The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiq... See more...

Aliases for PSMB5 Gene

Aliases for PSMB5 Gene

  • Proteasome 20S Subunit Beta 5 2 3 5
  • Proteasome Subunit X 2 3 4
  • MB1 2 3 4
  • Proteasome (Prosome, Macropain) Subunit, Beta Type, 5 2 3
  • Multicatalytic Endopeptidase Complex Epsilon Chain 3 4
  • Proteasome Subunit Beta Type-5 3 4
  • Proteasome Subunit Beta 5 2 3
  • Proteasome Epsilon Chain 3 4
  • Macropain Epsilon Chain 3 4
  • Proteasome Subunit MB1 3 4
  • Proteasome Chain 6 3 4
  • EC 3.4.25.1 4 51
  • LMPX 3 4
  • X 3 4
  • PSX Large Multifunctional Protease X 3
  • Proteasome Subunit, Beta Type, 5 3
  • Testicular Tissue Protein Li 153 3
  • Proteasome Catalytic Subunit 3 3
  • Proteasome Beta 5 Subunit 3
  • PSMB5 5

External Ids for PSMB5 Gene

Previous GeneCards Identifiers for PSMB5 Gene

  • GC14M020867
  • GC14M017282
  • GC14M021475
  • GC14M021485
  • GC14M022564
  • GC14M023495
  • GC14M003612
  • GC14M023485

Summaries for PSMB5 Gene

Entrez Gene Summary for PSMB5 Gene

  • The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

GeneCards Summary for PSMB5 Gene

PSMB5 (Proteasome 20S Subunit Beta 5) is a Protein Coding gene. Diseases associated with PSMB5 include Cystic Fibrosis and Breast Cancer. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Antigen processing-Cross presentation. Gene Ontology (GO) annotations related to this gene include peptidase activity and threonine-type endopeptidase activity. An important paralog of this gene is PSMB8.

UniProtKB/Swiss-Prot Summary for PSMB5 Gene

  • Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.

Gene Wiki entry for PSMB5 Gene

Additional gene information for PSMB5 Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for PSMB5 Gene

Genomics for PSMB5 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for PSMB5 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around PSMB5 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for PSMB5

Top Transcription factor binding sites by QIAGEN in the PSMB5 gene promoter:
  • ATF
  • Nkx2-5
  • Pax-5
  • RFX1
  • Zic3

Genomic Locations for PSMB5 Gene

Genomic Locations for PSMB5 Gene
chr14:23,016,543-23,035,230
(GRCh38/hg38)
Size:
18,688 bases
Orientation:
Minus strand
chr14:23,485,752-23,504,439
(GRCh37/hg19)
Size:
18,688 bases
Orientation:
Minus strand

Genomic View for PSMB5 Gene

Genes around PSMB5 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PSMB5 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PSMB5 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PSMB5 Gene

Proteins for PSMB5 Gene

  • Protein details for PSMB5 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P28074-PSB5_HUMAN
    Recommended name:
    Proteasome subunit beta type-5
    Protein Accession:
    P28074
    Secondary Accessions:
    • B2R4N9
    • B4DUM9
    • D3DS43
    • E9PAV2
    • Q16242
    • Q6PEW2
    • Q7Z3B5
    • Q86T01
    • Q9TNN9

    Protein attributes for PSMB5 Gene

    Size:
    263 amino acids
    Molecular mass:
    28480 Da
    Quaternary structure:
    • The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Directly interacts with POMP (PubMed:15944226). Interacts with ABCB1 and TAP1 (PubMed:15488952).
    • (Microbial infection) Interacts with HIV-1 TAT protein.
    SequenceCaution:
    • Sequence=AAP35423.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAA06097.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=CAD97956.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for PSMB5 Gene

    Alternative splice isoforms for PSMB5 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PSMB5 Gene

Selected DME Specific Peptides for PSMB5 Gene

P28074:
  • GVMDRGYSYDL
  • RDAYSGG
  • HGTTTLAF
  • YELRNKE
  • ISVAAASK
  • GPGLYYVD
  • VAADSRA
  • KVIEINP
  • AGGAADC
  • DSRATAG

Post-translational modifications for PSMB5 Gene

  • Ubiquitination at Lys91 and Lys92
  • Modification sites at PhosphoSitePlus

Domains & Families for PSMB5 Gene

Gene Families for PSMB5 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • FDA approved drug targets
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for PSMB5 Gene

Suggested Antigen Peptide Sequences for PSMB5 Gene

GenScript: Design optimal peptide antigens:
  • Proteasome subunit X (PSB5_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P28074

UniProtKB/Swiss-Prot:

PSB5_HUMAN :
  • Belongs to the peptidase T1B family.
Family:
  • Belongs to the peptidase T1B family.
genes like me logo Genes that share domains with PSMB5: view

Function for PSMB5 Gene

Molecular function for PSMB5 Gene

UniProtKB/Swiss-Prot Function:
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=Cleavage of peptide bonds with very broad specificity.; EC=3.4.25.1; Evidence={ECO:0000269|PubMed:27176742};.
UniProtKB/Swiss-Prot Induction:
Down-regulated by IFNG/IFN-gamma (at protein level). Induced in breast cancer tissue. Up-regulated by sulforaphane in breast cancer cells.
GENATLAS Biochemistry:
multicatalytic proteinase complex (20S) proteasome (prosome,macropain) component,beta,subunit MB-1/X,22.9kDa

