Aliases for PSMB4 Gene
Aliases for PSMB4 Gene
External Ids for PSMB4 Gene
- HGNC: 9541
- Entrez Gene: 5692
- Ensembl: ENSG00000159377
- OMIM: 602177
- UniProtKB: P28070
Previous GeneCards Identifiers for PSMB4 Gene
- GC01P149213
- GC01P147119
- GC01P148147
- GC01P148588
- GC01P148185
- GC01P149610
- GC01P122750
Summaries for PSMB4 Gene
-
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq, Jul 2008]
GeneCards Summary for PSMB4 Gene
PSMB4 (Proteasome Subunit Beta 4) is a Protein Coding gene. Diseases associated with PSMB4 include Fallopian Tube Carcinoma and Cystic Fibrosis. Among its related pathways are RET signaling and CDK-mediated phosphorylation and removal of Cdc6. Gene Ontology (GO) annotations related to this gene include threonine-type endopeptidase activity and lipopolysaccharide binding.
UniProtKB/Swiss-Prot for PSMB4 Gene
-
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Additional gene information for PSMB4 Gene
- Monarch Initiative
- Search for PSMB4 at DataMed
- Search for PSMB4 at HumanCyc
No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PSMB4 Gene
Genomics for PSMB4 Gene
GeneHancer (GH) Regulatory Elements for PSMB4 Gene
Promoters and enhancers for PSMB4 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data dump
GeneHancers around PSMB4 on UCSC Golden Path with GeneCards custom track
Regulatory Element Products
Genomic Locations for PSMB4 Gene
Genomic Locations for PSMB4 Gene
- chr1:151,399,534-151,401,944
- (GRCh38/hg38)
- Size:
- 2,411 bases
- Orientation:
- Plus strand
- chr1:151,372,010-151,374,420
- (GRCh37/hg19)
Genomic View for PSMB4 Gene
Genes around PSMB4 on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 1q21.3 by Ensembl
- 1q21.3 by Entrez Gene
- 1q21.3 by HGNC
PSMB4 Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for PSMB4 Gene
Proteins for PSMB4 Gene
-
Protein details for PSMB4 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- P28070-PSB4_HUMAN
- Recommended name:
- Proteasome subunit beta type-4
- Protein Accession:
- P28070
- B2R9L3
- P31148
- Q5SZS5
- Q6IBI4
- Q969L6
Protein attributes for PSMB4 Gene
- Size:
- 264 amino acids
- Molecular mass:
- 29204 Da
- Quaternary structure:
-
- The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. Interacts with HIV-1 TAT and HIV-1 NEF proteins (PubMed:14550573). Interaction with HTLV-1 TAX protein favors NFKB1 activation (PubMed:8692272). Forms a ternary complex with SMAD1 and OAZ1 before PSMB4 is incorporated into the 20S proteasome. Interacts with PRPF19 (PubMed:11571290, PubMed:12097147).
Protein Expression for PSMB4 Gene
Selected DME Specific Peptides for PSMB4 Gene
- P28070:
-
- LGASGDYAD
- QMVIDEEL
- RRSKMNPLWN
- LGSYGSL
- AIHSWLTRAMY
- GVVIAAD
- VLYYRDARSYNRF
- GESFLGYVD
- PITRTQNPMVTGTSVLG
Post-translational modifications for PSMB4 Gene
- Ubiquitination at Lys109
Other Protein References for PSMB4 Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibody Products
- Novus Biologicals Antibodies for PSMB4
-
Abcam antibodies for PSMB4
- Invitrogen Antibodies for PSMB4
- GeneTex PSMB4 antibody for PSMB4
-
Santa Cruz Biotechnology (SCBT) Antibodies for PSMB4
Protein Products
-
OriGene Purified Proteins for PSMB4
- Search Origene for MassSpec and Protein Over-expression Lysates for PSMB4
- Origene Custom Protein Services for PSMB4
- ProSpec Recombinant Proteins for PSMB4
- Search GeneTex for Proteins for PSMB4
-
Abcam proteins for PSMB4
Assay Products
Domains & Families for PSMB4 Gene
Gene Families for PSMB4 Gene
- HGNC:
- Human Protein Atlas (HPA):
-
- Enzymes
- Plasma proteins
- Predicted intracellular proteins
Protein Domains for PSMB4 Gene
- InterPro:
- Blocks:
- ProtoNet:
Suggested Antigen Peptide Sequences for PSMB4 Gene
- GenScript: Design optimal peptide antigens:
Graphical View of Domain Structure for InterPro Entry
P28070- Family:
-
- Belongs to the peptidase T1B family.
