This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional stu... See more...

Aliases for POLR3K Gene

Aliases for POLR3K Gene

  • RNA Polymerase III Subunit K 2 3 5
  • RPC11 2 3 4
  • Polymerase (RNA) III (DNA Directed) Polypeptide K, 12.3 KDa 2 3
  • DNA-Directed RNA Polymerase III Subunit RPC10 3 4
  • DNA-Directed RNA Polymerase III Subunit K 3 4
  • RNA Polymerase III 12.5 KDa Subunit 3 4
  • RNA Polymerase III Subunit C10 3 4
  • RNA Polymerase III Subunit C11 3 4
  • RPC12.5 3 4
  • C11 2 3
  • DNA-Directed RNA Polymerases III 12.5 KDa Polypeptide 3
  • RNA Polymerase III Subunit CII 3
  • Polymerase (RNA) III Subunit K 3
  • C11-RNP3 3
  • POLR3K 5
  • HsC11p 4
  • HRPC11 4
  • My010 3
  • RPC10 3

External Ids for POLR3K Gene

Previous GeneCards Identifiers for POLR3K Gene

  • GC16M000115
  • GC16M000036
  • GC16M000015
  • GC16M000096

Summaries for POLR3K Gene

Entrez Gene Summary for POLR3K Gene

  • This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.[provided by RefSeq, Jul 2010]

GeneCards Summary for POLR3K Gene

POLR3K (RNA Polymerase III Subunit K) is a Protein Coding gene. Diseases associated with POLR3K include Leukodystrophy, Hypomyelinating, 7, With Or Without Oligodontia And/Or Hypogonadotropic Hypogonadism and Leukodystrophy, Hypomyelinating, 8, With Or Without Oligodontia And/Or Hypogonadotropic Hypogonadism. Among its related pathways are Transcription of tRNA and RNA Polymerase III Transcription Initiation. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and RNA polymerase III activity. An important paralog of this gene is POLR2I.

UniProtKB/Swiss-Prot Summary for POLR3K Gene

  • DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).

Gene Wiki entry for POLR3K Gene

No data available for CIViC Summary , Tocris Summary , PharmGKB "VIP" Summary , Rfam classification and piRNA Summary for POLR3K Gene

Genomics for POLR3K Gene

GeneHancer (GH) Regulatory Elements Pubs

Promoters and enhancers for POLR3K Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH16J000052 Promoter/Enhancer 2 EPDnew Ensembl ENCODE CraniofacialAtlas 259.9 +0.4 407 3.6 SP1 ZNF207 NCOR1 MYC IKZF1 ZNF600 ZNF580 ZMYM3 ATF2 POLR2A SNRNP25 POLR3K lnc-RHBDF1-2 WDR90 WASH4P RHOT2 IL9RP3 CHTF18 ENSG00000268836 WDR24
GH16J000122 Enhancer 0.8 Ensembl ENCODE dbSUPER 5 -70.7 -70667 3.9 ZNF592 RELA HDAC2 CEBPB ZBTB20 ZBTB2 ENSG00000228779 NPRL3 SNRNP25 POLR3K RF00017-1709 MPG
GH16J000033 Promoter/Enhancer 1.3 FANTOM5 Ensembl ENCODE 0.4 +18.3 18291 1.8 CEBPA MBD2 CTBP1 TARDBP SIN3A JUND E4F1 BHLHE40 SUPT5H ZNF2 ENSG00000260803 LUC7L WDR90 WASH4P ENSG00000228779 LMF1 RHOT2 ENSG00000268836 WDR24 ENSG00000269482
GH16J000037 Enhancer 0.8 ENCODE 0.4 +15.1 15086 2.6 BCLAF1 SP1 CEBPB SIN3A TRIM22 ZNF217 ELF1 EBF1 CBFB SMARCA5 IL9RP3 SNRNP25 HSALNG0108691 POLR3K
GH16J000022 Promoter 0.9 EPDnew Ensembl 0.3 +30.5 30507 1 BHLHE40 CTCF EGR1 ZNF335 WASIR2 ENSG00000260803 POLR3K
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data from 2017 publication | Request up-to-date GeneHancer data (full dataset)

GeneHancers around POLR3K on the GeneHancer Hub at the UCSC Golden Path

Cistromic (ChIP-Seq) regulation report from SPP (The Signaling Pathways Project) for POLR3K

