Free for academic non-profit institutions. Other users need a Commercial license
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiq... See more...
Aliases for PSMB4 Gene
Aliases for PSMB4 Gene
- Proteasome 20S Subunit Beta 4 2 3 5
- PROS26 2 3 4
- Proteasome (Prosome, Macropain) Subunit, Beta Type, 4 2 3
- Multicatalytic Endopeptidase Complex Beta Chain 3 4
- Proteasome Subunit Beta Type-4 3 4
- Proteasome Subunit Beta 4 2 3
- 26 KDa Prosomal Protein 3 4
- Proteasome Beta Chain 3 4
- Macropain Beta Chain 3 4
- Proteasome Chain 3 3 4
- HsBPROS26 3 4
External Ids for PSMB4 Gene
- HGNC: 9541
- NCBI Entrez Gene: 5692
- Ensembl: ENSG00000159377
- OMIM®: 602177
- UniProtKB/Swiss-Prot: P28070
Previous GeneCards Identifiers for PSMB4 Gene
- GC01P149213
- GC01P147119
- GC01P148147
- GC01P148588
- GC01P148185
- GC01P149610
- GC01P122750
Summaries for PSMB4 Gene
-
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq, Jul 2008]
GeneCards Summary for PSMB4 Gene
PSMB4 (Proteasome 20S Subunit Beta 4) is a Protein Coding gene. Diseases associated with PSMB4 include Proteasome-Associated Autoinflammatory Syndrome 3 and Nakajo Syndrome. Among its related pathways are Downstream signaling events of B Cell Receptor (BCR) and CDK-mediated phosphorylation and removal of Cdc6. Gene Ontology (GO) annotations related to this gene include threonine-type endopeptidase activity and lipopolysaccharide binding. An important paralog of this gene is PSMB8.
UniProtKB/Swiss-Prot Summary for PSMB4 Gene
-
Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Additional gene information for PSMB4 Gene
- HGNC (9541)
- NCBI Entrez Gene (5692)
- Ensembl (ENSG00000159377)
- OMIM® (602177)
- UniProtKB/Swiss-Prot (P28070)
- Open Targets Platform(ENSG00000159377)
- Monarch Initiative
- Search for PSMB4 at DataMed
- Search for PSMB4 at HumanCyc
No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for PSMB4 Gene
Genomics for PSMB4 Gene
GeneHancer (GH) Regulatory Elements Pubs
Promoters and enhancers for PSMB4 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication |
Request up-to-date GeneHancer data (full dataset)
GeneHancers around PSMB4 on the GeneHancer Hub at the UCSC Golden Path
Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for PSMB4
- Top Transcription factor binding sites by QIAGEN in the PSMB4 gene promoter:
-
- Cart-1
- COMP1
- HNF-1
- HNF-1A
- HNF-4alpha1
- ISGF-3
- NF-1
- NF-1/L
- USF-1
- USF1
Genomic Locations for PSMB4 Gene
Latest Assembly
- chr1:151,399,560-151,401,937
- (GRCh38/hg38)
- Size:
- 2,378 bases
- Orientation:
- Plus strand
Previous Assembly
- chr1:151,372,049-151,374,413
- (GRCh37/hg19 by Entrez Gene)
- Size:
- 2,365 bases
- Orientation:
- Plus strand
- chr1:151,372,010-151,374,420
- (GRCh37/hg19 by Ensembl)
- Size:
- 2,411 bases
- Orientation:
- Plus strand
Genomic View for PSMB4 Gene
Genes around PSMB4 on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 1q21.3 by HGNC
- 1q21.3 by Entrez Gene
- 1q21.3 by Ensembl
PSMB4 Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for PSMB4 Gene
Proteins for PSMB4 Gene
-
Protein details for PSMB4 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- P28070-PSB4_HUMAN
- Recommended name:
- Proteasome subunit beta type-4
- Protein Accession:
- P28070
- B2R9L3
- P31148
- Q5SZS5
- Q6IBI4
- Q969L6
Protein attributes for PSMB4 Gene
- Size:
- 264 amino acids
- Molecular mass:
- 29204 Da
- Quaternary structure:
-
- The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Forms a ternary complex with SMAD1 and OAZ1 before PSMB4 is incorporated into the 20S proteasome. Interacts with PRPF19 (PubMed:11571290, PubMed:12097147).
- (Microbial infection) Interacts with HTLV-1 Tax protein.
- (Microbial infection) Interacts with HIV-1 Nef and Tat proteins.
