4 2 3 5
  • Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4 2 3
  • 26S Proteasome Non-ATPase Regulatory Subunit 4 3 4
  • 26S Proteasome Regulatory Subunit S5A 3 4
  • Multiubiquitin Chain-Binding Protein 3 4
  • Antisecretory Factor 1 3 4
  • MCB1 3 4
  • ASF 3 4
  • AF 3 4
  • 26S Proteasome Regulatory Subunit RPN10 4
  • S5a/Antisecretory Factor Protein 3
  • Angiocidin 3
  • PUB-R5 3
  • Rpn10 3
  • AF-1 3
  • S5A 3

External Ids for PSMD4 Gene

Previous GeneCards Identifiers for PSMD4 Gene

  • GC01P149069
  • GC01P146974
  • GC01P148002
  • GC01P148443
  • GC01P148040
  • GC01P149493
  • GC01P122605

Summaries for PSMD4 Gene

Entrez Gene Summary for PSMD4 Gene

  • The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq, Jul 2008]

CIViC Summary for PSMD4 Gene

GeneCards Summary for PSMD4 Gene

PSMD4 (Proteasome 26S Subunit, Non-ATPase 4) is a Protein Coding gene. Diseases associated with PSMD4 include Cholera and Cystic Fibrosis. Among its related pathways are Metabolism of proteins and RET signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding.

UniProtKB/Swiss-Prot Summary for PSMD4 Gene

  • Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.

Gene Wiki entry for PSMD4 Gene

Additional gene information for PSMD4 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for PSMD4 Gene

Genomics for PSMD4 Gene

GeneHancer (GH) Regulatory Elements for PSMD4 Gene

Promoters and enhancers for PSMD4 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around PSMD4 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for PSMD4

Top Transcription factor binding sites by QIAGEN in the PSMD4 gene promoter:
  • c-Myc
  • CUTL1
  • FOXD3
  • LCR-F1
  • Max1
  • Nkx2-2
  • POU2F1
  • POU2F1a
  • STAT3

Genomic Locations for PSMD4 Gene

Genomic Locations for PSMD4 Gene
chr1:151,254,700-151,267,479
(GRCh38/hg38)
Size:
12,780 bases
Orientation:
Plus strand
chr1:151,227,179-151,239,955
(GRCh37/hg19)
Size:
12,777 bases
Orientation:
Plus strand

Genomic View for PSMD4 Gene

Genes around PSMD4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PSMD4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PSMD4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PSMD4 Gene

Proteins for PSMD4 Gene

  • Protein details for PSMD4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P55036-PSMD4_HUMAN
    Recommended name:
    26S proteasome non-ATPase regulatory subunit 4
    Protein Accession:
    P55036
    Secondary Accessions:
    • D3DV16
    • Q5VWC5
    • Q9NS92

    Protein attributes for PSMD4 Gene

    Size:
    377 amino acids
    Molecular mass:
    40737 Da
    Quaternary structure:
    • Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases and few additional components including PSMD4 (PubMed:27428775, PubMed:27342858). Interacts with NUB1 (PubMed:11585840). Interacts with SQSTM1 (PubMed:15340068). Interacts with UBQLN4 (PubMed:15280365). Interacts with UBE3A (PubMed:22645313). Interacts with UBQLN1 (via ubiquitin-like domain) (PubMed:15147878). Interacts with DDI2 (PubMed:29290612).
    Miscellaneous:
    • [Isoform Rpn10E]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

    Three dimensional structures from OCA and Proteopedia for PSMD4 Gene

    Alternative splice isoforms for PSMD4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PSMD4 Gene

Post-translational modifications for PSMD4 Gene

  • Ubiquitination at Lys40, Lys83, Lys122, Lys126, Lys262, and Lys365
  • Modification sites at PhosphoSitePlus

Other Protein References for PSMD4 Gene

No data available for DME Specific Peptides for PSMD4 Gene

Domains & Families for PSMD4 Gene

Gene Families for PSMD4 Gene

HGNC:
Human Protein Atlas (HPA):
  • Cancer-related genes
  • Predicted intracellular proteins

Protein Domains for PSMD4 Gene

Blocks:
  • Ubiquitin interacting motif
InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for PSMD4 Gene

GenScript: Design optimal peptide antigens:
  • Proteasome (Prosome, macropain) 26S subunit, non-ATPase, 4 (A6PVV6_HUMAN)
  • Proteasome (Prosome, macropain) 26S subunit, non-ATPase, 4 (A6PVX4_HUMAN)
  • Multiubiquitin chain-binding protein (PSMD4_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P55036

UniProtKB/Swiss-Prot:

PSMD4_HUMAN :
  • The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
  • Belongs to the proteasome subunit S5A family.
Domain:
  • The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
Family:
  • Belongs to the proteasome subunit S5A family.
genes like me logo Genes that share domains with PSMD4: view

Function for PSMD4 Gene

Molecular function for PSMD4 Gene

UniProtKB/Swiss-Prot Function:
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.
GENATLAS Biochemistry:
multicatalytic proteinase complex (prosome,macropain 26S) regulator of proteasome,non ATPase subunit,50kDa,more acidic subunit 5A,specifically binding ubiquitin-lysozyme conjugates antisecretory factor 1,inhibiting intestinal fluid secretion