Top Transcription factor binding sites by QIAGEN in the POLR3K gene promoter:
  • AP-4
  • E2F
  • E2F-1
  • E2F-2
  • E2F-3a
  • E2F-4
  • p53

Genomic Locations for POLR3K Gene

Latest Assembly
chr16:46,407-53,608
(GRCh38/hg38)
Size:
7,202 bases
Orientation:
Minus strand

Previous Assembly
chr16:96,407-103,608
(GRCh37/hg19 by Entrez Gene)
Size:
7,202 bases
Orientation:
Minus strand

chr16:96,407-103,628
(GRCh37/hg19 by Ensembl)
Size:
7,222 bases
Orientation:
Minus strand

Genomic View for POLR3K Gene

Genes around POLR3K on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
POLR3K Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for POLR3K Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for POLR3K Gene

Proteins for POLR3K Gene

  • Protein details for POLR3K Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9Y2Y1-RPC10_HUMAN
    Recommended name:
    DNA-directed RNA polymerase III subunit RPC10
    Protein Accession:
    Q9Y2Y1
    Secondary Accessions:
    • Q1W6H4
    • Q96S35

    Protein attributes for POLR3K Gene

    Size:
    108 amino acids
    Molecular mass:
    12336 Da
    Quaternary structure:
    • Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.

neXtProt entry for POLR3K Gene

Post-translational modifications for POLR3K Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for POLR3K Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for POLR3K Gene

Domains & Families for POLR3K Gene

Gene Families for POLR3K Gene

HGNC:
Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for POLR3K Gene

InterPro:
Blocks:
  • DNA-directed RNA polymerase, M/15 kDa subunit

Suggested Antigen Peptide Sequences for POLR3K Gene

GenScript: Design optimal peptide antigens:
  • RNA polymerase III subunit C11 (RPC10_HUMAN)

Graphical View of Domain Structure for InterPro Entry

Q9Y2Y1

UniProtKB/Swiss-Prot:

RPC10_HUMAN :
  • Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
Family:
  • Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.
genes like me logo Genes that share domains with POLR3K: view

Function for POLR3K Gene

Molecular function for POLR3K Gene

UniProtKB/Swiss-Prot Function:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).

Phenotypes From GWAS Catalog for POLR3K Gene

Gene Ontology (GO) - Molecular Function for POLR3K Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003676 nucleic acid binding IEA --
GO:0003899 contributes_to DNA-directed 5'-3' RNA polymerase activity TAS,IBA 21873635
GO:0005515 protein binding IPI 32296183
GO:0008270 zinc ion binding IEA --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with POLR3K: view
genes like me logo Genes that share phenotypes with POLR3K: view

Animal Models for research

  • Taconic Biosciences Mouse Models for POLR3K

miRNA for POLR3K Gene

miRTarBase miRNAs that target POLR3K

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for POLR3K

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for POLR3K Gene

Localization for POLR3K Gene

Subcellular locations from UniProtKB/Swiss-Prot for POLR3K Gene

Nucleus, nucleolus.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for POLR3K gene
Compartment Confidence
nucleus 5
cytosol 4
extracellular 3
cytoskeleton 1
mitochondrion 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for POLR3K Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
GO:0005654 nucleoplasm TAS --
GO:0005666 RNA polymerase III complex TAS,IBA 21873635
GO:0005730 nucleolus IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with POLR3K: view

Pathways & Interactions for POLR3K Gene

genes like me logo Genes that share pathways with POLR3K: view

Pathways by source for POLR3K Gene

1 GeneGo (Thomson Reuters) pathway for POLR3K Gene
  • ATP/ITP metabolism
3 Qiagen pathways for POLR3K Gene
  • Assembly of RNA Polymerase-III Initiation Complex
  • DNA Repair Mechanisms
  • Transcription of tRNA

Gene Ontology (GO) - Biological Process for POLR3K Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002376 immune system process IEA --
GO:0006351 transcription, DNA-templated IEA --
GO:0006383 transcription by RNA polymerase III TAS 9869639
GO:0006386 termination of RNA polymerase III transcription IBA 21873635
GO:0032481 positive regulation of type I interferon production TAS --
genes like me logo Genes that share ontologies with POLR3K: view

No data available for SIGNOR curated interactions for POLR3K Gene

Drugs & Compounds for POLR3K Gene

(13) Drugs for POLR3K Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Cytidine triphosphate Experimental Pharma 0
Guanosine triphosphate Experimental Pharma 0
Phosphate Experimental Pharma 0
Uridine triphosphate Experimental, Investigational Pharma 0
ATP Investigational Nutra 0