Protein Expression for PSMB4 Gene
Selected DME Specific Peptides for PSMB4 Gene
- P28070:
-
- LGASGDYAD
- QMVIDEEL
- RRSKMNPLWN
- LGSYGSL
- AIHSWLTRAMY
- GVVIAAD
- VLYYRDARSYNRF
- GESFLGYVD
- PITRTQNPMVTGTSVLG
Post-translational modifications for PSMB4 Gene
Other Protein References for PSMB4 Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibodies for research
- LifeSpan BioSciences PSMB4 Monoclonal Antibody and PSMB4 Antibody
- G Biosciences Immunotag™ PSMB4 Antibody
-
Custom Antibody ServicesOriGene Antibodies for PSMB4
- Novus Biologicals Antibodies for PSMB4
-
Abcam antibodies for PSMB4
- Invitrogen Antibodies for PSMB4 (AFLGC-PSMB4)
-
Boster Bio Antibodies for PSMB4
-
Custom Antibody ServicesProSci Antibodies for PSMB4
- proteasome (prosome, macropain) subunit, beta type, 4 [19-802]
- proteasome (prosome, macropain) subunit, beta type, 4 [27-089]
- proteasome (prosome, macropain) subunit, beta type, 4 [42-648]
- proteasome (prosome, macropain) subunit, beta type, 4 [42-830]
- proteasome (prosome, macropain) subunit, beta type, 4 [XW-8085]
-
Santa Cruz Biotechnology (SCBT) Antibodies for PSMB4
Protein products for research
- Search Origene for MassSpec and Protein Over-expression Lysates for PSMB4
- Origene Custom Protein Services for PSMB4
- ProSpec Recombinant Proteins for PSMB4
-
Abcam proteins for PSMB4
Assay products for research
- antibodies-online: Show 12 available PSMB4 Elisa Kits ranked by validation data
- Compare Top PSMB4 Elisa Kits
-
Quality Products:
Domains & Families for PSMB4 Gene
Gene Families for PSMB4 Gene
- HGNC:
- Human Protein Atlas (HPA):
-
- Enzymes
- Plasma proteins
- Predicted intracellular proteins
Protein Domains for PSMB4 Gene
- InterPro:
- Blocks:
-
- Proteasome B-type subunit
- ProtoNet:
Suggested Antigen Peptide Sequences for PSMB4 Gene
- GenScript: Design optimal peptide antigens:
-
- Proteasome chain 3 (PSB4_HUMAN)
Graphical View of Domain Structure for InterPro Entry
P28070- Family:
-
- Belongs to the peptidase T1B family.
Function for PSMB4 Gene
Molecular function for PSMB4 Gene
- UniProtKB/Swiss-Prot Function:
- Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
- UniProtKB/Swiss-Prot Induction:
- Up-regulated in fibrolamellar carcinomas.
- GENATLAS Biochemistry:
- multicatalytic proteinase complex (20s),proteasome (prosome,macropain) component,beta 4 (HSBpros26),29.2kDa
Enzyme Numbers (IUBMB) for PSMB4 Gene
Phenotypes From GWAS Catalog for PSMB4 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0001530 | lipopolysaccharide binding | IEA | -- |
| GO:0004175 | endopeptidase activity | IBA | 21873635 |
| GO:0004298 | threonine-type endopeptidase activity | IEA | -- |
| GO:0005515 | protein binding | IPI | 15231748 |
| GO:0008233 | peptidase activity | IEA | -- |
Phenotypes for PSMB4 Gene
- GenomeRNAi human phenotypes for PSMB4:
-
- Increased vaccinia virus (VACV) infection
- Decreased viability
- no effect
- Synthetic lethal with Ras
- Decreased vaccinia virus (VACV) infection
- shRNA abundance <= 50%
- Reduced mammosphere formation
- Decreased NF-kappaB reporter expression
- shRNA abundance <= 25%
- Negative genetic interaction between PTEN-/- and PTEN+/+
- Decreased homologous recombination repair frequency
- Increased cell death in breast cancer cell lines (MCF10A, MDA-MB-435)
- Decreased viability of wild-type and TP53 knockout cells
- Decreased cell number, increased G2M DNA content
- Synthetic lethal with gemcitabine
- G2 arrest
Animal Models for research
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
CRISPR products for research
- Applied Biological Materials (abm): CRISPR Clones for PSMB4 - Starting at $85 >
- * PSMB4 CRISPR as ready-to-use vector or virus: Non-viral | Lenti- | Adeno- | AAV- | siRNA - Browse All
- * Cas9 Nucleases, Nickases, Null Mutants, and GFP tag also available.
-
OriGene CRISPR knockouts for PSMB4
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for PSMB4
-
Santa Cruz Biotechnology (SCBT) CRISPR for PSMB4
miRNA for PSMB4 Gene
- miRTarBase miRNAs that target PSMB4
miRNA products for research
Inhibitory RNAs for research
- Origene shrna, sirna, and RNAi products in human, mouse, rat for PSMB4
- Browse OriGene Inhibitory RNA Products For PSMB4
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PSMB4
Clone products for research
- Applied Biological Materials (abm): Clones for PSMB4 - Starting at $45 >
- * PSMB4 as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Retro- | Protein Vector - Browse All
- * Viral Packaging Services and High Titer Production Services also available.
