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The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiq... See more...
Aliases for PSMB3 Gene
Aliases for PSMB3 Gene
External Ids for PSMB3 Gene
- HGNC: 9540
- Entrez Gene: 5691
- Ensembl: ENSG00000277791
- OMIM: 602176
- UniProtKB: P49720
Previous GeneCards Identifiers for PSMB3 Gene
- GC17P036394
- GC17P038902
- GC17P036819
- GC17P037284
- GC17P037285
- GC17P034162
- GC17P036909
- GC17P032705
Summaries for PSMB3 Gene
-
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The 26 S proteasome may be involved in trinucleotide repeat expansion, a phenomenon which is associated with many hereditary neurological diseases. Pseudogenes have been identified on chromosomes 2 and 12. Alternative splicing results in multiple transcript variants [provided by RefSeq, Sep 2013]
GeneCards Summary for PSMB3 Gene
PSMB3 (Proteasome 20S Subunit Beta 3) is a Protein Coding gene. Diseases associated with PSMB3 include Nodular Episcleritis and Cystic Fibrosis. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Antigen processing-Cross presentation. Gene Ontology (GO) annotations related to this gene include threonine-type endopeptidase activity. An important paralog of this gene is PSMB8.
UniProtKB/Swiss-Prot Summary for PSMB3 Gene
-
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Additional gene information for PSMB3 Gene
- HGNC(9540)
- Entrez Gene(5691)
- Ensembl(ENSG00000277791)
- OMIM(602176)
- UniProtKB(P49720)
- Open Targets Platform(ENSG00000277791)
- Search for PSMB3 at DataMed
- Search for PSMB3 at HumanCyc
No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for PSMB3 Gene
Genomics for PSMB3 Gene
GeneHancer (GH) Regulatory Elements Pubs
Promoters and enhancers for PSMB3 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data from 2017 publication |
Request up-to-date GeneHancer data (full dataset)
GeneHancers around PSMB3 on the GeneHancer Hub at the UCSC Golden Path
Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for PSMB3
- Top Transcription factor binding sites by QIAGEN in the PSMB3 gene promoter:
-
- GATA-3
- HOXA9
- HOXA9B
- LCR-F1
- Meis-1
- Meis-1b
- MyoD
- p300
- Pax-5
- YY1
Genomic Locations for PSMB3 Gene
Genomic Locations for PSMB3 Gene
- chr17:38,752,741-38,764,225
- (GRCh38/hg38)
- Size:
- 11,485 bases
- Orientation:
- Plus strand
- chr17:36,908,989-36,920,484
- (GRCh37/hg19)
- Size:
- 11,496 bases
- Orientation:
- Plus strand
Genomic View for PSMB3 Gene
Genes around PSMB3 on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 17q12 by HGNC
- 17q12 by Entrez Gene
- 17q12 by Ensembl
PSMB3 Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for PSMB3 Gene
Proteins for PSMB3 Gene
-
Protein details for PSMB3 Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- P49720-PSB3_HUMAN
- Recommended name:
- Proteasome subunit beta type-3
- Protein Accession:
- P49720
- P31147
- Q0P6J7
- Q96E27
Protein attributes for PSMB3 Gene
- Size:
- 205 amino acids
- Molecular mass:
- 22949 Da
- Quaternary structure:
-
- The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7.
- (Microbial infection) Interacts with HIV-1 TAT protein.
Protein Expression for PSMB3 Gene
Selected DME Specific Peptides for PSMB3 Gene
- P49720:
-
- EPVIAGLDPKTF
- GLATDVQT
- RFGIQAQ
- LYEKRFGP
- PFICSLDLIGCPMVT
- GKNCVAIA
- QRLKFRLNLYELKEGRQIKP
- LFETISQ
- CVAIAADRRFG
Post-translational modifications for PSMB3 Gene
Other Protein References for PSMB3 Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibody Products
- LifeSpan BioSciences PSMB3 Antibody
- Novus Biologicals Antibodies for PSMB3
-
Abcam antibodies for PSMB3
- Invitrogen Antibodies for PSMB3 (AFLGC-PSMB3)
-
Biorbyt antibodies for PSMB3
-
Boster Bio Antibodies for PSMB3
-
Santa Cruz Biotechnology (SCBT) Antibodies for PSMB3
Protein Products
-
OriGene Purified Proteins for PSMB3
- Search Origene for MassSpec and Protein Over-expression Lysates for PSMB3
- Origene Custom Protein Services for PSMB3
- ProSpec Recombinant Proteins for PSMB3
-
Signalway Proteins for PSMB3
-
Abcam proteins for PSMB3
Domains & Families for PSMB3 Gene
Gene Families for PSMB3 Gene
- HGNC:
- Human Protein Atlas (HPA):
-
- Enzymes
- Plasma proteins
- Predicted intracellular proteins
Protein Domains for PSMB3 Gene
- InterPro:
- Blocks:
-
- Proteasome B-type subunit
- ProtoNet:
Suggested Antigen Peptide Sequences for PSMB3 Gene
- GenScript: Design optimal peptide antigens:
-
- Proteasome theta chain (PSB3_HUMAN)
Graphical View of Domain Structure for InterPro Entry
P49720- Family:
-
- Belongs to the peptidase T1B family.
