This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four tran... See more...

Aliases for PRSS3 Gene

Aliases for PRSS3 Gene

  • Serine Protease 3 2 3 4 5
  • Mesotrypsin 2 3 4
  • TRY3 2 3 4
  • TRY4 2 3 4
  • Protease, Serine, 4 (Trypsin 4, Brain) 2 3
  • Protease, Serine 3 2 3
  • Brain Trypsinogen 3 4
  • Mesotrypsinogen 3 4
  • Trypsin III 3 4
  • EC 3.4.21.4 4 51
  • Trypsin IV 3 4
  • Trypsin-3 3 4
  • PRSS4 3 4
  • Protease, Serine, 3 (Mesotrypsin) 2
  • Pancreatic Trypsinogen III 3
  • Serine Protease 4 4
  • Trypsinogen IV 3
  • Trypsinogen 4 3
  • Trypsinogen 5 3
  • EC 3.4.21 51
  • PRSS3 5
  • MTG 3
  • T9 3

External Ids for PRSS3 Gene

Previous HGNC Symbols for PRSS3 Gene

  • PRSS4

Previous GeneCards Identifiers for PRSS3 Gene

  • GC09P034062
  • GC09P033919
  • GC09P033740
  • GC09P033708

Summaries for PRSS3 Gene

Entrez Gene Summary for PRSS3 Gene

  • This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2010]

GeneCards Summary for PRSS3 Gene

PRSS3 (Serine Protease 3) is a Protein Coding gene. Diseases associated with PRSS3 include Thyroiditis and Rickettsialpox. Among its related pathways are Collagen chain trimerization and Defensins. Gene Ontology (GO) annotations related to this gene include calcium ion binding and serine-type peptidase activity. An important paralog of this gene is PRSS2.

UniProtKB/Swiss-Prot Summary for PRSS3 Gene

  • Digestive protease that cleaves proteins preferentially after an Arg residue and has proteolytic activity toward Kunitz-type trypsin inhibitors.

Additional gene information for PRSS3 Gene

No data available for CIViC Summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for PRSS3 Gene

Genomics for PRSS3 Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for PRSS3 Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around PRSS3 on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for PRSS3

Top Transcription factor binding sites by QIAGEN in the PRSS3 gene promoter:
  • AML1a
  • AREB6
  • C/EBPalpha
  • E47
  • ITF-2
  • Nkx5-1
  • Tal-1beta
  • Zic3

Genomic Locations for PRSS3 Gene

Genomic Locations for PRSS3 Gene
chr9:33,750,466-33,799,231
(GRCh38/hg38)
Size:
48,766 bases
Orientation:
Plus strand
chr9:33,750,464-33,799,230
(GRCh37/hg19)
Size:
48,767 bases
Orientation:
Plus strand

Genomic View for PRSS3 Gene

Genes around PRSS3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PRSS3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PRSS3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRSS3 Gene

Proteins for PRSS3 Gene

  • Protein details for PRSS3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P35030-TRY3_HUMAN
    Recommended name:
    Trypsin-3
    Protein Accession:
    P35030
    Secondary Accessions:
    • A8CED1
    • A8CED3
    • A9Z1Y4
    • E7ES07
    • F8W7P3
    • P15951
    • Q15665
    • Q5VXV0
    • Q6ISJ4
    • Q9UQV3

    Protein attributes for PRSS3 Gene

    Size:
    304 amino acids
    Molecular mass:
    32529 Da
    Cofactor:
    Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
    Quaternary structure:
    No Data Available

    Three dimensional structures from OCA and Proteopedia for PRSS3 Gene

    Alternative splice isoforms for PRSS3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PRSS3 Gene

Selected DME Specific Peptides for PRSS3 Gene

P35030:
  • IQVRLGE
  • GADYPDEL
  • DIMLIKL
  • DSGGPVVCNG
  • CLISGWG
  • CNGQLQG
  • SGWGNTLS
  • EGGKDSC
  • NDIMLIKL
  • GFLEGGKDSC
  • VRLGEHNI
  • FCGGSLI
  • PGVYTKV
  • HFCGGSL
  • VRLGEHN
  • LQGVVSWG
  • WVVSAAHC
  • WVVSAAHCY
  • DKIVGGY
  • PGVYTKVYNYV

Post-translational modifications for PRSS3 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Domains & Families for PRSS3 Gene

Gene Families for PRSS3 Gene

HGNC:
Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins
  • Predicted secreted proteins