Enzyme Numbers (IUBMB) for PSMB5 Gene

Phenotypes From GWAS Catalog for PSMB5 Gene

Gene Ontology (GO) - Molecular Function for PSMB5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004175 endopeptidase activity IBA 21873635
GO:0004298 threonine-type endopeptidase activity IEA --
GO:0005515 protein binding IPI 14733938
GO:0008233 peptidase activity IDA 17540904
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with PSMB5: view
genes like me logo Genes that share phenotypes with PSMB5: view

Animal Model Products

  • Taconic Biosciences Mouse Models for PSMB5

CRISPR Products

miRNA for PSMB5 Gene

miRTarBase miRNAs that target PSMB5

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PSMB5

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for PSMB5 Gene

Localization for PSMB5 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PSMB5 Gene

Cytoplasm. Nucleus.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PSMB5 gene
Compartment Confidence
cytoskeleton 5
nucleus 5
cytosol 5
extracellular 4
mitochondrion 2
endoplasmic reticulum 2
plasma membrane 1
peroxisome 1
endosome 1
lysosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Centrosome (3)
  • Nucleoplasm (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for PSMB5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000502 proteasome complex TAS,IDA 17323924
GO:0005634 nucleus IBA 21873635
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IBA 21873635
GO:0005813 centrosome IDA --
genes like me logo Genes that share ontologies with PSMB5: view

Pathways & Interactions for PSMB5 Gene

PathCards logo

SuperPathways for PSMB5 Gene

SuperPathway Contained pathways
1 Regulation of activated PAK-2p34 by proteasome mediated degradation
.78
2 RET signaling
3 Chks in Checkpoint Regulation
.58
.58
.54
.42
.31
.30
4 HIV Life Cycle
.45
5 Parkinson disease
genes like me logo Genes that share pathways with PSMB5: view

Pathways by source for PSMB5 Gene

4 GeneGo (Thomson Reuters) pathways for PSMB5 Gene
  • Immune response Antigen presentation by MHC class I
  • Regulation of degradation of deltaF508 CFTR in CF
  • Regulation of degradation of wt-CFTR
  • Transcription Ligand-Dependent Transcription of Retinoid-Target genes
17 Qiagen pathways for PSMB5 Gene
  • CDK5 Pathway
  • Chks in Checkpoint Regulation
  • DNA Repair Mechanisms
  • Estrogen-mediated S-Phase Entry
  • G2-M Phase Transition

Gene Ontology (GO) - Biological Process for PSMB5 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000165 MAPK cascade TAS --
GO:0000209 protein polyubiquitination TAS --
GO:0002223 stimulatory C-type lectin receptor signaling pathway TAS --
GO:0002479 antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent TAS --
GO:0006508 proteolysis IDA 17540904
genes like me logo Genes that share ontologies with PSMB5: view

No data available for SIGNOR curated interactions for PSMB5 Gene

Drugs & Compounds for PSMB5 Gene

(19) Drugs for PSMB5 Gene - From: DrugBank, ApexBio, and DGIdb

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Bortezomib Approved, Investigational Pharma Target, inhibitor, other Proteosome and NF-kappaB inhibitor, Other, Proteasome inhibitors 910
carfilzomib Approved, Investigational Pharma Target, inhibitor, other Other, 20S Proteasome Inhibitor 0
(3AR,6R,6AS)-6-((S)-((S)-CYCLOHEX-2-ENYL)(HYDROXY)METHYL)-6A-METHYL-4-OXO-HEXAHYDRO-2H-FURO[3,2-C]PYRROLE-6-CARBALDEHYDE Experimental Pharma Target 0
Oprozomib Investigational Pharma 0
AM 114 Pharma 20S proteasome inhibitor 0

(14) ApexBio Compounds for PSMB5 Gene

Compound Action Cas Number
AM 114 20S proteasome inhibitor 856849-35-9
Bortezomib (PS-341) Proteasome Inhibitor 179324-69-7
Carfilzomib (PR-171) Proteasome inhibitor,epoxomicin analog 868540-17-4
Celastrol Antioxidant, anti-inflammatory and immunosuppressive agent 34157-83-0
CEP-18770 Proteasome inhibitor 847499-27-8
Dihydroeponemycin Proteasome inhibitor,antitumor reagent,eponemycin ddrivative 126463-64-7
Epoxomicin Proteasome inhibitor 134381-21-8
MG-132 Proteasome inhibitor, Cell permeable, reversible 133407-82-6
MLN2238 β5 site of the 20S proteasome inhibitor 1072833-77-2
MLN9708 Proteasome inhibitor 1201902-80-8
ONX-0914 (PR-957) Immunoproteasome inhibitor,potent and selective 960374-59-8
Oprozomib (ONX-0912) Proteasome inhibitor 935888-69-0
PI-1840 Chymotrypsin-like (CT-L) inhibitor 1401223-22-0
PSI Proteasome inhibitor 158442-41-2
genes like me logo Genes that share compounds with PSMB5: view

Transcripts for PSMB5 Gene

mRNA/cDNA for PSMB5 Gene

3 REFSEQ mRNAs :
13 NCBI additional mRNA sequence :
6 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PSMB5

Alternative Splicing Database (ASD) splice patterns (SP) for PSMB5 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b
SP1: - -
SP2: - - -
SP3: -
SP4:

Relevant External Links for PSMB5 Gene

GeneLoc Exon Structure for
PSMB5

Expression for PSMB5 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for PSMB5 Gene