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The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiq... See more...
Aliases for PSMB8 Gene
Aliases for PSMB8 Gene
- Proteasome 20S Subunit Beta 8 2 3 5
- PSMB5i 2 3 4
- RING10 2 3 4
- Multicatalytic Endopeptidase Complex Subunit C13 3 4
- Really Interesting New Gene 10 Protein 3 4
- Proteasome Subunit Beta Type-8 3 4
- Low Molecular Mass Protein 7 3 4
- Proteasome Subunit Beta 8 2 3
- Proteasome Component C13 3 4
- Macropain Subunit C13 3 4
- EC 3.4.25.1 4 51
- D6S216E 2 3
- LMP7 3 4
- Proteasome (Prosome, Macropain) Subunit, Beta Type, 8 (Large Multifunctional Peptidase 7) 3
- Proteasome (Prosome, Macropain) Subunit, Beta Type, 8 (Large Multifunctional Protease 7) 2
- Proteasome (Prosome, Macropain) Subunit, Beta Type, 8 2
- Large Multifunctional Peptidase 7 2
- Proteasome Catalytic Subunit 3i 3
- Low Molecular Weight Protein 7 3
- Proteasome Subunit Beta 5i 3
- Proteasome Subunit Beta-5i 4
- Proteasome-Related Gene 7 3
- Proteasome Subunit Β5i 2
- Protease Component C13 3
- Proteasome Subunit Y2 3
- D6S216 3
- PRAAS1 3
- Beta5i 2
- PSMB8 5
- ALDD 3
- NKJO 3
- JMP 3
- Y2 4
External Ids for PSMB8 Gene
- HGNC: 9545
- Entrez Gene: 5696
- Ensembl: ENSG00000204264
- OMIM: 177046
- UniProtKB: P28062
Previous HGNC Symbols for PSMB8 Gene
- LMP7
Previous GeneCards Identifiers for PSMB8 Gene
- GC06M032805
- GC06M032579
- GC06M032855
- GC06M032916
- GC06M032808
Summaries for PSMB8 Gene
-
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]
GeneCards Summary for PSMB8 Gene
PSMB8 (Proteasome 20S Subunit Beta 8) is a Protein Coding gene. Diseases associated with PSMB8 include Proteasome-Associated Autoinflammatory Syndrome 1 and Microcytic Anemia. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Antigen processing-Cross presentation. Gene Ontology (GO) annotations related to this gene include threonine-type endopeptidase activity. An important paralog of this gene is PSMB5.
UniProtKB/Swiss-Prot Summary for PSMB8 Gene
-
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein (PubMed:27049119). Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
Additional gene information for PSMB8 Gene
- HGNC(9545)
- Entrez Gene(5696)
- Ensembl(ENSG00000204264)
- OMIM(177046)
- UniProtKB(P28062)
- Open Targets Platform(ENSG00000204264)
- Monarch Initiative
- Search for PSMB8 at DataMed
- Search for PSMB8 at HumanCyc
No data available for Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for PSMB8 Gene
Genomics for PSMB8 Gene
GeneHancer (GH) Regulatory Elements Pubs
Promoters and enhancers for PSMB8 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication |
Request up-to-date GeneHancer data (full dataset)
GeneHancers around PSMB8 on the GeneHancer Hub at the UCSC Golden Path
Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for PSMB8
- Top Transcription factor binding sites by QIAGEN in the PSMB8 gene promoter:
-
- STAT3
Genomic Locations for PSMB8 Gene
Genomic Locations for PSMB8 Gene
- chr6:32,840,717-32,844,679
- (GRCh38/hg38)
- Size:
- 3,963 bases
- Orientation:
- Minus strand
- chr6:32,808,494-32,812,712
- (GRCh37/hg19)
- Size:
- 4,219 bases
- Orientation:
- Minus strand
Genomic View for PSMB8 Gene
Genes around PSMB8 on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 6p21.32 by HGNC
- 6p21.32 by Entrez Gene
- 6p21.32 by Ensembl
PSMB8 Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for PSMB8 Gene
Proteins for PSMB8 Gene
-
Protein details for PSMB8 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- P28062-PSB8_HUMAN
- Recommended name:
- Proteasome subunit beta type-8
- Protein Accession:
- P28062
- B0UZC0
- Q29824
- Q5JNW6
- Q5QNR8
- Q96J48
Protein attributes for PSMB8 Gene
- Size:
- 276 amino acids
- Molecular mass:
- 30354 Da
- Quaternary structure:
-
- The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP. Interacts with TAP1.