Protein Domains for PRSS3 Gene

InterPro:
Blocks:
  • Serine protease, trypsin family
  • Chymotrypsin serine protease family (S1) signature
ProtoNet:

Suggested Antigen Peptide Sequences for PRSS3 Gene

GenScript: Design optimal peptide antigens:
  • PRSS3 protein (A1A508_HUMAN)
  • Trypsinogen 4 (A8CED1_HUMAN)
  • Trypsinogen 5 (A8CED3_HUMAN)
  • Protease, serine, 3 (Mesotrypsin), isoform CRA_b (Q6ISJ4_HUMAN)
  • PRSS3 protein (Q8N2U3_HUMAN)

Graphical View of Domain Structure for InterPro Entry

P35030

UniProtKB/Swiss-Prot:

TRY3_HUMAN :
  • Belongs to the peptidase S1 family.
Family:
  • Belongs to the peptidase S1 family.
genes like me logo Genes that share domains with PRSS3: view

Function for PRSS3 Gene

Molecular function for PRSS3 Gene

UniProtKB/Swiss-Prot Function:
Digestive protease that cleaves proteins preferentially after an Arg residue and has proteolytic activity toward Kunitz-type trypsin inhibitors.
UniProtKB/Swiss-Prot CatalyticActivity:
Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4; Evidence={ECO:0000269|PubMed:11827488, ECO:0000269|PubMed:14507909, ECO:0000269|PubMed:18077447, ECO:0000269|PubMed:25301953, ECO:0000269|PubMed:27810896, ECO:0000269|PubMed:6698368, ECO:0000269|PubMed:9099703};.
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:6698368};
UniProtKB/Swiss-Prot EnzymeRegulation:
Not inhibited by Kunitz-type trypsin inhibitors.
GENATLAS Biochemistry:
protease serine 3,trypsin 3,expressed in brain

Enzyme Numbers (IUBMB) for PRSS3 Gene

Phenotypes From GWAS Catalog for PRSS3 Gene

Gene Ontology (GO) - Molecular Function for PRSS3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004252 serine-type endopeptidase activity IBA,IDA 6698368
GO:0005509 calcium ion binding IDA 6698368
GO:0005515 protein binding IPI 6698368
GO:0008233 peptidase activity IEA --
GO:0008236 serine-type peptidase activity IEA --
genes like me logo Genes that share ontologies with PRSS3: view
genes like me logo Genes that share phenotypes with PRSS3: view

Animal Models for PRSS3 Gene

MGI Knock Outs for PRSS3:
  • Try4 Try4<tm1.1(KOMP)Vlcg>

Animal Model Products

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PRSS3

Clone Products

No data available for Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for PRSS3 Gene

Localization for PRSS3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRSS3 Gene

Secreted.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PRSS3 gene
Compartment Confidence
extracellular 5
mitochondrion 3
nucleus 3
cytosol 3
plasma membrane 2
cytoskeleton 2
endoplasmic reticulum 2
lysosome 2
peroxisome 1
endosome 1
golgi apparatus 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Endoplasmic reticulum (2)
  • Vesicles (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for PRSS3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region TAS --
GO:0005615 extracellular space IBA,IDA 6698368
GO:1904724 tertiary granule lumen TAS --
genes like me logo Genes that share ontologies with PRSS3: view

Pathways & Interactions for PRSS3 Gene

genes like me logo Genes that share pathways with PRSS3: view

Gene Ontology (GO) - Biological Process for PRSS3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006508 proteolysis TAS,NAS 8294000
GO:0007586 digestion IEA,TAS 6698368
GO:0009235 cobalamin metabolic process TAS --
GO:0019730 antimicrobial humoral response TAS --
GO:0031638 zymogen activation IDA 6698368
genes like me logo Genes that share ontologies with PRSS3: view

No data available for SIGNOR curated interactions for PRSS3 Gene

Drugs & Compounds for PRSS3 Gene

(14) Drugs for PRSS3 Gene - From: DrugBank, DGIdb, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
[4-(1,3,2-Dioxaborolan-2-Yloxy)Methyl]Benzamidine Experimental Pharma Target 0
1,3,2-Dioxaborolan-2-Ol Experimental Pharma Target 0
4-(1,3,2-Dioxaborolan-2-Yloxy)Butan-1-Aminium Experimental Pharma Target 0
4-(Hydroxymethyl)Benzamidine Experimental Pharma Target 0
4-Hydroxybutan-1-Aminium Experimental Pharma Target 0

(2) Additional Compounds for PRSS3 Gene - From: Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
genes like me logo Genes that share compounds with PRSS3: view

Transcripts for PRSS3 Gene

mRNA/cDNA for PRSS3 Gene

4 REFSEQ mRNAs :
10 NCBI additional mRNA sequence :
8 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

CRISPR Products

Inhibitory RNA Products

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for PRSS3

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for PRSS3 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7
SP1: - -
SP2: -
SP3:

Relevant External Links for PRSS3 Gene

GeneLoc Exon Structure for
PRSS3

Expression for PRSS3 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for PRSS3 Gene

mRNA differential expression in normal tissues according to GTEx for PRSS3 Gene

This gene is overexpressed in Pancreas (x45.7).