Function for PSMB3 Gene
Molecular function for PSMB3 Gene
- UniProtKB/Swiss-Prot Function:
- Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
- UniProtKB/Swiss-Prot CatalyticActivity:
- Reaction=Cleavage of peptide bonds with very broad specificity.; EC=3.4.25.1; Evidence={ECO:0000269|PubMed:27176742};.
- UniProtKB/Swiss-Prot Induction:
- Up-regulated in asthenozoospermic sperm.
- GENATLAS Biochemistry:
- multicatalytic proteinase complex (20s),proteasome component beta 3 (HSC10-I1),22.9kDa
Enzyme Numbers (IUBMB) for PSMB3 Gene
Phenotypes From GWAS Catalog for PSMB3 Gene
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0004175 | endopeptidase activity | IEA,IBA | 21873635 |
| GO:0004298 | threonine-type endopeptidase activity | IEA | -- |
| GO:0005515 | protein binding | IPI | 14733938 |
| GO:0008233 | peptidase activity | IEA | -- |
| GO:0016787 | hydrolase activity | IEA | -- |
Phenotypes for PSMB3 Gene
- GenomeRNAi human phenotypes for PSMB3:
-
- Decreased hepcidin::fluc mRNA expression
- no effect
- Decreased viability
- Decreased vaccinia virus (VACV) infection
- Dynamic nuclei (hole, folded or small irregular)
- Decreased Salmonella enterica Typhimurium invasion
- Decreased Salmonella enterica Typhimurium effector injection
- Decreased Salmonella enterica Typhimurium ruffling
- Negative genetic interaction between KRASG13D/+ and KRAS+/-
- shRNA abundance <= 25%
- Reduced mammosphere formation
- Decreased NF-kappaB reporter expression
- FOXO1 nuclear localization
- Decreased homologous recombination repair frequency
- HIV Rev nuclear localization
- Decreased shRNA abundance (Z-score < -2)
- Increased G2M DNA content
- Increased shRNA abundance (Z-score > 2)
- Decreased viability (cell death)
- Downregulation of NFkappaB pathway
- G2 arrest
Animal Model Products
- Taconic Biosciences: Generate A Custom CRISPR Mouse Model For Your Study
CRISPR Products
- Applied Biological Materials (abm): CRISPR Clones for PSMB3 - Select products 50% OFF >
- * PSMB3 CRISPR as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
-
OriGene CRISPR knockouts for PSMB3
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for PSMB3
-
Santa Cruz Biotechnology (SCBT) CRISPR for PSMB3
miRNA for PSMB3 Gene
- miRTarBase miRNAs that target PSMB3
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for PSMB3
- Browse OriGene Inhibitory RNA Products For PSMB3
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PSMB3
Clone Products
- Applied Biological Materials (abm): Clones for PSMB3 - Select products 50% OFF >
- * PSMB3 as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
- Sino Biological Human cDNA Clone for PSMB3
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for PSMB3
No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for PSMB3 Gene
Localization for PSMB3 Gene
Subcellular locations from UniProtKB/Swiss-Prot for PSMB3 Gene
- Cytoplasm. Nucleus.
| Compartment | Confidence |
|---|---|
| cytosol | 5 |
| extracellular | 4 |
| nucleus | 4 |
| mitochondrion | 3 |
| cytoskeleton | 2 |
| golgi apparatus | 2 |
| plasma membrane | 1 |
| peroxisome | 1 |
| endoplasmic reticulum | 1 |
| endosome | 1 |
| lysosome | 1 |
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000502 | proteasome complex | TAS,IDA | 17323924 |
| GO:0005634 | nucleus | IEA,IBA | 21873635 |
| GO:0005654 | nucleoplasm | TAS | -- |
| GO:0005737 | cytoplasm | IBA | 21873635 |
| GO:0005829 | cytosol | TAS | -- |
No data available for Subcellular locations from the Human Protein Atlas (HPA) for PSMB3 Gene
Pathways & Interactions for PSMB3 Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Regulation of activated PAK-2p34 by proteasome mediated degradation |
.93
.93
|
.80
.79
.78
.69
.47
|
| 2 | RET signaling |
.92
|
.92
|
| 3 | Chks in Checkpoint Regulation |
Chks in Checkpoint Regulation
.58
G2-M Phase Transition
.58
Estrogen-mediated S-Phase Entry
.54
|
SREBP Proteolysis
.42
DNA Repair Mechanisms
.31
Parkinson's Disease Pathway
.30
|
| 4 | HIV Life Cycle |
.65
|
.45
|
| 5 | Parkinson disease | ||