- (Microbial infection) Interacts with HIV-1 TAT protein.
Three dimensional structures from OCA and Proteopedia for PSMB8 Gene
Protein Expression for PSMB8 Gene
Selected DME Specific Peptides for PSMB8 Gene
- P28062:
-
- HGTTTLAF
- GPGLYYVD
- ISVSAASK
- KVIEINP
Post-translational modifications for PSMB8 Gene
- Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
-
Ubiquitination at Lys153, Lys179, and Lys252
- NX_P28062 (NX_P28062-1)
- Modification sites at PhosphoSitePlus
Other Protein References for PSMB8 Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibody Products
- LifeSpan BioSciences PSMB8 / LMP7 Antibody
- GeneTex Anti-PSMB8 antibody [1A5] and Anti-PSMB8 antibody [N2C3]
-
Custom Antibody ServicesOriGene Antibodies for PSMB8
- Novus Biologicals Antibodies for PSMB8
-
Abcam antibodies for PSMB8
- Invitrogen Antibodies for PSMB8 (AFLGC-Psmb8)
-
Santa Cruz Biotechnology (SCBT) Antibodies for PSMB8
- Sino Biological Antibodies for PSMB8
Protein Products
- Search Origene for MassSpec and Protein Over-expression Lysates for PSMB8
- Origene Custom Protein Services for PSMB8
- ProSpec Recombinant Proteins for PSMB8
-
Abcam proteins for PSMB8
Assay Products
- G Biosciences Immunotag™ Proteasome subunit beta type-8 ELISA Kit
- RayBiotech Human LMP7/PSMB8 ELISA
Domains & Families for PSMB8 Gene
Gene Families for PSMB8 Gene
- HGNC:
- Human Protein Atlas (HPA):
-
- Disease related genes
- Enzymes
- FDA approved drug targets
- Plasma proteins
- Predicted intracellular proteins
Protein Domains for PSMB8 Gene
- InterPro:
- Blocks:
-
- Proteasome B-type subunit
- ProtoNet:
Suggested Antigen Peptide Sequences for PSMB8 Gene
- GenScript: Design optimal peptide antigens:
-
- Really interesting new gene 10 protein (PSB8_HUMAN)
- PSMB8 protein (Q6FHU0_HUMAN)
Graphical View of Domain Structure for InterPro Entry
P28062- Family:
-
- Belongs to the peptidase T1B family.
Function for PSMB8 Gene
Molecular function for PSMB8 Gene
- UniProtKB/Swiss-Prot Function:
- The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein (PubMed:27049119). Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
- UniProtKB/Swiss-Prot CatalyticActivity:
- Reaction=Cleavage of peptide bonds with very broad specificity.; EC=3.4.25.1;.
- UniProtKB/Swiss-Prot Induction:
- Up-regulated by IFNG/IFN-gamma and IRF1 (at protein level). Up-regulated by TNF (at protein level). Up-regulated by tetrodotoxin (TTX) in glial cells. Up-regulated in Crohn's bowel disease (CD). Down-regulated by the selective inhibitor PR-957. Down-regulated in mature dendritic cells by HSV-1 infection. Up-regulated by heat shock treatment.
