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Aliases for PPP1R8 Gene

Aliases for PPP1R8 Gene

  • Protein Phosphatase 1 Regulatory Subunit 8 2 2 3 5
  • Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 8 2 3
  • Nuclear Subunit Of PP-1 2 3
  • Activator Of RNA Decay 2 3
  • RNase E 2 3
  • NIPP-1 3 4
  • NIPP1 3 4
  • ARD1 3 4
  • Protein Phosphatase 1 Regulatory Inhibitor Subunit 8 4
  • Nuclear Inhibitor Of Protein Phosphatase-1 Alpha 3
  • Nuclear Inhibitor Of Protein Phosphatase-1 Beta 3
  • Protein Phosphatase 1, Regulatory Subunit 8 2
  • Nuclear Inhibitor Of Protein Phosphatase-1 2
  • Nuclear Inhibitor Of Protein Phosphatase 1 3
  • PRO2047 3
  • ARD-1 3

External Ids for PPP1R8 Gene

Previous GeneCards Identifiers for PPP1R8 Gene

  • GC01M027287
  • GC01P027131
  • GC01P027390
  • GC01P027761
  • GC01P027842
  • GC01P028029
  • GC01P028157
  • GC01P026413

Summaries for PPP1R8 Gene

Entrez Gene Summary for PPP1R8 Gene

  • This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008]

GeneCards Summary for PPP1R8 Gene

PPP1R8 (Protein Phosphatase 1 Regulatory Subunit 8) is a Protein Coding gene. Among its related pathways are Beta-Adrenergic Signaling and Activation of cAMP-Dependent PKA. Gene Ontology (GO) annotations related to this gene include RNA binding. An important paralog of this gene is SLC4A1AP.

UniProtKB/Swiss-Prot for PPP1R8 Gene

  • Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.

  • Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3 to purines and pyrimidines in A+U-rich regions. It generates 5-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.

Gene Wiki entry for PPP1R8 Gene

Additional gene information for PPP1R8 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for PPP1R8 Gene

Genomics for PPP1R8 Gene

GeneHancer (GH) Regulatory Elements for PPP1R8 Gene

Promoters and enhancers for PPP1R8 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH01I027829 Promoter/Enhancer 2.1 EPDnew Ensembl ENCODE 550.8 +0.8 814 3.7 HDGF PKNOX1 SMAD1 MLX ARNT ARID4B SIN3A ZNF2 YY1 POLR2B PPP1R8 YTHDF2 GMEB1 SNORA73B SNHG12 SNHG3 TRNAU1AP WASF2 RCC1 STX12
GH01I027487 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 30.4 -340.0 -340045 5.8 CLOCK MLX ZFP64 FEZF1 DMAP1 YY1 SLC30A9 ZNF416 ZNF143 MCM3 WASF2 ARID1A ACTG1P20 GPN2 FAM76A PHACTR4 NPM1P39 MAP3K6 ENSG00000231344 LOC101928728
GH01I027339 Promoter/Enhancer 2.2 EPDnew Ensembl ENCODE dbSUPER 25.8 -488.8 -488767 4.5 HDGF ARID4B SIN3A DMAP1 ZNF2 ZBTB7B ZNF213 ZNF143 FOS SP3 SYTL1 FAM76A PPP1R8 LOC101928324 LOC101928728 ENSG00000243659 ENSG00000231207 GPR3 ARID1A LIN28A
GH01I028258 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 20.1 +429.2 429227 3.7 HDGF PKNOX1 SMAD1 ARNT ARID4B SIN3A YY1 POLR2B ZNF766 ZNF213 SESN2 SRSF4 SNHG12 TRNAU1AP GMEB1 YTHDF2 PHACTR4 DNAJC8 ACTG1P20 ENSG00000270605
GH01I028647 Promoter/Enhancer 1.7 Ensembl ENCODE 25.4 +817.7 817704 2.7 CLOCK ZFP64 FEZF1 DMAP1 IRF4 YY1 SLC30A9 ZNF213 E2F8 ZNF143 ENSG00000270103 GC01P028651 GC01P028669 GC01P028670 GC01P028671 GC01P028672 RNU11 SRSF4 GMEB1 TRNAU1AP
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around PPP1R8 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the PPP1R8 gene promoter:

Genomic Locations for PPP1R8 Gene

Genomic Locations for PPP1R8 Gene
chr1:27,830,741-27,851,676
(GRCh38/hg38)
Size:
20,936 bases
Orientation:
Plus strand
chr1:28,157,252-28,178,187
(GRCh37/hg19)

Genomic View for PPP1R8 Gene

Genes around PPP1R8 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
PPP1R8 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for PPP1R8 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for PPP1R8 Gene

Proteins for PPP1R8 Gene

  • Protein details for PPP1R8 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q12972-PP1R8_HUMAN
    Recommended name:
    Nuclear inhibitor of protein phosphatase 1
    Protein Accession:
    Q12972
    Secondary Accessions:
    • Q5TEJ2
    • Q5TEJ4
    • Q5TIF2
    • Q6PKF6
    • Q9UBH1
    • Q9UBZ0

    Protein attributes for PPP1R8 Gene

    Size:
    351 amino acids
    Molecular mass:
    38479 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts with phosphorylated CDC5L, SF3B1 and MELK. Interacts with EED, in a nucleic acid-stimulated manner. Part of a complex consisting of PPP1R8, EED, HDAC2 and PP-1. Part of the spliceosome. Interacts with PPP1CA, PPP1CB and PPP1CC.
    Miscellaneous:
    • A synthetic peptide, NIPP-1(330-351), is able to inhibit PP-1. Phosphorylation of Tyr-335 reduces PP-1 inhibition, whereas phosphorylation of Thr-346 or Ser-348 has no effect.

