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Aliases for PRSS21 Gene

Aliases for PRSS21 Gene

  • Protease, Serine 21 2 3 5
  • Eosinophil Serine Protease 1 3 4
  • Testisin 2 3
  • ESP-1 3 4
  • TEST1 3 4
  • ESP1 3 4
  • Serine Protease From Eosinophils 3
  • Serine Protease 21 4
  • EC 3.4.21.- 4
  • EC 3.4.21 56

External Ids for PRSS21 Gene

Previous GeneCards Identifiers for PRSS21 Gene

  • GC16P002887
  • GC16P002867
  • GC16P002807
  • GC16P002833
  • GC16P002823
  • GC16P002834
  • GC16P002890
  • GC16P002940

Summaries for PRSS21 Gene

Entrez Gene Summary for PRSS21 Gene

  • This gene encodes a cell-surface anchored serine protease, which is a member of the trypsin family of serine proteases. The encoded protein is predicted to be active on peptide linkages involving the carboxyl group of lysine or arginine. The encoded protein localizes to the cytoplasm and the plasma membrane of premeiotic testicular germ cells and may be involved in progression of testicular tumors of germ cell origin. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

GeneCards Summary for PRSS21 Gene

PRSS21 (Protease, Serine 21) is a Protein Coding gene. Diseases associated with PRSS21 include Testicular Cancer and Dracunculiasis. Among its related pathways are Metabolism of proteins and Post-translational modification- synthesis of GPI-anchored proteins. Gene Ontology (GO) annotations related to this gene include serine-type endopeptidase activity and serine-type peptidase activity. An important paralog of this gene is PRSS41.

UniProtKB/Swiss-Prot for PRSS21 Gene

  • Could regulate proteolytic events associated with testicular germ cell maturation.

Additional gene information for PRSS21 Gene

No data available for CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PRSS21 Gene

Genomics for PRSS21 Gene

GeneHancer (GH) Regulatory Elements for PRSS21 Gene

Promoters and enhancers for PRSS21 Gene
GeneHancer (GH) Identifier GH Type GH
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH16I002816 Promoter/Enhancer 1.8 EPDnew Ensembl ENCODE 550.8 +0.4 363 1.7 HDAC1 ATF1 RB1 NFIB ZBTB40 EGR1 CREM SMARCC1 NCOA1 USF2 PRSS21 ZG16B PRSS41 EIF1P4
GH16I002799 Enhancer 1.1 Ensembl ENCODE 0.4 -17.2 -17212 1.7 PKNOX1 CLOCK FOXA2 NEUROD1 SIN3A FEZF1 ZNF2 ZNF548 FOS TSHZ1 GC16M002791 PRSS41 ZNF263 SRRM2 SNORA64 LOC105371055 ENSG00000255513 ZNF597 THOC6 E4F1
GH16I002822 Enhancer 0.5 ENCODE 0.4 +6.1 6053 0.8 CTCF ZNF654 SMC3 REST ZNF600 ZBTB33 RAD21 EIF1P4 ZG16B PRSS21
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around PRSS21 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the PRSS21 gene promoter:

Genomic Locations for PRSS21 Gene

Genomic Locations for PRSS21 Gene
9,142 bases
Plus strand

Genomic View for PRSS21 Gene

Genes around PRSS21 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PRSS21 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PRSS21 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PRSS21 Gene

Proteins for PRSS21 Gene

  • Protein details for PRSS21 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Protein Accession:
    Secondary Accessions:
    • Q9NS34
    • Q9P2V6

    Protein attributes for PRSS21 Gene

    314 amino acids
    Molecular mass:
    34884 Da
    Quaternary structure:
    No Data Available

    Alternative splice isoforms for PRSS21 Gene


neXtProt entry for PRSS21 Gene

Selected DME Specific Peptides for PRSS21 Gene


Post-translational modifications for PRSS21 Gene

  • Glycosylation at Asn167, isoforms=2, 3200, and Asn273

Domains & Families for PRSS21 Gene

Gene Families for PRSS21 Gene

Human Protein Atlas (HPA):
  • Enzymes
  • Plasma proteins
  • Predicted intracellular proteins
  • Predicted secreted proteins