- GENATLAS Biochemistry:
- multicatalytic proteinase complex (20S) proteasome (prosome,macropain) component 7,beta-type subunit,stimulated by interferon gamma,involved in the degradation of cytoplasmic antigens for MHC class I antigen presentation pathways
Enzyme Numbers (IUBMB) for PSMB8 Gene
Phenotypes From GWAS Catalog for PSMB8 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0004175 | endopeptidase activity | IBA | 21873635 |
| GO:0004298 | threonine-type endopeptidase activity | IEA | -- |
| GO:0005515 | protein binding | IPI | 15303969 |
| GO:0008233 | peptidase activity | IEA | -- |
| GO:0016787 | hydrolase activity | IEA | -- |
Phenotypes for PSMB8 Gene
- MGI mutant phenotypes for PSMB8:
- inferred from 2 alleles
- GenomeRNAi human phenotypes for PSMB8:
-
- Large nuclei
- no effect
- Increased cell death HMECs cells
- Decreased viability
- Increased epidermal growth factor (EGF) endocytosis
- Decreased Salmonella enterica Typhimurium invasion
- Negative genetic interaction between KRASG13D/+ and KRAS+/-
- shRNA abundance <= 25%
- Synthetic lethal with MLN4924 (a NAE inhibitor)
- Increased viability with MLN4924 (a NAE inhibitor)
- Decreased interferon-beta reporter expression
- Decreased viability in non-small-cell lung cancer (NSCLC) lineage; decreased viability in pancreas lineage
Animal Models for PSMB8 Gene
- MGI Knock Outs for PSMB8:
-
- Psmb8 Psmb8<tm1Hjf>
- Psmb9/Psmb8 Psmb9/Psmb8<tm1Klr>
Animal Model Products
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
CRISPR Products
- Applied Biological Materials (abm): CRISPR Clones for PSMB8 - Select products 50% OFF >
- * PSMB8 CRISPR as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
-
OriGene CRISPR knockouts for PSMB8
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for PSMB8
-
Santa Cruz Biotechnology (SCBT) CRISPR for PSMB8
miRNA for PSMB8 Gene
- miRTarBase miRNAs that target PSMB8
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for PSMB8
- Browse OriGene Inhibitory RNA Products For PSMB8
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PSMB8
Clone Products
- Applied Biological Materials (abm): Clones for PSMB8 - Select products 50% OFF >
- * PSMB8 as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
- Sino Biological Human cDNA Clone for PSMB8
- Addgene plasmids for PSMB8
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for PSMB8
No data available for Transcription Factor Targets and HOMER Transcription for PSMB8 Gene
Localization for PSMB8 Gene
Subcellular locations from UniProtKB/Swiss-Prot for PSMB8 Gene
- Cytoplasm. Nucleus.
| Compartment | Confidence |
|---|---|
| extracellular | 5 |
| nucleus | 5 |
| cytosol | 5 |
| plasma membrane | 2 |
| cytoskeleton | 2 |
| mitochondrion | 2 |
| endoplasmic reticulum | 2 |
| endosome | 2 |
| lysosome | 2 |
| golgi apparatus | 2 |
| peroxisome | 1 |
- Cytokinetic bridge (2)
- Vesicles (2)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000502 | proteasome complex | TAS,IDA | 26524591 |
| GO:0005634 | nucleus | IBA | 21873635 |
| GO:0005654 | nucleoplasm | TAS | -- |
| GO:0005737 | cytoplasm | IBA | 21873635 |
| GO:0005829 | cytosol | TAS | -- |
Pathways & Interactions for PSMB8 Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Regulation of activated PAK-2p34 by proteasome mediated degradation |
.93
.93
|
.80
.79
.78
.69
.47
|
| 2 | RET signaling |
.92
|
.92
|