Function for PSMB4 Gene
Molecular function for PSMB4 Gene
- GENATLAS Biochemistry:
- multicatalytic proteinase complex (20s),proteasome (prosome,macropain) component,beta 4 (HSBpros26),29.2kDa
- UniProtKB/Swiss-Prot CatalyticActivity:
- Cleavage of peptide bonds with very broad specificity.
- UniProtKB/Swiss-Prot Function:
- Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
- UniProtKB/Swiss-Prot Induction:
- Up-regulated in fibrolamellar carcinomas.
Enzyme Numbers (IUBMB) for PSMB4 Gene
Phenotypes From GWAS Catalog for PSMB4 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0001530 | lipopolysaccharide binding | IEA | -- |
| GO:0004175 | endopeptidase activity | IEA | -- |
| GO:0004298 | threonine-type endopeptidase activity | IEA | -- |
| GO:0005515 | protein binding | IPI | 25416956 |
| GO:0008233 | peptidase activity | IEA | -- |
Phenotypes for PSMB4 Gene
- GenomeRNAi human phenotypes for PSMB4:
-
- Increased vaccinia virus (VACV) infection
- Decreased viability
- shRNA abundance <= 50%
- Synthetic lethal with Ras
- Decreased NF-kappaB reporter expression
- Negative genetic interaction between PTEN-/- and PTEN+/+
- Increased G1 DNA content
- G0/1 arrest
- Decreased homologous recombination repair frequency
- Increased cell death in breast cancer cell lines (MCF10A, MDA-MB-435)
- Decreased viability of wild-type and TP53 knockout cells
- Synthetic lethal with gemcitabine
Animal Model Products
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
- Cyagen custom Knockout/knockin (KOKI) mouse models for PSMB4
-
-
ViGene Biosciences lentiviral particle packaged cDNA for PSMB4 gene
-
ViGene Biosciences ready-to-package AAV shRNAs for PSMB4 gene
- Search ViGene Biosciences for PSMB4
CRISPR Products
-
OriGene CRISPR knockouts for PSMB4
- genomics-online: gRNA clones - Search results for available PSMB4 gene related products
- Applied Biological Materials CRISPR for PSMB4
-
Vectors and viruses for KO, Activation, Repression, and more
-
Santa Cruz Biotechnology (SCBT) CRISPR for PSMB4
- GenScript: Design CRISPR guide RNA sequences for PSMB4
miRNA for PSMB4 Gene
- miRTarBase miRNAs that target PSMB4
miRNA Products
- Search ViGene Biosciences for PSMB4
Inhibitory RNA Products
- Origene RNAi, sirna, and shrna products in human, mouse, rat for PSMB4
- Browse OriGene Inhibitory RNA Products For PSMB4
- genomics-online: shRNA clones - Search results for available PSMB4 gene related products
-
ViGene Biosciences ready-to-package AAV shRNAs for PSMB4 gene
Clone Products
- Sino Biological Human cDNA Clone for PSMB4
- Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat
- VectorBuilder custom plasmid, inducible vectors for PSMB4
- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for PSMB4
-
VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Addgene plasmids for PSMB4
- genomics-online: cdna clones - Search results for available PSMB4 gene related products
- orf clones - Search results for available PSMB4 gene related products
- Applied Biological Materials Clones for PSMB4
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
Cell Line Products
-
ViGene Biosciences adenoviral particle packaged cDNA for PSMB4 gene
-
ViGene Biosciences lentiviral particle packaged cDNA for PSMB4 gene
-
ViGene Biosciences ready-to-package AAV shRNAs for PSMB4 gene
No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for PSMB4 Gene
Localization for PSMB4 Gene
Subcellular locations from UniProtKB/Swiss-Prot for PSMB4 Gene
- Cytoplasm. Nucleus.