    Three dimensional structures from OCA and Proteopedia for PPP1R8 Gene

    Alternative splice isoforms for PPP1R8 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for PPP1R8 Gene

Post-translational modifications for PPP1R8 Gene

  • May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA.

Other Protein References for PPP1R8 Gene

No data available for DME Specific Peptides for PPP1R8 Gene

Domains & Families for PPP1R8 Gene

Gene Families for PPP1R8 Gene

Protein Domains for PPP1R8 Gene

Graphical View of Domain Structure for InterPro Entry

Q12972

UniProtKB/Swiss-Prot:

PP1R8_HUMAN :
  • Has a basic N- and C-terminal and an acidic central domain.
Domain:
  • Has a basic N- and C-terminal and an acidic central domain.
  • The FHA domain mediates interactions with threonine-phosphorylated MELK.
genes like me logo Genes that share domains with PPP1R8: view

Function for PPP1R8 Gene

Molecular function for PPP1R8 Gene

GENATLAS Biochemistry:
protein phosphatase 1,regulatory (inhibitor) subunit 8,also encoding a site specific single strand endoribonuclease,functionally ressembling E Coli RNase E
UniProtKB/Swiss-Prot Function:
Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
UniProtKB/Swiss-Prot Function:
Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3 to purines and pyrimidines in A+U-rich regions. It generates 5-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.

Phenotypes From GWAS Catalog for PPP1R8 Gene

Gene Ontology (GO) - Molecular Function for PPP1R8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003677 DNA binding IEA --
GO:0003723 RNA binding TAS 9153239
GO:0003729 mRNA binding IBA --
GO:0004518 nuclease activity IEA --
GO:0004519 endonuclease activity IEA --
genes like me logo Genes that share ontologies with PPP1R8: view
genes like me logo Genes that share phenotypes with PPP1R8: view

Animal Models for PPP1R8 Gene

MGI Knock Outs for PPP1R8:

Animal Model Products

Inhibitory RNA Products

Clone Products

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for PPP1R8 Gene

Localization for PPP1R8 Gene

Subcellular locations from UniProtKB/Swiss-Prot for PPP1R8 Gene

Nucleus. Nucleus speckle. Note=Primarily, but not exclusively, nuclear.
Isoform Gamma: Cytoplasm. Note=Found mainly in the cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for PPP1R8 gene
Compartment Confidence
nucleus 5
cytosol 3
plasma membrane 1
cytoskeleton 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Nuclear speckles (4)
  • Nucleoplasm (4)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for PPP1R8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus TAS 7499293
GO:0005654 nucleoplasm IDA --
GO:0005681 spliceosomal complex IEA --
GO:0005737 cytoplasm IEA --
GO:0016607 nuclear speck IDA 11739654
genes like me logo Genes that share ontologies with PPP1R8: view

Pathways & Interactions for PPP1R8 Gene

genes like me logo Genes that share pathways with PPP1R8: view

Pathways by source for PPP1R8 Gene

Gene Ontology (GO) - Biological Process for PPP1R8 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IEA --
GO:0006355 regulation of transcription, DNA-templated IEA --
GO:0006397 mRNA processing IEA --
GO:0006401 RNA catabolic process TAS 9153239
GO:0008283 cell proliferation IEA --
genes like me logo Genes that share ontologies with PPP1R8: view

No data available for SIGNOR curated interactions for PPP1R8 Gene

Drugs & Compounds for PPP1R8 Gene

(2) Drugs for PPP1R8 Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Magnesium Approved Nutra 0

(1) Additional Compounds for PPP1R8 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Phosphate
  • NFB Orthophosphate
  • O-Phosphoric acid
  • Ortho-phosphate
  • Orthophosphate (PO43-)
  • Orthophosphate(3-)
14265-44-2
genes like me logo Genes that share compounds with PPP1R8: view

Transcripts for PPP1R8 Gene

Unigene Clusters for PPP1R8 Gene

Protein phosphatase 1, regulatory subunit 8:
Representative Sequences:

Inhibitory RNA Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for PPP1R8 Gene

ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b · 5c ^ 6a · 6b · 6c · 6d ^ 7 ^ 8
SP1: - - - -
SP2: - -
SP3: - - - - -
SP4: - - -
SP5: - - -
SP6: - -
SP7: - -

Relevant External Links for PPP1R8 Gene

GeneLoc Exon Structure for
PPP1R8
ECgene alternative splicing isoforms for
PPP1R8

Expression for PPP1R8 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for PPP1R8 Gene

Protein differential expression in normal tissues from HIPED for PPP1R8 Gene

This gene is overexpressed in CD8 Tcells (9.4) and Peripheral blood mononuclear cells (6.3).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for PPP1R8 Gene



Protein tissue co-expression partners for PPP1R8 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of PPP1R8 Gene:

PPP1R8

SOURCE GeneReport for Unigene cluster for PPP1R8 Gene:

Hs.533474

mRNA Expression by UniProt/SwissProt for PPP1R8 Gene:

Q12972-PP1R8_HUMAN
Tissue specificity: Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells.