Pathways by source for PSMB3 Gene
1 Sino Biological pathway for PSMB3 Gene
1 BioSystems pathway for PSMB3 Gene
5 KEGG pathways for PSMB3 Gene
4 GeneGo (Thomson Reuters) pathways for PSMB3 Gene
- Immune response Antigen presentation by MHC class I
- Regulation of degradation of deltaF508 CFTR in CF
- Regulation of degradation of wt-CFTR
- Transcription Ligand-Dependent Transcription of Retinoid-Target genes
17 Qiagen pathways for PSMB3 Gene
- CDK5 Pathway
- Chks in Checkpoint Regulation
- DNA Repair Mechanisms
- Estrogen-mediated S-Phase Entry
- G2-M Phase Transition
Interacting Proteins for PSMB3 Gene
Selected Interacting proteins:
P49720-PSB3_HUMAN
ENSP00000483688
for PSMB3 Gene via
UniProtKB
MINT
STRING
IID
GPS-Prot Interaction Network for PSMB3
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0000165 | MAPK cascade | TAS | -- |
| GO:0000209 | protein polyubiquitination | TAS | -- |
| GO:0002223 | stimulatory C-type lectin receptor signaling pathway | TAS | -- |
| GO:0002479 | antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent | TAS | -- |
| GO:0006508 | proteolysis | IEA | -- |
No data available for SIGNOR curated interactions for PSMB3 Gene
Drugs & Compounds for PSMB3 Gene
| Name | Status | Disease Links | Group | Role | Mechanism of Action | Clinical Trials |
|---|---|---|---|---|---|---|
| Bortezomib | Approved, Investigational | Pharma | inhibitor, other | Proteosome and NF-kappaB inhibitor, Other, Proteasome inhibitors | 910 | |
| carfilzomib | Approved, Investigational | Pharma | other, Inhibitor | Other, 20S Proteasome Inhibitor | 0 | |
| (3AR,6R,6AS)-6-((S)-((S)-CYCLOHEX-2-ENYL)(HYDROXY)METHYL)-6A-METHYL-4-OXO-HEXAHYDRO-2H-FURO[3,2-C]PYRROLE-6-CARBALDEHYDE | Experimental | Pharma | Target | 0 |
Transcripts for PSMB3 Gene
mRNA/cDNA for PSMB3 Gene
- 1 REFSEQ mRNAs :
- 7 NCBI additional mRNA sequence :
- 6 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :
-
- ENST00000610434 510 nts (view in UCSC)
- ENST00000613870 662 nts (view in UCSC)
- ENST00000614132 426 nts (view in UCSC)
- ENST00000618159 562 nts (view in UCSC)
- ENST00000619426 762 nts (view in UCSC)
CRISPR Products
- Applied Biological Materials (abm): CRISPR Clones for PSMB3 - Select products 50% OFF >
- * PSMB3 CRISPR as ready-to-use vector or virus: ORF | Lenti- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
-
OriGene CRISPR knockouts for PSMB3
- Search GeneCopoeia for CRISPR/Cas9 clones, lentivirus and AAV products for PSMB3
-
Santa Cruz Biotechnology (SCBT) CRISPR for PSMB3
miRNA Products
Inhibitory RNA Products
- Origene shrna, sirna, and RNAi products in human, mouse, rat for PSMB3
- Browse OriGene Inhibitory RNA Products For PSMB3
- Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PSMB3
Clone Products
- Applied Biological Materials (abm): Clones for PSMB3 - Select products 50% OFF >
- * PSMB3 as ready-to-use vector or virus: ORF | Lenti- | Retro- | Adeno- | AAV- | Protein Vector - Browse All
- * Gene synthesis, site-directed mutagenesis, subcloning, and more services - Browse All
- Sino Biological Human cDNA Clone for PSMB3
- Search GeneCopoeia for ORF cDNA, lentivirus, AAV viral particles and/or promoter clone products for PSMB3
Relevant External Links for PSMB3 Gene
- GeneLoc Exon Structure for
- PSMB3
Expression for PSMB3 Gene
mRNA expression in embryonic tissues and stem cells from LifeMap Discovery
-
Kidney (Urinary System)
- Presumptive Podocytes Podocyte Layer
-
Epithelial Cells
- Presumptive Podocytes Podocyte Layer
- Brain (Nervous System)
- Intestine (Gastrointestinal Tract)
- Bone (Muscoskeletal System)
-
Uterus (Reproductive System)
-
Colon (Gastrointestinal Tract)
-
Prostate (Endocrine System)
-
Placenta (Extraembryonic Tissues)
-
Spleen (Hematopoietic System)
-
Thyroid (Endocrine System)
-
Gall Bladder (Hepatobiliary System)
-
Lung (Respiratory System)
-
Adrenal Gland (Endocrine System)
-
Esophagus (Gastrointestinal Tract)
-
Stomach (Gastrointestinal Tract)
-
Liver (Hepatobiliary System)
-
Adipose (Muscoskeletal System)