| Compartment | Confidence |
|---|---|
| extracellular | 5 |
| nucleus | 5 |
| cytosol | 5 |
| cytoskeleton | 2 |
| peroxisome | 1 |
- Centrosome (2)
- Cytosol (2)
- Intermediate filaments (2)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000502 | proteasome complex | TAS,IDA | 17323924 |
| GO:0005634 | nucleus | TAS | 16130169 |
| GO:0005654 | nucleoplasm | TAS | -- |
| GO:0005737 | cytoplasm | IEA | -- |
| GO:0005829 | cytosol | TAS | -- |
Pathways & Interactions for PSMB4 Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | CDK-mediated phosphorylation and removal of Cdc6 |
.93
.93
|
.80
.78
.78
.70
.47
.31
|
| 2 | RET signaling |
.92
|
.92
|
| 3 | Chks in Checkpoint Regulation | ||
| 4 | HIV Life Cycle |
.65
|
.45
|
| 5 | Class I MHC mediated antigen processing and presentation | ||
Pathways by source for PSMB4 Gene
1 Sino Biological pathway for PSMB4 Gene
1 BioSystems pathway for PSMB4 Gene
1 KEGG pathway for PSMB4 Gene
4 GeneGo (Thomson Reuters) pathways for PSMB4 Gene
17 Qiagen pathways for PSMB4 Gene
Interacting Proteins for PSMB4 Gene
Selected Interacting proteins:
ENSP00000290541
P28070-PSB4_HUMAN
for PSMB4 Gene via
STRING
MINT
UniProtKB
IID
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000165 | MAPK cascade | TAS | -- |
| GO:0000209 | protein polyubiquitination | TAS | -- |
| GO:0002223 | stimulatory C-type lectin receptor signaling pathway | TAS | -- |
| GO:0002479 | antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent | TAS | -- |
| GO:0002862 | negative regulation of inflammatory response to antigenic stimulus | IEA | -- |
No data available for SIGNOR curated interactions for PSMB4 Gene
Drugs & Compounds for PSMB4 Gene
| Name | Status | Disease Links | Group | Role | Mechanism of Action | Clinical Trials |
|---|---|---|---|---|---|---|
| Bortezomib | Approved, Investigational | Pharma | inhibitor, other | Proteosome and NF-kappaB inhibitor, Other, Proteasome inhibitors | 826 | |
| carfilzomib | Approved, Investigational | Pharma | other, Inhibitor | Other, 20S Proteasome Inhibitor | 0 | |
| (3AR,6R,6AS)-6-((S)-((S)-CYCLOHEX-2-ENYL)(HYDROXY)METHYL)-6A-METHYL-4-OXO-HEXAHYDRO-2H-FURO[3,2-C]PYRROLE-6-CARBALDEHYDE | Experimental | Pharma | Target | 0 |
Transcripts for PSMB4 Gene
mRNA/cDNA for PSMB4 Gene
- (1) REFSEQ mRNAs :
- (14) Additional mRNA sequences :
- (1191) Selected AceView cDNA sequences:
- (7) Ensembl transcripts including schematic representations, and UCSC links where relevant :
Unigene Clusters for PSMB4 Gene
CRISPR Products
-
OriGene CRISPR knockouts for PSMB4
- genomics-online: gRNA clones - Search results for available PSMB4 gene related products
- Applied Biological Materials CRISPR for PSMB4
-
Vectors and viruses for KO, Activation, Repression, and more
-
Santa Cruz Biotechnology (SCBT) CRISPR for PSMB4
- GenScript: Design CRISPR guide RNA sequences for PSMB4
miRNA Products
- Search ViGene Biosciences for PSMB4
Inhibitory RNA Products
- Origene RNAi, sirna, and shrna products in human, mouse, rat for PSMB4
- Browse OriGene Inhibitory RNA Products For PSMB4
- genomics-online: shRNA clones - Search results for available PSMB4 gene related products
-
ViGene Biosciences ready-to-package AAV shRNAs for PSMB4 gene
Clone Products
- Sino Biological Human cDNA Clone for PSMB4
- Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat
- VectorBuilder custom plasmid, inducible vectors for PSMB4
- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for PSMB4
-
VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Addgene plasmids for PSMB4
- genomics-online: cdna clones - Search results for available PSMB4 gene related products
- orf clones - Search results for available PSMB4 gene related products
- Applied Biological Materials Clones for PSMB4
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
| ExUns: | 1a | · | 1b | · | 1c | · | 1d | ^ | 2a | · | 2b | ^ | 3 | ^ | 4a | · | 4b | ^ | 5 | ^ | 6a | · | 6b | · | 6c | ^ | 7a | · | 7b | ^ | 8a | · | 8b | ^ | 9a | · | 9b |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | - | - | - | - | - | - | |||||||||||||||||||||||||||||||
| SP2: | - | - | - | - | - | ||||||||||||||||||||||||||||||||
| SP3: | - | - | |||||||||||||||||||||||||||||||||||
| SP4: | - | ||||||||||||||||||||||||||||||||||||
| SP5: | - | ||||||||||||||||||||||||||||||||||||
| SP6: | - | - | - | - | |||||||||||||||||||||||||||||||||
| SP7: | - | ||||||||||||||||||||||||||||||||||||
| SP8: | |||||||||||||||||||||||||||||||||||||
| SP9: | - |
Expression for PSMB4 Gene
This gene is overexpressed in Liver, secretome (6.8).
Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for PSMB4 Gene
- Elite partner
NURSA nuclear receptor signaling pathways regulating expression of PSMB4 Gene:
PSMB4SOURCE GeneReport for Unigene cluster for PSMB4 Gene:
Hs.89545Evidence on tissue expression from TISSUES for PSMB4 Gene
- Skin(4.9)
- Nervous system(4.9)
- Liver(4.7)
- Lung(4.3)
- Eye(3.7)
- Pancreas(3.3)
- Kidney(2.6)
- Bone(2.3)
- Intestine(2.2)
- Lymph node(2.2)
- Muscle(2.2)
- Bone marrow(2.1)
- Heart(2.1)
- Adrenal gland(2)
- Blood(2)
- Gall bladder(2)
- Spleen(2)
- Thyroid gland(2)
Primer Products
-
OriGene qPCR primer pairs for PSMB4
-
OriGene qPCR primer pairs and template standards for PSMB4
- genomics-online: primer clones - Search results for available PSMB4 gene related products
No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for PSMB4 Gene
Orthologs for PSMB4 Gene
This gene was present in the common ancestor of eukaryotes.
| Organism | Taxonomy | Gene | Similarity | Type | Details |
|---|---|---|---|---|---|
| chimpanzee (Pan troglodytes) |
Mammalia | PSMB4 33 34 |
|
||
| cow (Bos Taurus) |
Mammalia | PSMB4 33 34 |
|
||
| platypus (Ornithorhynchus anatinus) |
Mammalia | PSMB4 34 |
|
OneToOne | |
| dog (Canis familiaris) |
Mammalia | PSMB4 33 34 |
|
||
| mouse (Mus musculus) |
Mammalia | Psmb4 33 16 34 |
|
||
| rat (Rattus norvegicus) |
Mammalia | Psmb4 33 |
|
||
| oppossum (Monodelphis domestica) |
Mammalia | PSMB4 34 |
|
OneToOne | |
| chicken (Gallus gallus) |
Aves | PSMB4 33 34 |
|
||
| lizard (Anolis carolinensis) |
Reptilia | PSMB4 34 |
|
OneToOne | |
| tropical clawed frog (Silurana tropicalis) |
Amphibia | psmb4 33 |
|
||
| Str.129 33 |
|
||||
| African clawed frog (Xenopus laevis) |
Amphibia | psmb4-prov 33 |
|
||
| zebrafish (Danio rerio) |
Actinopterygii | psmb4 34 33 |
|
OneToOne | |
| Dr.15385 33 |
|
||||
| African malaria mosquito (Anopheles gambiae) |
Insecta | AgaP_AGAP011444 33 |
|
||
| fruit fly (Drosophila melanogaster) |
Insecta | Prosbeta7 |