(12) Additional Compounds for POLR3K Gene - From: HMDB and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
pyrophosphate
  • [(HO)2P(O)OP(O)(OH)2]
  • Acide diphosphorique
  • Diphosphorsaeure
  • H4P2O7
  • Pyrophosphoric acid
14000-31-8
genes like me logo Genes that share compounds with POLR3K: view

Transcripts for POLR3K Gene

mRNA/cDNA for POLR3K Gene

1 REFSEQ mRNAs :
4 NCBI additional mRNA sequence :
2 Ensembl transcripts including schematic representations, and UCSC links to gene/alias where relevant :

Inhibitory RNAs for research

  • Search GeneCopoeia for shRNA, lentivirus and/or AAV clone products for POLR3K

Alternative Splicing Database (ASD) splice patterns (SP) for POLR3K Gene

No ASD Table

Relevant External Links for POLR3K Gene

GeneLoc Exon Structure for
POLR3K

Expression for POLR3K Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for POLR3K Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for POLR3K Gene

This gene is overexpressed in Pancreas (34.7), Retina (21.3), and Fetal ovary (13.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for POLR3K Gene



Protein tissue co-expression partners for POLR3K Gene

Transcriptomic regulation report from SPP (The Signaling Pathways Project) for POLR3K

SOURCE GeneReport for Unigene cluster for POLR3K Gene:

Hs.632223

Evidence on tissue expression from TISSUES for POLR3K Gene

  • Nervous system(4.4)
genes like me logo Genes that share expression patterns with POLR3K: view

Primer products for research

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for POLR3K Gene

Orthologs for POLR3K Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for POLR3K Gene

Organism Taxonomy Gene Similarity Type Details
Chimpanzee
(Pan troglodytes)
Mammalia POLR3K 29 30
  • 99.07 (n)
OneToOne
Dog
(Canis familiaris)
Mammalia POLR3K 30
  • 99 (a)
OneToOne
Cow
(Bos Taurus)
Mammalia POLR3K 29 30
  • 91.67 (n)
OneToOne
Rat
(Rattus norvegicus)
Mammalia Polr3k 29
  • 91.05 (n)
Platypus
(Ornithorhynchus anatinus)
Mammalia POLR3K 30
  • 90 (a)
OneToOne
Mouse
(Mus musculus)
Mammalia Polr3k 29 16 30
  • 89.2 (n)
OneToOne
Chicken
(Gallus gallus)
Aves POLR3K 30
  • 87 (a)
OneToOne
Lizard
(Anolis carolinensis)
Reptilia POLR3K 30
  • 83 (a)
OneToOne
Tropical Clawed Frog
(Silurana tropicalis)
Amphibia polr3k 29
  • 76.23 (n)
Str.787 29
African clawed frog
(Xenopus laevis)
Amphibia MGC68582 29
Zebrafish
(Danio rerio)
Actinopterygii polr3k 29 30
  • 76.23 (n)
OneToOne
Dr.24992 29
Rainbow Trout
(Oncorhynchus mykiss)
Actinopterygii Omy.9441 29
Fruit Fly
(Drosophila melanogaster)
Insecta CG33785 29 30
  • 66.05 (n)
OneToOne
Worm
(Caenorhabditis elegans)
Secernentea Y77E11A.6 29 31
  • 62.35 (n)
rpc-11 30
  • 62 (a)
OneToOne
A. gosspyii yeast
(Eremothecium gossypii)
Saccharomycetes AGOS_AGR029W 29
  • 61.95 (n)
K. Lactis Yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0F18568g 29
  • 59.12 (n)
Baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RPC11 29 30 32
  • 55.77 (n)
OneToOne
Sea Squirt
(Ciona savignyi)
Ascidiacea -- 30
  • 79 (a)
OneToOne
Fission Yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes rpc11 29
  • 53.72 (n)
Bread mold
(Neurospora crassa)
Ascomycetes NCU00570 29
  • 52.98 (n)
Species where no ortholog for POLR3K was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • Alpha proteobacteria (Wolbachia pipientis)
  • Amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • Barley (Hordeum vulgare)
  • Beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • Common water flea (Daphnia pulex)
  • Corn (Zea mays)
  • E. coli (Escherichia coli)
  • Filamentous fungi (Aspergillus nidulans)
  • Firmicute Bacteria (Streptococcus pneumoniae)
  • Green Algae (Chlamydomonas reinhardtii)
  • Honey Bee (Apis mellifera)
  • Loblloly Pine (Pinus taeda)
  • Malaria Parasite (Plasmodium falciparum)
  • Medicago Trunc (Medicago Truncatula)
  • Moss (Physcomitrella patens)
  • Oppossum (Monodelphis domestica)
  • Orangutan (Pongo pygmaeus)
  • Pig (Sus scrofa)
  • Rice (Oryza sativa)
  • Rice Blast Fungus (Magnaporthe grisea)
  • Schistosome Parasite (Schistosoma mansoni)
  • Sea Anemone (Nematostella vectensis)
  • Sea Vase (Ciona intestinalis)
  • Sea Urchin (Strongylocentrotus purpuratus)
  • Sorghum (Sorghum bicolor)
  • Soybean (Glycine max)
  • Stem Rust Fungus (Puccinia graminis)
  • Sugarcane (Saccharum officinarum)
  • Thale Cress (Arabidopsis thaliana)
  • Tomato (Lycopersicon esculentum)
  • Toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • Wheat (Triticum aestivum)