Protein differential expression in normal tissues from HIPED for PRSS3 Gene

This gene is overexpressed in Pancreatic juice (50.1) and Ovary (10.1).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for PRSS3 Gene



Transcriptomic regulation report from SPP (The Signaling Pathways Project) for PRSS3

SOURCE GeneReport for Unigene cluster for PRSS3 Gene:

Hs.654513

mRNA Expression by UniProt/SwissProt for PRSS3 Gene:

P35030-TRY3_HUMAN
Tissue specificity: Detected in pancreas and pancreatic fluid (at protein level) (PubMed:6698368). Expressed in pancreas and brain (PubMed:8294000). Detected in ileum (PubMed:12021776).

Evidence on tissue expression from TISSUES for PRSS3 Gene

  • Pancreas(4.9)
  • Nervous system(4.6)
  • Skin(3.1)
  • Intestine(2.7)
  • Blood(2.6)
  • Thyroid gland(2.3)
  • Muscle(2.3)
  • Heart(2.2)
  • Stomach(2.2)
  • Liver(2.1)
genes like me logo Genes that share expression patterns with PRSS3: view

No data available for Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for PRSS3 Gene

Orthologs for PRSS3 Gene

This gene was present in the common ancestor of animals.

Orthologs for PRSS3 Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia LOC100615846 30
  • 93.66 (n)
-- 31
  • 89 (a)
ManyToMany
Rat
(Rattus norvegicus)
Mammalia Try4 30
  • 82.93 (n)
Mouse
(Mus musculus)
Mammalia Try5 30 17 17 31
  • 82.52 (n)
ManyToMany
Try4 17 31
  • 76 (a)
ManyToMany
Prss1 31
  • 76 (a)
ManyToMany
Try10 31
  • 76 (a)
ManyToMany
Gm10334 31
  • 76 (a)
ManyToMany
Gm5771 31
  • 76 (a)
ManyToMany
Prss3 31
  • 75 (a)
ManyToMany
Prss2 31
  • 73 (a)
ManyToMany
Dog
(Canis familiaris)
Mammalia -- 31
  • 77 (a)
OneToMany
Cow
(Bos Taurus)
Mammalia PRSS2 31
  • 74 (a)
OneToMany
Oppossum
(Monodelphis domestica)
Mammalia -- 31
  • 73 (a)
ManyToMany
-- 31
  • 71 (a)
ManyToMany
-- 31
  • 68 (a)
ManyToMany
Platypus
(Ornithorhynchus anatinus)
Mammalia -- 31
  • 69 (a)
ManyToMany
-- 31
  • 67 (a)
ManyToMany
-- 31
  • 65 (a)
ManyToMany
-- 31
  • 64 (a)
ManyToMany
Chicken
(Gallus gallus)
Aves PRSS2 31
  • 66 (a)
ManyToMany
-- 31
  • 63 (a)
ManyToMany
PRSS3 31 31
  • 57 (a)
ManyToMany
-- 31
  • 53 (a)
ManyToMany
Lizard
(Anolis carolinensis)
Reptilia -- 31
  • 67 (a)
ManyToMany
-- 31
  • 63 (a)
ManyToMany
-- 31
  • 57 (a)
ManyToMany
-- 31
  • 42 (a)
ManyToMany
African clawed frog
(Xenopus laevis)
Amphibia MGC64344 30
Zebrafish
(Danio rerio)
Actinopterygii try 31
  • 64 (a)
OneToMany
Fruit Fly
(Drosophila melanogaster)
Insecta CG31954 30
  • 52.23 (n)
CG4386 32
  • 42 (a)
Try29F 32
  • 41 (a)
CG8299 32
  • 39 (a)
CG6041 32
  • 38 (a)
CG5197 32
  • 37 (a)
&lgr;Try 32
  • 37 (a)
CG17239 32
  • 36 (a)
CG17571 32
  • 36 (a)
&egr;Try 32
  • 36 (a)
CG7829 32
  • 33 (a)
&eegr;Try 32
  • 33 (a)
&kgr;Try 32
  • 33 (a)
Sea Squirt
(Ciona savignyi)
Ascidiacea CSA.4556 31
  • 44 (a)
ManyToMany
-- 31
  • 44 (a)
ManyToMany
CSA.4402 31
  • 43 (a)
ManyToMany
-- 31
  • 43 (a)
ManyToMany
-- 31
  • 42 (a)
ManyToMany
Species where no ortholog for PRSS3 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Eremothecium gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Baker's yeast (Saccharomyces cerevisiae)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Fission Yeast (Schizosaccharomyces pombe)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • K. Lactis Yeast (Kluyveromyces lactis)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rainbow Trout (Oncorhynchus mykiss)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Tropical Clawed Frog (Silurana tropicalis)
  • Wheat (Triticum aestivum)
  • Worm (Caenorhabditis elegans)