Evidence on tissue expression from TISSUES for PPP1R8 Gene

  • Thyroid gland(4.5)
  • Blood(4.2)
  • Nervous system(4)
  • Kidney(2.7)
  • Adrenal gland(2.4)
  • Bone marrow(2.4)
  • Gall bladder(2.4)
  • Intestine(2.4)
  • Lung(2.4)
  • Lymph node(2.4)
  • Pancreas(2.4)
  • Skin(2.4)
  • Stomach(2.4)
genes like me logo Genes that share expression patterns with PPP1R8: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for PPP1R8 Gene

Orthologs for PPP1R8 Gene

This gene was present in the common ancestor of animals.

Orthologs for PPP1R8 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia PPP1R8 33 34
  • 99.53 (n)
oppossum
(Monodelphis domestica)
Mammalia PPP1R8 34
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia PPP1R8 33 34
  • 94.49 (n)
dog
(Canis familiaris)
Mammalia PPP1R8 33 34
  • 94.21 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia PPP1R8 34
  • 92 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ppp1r8 33
  • 91.17 (n)
mouse
(Mus musculus)
Mammalia Ppp1r8 33 16 34
  • 90.69 (n)
chicken
(Gallus gallus)
Aves PPP1R8 33 34
  • 80.44 (n)
lizard
(Anolis carolinensis)
Reptilia PPP1R8 34
  • 89 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia ppp1r8 33
  • 73.82 (n)
African clawed frog
(Xenopus laevis)
Amphibia MGC69160 33
zebrafish
(Danio rerio)
Actinopterygii ppp1r8b 34
  • 73 (a)
OneToMany
ppp1r8a 33 34
  • 71.37 (n)
-- 33
fruit fly
(Drosophila melanogaster)
Insecta NiPp1 33 34
  • 55.59 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP012089 33
  • 55.49 (n)
worm
(Caenorhabditis elegans)
Secernentea B0511.7 33 34
  • 53.28 (n)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.6111 33
Species where no ortholog for PPP1R8 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for PPP1R8 Gene

ENSEMBL:
Gene Tree for PPP1R8 (if available)
TreeFam:
Gene Tree for PPP1R8 (if available)

Paralogs for PPP1R8 Gene

Paralogs for PPP1R8 Gene

Pseudogenes.org Pseudogenes for PPP1R8 Gene

genes like me logo Genes that share paralogs with PPP1R8: view

Variants for PPP1R8 Gene

Sequence variations from dbSNP and Humsavar for PPP1R8 Gene

SNP ID Clin Chr 01 pos Variation AA Info Type
rs1000052398 -- 27,842,296(+) C/T intron_variant
rs1000187399 -- 27,841,887(+) T/C intron_variant
rs1000233580 -- 27,829,527(+) A/C upstream_transcript_variant
rs1000325780 -- 27,836,093(+) G/A intron_variant
rs1000452564 -- 27,829,387(+) C/G upstream_transcript_variant

Structural Variations from Database of Genomic Variants (DGV) for PPP1R8 Gene

Variant ID Type Subtype PubMed ID
nsv509069 CNV insertion 20534489

Variation tolerance for PPP1R8 Gene

Residual Variation Intolerance Score: 33.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.76; 33.32% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for PPP1R8 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
PPP1R8

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for PPP1R8 Gene

Disorders for PPP1R8 Gene

Additional Disease Information for PPP1R8

No disorders were found for PPP1R8 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for PPP1R8 Gene

Publications for PPP1R8 Gene

  1. ard-1: a human gene that reverses the effects of temperature-sensitive and deletion mutations in the Escherichia coli rne gene and encodes an activity producing RNase E-like cleavages. (PMID: 7524097) Wang M … Cohen SN (Proceedings of the National Academy of Sciences of the United States of America 1994) 2 3 4 22 58
  2. Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1. (PMID: 14699119) Vulsteke V … Bollen M (The Journal of biological chemistry 2004) 3 4 22 58
  3. Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1. (PMID: 12105215) Boudrez A … Bollen M (The Journal of biological chemistry 2002) 3 4 22 58
  4. Dynamic targeting of protein phosphatase 1 within the nuclei of living mammalian cells. (PMID: 11739654) Trinkle-Mulcahy L … Lamond AI (Journal of cell science 2001) 3 4 22 58
  5. Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1. (PMID: 11034904) Jagiello I … Bollen M (Journal of cell science 2000) 3 4 22 58

Products for PPP1R8 Gene

Sources for PPP1R8 Gene

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