- Sino Biological Human cDNA Clone for PSMB4
- Addgene plasmids for PSMB4
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for PSMB4
No data available for Animal Models , Transcription Factor Targets and HOMER Transcription for PSMB4 Gene
Localization for PSMB4 Gene
Subcellular locations from UniProtKB/Swiss-Prot for PSMB4 Gene
- Cytoplasm. Nucleus.
| Compartment | Confidence |
|---|---|
| cytoskeleton | 5 |
| nucleus | 5 |
| cytosol | 5 |
| extracellular | 4 |
| plasma membrane | 1 |
| mitochondrion | 1 |
| peroxisome | 1 |
| endoplasmic reticulum | 1 |
| endosome | 1 |
| lysosome | 1 |
| golgi apparatus | 1 |
- Nucleoplasm (3)
- Mitochondria (2)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000502 | proteasome complex | TAS,IDA | 17323924 |
| GO:0005634 | nucleus | IBA,TAS | 16130169 |
| GO:0005654 | nucleoplasm | TAS | -- |
| GO:0005737 | cytoplasm | IBA | 21873635 |
| GO:0005739 | mitochondrion | IDA | -- |
Pathways & Interactions for PSMB4 Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | CDK-mediated phosphorylation and removal of Cdc6 |
.93
.93
|
.80
.80
.78
.70
.47
.31
|
| 2 | RET signaling |
.92
|
.92
|
| 3 | Chks in Checkpoint Regulation |
Chks in Checkpoint Regulation
.59
G2-M Phase Transition
.59
Estrogen-mediated S-Phase Entry
.55
|
SREBP Proteolysis
.43
DNA Repair Mechanisms
.32
Parkinson's Disease Pathway
.31
|
| 4 | HIV Life Cycle |
.64
|
.45
|
| 5 | Pathways of neurodegeneration - multiple diseases | ||
Pathways by source for PSMB4 Gene
1 Sino Biological pathway for PSMB4 Gene
1 BioSystems pathway for PSMB4 Gene
8 KEGG pathways for PSMB4 Gene
4 GeneGo (Thomson Reuters) pathways for PSMB4 Gene
- Immune response Antigen presentation by MHC class I
- Regulation of degradation of deltaF508 CFTR in CF
- Regulation of degradation of wt-CFTR
- Transcription Ligand-Dependent Transcription of Retinoid-Target genes
17 Qiagen pathways for PSMB4 Gene
- CDK5 Pathway
- Chks in Checkpoint Regulation
- DNA Repair Mechanisms
- Estrogen-mediated S-Phase Entry
- G2-M Phase Transition
Interacting Proteins for PSMB4 Gene
Selected Interacting proteins:
P28070-PSB4_HUMAN
ENSP00000290541
for PSMB4 Gene via
UniProtKB
MINT
STRING
IID
GPS-Prot Interaction Network for PSMB4
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000165 | MAPK cascade | TAS | -- |
| GO:0000209 | protein polyubiquitination | TAS | -- |
| GO:0002223 | stimulatory C-type lectin receptor signaling pathway | TAS | -- |
| GO:0002479 | antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent | TAS | -- |
| GO:0002862 | negative regulation of inflammatory response to antigenic stimulus | IEA | -- |
No data available for SIGNOR curated interactions for PSMB4 Gene
Drugs & Compounds for PSMB4 Gene
| Name | Status | Disease Links | Group | Role | Mechanism of Action | Clinical Trials |
|---|---|---|---|---|---|---|
| Bortezomib | Approved, Investigational | Pharma | inhibitor, other | Proteosome and NF-kappaB inhibitor, Other, Proteasome inhibitors | 926 | |
| carfilzomib | Approved, Investigational | Pharma | other, Inhibitor | Other, 20S Proteasome Inhibitor | 0 | |
| (3AR,6R,6AS)-6-((S)-((S)-CYCLOHEX-2-ENYL)(HYDROXY)METHYL)-6A-METHYL-4-OXO-HEXAHYDRO-2H-FURO[3,2-C]PYRROLE-6-CARBALDEHYDE | Experimental | Pharma | Target | 0 |
Transcripts for PSMB4 Gene
mRNA/cDNA for PSMB4 Gene
- 1 REFSEQ mRNAs :
- 15 NCBI additional mRNA sequence :
- 7 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :
-
- ENST00000290541 918 nts (view in UCSC)
- ENST00000466425 665 nts (view in UCSC)
- ENST00000474100 755 nts (view in UCSC)
- ENST00000476467 2067 nts (view in UCSC)
- ENST00000493673 1650 nts (view in UCSC)
CRISPR products for research
- Applied Biological Materials (abm): CRISPR Clones for PSMB4 - Starting at $85 >
- * PSMB4 CRISPR as ready-to-use vector or virus: Non-viral | Lenti- | Adeno- | AAV- | siRNA - Browse All
- * Cas9 Nucleases, Nickases, Null Mutants, and GFP tag also available.
-
OriGene CRISPR knockouts for PSMB4
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for PSMB4