Evolution for POLR3K Gene

ENSEMBL:
Gene Tree for POLR3K (if available)
TreeFam:
Gene Tree for POLR3K (if available)
Aminode:
Evolutionary constrained regions (ECRs) for POLR3K: view image
Alliance of Genome Resources:
Additional Orthologs for POLR3K

Paralogs for POLR3K Gene

Paralogs for POLR3K Gene

Pseudogenes.org Pseudogenes for POLR3K Gene

genes like me logo Genes that share paralogs with POLR3K: view

Variants for POLR3K Gene

Sequence variations, with clinical significance, from ClinVar and Humsavar, with links to dbSNP for POLR3K Gene

SNP ID Clinical significance and condition Chr 16 pos Variation AA Info Type
rs183360 -- p.Ser24Ala

dbSNP identifiers (rs#s) for variants without ClinVar clinical significance for POLR3K Gene

All consequence types are included: molecular consequences (e.g. missense, synonymous), and location-based (e.g. intron, upstream).

Structural Variations from Database of Genomic Variants (DGV) for POLR3K Gene

Variant ID Type Subtype PubMed ID
dgv2688n100 CNV gain 25217958
dgv2689n100 CNV gain 25217958
dgv2690n100 CNV gain 25217958
esv2422427 CNV duplication 17116639
esv2760065 CNV gain 17122850
esv3637535 CNV loss 21293372
nsv1037456 CNV gain+loss 25217958
nsv1044142 CNV gain 25217958
nsv1044732 CNV loss 25217958
nsv1045829 CNV gain 25217958
nsv518291 CNV loss 19592680
nsv522688 CNV loss 19592680

Variation tolerance for POLR3K Gene

Residual Variation Intolerance Score: 48.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.38; 8.33% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for POLR3K Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
POLR3K
Leiden Open Variation Database (LOVD)
POLR3K

SNP Genotyping and Copy Number Assays for research

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for POLR3K Gene

Disorders for POLR3K Gene

MalaCards: The human disease database

(6) MalaCards diseases for POLR3K Gene - From: COP

Additional Disease Information for POLR3K

genes like me logo Genes that share disorders with POLR3K: view

No data available for UniProtKB/Swiss-Prot and Genatlas for POLR3K Gene

Publications for POLR3K Gene

  1. [Molecular identification and characteristics of hRPC11, the smallest specific subunit of human RNA polymerase III]. (PMID: 10079944) Spakovskiĭ GV … Lebedenko EN (Bioorganicheskaia khimiia 1998) 2 3 4 22
  2. Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits. (PMID: 12391170) Hu P … Hernandez N (Molecular and cellular biology 2002) 3 4 22
  3. The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination. (PMID: 9869639) Chédin S … Carles C (Genes & development 1998) 2 3 4
  4. RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate. (PMID: 19609254) Ablasser A … Hornung V (Nature immunology 2009) 4 22
  5. A regulatory SNP causes a human genetic disease by creating a new transcriptional promoter. (PMID: 16728641) De Gobbi M … Higgs DR (Science (New York, N.Y.) 2006) 3 4

Products for POLR3K Gene

Sources for POLR3K Gene