Evolution for PRSS3 Gene

ENSEMBL:
Gene Tree for PRSS3 (if available)
TreeFam:
Gene Tree for PRSS3 (if available)
Aminode:
Evolutionary constrained regions (ECRs) for PRSS3: view image

Paralogs for PRSS3 Gene

Paralogs for PRSS3 Gene

genes like me logo Genes that share paralogs with PRSS3: view

Variants for PRSS3 Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for PRSS3 Gene

SNP ID Clinical significance and condition Chr 09 pos Variation AA Info Type
rs1048379 - p.Tyr232Cys
rs1063273 - p.Thr224Ser
rs11547028 - p.Ala174Val
rs855581 - p.Thr188Ala

Additional dbSNP identifiers (rs#s) for PRSS3 Gene

Structural Variations from Database of Genomic Variants (DGV) for PRSS3 Gene

Variant ID Type Subtype PubMed ID
dgv7518n100 CNV gain 25217958
esv24699 CNV loss 19812545
esv2738377 CNV deletion 23290073
esv2738378 CNV deletion 23290073
esv2738379 CNV deletion 23290073
esv2761525 CNV gain 21179565
nsv1125318 OTHER inversion 24896259
nsv471653 CNV gain 15918152
nsv518977 CNV gain 19592680
nsv824910 CNV loss 20364138
nsv8444 CNV loss 18304495
nsv968607 CNV duplication 23825009
nsv968608 CNV duplication 23825009

Variation tolerance for PRSS3 Gene

Residual Variation Intolerance Score: 82.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.46; 77.40% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for PRSS3 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
PRSS3

SNP Genotyping and Copy Number Assay Products

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PRSS3 Gene

Disorders for PRSS3 Gene

MalaCards: The human disease database

(18) MalaCards diseases for PRSS3 Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
thyroiditis
rickettsialpox
  • rickettsia akari spotted fever
pancreatitis
hashimoto thyroiditis
  • ht
speech and communication disorders
  • communication disorder
- elite association - COSMIC cancer census association via MalaCards
Search PRSS3 in MalaCards View complete list of genes associated with diseases

Additional Disease Information for PRSS3

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology
Open Targets Platform
genes like me logo Genes that share disorders with PRSS3: view

No data available for UniProtKB/Swiss-Prot and Genatlas for PRSS3 Gene

Publications for PRSS3 Gene

  1. Keratinocytes synthesize enteropeptidase and multiple forms of trypsinogen during terminal differentiation. (PMID: 19924134) Nakanishi J … Hibino T (The Journal of investigative dermatology 2010) 3 4 23
  2. Genetic and biochemical characterization of the E32del polymorphism in human mesotrypsinogen. (PMID: 15855826) Nemoda Z … Sahin-Tóth M (Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2005) 3 23 41
  3. Human mesotrypsin is a unique digestive protease specialized for the degradation of trypsin inhibitors. (PMID: 14507909) Szmola R … Sahin-Tóth M (The Journal of biological chemistry 2003) 3 4 23
  4. Crystal structure reveals basis for the inhibitor resistance of human brain trypsin. (PMID: 11827488) Katona G … Szilágyi L (Journal of molecular biology 2002) 3 4 23
  5. Cloning of the cDNA encoding human brain trypsinogen and characterization of its product. (PMID: 8294000) Wiegand U … Müller-Hill B (Gene 1993) 2 3 4

Products for PRSS3 Gene

  • Boster Bio Antibodies for PRSS3
  • Boster Bio ELISA Kits for PRSS3

Sources for PRSS3 Gene