The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiq... See more...

Aliases for PSMB8 Gene

Aliases for PSMB8 Gene

  • Proteasome 20S Subunit Beta 8 2 3
  • Proteasome Subunit Beta 8 2 3 5
  • Multicatalytic Endopeptidase Complex Subunit C13 3 4
  • Really Interesting New Gene 10 Protein 3 4
  • Proteasome Subunit Beta Type-8 3 4
  • Low Molecular Mass Protein 7 3 4
  • Proteasome Component C13 3 4
  • Macropain Subunit C13 3 4
  • EC 3.4.25.1 4 52
  • PSMB5i 3 4
  • RING10 3 4
  • LMP7 3 4
  • Proteasome (Prosome, Macropain) Subunit, Beta Type, 8 (Large Multifunctional Peptidase 7) 3
  • Proteasome (Prosome, Macropain) Subunit, Beta Type, 8 (Large Multifunctional Protease 7) 2
  • Proteasome (Prosome, Macropain) Subunit, Beta Type, 8 2
  • Large Multifunctional Peptidase 7 2
  • Proteasome Catalytic Subunit 3i 3
  • Low Molecular Weight Protein 7 3
  • Proteasome Subunit Beta 5i 3
  • Proteasome Subunit Beta-5i 4
  • Proteasome-Related Gene 7 3
  • Protease Component C13 3
  • Proteasome Subunit Β5i 2
  • Proteasome Subunit Y2 3
  • D6S216E 3
  • D6S216 3
  • PRAAS1 3
  • ALDD 3
  • NKJO 3
  • JMP 3
  • Y2 4

External Ids for PSMB8 Gene

Previous HGNC Symbols for PSMB8 Gene

  • LMP7

Previous GeneCards Identifiers for PSMB8 Gene

  • GC06M032805
  • GC06M032579
  • GC06M032855
  • GC06M032916
  • GC06M032808

Summaries for PSMB8 Gene

Entrez Gene Summary for PSMB8 Gene

  • The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]

CIViC Summary for PSMB8 Gene

GeneCards Summary for PSMB8 Gene

PSMB8 (Proteasome 20S Subunit Beta 8) is a Protein Coding gene. Diseases associated with PSMB8 include Proteasome-Associated Autoinflammatory Syndrome 1 and Nakajo Syndrome. Among its related pathways are Metabolism of proteins and RET signaling. Gene Ontology (GO) annotations related to this gene include threonine-type endopeptidase activity. An important paralog of this gene is PSMB5.

UniProtKB/Swiss-Prot Summary for PSMB8 Gene

  • The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein (PubMed:27049119). Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.

Gene Wiki entry for PSMB8 Gene

Additional gene information for PSMB8 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for PSMB8 Gene

Genomics for PSMB8 Gene

GeneHancer (GH) Regulatory Elements for PSMB8 Gene

Promoters and enhancers for PSMB8 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around PSMB8 on UCSC Golden Path with GeneCards custom track

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for PSMB8

Top Transcription factor binding sites by QIAGEN in the PSMB8 gene promoter:
  • STAT3

Genomic Locations for PSMB8 Gene

Genomic Locations for PSMB8 Gene
chr6:32,840,717-32,844,935
(GRCh38/hg38)
Size:
4,219 bases
Orientation:
Minus strand
chr6:32,808,494-32,812,712
(GRCh37/hg19)
Size:
4,219 bases
Orientation:
Minus strand

Genomic View for PSMB8 Gene

Genes around PSMB8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PSMB8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PSMB8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

Proteins for PSMB8 Gene

  • Protein details for PSMB8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P28062-PSB8_HUMAN
    Recommended name:
    Proteasome subunit beta type-8
    Protein Accession:
    P28062
    Secondary Accessions:
    • B0UZC0
    • Q29824
    • Q5JNW6
    • Q5QNR8
    • Q96J48

    Protein attributes for PSMB8 Gene

    Size:
    276 amino acids
    Molecular mass:
    30354 Da
    Quaternary structure:
    • The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP. Interacts with TAP1.
    • (Microbial infection) Interacts with HIV-1 TAT protein.