Phenotypes From GWAS Catalog for PSMD4 Gene

Gene Ontology (GO) - Molecular Function for PSMD4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003723 RNA binding HDA 22658674
GO:0005515 protein binding IPI 10488153
GO:0008134 transcription factor binding IEA --
GO:0031593 polyubiquitin modification-dependent protein binding IBA 21873635
GO:0042802 identical protein binding IPI 16990800
genes like me logo Genes that share ontologies with PSMD4: view
genes like me logo Genes that share phenotypes with PSMD4: view

Animal Models for PSMD4 Gene

MGI Knock Outs for PSMD4:

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PSMD4

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PSMD4 Gene

Localization for PSMD4 Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PSMD4 gene
Compartment Confidence
nucleus 5
cytosol 5
cytoskeleton 4
plasma membrane 3
extracellular 3
mitochondrion 3
endoplasmic reticulum 3
endosome 3
lysosome 3
peroxisome 2
golgi apparatus 2

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nucleoplasm (4)
  • Cytosol (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for PSMD4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000502 proteasome complex IEA,IDA 17323924
GO:0005634 nucleus IBA 21873635
GO:0005654 nucleoplasm IDA,TAS --
GO:0005737 cytoplasm IEA --
GO:0005829 cytosol TAS,IDA --
genes like me logo Genes that share ontologies with PSMD4: view

No data available for Subcellular locations from UniProtKB/Swiss-Prot for PSMD4 Gene

Pathways & Interactions for PSMD4 Gene

PathCards logo

SuperPathways for PSMD4 Gene

SuperPathway Contained pathways
1 CDK-mediated phosphorylation and removal of Cdc6
.78
.31
2 RET signaling
3 Chks in Checkpoint Regulation
.59
.59
.55
.43
.32
.31
4 HIV Life Cycle
.45
5 Class I MHC mediated antigen processing and presentation
genes like me logo Genes that share pathways with PSMD4: view

Pathways by source for PSMD4 Gene

2 KEGG pathways for PSMD4 Gene
3 GeneGo (Thomson Reuters) pathways for PSMD4 Gene
  • Immune response Antigen presentation by MHC class I
  • Proteolysis Role of Parkin in the Ubiquitin-Proteasomal Pathway
  • Transcription Ligand-Dependent Transcription of Retinoid-Target genes
19 Qiagen pathways for PSMD4 Gene
  • CDK5 Pathway
  • Chks in Checkpoint Regulation
  • DNA Repair Mechanisms
  • Estrogen-mediated S-Phase Entry
  • G2-M Phase Transition

Gene Ontology (GO) - Biological Process for PSMD4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000165 MAPK cascade TAS --
GO:0000209 protein polyubiquitination TAS --
GO:0002223 stimulatory C-type lectin receptor signaling pathway TAS --
GO:0002479 antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent TAS --
GO:0006521 regulation of cellular amino acid metabolic process TAS --
genes like me logo Genes that share ontologies with PSMD4: view

No data available for SIGNOR curated interactions for PSMD4 Gene

Drugs & Compounds for PSMD4 Gene

(17) Drugs for PSMD4 Gene - From: ApexBio, DGIdb, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Bortezomib Approved, Investigational Pharma inhibitor, other Proteosome and NF-kappaB inhibitor, Other, Proteasome inhibitors 888
carfilzomib Approved, Investigational Pharma other, Inhibitor Other, 20S Proteasome Inhibitor 0
AM 114 Pharma 20S proteasome inhibitor 0
Bortezomib (PS-341) Pharma Proteasome Inhibitor 0
Carfilzomib (PR-171) Pharma Proteasome inhibitor,epoxomicin analog 0

(1) Additional Compounds for PSMD4 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs

(14) ApexBio Compounds for PSMD4 Gene

Compound Action Cas Number
AM 114 20S proteasome inhibitor 856849-35-9
Bortezomib (PS-341) Proteasome Inhibitor 179324-69-7
Carfilzomib (PR-171) Proteasome inhibitor,epoxomicin analog 868540-17-4
Celastrol Antioxidant, anti-inflammatory and immunosuppressive agent 34157-83-0
CEP-18770 Proteasome inhibitor 847499-27-8
Dihydroeponemycin Proteasome inhibitor,antitumor reagent,eponemycin ddrivative 126463-64-7
Epoxomicin Proteasome inhibitor 134381-21-8
MG-132 Proteasome inhibitor, Cell permeable, reversible 133407-82-6
MLN2238 β5 site of the 20S proteasome inhibitor 1072833-77-2
MLN9708 Proteasome inhibitor 1201902-80-8
ONX-0914 (PR-957) Immunoproteasome inhibitor,potent and selective 960374-59-8
Oprozomib (ONX-0912) Proteasome inhibitor 935888-69-0
PI-1840 Chymotrypsin-like (CT-L) inhibitor 1401223-22-0
PSI Proteasome inhibitor 158442-41-2
genes like me logo Genes that share compounds with PSMD4: view

Drug Products

Transcripts for PSMD4 Gene

mRNA/cDNA for PSMD4 Gene

3 REFSEQ mRNAs :
5 NCBI additional mRNA sequence :
12 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PSMD4

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for PSMD4 Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4a · 4b ^ 5a · 5b ^ 6 ^ 7a · 7b · 7c ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b · 10c
SP1: - - -
SP2: - -
SP3:
SP4: -
SP5: -
SP6: -
SP7:

Relevant External Links for PSMD4 Gene

GeneLoc Exon Structure for
PSMD4

Expression for PSMD4 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for PSMD4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for PSMD4 Gene

This gene is overexpressed in Amniocyte (6.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for PSMD4 Gene