| 3 | Chks in Checkpoint Regulation |
Chks in Checkpoint Regulation
.58
G2-M Phase Transition
.58
Estrogen-mediated S-Phase Entry
.54
|
SREBP Proteolysis
.42
DNA Repair Mechanisms
.31
|
| 4 | HIV Life Cycle |
.65
|
.45
|
| 5 | Class I MHC mediated antigen processing and presentation | ||
Pathways by source for PSMB8 Gene
1 Sino Biological pathway for PSMB8 Gene
1 BioSystems pathway for PSMB8 Gene
166 Reactome pathways for PSMB8 Gene
1 KEGG pathway for PSMB8 Gene
1 GeneGo (Thomson Reuters) pathway for PSMB8 Gene
- Immune response Antigen presentation by MHC class I
12 Qiagen pathways for PSMB8 Gene
- CDK5 Pathway
- Chks in Checkpoint Regulation
- DNA Repair Mechanisms
- Estrogen-mediated S-Phase Entry
- G2-M Phase Transition
1 Cell Signaling Technology pathway for PSMB8 Gene
Interacting Proteins for PSMB8 Gene
Selected Interacting proteins:
P28062-PSB8_HUMAN
ENSP00000364016
for PSMB8 Gene via
UniProtKB
STRING
IID
GPS-Prot Interaction Network for PSMB8
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000165 | MAPK cascade | TAS | -- |
| GO:0000209 | protein polyubiquitination | TAS | -- |
| GO:0002223 | stimulatory C-type lectin receptor signaling pathway | TAS | -- |
| GO:0002376 | immune system process | IEA | -- |
| GO:0002479 | antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent | TAS | -- |
No data available for SIGNOR curated interactions for PSMB8 Gene
Drugs & Compounds for PSMB8 Gene
| Name | Status | Disease Links | Group | Role | Mechanism of Action | Clinical Trials |
|---|---|---|---|---|---|---|
| carfilzomib | Approved, Investigational | Pharma | Target, inhibitor, other | Other, 20S Proteasome Inhibitor | 0 | |
| Bortezomib | Approved, Investigational | Pharma | inhibitor, other | Proteosome and NF-kappaB inhibitor, Other, Proteasome inhibitors | 910 | |
| AM 114 | Pharma | 20S proteasome inhibitor | 0 | |||
| Bortezomib (PS-341) | Pharma | Proteasome Inhibitor | 0 | |||
| Carfilzomib (PR-171) | Pharma | Proteasome inhibitor,epoxomicin analog | 0 |
(14) ApexBio Compounds for PSMB8 Gene
| Compound | Action | Cas Number |
|---|---|---|
| AM 114 | 20S proteasome inhibitor | 856849-35-9 |
| Bortezomib (PS-341) | Proteasome Inhibitor | 179324-69-7 |
| Carfilzomib (PR-171) | Proteasome inhibitor,epoxomicin analog | 868540-17-4 |
| Celastrol | Antioxidant, anti-inflammatory and immunosuppressive agent | 34157-83-0 |
| CEP-18770 | Proteasome inhibitor | 847499-27-8 |
| Dihydroeponemycin | Proteasome inhibitor,antitumor reagent,eponemycin ddrivative | 126463-64-7 |
| Epoxomicin | Proteasome inhibitor | 134381-21-8 |
| MG-132 | Proteasome inhibitor, Cell permeable, reversible | 133407-82-6 |
| MLN2238 | β5 site of the 20S proteasome inhibitor | 1072833-77-2 |
| MLN9708 | Proteasome inhibitor | 1201902-80-8 |
| ONX-0914 (PR-957) | Immunoproteasome inhibitor,potent and selective | 960374-59-8 |
| Oprozomib (ONX-0912) | Proteasome inhibitor | 935888-69-0 |
| PI-1840 | Chymotrypsin-like (CT-L) inhibitor | 1401223-22-0 |
| PSI | Proteasome inhibitor | 158442-41-2 |
Transcripts for PSMB8 Gene
mRNA/cDNA for PSMB8 Gene
- 2 REFSEQ mRNAs :
- 13 NCBI additional mRNA sequence :
- 7 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :
-
- ENST00000374881 1327 nts (view in UCSC)
- ENST00000374882 1124 nts (view in UCSC)
- ENST00000395339 1025 nts (view in UCSC)
- ENST00000484003 1153 nts (view in UCSC)
- ENST00000490613 835 nts (view in UCSC)