    Three dimensional structures from OCA and Proteopedia for PSMB8 Gene

    Alternative splice isoforms for PSMB8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PSMB8 Gene

Selected DME Specific Peptides for PSMB8 Gene

P28062:
  • HGTTTLAF
  • GPGLYYVD
  • ISVSAASK
  • KVIEINP

Post-translational modifications for PSMB8 Gene

  • Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
  • Ubiquitination at Lys153, Lys179, and Lys252
  • Modification sites at PhosphoSitePlus

Other Protein References for PSMB8 Gene

Domains & Families for PSMB8 Gene

Gene Families for PSMB8 Gene

HGNC:
Human Protein Atlas (HPA):
  • Disease related genes
  • Enzymes
  • FDA approved drug targets
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for PSMB8 Gene

Suggested Antigen Peptide Sequences for PSMB8 Gene

GenScript: Design optimal peptide antigens:
  • Really interesting new gene 10 protein (PSB8_HUMAN)
  • PSMB8 protein (Q6FHU0_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P28062

UniProtKB/Swiss-Prot:

PSB8_HUMAN :
  • Belongs to the peptidase T1B family.
Family:
  • Belongs to the peptidase T1B family.
genes like me logo Genes that share domains with PSMB8: view

Function for PSMB8 Gene

Molecular function for PSMB8 Gene

UniProtKB/Swiss-Prot Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein (PubMed:27049119). Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=Cleavage of peptide bonds with very broad specificity.; EC=3.4.25.1;.
UniProtKB/Swiss-Prot Induction:
Up-regulated by IFNG/IFN-gamma and IRF1 (at protein level). Up-regulated by TNF (at protein level). Up-regulated by tetrodotoxin (TTX) in glial cells. Up-regulated in Crohn's bowel disease (CD). Down-regulated by the selective inhibitor PR-957. Down-regulated in mature dendritic cells by HSV-1 infection. Up-regulated by heat shock treatment.
GENATLAS Biochemistry:
multicatalytic proteinase complex (20S) proteasome (prosome,macropain) component 7,beta-type subunit,stimulated by interferon gamma,involved in the degradation of cytoplasmic antigens for MHC class I antigen presentation pathways

Enzyme Numbers (IUBMB) for PSMB8 Gene

Phenotypes From GWAS Catalog for PSMB8 Gene

Gene Ontology (GO) - Molecular Function for PSMB8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004175 endopeptidase activity IBA 21873635
GO:0004298 threonine-type endopeptidase activity IEA --
GO:0005515 protein binding IPI 15303969
GO:0008233 peptidase activity IEA --
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with PSMB8: view
genes like me logo Genes that share phenotypes with PSMB8: view

Human Phenotype Ontology for PSMB8 Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for PSMB8 Gene

MGI Knock Outs for PSMB8:

Animal Model Products

CRISPR Products

miRNA for PSMB8 Gene

miRTarBase miRNAs that target PSMB8

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PSMB8

Clone Products

  • Addgene plasmids for PSMB8

No data available for Transcription Factor Targets and HOMER Transcription for PSMB8 Gene

Localization for PSMB8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PSMB8 Gene

Cytoplasm. Nucleus.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PSMB8 gene
Compartment Confidence
nucleus 5
cytosol 5
extracellular 3
plasma membrane 2
cytoskeleton 2
mitochondrion 2
endoplasmic reticulum 2
endosome 2
lysosome 2
golgi apparatus 2
peroxisome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytokinetic bridge (2)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for PSMB8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000502 proteasome complex IDA,TAS 26524591
GO:0005634 nucleus IBA 21873635
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IBA 21873635
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with PSMB8: view

Pathways & Interactions for PSMB8 Gene

PathCards logo

SuperPathways for PSMB8 Gene

SuperPathway Contained pathways
1 CDK-mediated phosphorylation and removal of Cdc6
.78
.31
2 RET signaling
3 Chks in Checkpoint Regulation
.59
.59
.55
.43
.32
4 HIV Life Cycle
.45
5 Class I MHC mediated antigen processing and presentation
genes like me logo Genes that share pathways with PSMB8: view

Pathways by source for PSMB8 Gene

1 GeneGo (Thomson Reuters) pathway for PSMB8 Gene
  • Immune response Antigen presentation by MHC class I
12 Qiagen pathways for PSMB8 Gene
  • CDK5 Pathway
  • Chks in Checkpoint Regulation
  • DNA Repair Mechanisms
  • Estrogen-mediated S-Phase Entry
  • G2-M Phase Transition
1 Cell Signaling Technology pathway for PSMB8 Gene

Gene Ontology (GO) - Biological Process for PSMB8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000165 MAPK cascade TAS --
GO:0000209 protein polyubiquitination TAS --
GO:0002223 stimulatory C-type lectin receptor signaling pathway TAS --
GO:0002376 immune system process IEA --
GO:0002479 antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent TAS --
genes like me logo Genes that share ontologies with PSMB8: view

No data available for SIGNOR curated interactions for PSMB8 Gene

Drugs & Compounds for PSMB8 Gene