Suggested Antigen Peptide Sequences for PRSS21 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the peptidase S1 family.
  • Belongs to the peptidase S1 family.
genes like me logo Genes that share domains with PRSS21: view

Function for PRSS21 Gene

Molecular function for PRSS21 Gene

GENATLAS Biochemistry:
protease serine 21,testisin,abundantly expressed in the testis and in testicular tumors,see TSG16D (role in progression of testicular tumor of germ cell origin)
UniProtKB/Swiss-Prot Function:
Could regulate proteolytic events associated with testicular germ cell maturation.

Enzyme Numbers (IUBMB) for PRSS21 Gene

Gene Ontology (GO) - Molecular Function for PRSS21 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004252 serine-type endopeptidase activity IEA --
GO:0005515 protein binding IPI 20211623
GO:0008233 peptidase activity IEA --
GO:0008236 serine-type peptidase activity IEA,TAS 10397266
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with PRSS21: view
genes like me logo Genes that share phenotypes with PRSS21: view

Animal Models for PRSS21 Gene

MGI Knock Outs for PRSS21:

Animal Model Products

Clone Products

No data available for Phenotypes From GWAS Catalog , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PRSS21 Gene

Localization for PRSS21 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PRSS21 Gene

Cell membrane; Lipid-anchor, GPI-anchor.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PRSS21 gene
Compartment Confidence
extracellular 5
plasma membrane 4
cytosol 2
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for PRSS21 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region TAS --
GO:0005737 cytoplasm TAS 10397266
GO:0005886 plasma membrane TAS --
GO:0016020 membrane TAS 9826525
GO:0031225 anchored component of membrane IEA --
genes like me logo Genes that share ontologies with PRSS21: view

Pathways & Interactions for PRSS21 Gene

genes like me logo Genes that share pathways with PRSS21: view

SIGNOR curated interactions for PRSS21 Gene


Gene Ontology (GO) - Biological Process for PRSS21 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006501 C-terminal protein lipidation TAS --
GO:0006508 proteolysis IEA --
GO:0007283 spermatogenesis IEA --
genes like me logo Genes that share ontologies with PRSS21: view

Drugs & Compounds for PRSS21 Gene

(2) Drugs for PRSS21 Gene - From: ApexBio and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
PMSF Pharma Serine proteinases inhibitor, irreversible 0

(1) ApexBio Compounds for PRSS21 Gene

Compound Action Cas Number
PMSF Serine proteinases inhibitor, irreversible 329-98-6
genes like me logo Genes that share compounds with PRSS21: view

Drug Products

Transcripts for PRSS21 Gene

Unigene Clusters for PRSS21 Gene

Protease, serine, 21 (testisin):
Representative Sequences:

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for PRSS21 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3a · 3b · 3c · 3d ^ 4a · 4b · 4c · 4d
SP1: - -
SP2: -
SP3: - - -
SP4: -
SP5: -

Relevant External Links for PRSS21 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for PRSS21 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for PRSS21 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for PRSS21 Gene

This gene is overexpressed in Testis (x34.4).

Protein differential expression in normal tissues from HIPED for PRSS21 Gene

This gene is overexpressed in Testis (40.7) and Ovary (28.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for PRSS21 Gene

Protein tissue co-expression partners for PRSS21 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of PRSS21 Gene:


SOURCE GeneReport for Unigene cluster for PRSS21 Gene:


mRNA Expression by UniProt/SwissProt for PRSS21 Gene:

Tissue specificity: Expressed predominantly in premeiotic testicular germ cells, mostly late pachytene and diplotene spermatocytes.

Evidence on tissue expression from TISSUES for PRSS21 Gene

  • Blood(4.2)
genes like me logo Genes that share expression patterns with PRSS21: view

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for PRSS21 Gene

Orthologs for PRSS21 Gene

This gene was present in the common ancestor of animals.

Orthologs for PRSS21 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia PRSS21 33 34
  • 99.28 (n)
(Canis familiaris)
Mammalia PRSS21 33
  • 76.21 (n)
-- 34
  • 25 (a)
(Bos Taurus)
Mammalia PRSS21 33 34
  • 74.43 (n)
(Rattus norvegicus)
Mammalia Prss21 33
  • 73.45 (n)
(Mus musculus)
Mammalia Prss21 33 16 34
  • 72.72 (n)
(Monodelphis domestica)
Mammalia -- 34
  • 40 (a)
-- 34
  • 38 (a)
-- 34
  • 35 (a)
(Anolis carolinensis)
Reptilia -- 34
  • 38 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG11824 35
  • 38 (a)
CG8170 35
  • 37 (a)
CG13744 35
  • 31 (a)
(Caenorhabditis elegans)
Secernentea ZK546.15 35
  • 33 (a)
Species where no ortholog for PRSS21 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • tropical clawed frog (Silurana tropicalis)
  • wheat (Triticum aestivum)
  • zebrafish (Danio rerio)

Evolution for PRSS21 Gene

Gene Tree for PRSS21 (if available)
Gene Tree for PRSS21 (if available)

Paralogs for PRSS21 Gene Pseudogenes for PRSS21 Gene

genes like me logo Genes that share paralogs with PRSS21: view

Variants for PRSS21 Gene

Sequence variations from dbSNP and Humsavar for PRSS21 Gene

SNP ID Clin Chr 16 pos Variation AA Info Type
rs1000507369 -- 2,820,072(+) A/G intron_variant
rs1000968942 -- 2,818,001(+) G/A/C intron_variant
rs1001195163 -- 2,816,839(+) G/A/C upstream_transcript_variant
rs1001349126 -- 2,820,643(+) G/A intron_variant
rs1002683412 -- 2,820,302(+) C/T intron_variant

Structural Variations from Database of Genomic Variants (DGV) for PRSS21 Gene

Variant ID Type Subtype PubMed ID
nsv952905 CNV deletion 24416366
esv2760067 CNV gain+loss 17122850

Variation tolerance for PRSS21 Gene

Residual Variation Intolerance Score: 61.7% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.99; 49.71% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for PRSS21 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PRSS21 Gene

Disorders for PRSS21 Gene

MalaCards: The human disease database

(2) MalaCards diseases for PRSS21 Gene - From: DISEASES and Novoseek

Disorder Aliases PubMed IDs
testicular cancer
  • childhood neoplasm of the testis
  • dracontiasis
- elite association - COSMIC cancer census association via MalaCards

Additional Disease Information for PRSS21

genes like me logo Genes that share disorders with PRSS21: view

No data available for UniProtKB/Swiss-Prot and Genatlas for PRSS21 Gene

Publications for PRSS21 Gene

  1. Testisin, a new human serine proteinase expressed by premeiotic testicular germ cells and lost in testicular germ cell tumors. (PMID: 10397266) Hooper JD … Antalis TM (Cancer research 1999) 2 3 4 22 58
  2. Localization, expression and genomic structure of the gene encoding the human serine protease testisin. (PMID: 11004480) Hooper JD … Antalis TM (Biochimica et biophysica acta 2000) 3 4 22 58
  3. Cloning and tissue distribution of a novel serine protease esp-1 from human eosinophils. (PMID: 9826525) Inoue M … Kido H (Biochemical and biophysical research communications 1998) 2 3 4 58
  4. The glycosylphosphatidylinositol-anchored serine protease PRSS21 (testisin) imparts murine epididymal sperm cell maturation and fertilizing ability. (PMID: 19571264) Netzel-Arnett S … Antalis TM (Biology of reproduction 2009) 3 22 58
  5. Epigenetic silencing of the putative tumor suppressor gene testisin in testicular germ cell tumors. (PMID: 16810501) Kempkensteffen C … Schrader M (Journal of cancer research and clinical oncology 2006) 3 22 58

Products for PRSS21 Gene

Sources for PRSS21 Gene

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